YOR251C / TUM1

Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR251C / TUM1

Click on Significant Values for Screen Details ID:SGTC_112|Compound:phenothiazine|FD-Score:3.39|P-value:6.84E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.04|P-value:3.13E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:2.88|P-value:5.81E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.11|P-value:3.08E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.11|P-value:2.28E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.16|P-value:2.50E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.11|P-value:2.29E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.92|P-value:5.46E-6 ID:SGTC_359|Compound:0081-0058|FD-Score:4.69|P-value:7.10E-8 ID:SGTC_378|Compound:3970-0959|FD-Score:3.59|P-value:2.74E-5 ID:SGTC_393|Compound:acivicin|FD-Score:-4.16|P-value:2.14E-6 ID:SGTC_442|Compound:cape|FD-Score:3.10|P-value:2.37E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-3.01|P-value:4.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.87|P-value:9.82E-6 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.05|P-value:2.64E-6 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.05|P-value:2.97E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:2.89|P-value:5.67E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.54|P-value:3.45E-5 ID:SGTC_684|Compound:1140-0066|FD-Score:3.01|P-value:3.55E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.50|P-value:4.23E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:4.92|P-value:1.69E-8 ID:SGTC_800|Compound:3448-7362|FD-Score:2.76|P-value:9.43E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.88|P-value:5.89E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.30|P-value:1.03E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.91|P-value:5.67E-6 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.01|P-value:4.64E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:2.91|P-value:5.13E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:2.81|P-value:7.69E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.76|P-value:4.69E-8 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.21|P-value:1.54E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.12|P-value:2.17E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.30|P-value:1.03E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.82|P-value:9.65E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.46|P-value:5.08E-5 ID:SGTC_1670|Compound:st016595|FD-Score:2.94|P-value:4.60E-4 ID:SGTC_1720|Compound:st037298|FD-Score:3.36|P-value:7.96E-5 ID:SGTC_1765|Compound:st044815|FD-Score:-3.01|P-value:4.56E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.26|P-value:1.20E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.95|P-value:4.50E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.56|P-value:3.20E-5 ID:SGTC_2142|Compound:5622915|FD-Score:-3.12|P-value:3.00E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.51|P-value:3.89E-5 ID:SGTC_2156|Compound:5649887|FD-Score:3.96|P-value:4.33E-6 ID:SGTC_2158|Compound:5657123|FD-Score:3.26|P-value:1.23E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.27|P-value:8.08E-7 ID:SGTC_2288|Compound:7946289|FD-Score:3.34|P-value:8.61E-5 ID:SGTC_2307|Compound:7538926|FD-Score:2.88|P-value:5.85E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.56|P-value:3.17E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.59|P-value:2.76E-5 ID:SGTC_2324|Compound:6149169|FD-Score:-2.86|P-value:8.13E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.78|P-value:8.52E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.36|P-value:7.94E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:2.83|P-value:7.11E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.27|P-value:1.59E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:2.83|P-value:7.21E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:2.97|P-value:4.11E-4 ID:SGTC_2624|Compound:nerol|FD-Score:4.81|P-value:3.47E-8 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-4.73|P-value:8.42E-8 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.65|P-value:2.88E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.57|P-value:2.92E-5 ID:SGTC_2836|Compound:9010338|FD-Score:2.94|P-value:4.59E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.32|P-value:9.55E-5 ID:SGTC_3079|Compound:9117512|FD-Score:3.32|P-value:9.28E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.39|P-value:6.84E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.04|P-value:3.13E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:2.88|P-value:5.81E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.11|P-value:3.08E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.11|P-value:2.28E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.16|P-value:2.50E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.11|P-value:2.29E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.92|P-value:5.46E-6 ID:SGTC_359|Compound:0081-0058|FD-Score:4.69|P-value:7.10E-8 ID:SGTC_378|Compound:3970-0959|FD-Score:3.59|P-value:2.74E-5 ID:SGTC_393|Compound:acivicin|FD-Score:-4.16|P-value:2.14E-6 ID:SGTC_442|Compound:cape|FD-Score:3.10|P-value:2.37E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-3.01|P-value:4.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.87|P-value:9.82E-6 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.05|P-value:2.64E-6 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.05|P-value:2.97E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:2.89|P-value:5.67E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.54|P-value:3.45E-5 ID:SGTC_684|Compound:1140-0066|FD-Score:3.01|P-value:3.55E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.50|P-value:4.23E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:4.92|P-value:1.69E-8 ID:SGTC_800|Compound:3448-7362|FD-Score:2.76|P-value:9.43E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.88|P-value:5.89E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.30|P-value:1.03E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.91|P-value:5.67E-6 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.01|P-value:4.64E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:2.91|P-value:5.13E-4 ID:SGTC_1114|Compound:scopoletin|FD-Score:2.81|P-value:7.69E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.76|P-value:4.69E-8 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.21|P-value:1.54E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.12|P-value:2.17E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.30|P-value:1.03E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.82|P-value:9.65E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.46|P-value:5.08E-5 ID:SGTC_1670|Compound:st016595|FD-Score:2.94|P-value:4.60E-4 ID:SGTC_1720|Compound:st037298|FD-Score:3.36|P-value:7.96E-5 ID:SGTC_1765|Compound:st044815|FD-Score:-3.01|P-value:4.56E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.26|P-value:1.20E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.95|P-value:4.50E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.56|P-value:3.20E-5 ID:SGTC_2142|Compound:5622915|FD-Score:-3.12|P-value:3.00E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.51|P-value:3.89E-5 ID:SGTC_2156|Compound:5649887|FD-Score:3.96|P-value:4.33E-6 ID:SGTC_2158|Compound:5657123|FD-Score:3.26|P-value:1.23E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.27|P-value:8.08E-7 ID:SGTC_2288|Compound:7946289|FD-Score:3.34|P-value:8.61E-5 ID:SGTC_2307|Compound:7538926|FD-Score:2.88|P-value:5.85E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.56|P-value:3.17E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.59|P-value:2.76E-5 ID:SGTC_2324|Compound:6149169|FD-Score:-2.86|P-value:8.13E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.78|P-value:8.52E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.36|P-value:7.94E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:2.83|P-value:7.11E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.27|P-value:1.59E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:2.83|P-value:7.21E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:2.97|P-value:4.11E-4 ID:SGTC_2624|Compound:nerol|FD-Score:4.81|P-value:3.47E-8 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-4.73|P-value:8.42E-8 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.65|P-value:2.88E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.57|P-value:2.92E-5 ID:SGTC_2836|Compound:9010338|FD-Score:2.94|P-value:4.59E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.32|P-value:9.55E-5 ID:SGTC_3079|Compound:9117512|FD-Score:3.32|P-value:9.28E-5

Top fitness defect scores for YOR251C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_786 4544-0069 4.92 423.0 µM 1.69E-8 13 4.26E-7
2 SGTC_2624 nerol 4.81 100.0 µM 3.47E-8 4 7.61E-7
3 SGTC_1153 4055-0057 4.76 267.0 µM 4.69E-8 7 9.70E-7
4 SGTC_359 0081-0058 4.69 15.9 µM 7.10E-8 60S ribosome export 25 1.35E-6
5 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 4.27 122.8 µM 8.08E-7 4 9.65E-6
6 SGTC_474 dl-dihydrosphingosine 4.05 5.0 µM 2.64E-6 24 2.51E-5
7 SGTC_2156 5649887 3.96 115.0 µM 4.33E-6 RPP1 & pyrimidine depletion 10 3.75E-5
8 SGTC_271 rotenone 3.92 740.0 µM 5.46E-6 25 4.52E-5
9 SGTC_1002 4049-0206 3.91 172.0 µM 5.67E-6 34 4.66E-5
10 SGTC_378 3970-0959 3.59 8.3 µM 2.74E-5 25 1.66E-4
11 SGTC_2321 7390090 3.59 138.8 µM 2.76E-5 38 1.67E-4
12 SGTC_2679 rapamycin 3.57 1.0 nM 2.92E-5 172 1.75E-4
13 SGTC_2318 6029776 3.56 164.0 µM 3.17E-5 44 1.87E-4
14 SGTC_2133 5320837 3.56 200.0 µM 3.20E-5 ERG2 38 1.88E-4
15 SGTC_654 1124-0271 3.54 80.9 µM 3.45E-5 41 2.00E-4
16 SGTC_2148 5546263 3.51 113.6 µM 3.89E-5 30 2.21E-4
17 SGTC_723 4182-0001 3.50 115.0 µM 4.23E-5 37 2.37E-4
18 SGTC_1295 1013-0063 3.46 9.8 µM 5.08E-5 26 2.74E-4
19 SGTC_112 phenothiazine 3.39 287.2 µM 6.84E-5 unfolded protein response 26 3.49E-4
20 SGTC_2455 5377712 3.36 9.8 µM 7.94E-5 39 3.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.13E-18 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.142 1.72E-16 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.137 1.55E-15 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.132 1.42E-14 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.130 4.75E-14 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.128 1.22E-13 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
0.126 2.27E-13 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.123 7.78E-13 YIL017C VID28 hom GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm
0.121 2.07E-12 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.120 3.19E-12 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.119 4.36E-12 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.110 1.44E-10 YIL008W URM1 hom Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
0.110 1.54E-10 YBL089W AVT5 hom Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
0.109 2.54E-10 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.109 2.64E-10 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB