YOR259C / RPT4

One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR259C / RPT4

Click on Significant Values for Screen Details ID:SGTC_104|Compound:0074-0074|FD-Score:4.05|P-value:9.29E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:-5.87|P-value:1.20E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.86|P-value:1.25E-7 ID:SGTC_122|Compound:0236-0016|FD-Score:5.49|P-value:2.46E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:5.24|P-value:7.74E-7 ID:SGTC_124|Compound:0136-0217|FD-Score:-3.97|P-value:2.67E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.19|P-value:5.60E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.10|P-value:7.80E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.16|P-value:6.27E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.95|P-value:2.85E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.60|P-value:8.68E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.35|P-value:4.79E-7 ID:SGTC_553|Compound:k007-0256|FD-Score:7.36|P-value:1.01E-11 ID:SGTC_573|Compound:3702-0553|FD-Score:3.41|P-value:7.90E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.48|P-value:6.33E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:-4.66|P-value:2.22E-5 ID:SGTC_784|Compound:4579-0127|FD-Score:-5.50|P-value:6.58E-7 ID:SGTC_999|Compound:4022-0066|FD-Score:3.70|P-value:3.09E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.77|P-value:5.06E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.65|P-value:3.75E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.80|P-value:2.25E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.68|P-value:3.30E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.72|P-value:7.27E-6 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.78|P-value:2.41E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.83|P-value:2.02E-4 ID:SGTC_1621|Compound:st003705|FD-Score:3.75|P-value:2.64E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.63|P-value:3.90E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.37|P-value:8.86E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.05|P-value:9.46E-5 ID:SGTC_1775|Compound:st081588|FD-Score:3.54|P-value:5.30E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.50|P-value:6.01E-4 ID:SGTC_2072|Compound:5231554|FD-Score:3.61|P-value:4.23E-4 ID:SGTC_2124|Compound:5246320|FD-Score:6.03|P-value:1.78E-8 ID:SGTC_2143|Compound:5623677|FD-Score:-3.87|P-value:3.74E-4 ID:SGTC_2148|Compound:5546263|FD-Score:-3.88|P-value:3.62E-4 ID:SGTC_2294|Compound:7971431|FD-Score:4.95|P-value:2.79E-6 ID:SGTC_2308|Compound:7584454|FD-Score:3.59|P-value:4.53E-4 ID:SGTC_2326|Compound:6164455|FD-Score:4.04|P-value:9.90E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.78|P-value:2.38E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.73|P-value:6.98E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.01|P-value:2.33E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-3.66|P-value:7.36E-4 ID:SGTC_2795|Compound:7780010|FD-Score:6.36|P-value:3.25E-9 ID:SGTC_2832|Compound:7992762|FD-Score:3.49|P-value:6.26E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.62|P-value:4.13E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.59|P-value:4.56E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.00|P-value:1.14E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.89|P-value:3.46E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:4.05|P-value:9.29E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:-5.87|P-value:1.20E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.86|P-value:1.25E-7 ID:SGTC_122|Compound:0236-0016|FD-Score:5.49|P-value:2.46E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:5.24|P-value:7.74E-7 ID:SGTC_124|Compound:0136-0217|FD-Score:-3.97|P-value:2.67E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.19|P-value:5.60E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.10|P-value:7.80E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.16|P-value:6.27E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.95|P-value:2.85E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.60|P-value:8.68E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.35|P-value:4.79E-7 ID:SGTC_553|Compound:k007-0256|FD-Score:7.36|P-value:1.01E-11 ID:SGTC_573|Compound:3702-0553|FD-Score:3.41|P-value:7.90E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.48|P-value:6.33E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:-4.66|P-value:2.22E-5 ID:SGTC_784|Compound:4579-0127|FD-Score:-5.50|P-value:6.58E-7 ID:SGTC_999|Compound:4022-0066|FD-Score:3.70|P-value:3.09E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.77|P-value:5.06E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.65|P-value:3.75E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.80|P-value:2.25E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.68|P-value:3.30E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.72|P-value:7.27E-6 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.78|P-value:2.41E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.83|P-value:2.02E-4 ID:SGTC_1621|Compound:st003705|FD-Score:3.75|P-value:2.64E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.63|P-value:3.90E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.37|P-value:8.86E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.05|P-value:9.46E-5 ID:SGTC_1775|Compound:st081588|FD-Score:3.54|P-value:5.30E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.50|P-value:6.01E-4 ID:SGTC_2072|Compound:5231554|FD-Score:3.61|P-value:4.23E-4 ID:SGTC_2124|Compound:5246320|FD-Score:6.03|P-value:1.78E-8 ID:SGTC_2143|Compound:5623677|FD-Score:-3.87|P-value:3.74E-4 ID:SGTC_2148|Compound:5546263|FD-Score:-3.88|P-value:3.62E-4 ID:SGTC_2294|Compound:7971431|FD-Score:4.95|P-value:2.79E-6 ID:SGTC_2308|Compound:7584454|FD-Score:3.59|P-value:4.53E-4 ID:SGTC_2326|Compound:6164455|FD-Score:4.04|P-value:9.90E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.78|P-value:2.38E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.73|P-value:6.98E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.01|P-value:2.33E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-3.66|P-value:7.36E-4 ID:SGTC_2795|Compound:7780010|FD-Score:6.36|P-value:3.25E-9 ID:SGTC_2832|Compound:7992762|FD-Score:3.49|P-value:6.26E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.62|P-value:4.13E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.59|P-value:4.56E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.00|P-value:1.14E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.89|P-value:3.46E-4

Top fitness defect scores for YOR259C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_553 k007-0256 7.36 45.7 µM 1.01E-11 heme biosynthesis & mitochondrial translocase 6 9.10E-14 1.02
2 SGTC_2795 7780010 6.36 54.5 µM 3.25E-9 1 1.03E-10 1.19
3 SGTC_2124 5246320 6.03 200.0 µM 1.78E-8 heme biosynthesis & mitochondrial translocase 14 8.13E-10 0.12
4 SGTC_122 0236-0016 5.49 402.4 µM 2.46E-7 RNA processing & uracil transport 3 1.96E-8 0.11
5 SGTC_550 4123-0385 5.35 25.7 µM 4.79E-7 TSC3-RPN4 3 4.40E-8 0.80
6 SGTC_123 0083-0120 5.24 66.7 µM 7.74E-7 6 7.87E-8 0.12
7 SGTC_2294 7971431 4.95 200.0 µM 2.79E-6 1 3.71E-7 0.67
8 SGTC_2563 tomatine 4.73 8.5 µM 6.98E-6 9 1.12E-6 0.08
9 SGTC_1564 D-Arginine 4.72 115.0 µM 7.27E-6 3 1.18E-6 0.01
10 SGTC_148 3253-0744 4.19 288.0 µM 5.60E-5 6 1.37E-5 0.03
11 SGTC_339 3091-4833 4.16 71.4 µM 6.27E-5 iron homeostasis 8 1.57E-5 0.02
12 SGTC_236 1509-0011 4.10 5.3 µM 7.80E-5 25 2.04E-5 0.12
13 SGTC_104 0074-0074 4.05 591.0 µM 9.29E-5 14 2.52E-5 0.13
14 SGTC_1765 st044815 4.05 90.4 µM 9.46E-5 10 2.57E-5 0.26
15 SGTC_2326 6164455 4.04 177.6 µM 9.90E-5 mitochondrial processes 13 2.71E-5 0.11
16 SGTC_3142 9086498 4.00 49.5 µM 1.14E-4 iron homeostasis 44 3.21E-5 0.13
17 SGTC_1608 st001473 3.83 40.5 µM 2.02E-4 7 6.38E-5 0.25
18 SGTC_1455 4262-0120 3.80 131.0 µM 2.25E-4 plasma membrane duress 5 7.24E-5 0.04
19 SGTC_2409 alverine citrate 3.78 64.0 µM 2.38E-4 16 7.75E-5 0.10
20 SGTC_1575 cyproterone acetate 3.78 48.0 µM 2.41E-4 7 7.87E-5 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.326 8.70E-84 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.202 4.17E-32 YPR108W RPN7 het Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
0.201 5.47E-32 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.199 2.01E-31 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.199 3.57E-31 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.173 4.32E-24 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.141 2.71E-16 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.132 1.75E-14 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.128 9.53E-14 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.126 2.38E-13 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.126 2.67E-13 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.125 4.34E-13 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.115 2.26E-11 YOR227W HER1 hom Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
0.108 3.35E-10 YBL021C HAP3 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
0.105 1.11E-9 YOL052C-A DDR2 hom Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication