YOR261C / RPN8

Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR261C / RPN8

Click on Significant Values for Screen Details ID:SGTC_143|Compound:4032-1194|FD-Score:-3.51|P-value:5.53E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.06|P-value:3.78E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:2.87|P-value:7.97E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.07|P-value:3.72E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.80|P-value:1.44E-5 ID:SGTC_462|Compound:ag-879|FD-Score:2.94|P-value:6.20E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:2.97|P-value:5.49E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.49|P-value:6.02E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:4.06|P-value:4.15E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.97|P-value:6.32E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.25|P-value:1.71E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-2.88|P-value:7.58E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.19|P-value:2.10E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.35|P-value:8.29E-7 ID:SGTC_912|Compound:3474-0017|FD-Score:4.15|P-value:2.59E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:3.01|P-value:4.67E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.15|P-value:2.70E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.32|P-value:1.32E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.28|P-value:1.55E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-2.86|P-value:8.16E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.13|P-value:2.79E-6 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.02|P-value:4.56E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.95|P-value:5.97E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.85|P-value:8.82E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:2.95|P-value:5.89E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.03|P-value:4.16E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-2.89|P-value:7.42E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:2.99|P-value:5.16E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:4.16|P-value:2.45E-6 ID:SGTC_1518|Compound:st038567|FD-Score:-3.85|P-value:1.14E-5 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.07|P-value:3.56E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:-3.95|P-value:6.80E-6 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:2.85|P-value:8.69E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.58|P-value:4.23E-5 ID:SGTC_1725|Compound:st036284|FD-Score:5.47|P-value:9.22E-10 ID:SGTC_1729|Compound:st035615|FD-Score:5.07|P-value:1.25E-8 ID:SGTC_1745|Compound:st081495|FD-Score:-3.40|P-value:8.86E-5 ID:SGTC_1751|Compound:st044839|FD-Score:2.83|P-value:9.49E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.52|P-value:5.21E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.23|P-value:1.56E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.69|P-value:2.43E-5 ID:SGTC_1797|Compound:st048695|FD-Score:3.79|P-value:1.59E-5 ID:SGTC_1871|Compound:st057448|FD-Score:3.36|P-value:1.11E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.95|P-value:7.04E-6 ID:SGTC_2085|Compound:5307070|FD-Score:2.82|P-value:9.94E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.14|P-value:2.79E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:5.63|P-value:3.02E-10 ID:SGTC_2322|Compound:7413319|FD-Score:-4.46|P-value:4.51E-7 ID:SGTC_2342|Compound:7990637|FD-Score:-3.91|P-value:8.31E-6 ID:SGTC_2348|Compound:9022543|FD-Score:3.92|P-value:8.20E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.19|P-value:2.24E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.77|P-value:1.68E-5 ID:SGTC_2440|Compound:5628896|FD-Score:-2.93|P-value:6.23E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.87|P-value:8.17E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-4.26|P-value:1.40E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.44|P-value:5.03E-7 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.31|P-value:1.39E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.21|P-value:2.05E-4 ID:SGTC_2702|Compound:st077768|FD-Score:4.11|P-value:3.10E-6 ID:SGTC_2704|Compound:st078022|FD-Score:-3.21|P-value:2.01E-4 ID:SGTC_2707|Compound:st078556|FD-Score:5.50|P-value:7.58E-10 ID:SGTC_2716|Compound:st080858|FD-Score:-2.91|P-value:6.71E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-2.97|P-value:5.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-2.91|P-value:6.79E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-3.35|P-value:1.13E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.08|P-value:3.55E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.06|P-value:3.77E-4 ID:SGTC_3101|Compound:9120531|FD-Score:2.84|P-value:9.16E-4 ID:SGTC_3107|Compound:9121808|FD-Score:2.97|P-value:5.62E-4 ID:SGTC_3142|Compound:9086498|FD-Score:2.97|P-value:5.47E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.39|P-value:9.91E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.15|P-value:2.61E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-3.25|P-value:1.72E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.51|P-value:5.53E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.06|P-value:3.78E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:2.87|P-value:7.97E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.07|P-value:3.72E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.80|P-value:1.44E-5 ID:SGTC_462|Compound:ag-879|FD-Score:2.94|P-value:6.20E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:2.97|P-value:5.49E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.49|P-value:6.02E-5 ID:SGTC_634|Compound:1611-4317|FD-Score:4.06|P-value:4.15E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.97|P-value:6.32E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.25|P-value:1.71E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-2.88|P-value:7.58E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.19|P-value:2.10E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-4.35|P-value:8.29E-7 ID:SGTC_912|Compound:3474-0017|FD-Score:4.15|P-value:2.59E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:3.01|P-value:4.67E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.15|P-value:2.70E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.32|P-value:1.32E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.28|P-value:1.55E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-2.86|P-value:8.16E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.13|P-value:2.79E-6 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.02|P-value:4.56E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.95|P-value:5.97E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:2.85|P-value:8.82E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:2.95|P-value:5.89E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.03|P-value:4.16E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-2.89|P-value:7.42E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:2.99|P-value:5.16E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:4.16|P-value:2.45E-6 ID:SGTC_1518|Compound:st038567|FD-Score:-3.85|P-value:1.14E-5 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.07|P-value:3.56E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:-3.95|P-value:6.80E-6 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:2.85|P-value:8.69E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.58|P-value:4.23E-5 ID:SGTC_1725|Compound:st036284|FD-Score:5.47|P-value:9.22E-10 ID:SGTC_1729|Compound:st035615|FD-Score:5.07|P-value:1.25E-8 ID:SGTC_1745|Compound:st081495|FD-Score:-3.40|P-value:8.86E-5 ID:SGTC_1751|Compound:st044839|FD-Score:2.83|P-value:9.49E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.52|P-value:5.21E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.23|P-value:1.56E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.69|P-value:2.43E-5 ID:SGTC_1797|Compound:st048695|FD-Score:3.79|P-value:1.59E-5 ID:SGTC_1871|Compound:st057448|FD-Score:3.36|P-value:1.11E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.95|P-value:7.04E-6 ID:SGTC_2085|Compound:5307070|FD-Score:2.82|P-value:9.94E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.14|P-value:2.79E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:5.63|P-value:3.02E-10 ID:SGTC_2322|Compound:7413319|FD-Score:-4.46|P-value:4.51E-7 ID:SGTC_2342|Compound:7990637|FD-Score:-3.91|P-value:8.31E-6 ID:SGTC_2348|Compound:9022543|FD-Score:3.92|P-value:8.20E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.19|P-value:2.24E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.77|P-value:1.68E-5 ID:SGTC_2440|Compound:5628896|FD-Score:-2.93|P-value:6.23E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.87|P-value:8.17E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-4.26|P-value:1.40E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.44|P-value:5.03E-7 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.31|P-value:1.39E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.21|P-value:2.05E-4 ID:SGTC_2702|Compound:st077768|FD-Score:4.11|P-value:3.10E-6 ID:SGTC_2704|Compound:st078022|FD-Score:-3.21|P-value:2.01E-4 ID:SGTC_2707|Compound:st078556|FD-Score:5.50|P-value:7.58E-10 ID:SGTC_2716|Compound:st080858|FD-Score:-2.91|P-value:6.71E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-2.97|P-value:5.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-2.91|P-value:6.79E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-3.35|P-value:1.13E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.08|P-value:3.55E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.06|P-value:3.77E-4 ID:SGTC_3101|Compound:9120531|FD-Score:2.84|P-value:9.16E-4 ID:SGTC_3107|Compound:9121808|FD-Score:2.97|P-value:5.62E-4 ID:SGTC_3142|Compound:9086498|FD-Score:2.97|P-value:5.47E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.39|P-value:9.91E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.15|P-value:2.61E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-3.25|P-value:1.72E-4

Top fitness defect scores for YOR261C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 5.63 49.5 µM 3.02E-10 6 8.95E-9 0.11
2 SGTC_2707 st078556 5.50 66.6 µM 7.58E-10 1 1.92E-8 0.92
3 SGTC_1725 st036284 5.47 20.6 µM 9.22E-10 1 2.26E-8 0.91
4 SGTC_1729 st035615 5.07 46.0 µM 1.25E-8 5 1.97E-7 0.82
5 SGTC_819 1013-0258 4.19 27.7 µM 2.10E-6 3 1.41E-5 0.01
6 SGTC_1507 4075-3230 4.16 155.0 µM 2.45E-6 1 1.60E-5 0.45
7 SGTC_912 3474-0017 4.15 2.2 µM 2.59E-6 1 1.68E-5 0.26
8 SGTC_2702 st077768 4.11 96.8 µM 3.10E-6 6 1.95E-5 0.06
9 SGTC_634 1611-4317 4.06 11.2 µM 4.15E-6 ERG2 8 2.49E-5 0.01
10 SGTC_2004 4023728 3.95 41.9 µM 7.04E-6 26 3.87E-5 0.04
11 SGTC_2348 9022543 3.92 33.4 µM 8.20E-6 mitochondrial processes 32 4.40E-5 0.01
12 SGTC_1797 st048695 3.79 56.8 µM 1.59E-5 NEO1 17 7.66E-5 0.16
13 SGTC_2415 st077333 3.77 57.7 µM 1.68E-5 18 8.01E-5 0.13
14 SGTC_1556 plumbagin 3.58 1.5 µM 4.23E-5 superoxide 22 1.73E-4 0.00
15 SGTC_3215 9129942 3.39 49.5 µM 9.91E-5 8 3.53E-4 0.06
16 SGTC_1871 st057448 3.36 67.3 µM 1.11E-4 15 3.89E-4 0.07
17 SGTC_1082 camptothecin 3.32 424.9 nM 1.32E-4 DNA damage response 29 4.48E-4 0.12
18 SGTC_2664 lyso-paf C-16 3.31 73.5 µM 1.39E-4 7 4.69E-4 0.03
19 SGTC_1113 artemisinin 3.28 1.2 µM 1.55E-4 heme biosynthesis & mitochondrial translocase 42 5.13E-4 0.13
20 SGTC_2405 ticlatone 3.19 3.5 µM 2.24E-4 30 6.99E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.178 2.88E-25 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.143 8.61E-17 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.141 2.66E-16 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.137 1.30E-15 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.130 4.38E-14 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.118 7.17E-12 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.116 1.74E-11 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.115 2.18E-11 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.113 6.20E-11 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.102 3.09E-9 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.094 5.24E-8 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.093 6.21E-8 YNL112W DBP2 het ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.093 6.77E-8 YKL007W CAP1 hom Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
0.092 9.97E-8 YJL084C ALY2 hom Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication
0.088 3.49E-7 YLR250W SSP120 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern