YOR317W / FAA1

Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR317W / FAA1

Click on Significant Values for Screen Details ID:SGTC_26|Compound:0302-0163|FD-Score:3.25|P-value:6.53E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.34|P-value:4.78E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.13|P-value:2.26E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-6.76|P-value:1.22E-11 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.15|P-value:9.40E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.27|P-value:1.19E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.89|P-value:6.02E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:3.75|P-value:1.03E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.84|P-value:7.66E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.20|P-value:1.65E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:4.65|P-value:2.16E-6 ID:SGTC_725|Compound:1497-0963|FD-Score:3.37|P-value:4.27E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:4.94|P-value:5.04E-7 ID:SGTC_736|Compound:0958-0254|FD-Score:5.26|P-value:9.75E-8 ID:SGTC_764|Compound:1951-2361|FD-Score:3.34|P-value:4.78E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:4.09|P-value:2.56E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:3.28|P-value:5.94E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.79|P-value:1.08E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.28|P-value:5.97E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.19|P-value:8.06E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.14|P-value:9.78E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-4.34|P-value:9.22E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.47|P-value:3.04E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.55|P-value:2.25E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.78|P-value:9.22E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:-4.15|P-value:2.13E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.26|P-value:6.32E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.49|P-value:2.77E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.94|P-value:4.83E-5 ID:SGTC_2047|Compound:4141938|FD-Score:4.37|P-value:7.56E-6 ID:SGTC_2213|Compound:7173844|FD-Score:3.75|P-value:1.04E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.34|P-value:4.69E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.50|P-value:2.75E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.16|P-value:9.11E-4 ID:SGTC_2851|Compound:9015922|FD-Score:6.12|P-value:7.13E-10 ID:SGTC_2855|Compound:9016525|FD-Score:3.64|P-value:1.56E-4 ID:SGTC_2880|Compound:9042983|FD-Score:-3.38|P-value:4.21E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.10|P-value:2.54E-5 ID:SGTC_2931|Compound:9014674|FD-Score:-3.90|P-value:5.94E-5 ID:SGTC_2998|Compound:9066762|FD-Score:4.16|P-value:1.94E-5 ID:SGTC_3144|Compound:9087057|FD-Score:4.20|P-value:1.66E-5 ID:SGTC_3195|Compound:9113082|FD-Score:3.97|P-value:4.28E-5 ID:SGTC_3226|Compound:9131256|FD-Score:3.36|P-value:4.46E-4 ID:SGTC_3244|Compound:9134489|FD-Score:4.13|P-value:2.20E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-4.00|P-value:3.98E-5 ID:SGTC_3252|Compound:9135871|FD-Score:-4.26|P-value:1.29E-5 ID:SGTC_3258|Compound:9138477|FD-Score:-3.65|P-value:1.57E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.96|P-value:4.55E-5 ID:SGTC_3270|Compound:9137563|FD-Score:-3.14|P-value:9.61E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.29|P-value:5.67E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:3.25|P-value:6.53E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.34|P-value:4.78E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.13|P-value:2.26E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-6.76|P-value:1.22E-11 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.15|P-value:9.40E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.27|P-value:1.19E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.89|P-value:6.02E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:3.75|P-value:1.03E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.84|P-value:7.66E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.20|P-value:1.65E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:4.65|P-value:2.16E-6 ID:SGTC_725|Compound:1497-0963|FD-Score:3.37|P-value:4.27E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:4.94|P-value:5.04E-7 ID:SGTC_736|Compound:0958-0254|FD-Score:5.26|P-value:9.75E-8 ID:SGTC_764|Compound:1951-2361|FD-Score:3.34|P-value:4.78E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:4.09|P-value:2.56E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:3.28|P-value:5.94E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.79|P-value:1.08E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.28|P-value:5.97E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.19|P-value:8.06E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.14|P-value:9.78E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-4.34|P-value:9.22E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.47|P-value:3.04E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.55|P-value:2.25E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.78|P-value:9.22E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:-4.15|P-value:2.13E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.26|P-value:6.32E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.49|P-value:2.77E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.94|P-value:4.83E-5 ID:SGTC_2047|Compound:4141938|FD-Score:4.37|P-value:7.56E-6 ID:SGTC_2213|Compound:7173844|FD-Score:3.75|P-value:1.04E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.34|P-value:4.69E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.50|P-value:2.75E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.16|P-value:9.11E-4 ID:SGTC_2851|Compound:9015922|FD-Score:6.12|P-value:7.13E-10 ID:SGTC_2855|Compound:9016525|FD-Score:3.64|P-value:1.56E-4 ID:SGTC_2880|Compound:9042983|FD-Score:-3.38|P-value:4.21E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.10|P-value:2.54E-5 ID:SGTC_2931|Compound:9014674|FD-Score:-3.90|P-value:5.94E-5 ID:SGTC_2998|Compound:9066762|FD-Score:4.16|P-value:1.94E-5 ID:SGTC_3144|Compound:9087057|FD-Score:4.20|P-value:1.66E-5 ID:SGTC_3195|Compound:9113082|FD-Score:3.97|P-value:4.28E-5 ID:SGTC_3226|Compound:9131256|FD-Score:3.36|P-value:4.46E-4 ID:SGTC_3244|Compound:9134489|FD-Score:4.13|P-value:2.20E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-4.00|P-value:3.98E-5 ID:SGTC_3252|Compound:9135871|FD-Score:-4.26|P-value:1.29E-5 ID:SGTC_3258|Compound:9138477|FD-Score:-3.65|P-value:1.57E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.96|P-value:4.55E-5 ID:SGTC_3270|Compound:9137563|FD-Score:-3.14|P-value:9.61E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.29|P-value:5.67E-4

Top fitness defect scores for YOR317W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2851 9015922 6.12 71.4 µM 7.13E-10 7 4.65E-10
2 SGTC_736 0958-0254 5.26 92.6 µM 9.75E-8 Golgi 7 7.11E-8
3 SGTC_735 3076-0345 4.94 636.0 µM 5.04E-7 11 3.81E-7
4 SGTC_813 k007-0158 4.79 173.0 µM 1.08E-6 fatty acid desaturase (OLE1) 19 8.32E-7
5 SGTC_513 4245-1575 4.65 41.7 µM 2.16E-6 unfolded protein response 86 1.69E-6
6 SGTC_2047 4141938 4.37 200.0 µM 7.56E-6 3 6.08E-6
7 SGTC_103 0844-0104 4.27 51.8 µM 1.19E-5 ERAD & cell cycle 130 9.70E-6
8 SGTC_3144 9087057 4.20 49.5 µM 1.66E-5 12 1.36E-5
9 SGTC_2998 9066762 4.16 71.4 µM 1.94E-5 RPP1 & pyrimidine depletion 21 1.60E-5
10 SGTC_3244 9134489 4.13 49.5 µM 2.20E-5 13 1.81E-5
11 SGTC_2896 9052656 4.10 71.4 µM 2.54E-5 endomembrane recycling 104 2.10E-5
12 SGTC_765 1319-0117 4.09 244.0 µM 2.56E-5 60S ribosome export 9 2.11E-5
13 SGTC_3195 9113082 3.97 49.5 µM 4.28E-5 18 3.57E-5
14 SGTC_3263 9137018 3.96 49.5 µM 4.55E-5 16 3.80E-5
15 SGTC_2001 4023502 3.94 57.7 µM 4.83E-5 mitochondrial processes 17 4.04E-5
16 SGTC_252 miconazole 3.89 1.3 nM 6.02E-5 ergosterol depletion effects on membrane 35 5.06E-5
17 SGTC_1405 3909-7894 3.78 12.0 µM 9.22E-5 60S ribosome export 12 7.83E-5
18 SGTC_353 1988-1175 3.75 58.3 µM 1.03E-4 unfolded protein response 30 8.74E-5
19 SGTC_2213 7173844 3.75 200.0 µM 1.04E-4 16 8.83E-5
20 SGTC_2855 9016525 3.64 58.4 µM 1.56E-4 24 1.34E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 2.32E-12 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.104 1.39E-9 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.101 4.54E-9 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.086 6.03E-7 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.085 8.38E-7 YER015W FAA2 hom Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome
0.085 8.42E-7 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.084 1.07E-6 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.084 1.13E-6 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.080 3.44E-6 YMR276W DSK2 hom Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
0.076 9.80E-6 YOL013C HRD1 hom Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
0.076 1.09E-5 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.076 1.18E-5 YHL029C_p OCA5_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.075 1.49E-5 YOR053W_d YOR053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
0.075 1.60E-5 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.074 1.75E-5 YCR016W_p YCR016W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis