YOR258W / HNT3

DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR258W / HNT3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.12|P-value:4.08E-8 ID:SGTC_114|Compound:0364-0001|FD-Score:3.67|P-value:6.51E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:5.06|P-value:4.78E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:7.56|P-value:1.78E-11 ID:SGTC_138|Compound:0850-0259|FD-Score:10.60|P-value:8.02E-21 ID:SGTC_197|Compound:4544-0043|FD-Score:3.83|P-value:3.98E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.78|P-value:4.63E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.89|P-value:3.32E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.63|P-value:7.35E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.64|P-value:7.28E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.28|P-value:1.91E-6 ID:SGTC_446|Compound:go6976|FD-Score:6.63|P-value:3.14E-9 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.85|P-value:1.07E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.19|P-value:2.77E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:7.25|P-value:1.04E-10 ID:SGTC_521|Compound:r062-0005|FD-Score:3.71|P-value:5.80E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.21|P-value:1.15E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:4.16|P-value:1.37E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:4.85|P-value:1.11E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:7.07|P-value:2.94E-10 ID:SGTC_612|Compound:0302-0167|FD-Score:4.69|P-value:2.05E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:4.00|P-value:2.35E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:5.12|P-value:3.67E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:10.40|P-value:3.67E-20 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.26|P-value:9.65E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:7.52|P-value:2.26E-11 ID:SGTC_829|Compound:1635-0120|FD-Score:4.29|P-value:8.61E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:9.08|P-value:8.66E-16 ID:SGTC_869|Compound:0564-0080|FD-Score:4.22|P-value:1.09E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:5.32|P-value:1.60E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:8.88|P-value:3.52E-15 ID:SGTC_994|Compound:3910-0338|FD-Score:7.09|P-value:2.59E-10 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.28|P-value:9.03E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.84|P-value:3.88E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.99|P-value:2.37E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.70|P-value:1.93E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.74|P-value:1.65E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.66|P-value:6.75E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.68|P-value:6.50E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.11|P-value:1.62E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.60|P-value:8.13E-4 ID:SGTC_1752|Compound:st044819|FD-Score:4.01|P-value:2.27E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.48|P-value:2.75E-11 ID:SGTC_1761|Compound:st044296|FD-Score:4.37|P-value:6.54E-5 ID:SGTC_1762|Compound:st045503|FD-Score:11.80|P-value:1.85E-25 ID:SGTC_1774|Compound:st048371|FD-Score:4.11|P-value:1.62E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.31|P-value:8.15E-5 ID:SGTC_1954|Compound:st077032|FD-Score:3.61|P-value:7.80E-4 ID:SGTC_2090|Compound:5301565|FD-Score:5.69|P-value:3.10E-7 ID:SGTC_2268|Compound:7933382|FD-Score:4.71|P-value:1.88E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-4.71|P-value:1.77E-5 ID:SGTC_2346|Compound:9031566|FD-Score:4.54|P-value:3.56E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.72|P-value:1.82E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.12|P-value:1.53E-4 ID:SGTC_2670|Compound:beta-carotene|FD-Score:-3.67|P-value:6.23E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.09|P-value:1.74E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-4.10|P-value:1.59E-4 ID:SGTC_2939|Compound:9044578|FD-Score:3.56|P-value:9.23E-4 ID:SGTC_2970|Compound:9090938|FD-Score:-4.32|P-value:7.34E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.62|P-value:2.47E-5 ID:SGTC_3091|Compound:9116372|FD-Score:-3.58|P-value:8.23E-4 ID:SGTC_3250|Compound:9135583|FD-Score:-3.71|P-value:5.53E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-4.87|P-value:9.52E-6 ID:SGTC_3285|Compound:9112818|FD-Score:5.09|P-value:4.18E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.85|P-value:1.07E-5 ID:SGTC_3350|Compound:9150499|FD-Score:5.64|P-value:3.94E-7 ID:SGTC_3351|Compound:9150312|FD-Score:4.53|P-value:3.63E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:6.12|P-value:4.08E-8 ID:SGTC_114|Compound:0364-0001|FD-Score:3.67|P-value:6.51E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:5.06|P-value:4.78E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:7.56|P-value:1.78E-11 ID:SGTC_138|Compound:0850-0259|FD-Score:10.60|P-value:8.02E-21 ID:SGTC_197|Compound:4544-0043|FD-Score:3.83|P-value:3.98E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.78|P-value:4.63E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.89|P-value:3.32E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.63|P-value:7.35E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.64|P-value:7.28E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.28|P-value:1.91E-6 ID:SGTC_446|Compound:go6976|FD-Score:6.63|P-value:3.14E-9 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.85|P-value:1.07E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.19|P-value:2.77E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:7.25|P-value:1.04E-10 ID:SGTC_521|Compound:r062-0005|FD-Score:3.71|P-value:5.80E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.21|P-value:1.15E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:4.16|P-value:1.37E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:4.85|P-value:1.11E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:7.07|P-value:2.94E-10 ID:SGTC_612|Compound:0302-0167|FD-Score:4.69|P-value:2.05E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:4.00|P-value:2.35E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:5.12|P-value:3.67E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:10.40|P-value:3.67E-20 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.26|P-value:9.65E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:7.52|P-value:2.26E-11 ID:SGTC_829|Compound:1635-0120|FD-Score:4.29|P-value:8.61E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:9.08|P-value:8.66E-16 ID:SGTC_869|Compound:0564-0080|FD-Score:4.22|P-value:1.09E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:5.32|P-value:1.60E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:8.88|P-value:3.52E-15 ID:SGTC_994|Compound:3910-0338|FD-Score:7.09|P-value:2.59E-10 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.28|P-value:9.03E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.84|P-value:3.88E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.99|P-value:2.37E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.70|P-value:1.93E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.74|P-value:1.65E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.66|P-value:6.75E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.68|P-value:6.50E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.11|P-value:1.62E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.60|P-value:8.13E-4 ID:SGTC_1752|Compound:st044819|FD-Score:4.01|P-value:2.27E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.48|P-value:2.75E-11 ID:SGTC_1761|Compound:st044296|FD-Score:4.37|P-value:6.54E-5 ID:SGTC_1762|Compound:st045503|FD-Score:11.80|P-value:1.85E-25 ID:SGTC_1774|Compound:st048371|FD-Score:4.11|P-value:1.62E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.31|P-value:8.15E-5 ID:SGTC_1954|Compound:st077032|FD-Score:3.61|P-value:7.80E-4 ID:SGTC_2090|Compound:5301565|FD-Score:5.69|P-value:3.10E-7 ID:SGTC_2268|Compound:7933382|FD-Score:4.71|P-value:1.88E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-4.71|P-value:1.77E-5 ID:SGTC_2346|Compound:9031566|FD-Score:4.54|P-value:3.56E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.72|P-value:1.82E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.12|P-value:1.53E-4 ID:SGTC_2670|Compound:beta-carotene|FD-Score:-3.67|P-value:6.23E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.09|P-value:1.74E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-4.10|P-value:1.59E-4 ID:SGTC_2939|Compound:9044578|FD-Score:3.56|P-value:9.23E-4 ID:SGTC_2970|Compound:9090938|FD-Score:-4.32|P-value:7.34E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.62|P-value:2.47E-5 ID:SGTC_3091|Compound:9116372|FD-Score:-3.58|P-value:8.23E-4 ID:SGTC_3250|Compound:9135583|FD-Score:-3.71|P-value:5.53E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-4.87|P-value:9.52E-6 ID:SGTC_3285|Compound:9112818|FD-Score:5.09|P-value:4.18E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.85|P-value:1.07E-5 ID:SGTC_3350|Compound:9150499|FD-Score:5.64|P-value:3.94E-7 ID:SGTC_3351|Compound:9150312|FD-Score:4.53|P-value:3.63E-5

Top fitness defect scores for YOR258W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1762 st045503 11.80 25.5 µM 1.85E-25 1 1.50E-32
2 SGTC_138 0850-0259 10.60 40.9 µM 8.02E-21 cell wall 39 1.56E-26
3 SGTC_666 0272-0154 10.40 136.0 µM 3.67E-20 cell wall 6 1.12E-25
4 SGTC_863 0466-0191 9.08 129.0 µM 8.66E-16 cell wall 14 5.27E-20
5 SGTC_951 1171-0579 8.88 82.7 µM 3.52E-15 cell wall 11 3.25E-19
6 SGTC_137 0438-0332 7.56 85.4 µM 1.78E-11 ubiquinone biosynthesis & proteasome 42 2.05E-14
7 SGTC_690 glyoxal 7.52 20.3 µM 2.26E-11 31 2.79E-14
8 SGTC_1754 st042065 7.48 16.1 µM 2.75E-11 NEO1-PIK1 63 3.60E-14
9 SGTC_514 4466-0038 7.25 2.6 nM 1.04E-10 cell wall 52 2.01E-13
10 SGTC_994 3910-0338 7.09 138.0 µM 2.59E-10 calcium & mitochondrial duress 9 6.58E-13
11 SGTC_586 0957-0265 7.07 8.2 µM 2.94E-10 cell wall 10 7.74E-13
12 SGTC_446 go6976 6.63 132.0 µM 3.14E-9 1 1.66E-11
13 SGTC_58 0957-0393 6.12 9.0 µM 4.08E-8 cell wall 207 4.56E-10
14 SGTC_2090 5301565 5.69 104.5 µM 3.10E-7 TSC3-RPN4 19 6.28E-9
15 SGTC_3350 9150499 5.64 3.6 µM 3.94E-7 cell wall 19 8.56E-9
16 SGTC_886 0866-0150 5.32 153.0 µM 1.60E-6 2 5.22E-8
17 SGTC_422 cantharidin 5.28 155.0 µM 1.91E-6 cell wall signaling 29 6.57E-8
18 SGTC_474 dl-dihydrosphingosine 5.19 5.0 µM 2.77E-6 14 1.06E-7
19 SGTC_665 3914-0008 5.12 307.0 µM 3.67E-6 6 1.52E-7
20 SGTC_3285 9112818 5.09 20.9 µM 4.18E-6 cell wall 17 1.80E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.364 1.70E-105 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.351 3.82E-98 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.315 4.35E-78 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.313 3.73E-77 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.307 3.09E-74 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.307 7.37E-74 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.301 3.53E-71 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.294 8.00E-68 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.291 1.25E-66 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.289 1.64E-65 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.285 1.64E-63 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.283 5.56E-63 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.282 2.06E-62 YGR056W RSC1 hom Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
0.279 3.72E-61 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.278 8.61E-61 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member