YOR260W / GCD1

Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR260W / GCD1

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:5.61|P-value:1.59E-7 ID:SGTC_108|Compound:0509-0257|FD-Score:3.42|P-value:8.97E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.47|P-value:3.45E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.52|P-value:7.09E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:5.63|P-value:1.47E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:3.57|P-value:5.65E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.91|P-value:1.83E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:11.50|P-value:5.30E-26 ID:SGTC_539|Compound:4859-0462|FD-Score:6.86|P-value:2.04E-10 ID:SGTC_540|Compound:1187-0821|FD-Score:3.69|P-value:3.82E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.46|P-value:2.36E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:9.99|P-value:4.49E-20 ID:SGTC_686|Compound:1319-0111|FD-Score:4.38|P-value:3.25E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:5.64|P-value:1.39E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.43|P-value:9.43E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:6.65|P-value:6.71E-10 ID:SGTC_969|Compound:1313-0238|FD-Score:3.67|P-value:4.04E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:-3.64|P-value:4.78E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.20|P-value:1.06E-6 ID:SGTC_1494|Compound:4358-1428|FD-Score:-4.56|P-value:1.77E-5 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.79|P-value:2.75E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.57|P-value:5.56E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.66|P-value:4.19E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.91|P-value:3.77E-6 ID:SGTC_1872|Compound:amphotericin b|FD-Score:7.39|P-value:8.46E-12 ID:SGTC_1891|Compound:st059073|FD-Score:4.04|P-value:1.14E-4 ID:SGTC_2067|Compound:5224629|FD-Score:3.50|P-value:6.98E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.96|P-value:3.05E-6 ID:SGTC_2104|Compound:5389443|FD-Score:3.82|P-value:2.47E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.55|P-value:6.04E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.79|P-value:2.71E-4 ID:SGTC_2192|Compound:5915882|FD-Score:4.54|P-value:1.77E-5 ID:SGTC_2335|Compound:9018011|FD-Score:4.35|P-value:3.67E-5 ID:SGTC_2374|Compound:9071832|FD-Score:3.40|P-value:9.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.36|P-value:3.51E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-5.27|P-value:8.63E-7 ID:SGTC_2411|Compound:st077133|FD-Score:20.70|P-value:1.46E-79 ID:SGTC_2415|Compound:st077333|FD-Score:3.47|P-value:7.65E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.88|P-value:2.15E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.78|P-value:6.50E-6 ID:SGTC_2460|Compound:5552655|FD-Score:3.41|P-value:9.43E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:5.98|P-value:2.57E-8 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.58|P-value:1.86E-7 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:4.82|P-value:5.65E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.41|P-value:9.83E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.77|P-value:7.37E-6 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.92|P-value:1.85E-4 ID:SGTC_2601|Compound:securinine|FD-Score:5.77|P-value:7.38E-8 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.90|P-value:1.60E-10 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.69|P-value:1.10E-7 ID:SGTC_2788|Compound:5282734|FD-Score:4.12|P-value:8.48E-5 ID:SGTC_2808|Compound:7946468|FD-Score:3.66|P-value:4.29E-4 ID:SGTC_2839|Compound:9003951|FD-Score:-3.54|P-value:6.50E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.91|P-value:3.54E-8 ID:SGTC_2884|Compound:9058502|FD-Score:4.73|P-value:8.08E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.93|P-value:1.78E-4 ID:SGTC_2932|Compound:9016711|FD-Score:5.76|P-value:7.77E-8 ID:SGTC_2952|Compound:9073731|FD-Score:5.88|P-value:4.28E-8 ID:SGTC_3038|Compound:9091290|FD-Score:3.39|P-value:9.78E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.57|P-value:5.66E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.94|P-value:1.64E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.97|P-value:1.60E-4 ID:SGTC_3152|Compound:9099253|FD-Score:7.31|P-value:1.42E-11 ID:SGTC_3155|Compound:9099265|FD-Score:-3.43|P-value:9.38E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.72|P-value:3.42E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.54|P-value:6.56E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:5.61|P-value:1.59E-7 ID:SGTC_108|Compound:0509-0257|FD-Score:3.42|P-value:8.97E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.47|P-value:3.45E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.52|P-value:7.09E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:5.63|P-value:1.47E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:3.57|P-value:5.65E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.91|P-value:1.83E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:11.50|P-value:5.30E-26 ID:SGTC_539|Compound:4859-0462|FD-Score:6.86|P-value:2.04E-10 ID:SGTC_540|Compound:1187-0821|FD-Score:3.69|P-value:3.82E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.46|P-value:2.36E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:9.99|P-value:4.49E-20 ID:SGTC_686|Compound:1319-0111|FD-Score:4.38|P-value:3.25E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:5.64|P-value:1.39E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.43|P-value:9.43E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:6.65|P-value:6.71E-10 ID:SGTC_969|Compound:1313-0238|FD-Score:3.67|P-value:4.04E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:-3.64|P-value:4.78E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.20|P-value:1.06E-6 ID:SGTC_1494|Compound:4358-1428|FD-Score:-4.56|P-value:1.77E-5 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.79|P-value:2.75E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.57|P-value:5.56E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.66|P-value:4.19E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.91|P-value:3.77E-6 ID:SGTC_1872|Compound:amphotericin b|FD-Score:7.39|P-value:8.46E-12 ID:SGTC_1891|Compound:st059073|FD-Score:4.04|P-value:1.14E-4 ID:SGTC_2067|Compound:5224629|FD-Score:3.50|P-value:6.98E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.96|P-value:3.05E-6 ID:SGTC_2104|Compound:5389443|FD-Score:3.82|P-value:2.47E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.55|P-value:6.04E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.79|P-value:2.71E-4 ID:SGTC_2192|Compound:5915882|FD-Score:4.54|P-value:1.77E-5 ID:SGTC_2335|Compound:9018011|FD-Score:4.35|P-value:3.67E-5 ID:SGTC_2374|Compound:9071832|FD-Score:3.40|P-value:9.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.36|P-value:3.51E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-5.27|P-value:8.63E-7 ID:SGTC_2411|Compound:st077133|FD-Score:20.70|P-value:1.46E-79 ID:SGTC_2415|Compound:st077333|FD-Score:3.47|P-value:7.65E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.88|P-value:2.15E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.78|P-value:6.50E-6 ID:SGTC_2460|Compound:5552655|FD-Score:3.41|P-value:9.43E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:5.98|P-value:2.57E-8 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.58|P-value:1.86E-7 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:4.82|P-value:5.65E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.41|P-value:9.83E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.77|P-value:7.37E-6 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.92|P-value:1.85E-4 ID:SGTC_2601|Compound:securinine|FD-Score:5.77|P-value:7.38E-8 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.90|P-value:1.60E-10 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.69|P-value:1.10E-7 ID:SGTC_2788|Compound:5282734|FD-Score:4.12|P-value:8.48E-5 ID:SGTC_2808|Compound:7946468|FD-Score:3.66|P-value:4.29E-4 ID:SGTC_2839|Compound:9003951|FD-Score:-3.54|P-value:6.50E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.91|P-value:3.54E-8 ID:SGTC_2884|Compound:9058502|FD-Score:4.73|P-value:8.08E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.93|P-value:1.78E-4 ID:SGTC_2932|Compound:9016711|FD-Score:5.76|P-value:7.77E-8 ID:SGTC_2952|Compound:9073731|FD-Score:5.88|P-value:4.28E-8 ID:SGTC_3038|Compound:9091290|FD-Score:3.39|P-value:9.78E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.57|P-value:5.66E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.94|P-value:1.64E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.97|P-value:1.60E-4 ID:SGTC_3152|Compound:9099253|FD-Score:7.31|P-value:1.42E-11 ID:SGTC_3155|Compound:9099265|FD-Score:-3.43|P-value:9.38E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.72|P-value:3.42E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.54|P-value:6.56E-4

Top fitness defect scores for YOR260W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2411 st077133 20.70 39.9 µM 1.46E-79 RNA pol III & RNase P/MRP 4 1.33E-95 4.13
2 SGTC_392 N-ethylmaleimide 11.50 8.3 µM 5.30E-26 RNA pol III & RNase P/MRP 3 5.74E-31 5.03
3 SGTC_682 0kpi-0099 9.99 17.0 µM 4.49E-20 2 8.03E-24 0.81
4 SGTC_1872 amphotericin b 7.39 148.0 nM 8.46E-12 RNA pol III & RNase P/MRP 10 7.38E-14 0.41
5 SGTC_3152 9099253 7.31 49.5 µM 1.42E-11 NEO1-PIK1 9 1.38E-13 0.20
6 SGTC_2612 dictamnine 6.90 15.5 µM 1.60E-10 RNA pol III & RNase P/MRP 16 2.52E-12 0.70
7 SGTC_539 4859-0462 6.86 2.5 µM 2.04E-10 RNA pol III & RNase P/MRP 9 3.39E-12 1.19
8 SGTC_861 0443-0273 6.65 110.0 µM 6.71E-10 1 1.42E-11 1.44
9 SGTC_2510 benzyl isothiocyanate 5.98 8.3 µM 2.57E-8 8 1.13E-9 0.40
10 SGTC_2841 9004761 5.91 39.0 µM 3.54E-8 amide catabolism 2 1.66E-9 0.66
11 SGTC_2952 9073731 5.88 13.8 µM 4.28E-8 tubulin folding & SWR complex 4 2.09E-9 0.18
12 SGTC_2601 securinine 5.77 100.0 µM 7.38E-8 5 4.01E-9 0.56
13 SGTC_2932 9016711 5.76 44.5 µM 7.77E-8 2 4.27E-9 0.48
14 SGTC_2651 1327-0036 5.69 5.0 µM 1.10E-7 35 6.45E-9 0.28
15 SGTC_852 0418-0143 5.64 439.0 µM 1.39E-7 calcium & mitochondrial duress 4 8.56E-9 0.56
16 SGTC_152 4469-0003 5.63 551.2 µM 1.47E-7 4 9.15E-9 0.16
17 SGTC_87 0100-0052 5.61 35.5 µM 1.59E-7 8 1.00E-8 0.01
18 SGTC_2522 biochanin a 5.58 4.9 µM 1.86E-7 RNA pol III & RNase P/MRP 5 1.21E-8 0.02
19 SGTC_1369 1866-0067 5.20 208.0 µM 1.06E-6 RPP1 & pyrimidine depletion 5 9.81E-8 0.36
20 SGTC_2090 5301565 4.96 104.5 µM 3.05E-6 TSC3-RPN4 3 3.48E-7 0.54

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.411 5.37E-137 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.406 5.17E-133 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.365 2.93E-106 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.268 2.75E-56 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.241 2.14E-45 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.197 1.45E-30 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.192 2.87E-29 YBR167C POP7 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.176 1.25E-24 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.171 1.62E-23 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.167 2.06E-22 YGR090W UTP22 het Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
0.164 1.11E-21 YIL164C NIT1 hom Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
0.162 4.33E-21 YDL150W RPC53 het RNA polymerase III subunit C53
0.151 1.30E-18 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.143 8.31E-17 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.141 2.62E-16 YPR190C RPC82 het RNA polymerase III subunit C82