YOR332W / VMA4

Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR332W / VMA4

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.33|P-value:1.45E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.52|P-value:3.36E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.44|P-value:4.38E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.55|P-value:6.76E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:-4.71|P-value:2.69E-6 ID:SGTC_444|Compound:curcumin|FD-Score:4.78|P-value:2.49E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.58|P-value:6.11E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.46|P-value:4.85E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.20|P-value:2.96E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.56|P-value:3.44E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.21|P-value:2.91E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:4.24|P-value:2.53E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.28|P-value:8.85E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.54|P-value:3.13E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.30|P-value:8.10E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.40|P-value:5.91E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.89|P-value:8.40E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.49|P-value:8.90E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.51|P-value:4.03E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-5.06|P-value:5.24E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.38|P-value:6.23E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.61|P-value:5.31E-6 ID:SGTC_1365|Compound:1672-3266|FD-Score:-4.37|P-value:1.21E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.44|P-value:5.12E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.48|P-value:4.46E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.43|P-value:5.37E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.30|P-value:7.11E-4 ID:SGTC_1893|Compound:st059353|FD-Score:-3.42|P-value:4.65E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.44|P-value:9.79E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.35|P-value:6.88E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.44|P-value:4.37E-4 ID:SGTC_2449|Compound:5812982|FD-Score:4.23|P-value:2.62E-5 ID:SGTC_2452|Compound:5863136|FD-Score:9.51|P-value:3.86E-20 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.61|P-value:2.85E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.48|P-value:9.41E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.98|P-value:6.03E-5 ID:SGTC_2818|Compound:7996756|FD-Score:3.54|P-value:3.63E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.28|P-value:7.58E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-4.07|P-value:4.12E-5 ID:SGTC_2895|Compound:9054447|FD-Score:3.57|P-value:3.28E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.51|P-value:4.09E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.37|P-value:5.49E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-4.00|P-value:5.51E-5 ID:SGTC_2981|Compound:9013952|FD-Score:4.40|P-value:1.28E-5 ID:SGTC_2997|Compound:9067075|FD-Score:4.46|P-value:9.97E-6 ID:SGTC_3036|Compound:9090181|FD-Score:3.63|P-value:2.66E-4 ID:SGTC_3074|Compound:9116018|FD-Score:4.18|P-value:3.20E-5 ID:SGTC_3089|Compound:9117425|FD-Score:6.15|P-value:1.94E-9 ID:SGTC_3153|Compound:9100258|FD-Score:3.70|P-value:2.09E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.33|P-value:1.45E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.52|P-value:3.36E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.44|P-value:4.38E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.55|P-value:6.76E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:-4.71|P-value:2.69E-6 ID:SGTC_444|Compound:curcumin|FD-Score:4.78|P-value:2.49E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.58|P-value:6.11E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.46|P-value:4.85E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.20|P-value:2.96E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.56|P-value:3.44E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.21|P-value:2.91E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:4.24|P-value:2.53E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.28|P-value:8.85E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.54|P-value:3.13E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.30|P-value:8.10E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.40|P-value:5.91E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.89|P-value:8.40E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.49|P-value:8.90E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.51|P-value:4.03E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-5.06|P-value:5.24E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.38|P-value:6.23E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.61|P-value:5.31E-6 ID:SGTC_1365|Compound:1672-3266|FD-Score:-4.37|P-value:1.21E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.44|P-value:5.12E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.48|P-value:4.46E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.43|P-value:5.37E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.30|P-value:7.11E-4 ID:SGTC_1893|Compound:st059353|FD-Score:-3.42|P-value:4.65E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.44|P-value:9.79E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.35|P-value:6.88E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.44|P-value:4.37E-4 ID:SGTC_2449|Compound:5812982|FD-Score:4.23|P-value:2.62E-5 ID:SGTC_2452|Compound:5863136|FD-Score:9.51|P-value:3.86E-20 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.61|P-value:2.85E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.48|P-value:9.41E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.98|P-value:6.03E-5 ID:SGTC_2818|Compound:7996756|FD-Score:3.54|P-value:3.63E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.28|P-value:7.58E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-4.07|P-value:4.12E-5 ID:SGTC_2895|Compound:9054447|FD-Score:3.57|P-value:3.28E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.51|P-value:4.09E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.37|P-value:5.49E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-4.00|P-value:5.51E-5 ID:SGTC_2981|Compound:9013952|FD-Score:4.40|P-value:1.28E-5 ID:SGTC_2997|Compound:9067075|FD-Score:4.46|P-value:9.97E-6 ID:SGTC_3036|Compound:9090181|FD-Score:3.63|P-value:2.66E-4 ID:SGTC_3074|Compound:9116018|FD-Score:4.18|P-value:3.20E-5 ID:SGTC_3089|Compound:9117425|FD-Score:6.15|P-value:1.94E-9 ID:SGTC_3153|Compound:9100258|FD-Score:3.70|P-value:2.09E-4

Top fitness defect scores for YOR332W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2452 5863136 9.51 48.4 µM 3.86E-20 1 9.38E-22
2 SGTC_3089 9117425 6.15 49.5 µM 1.94E-9 1 3.81E-10
3 SGTC_2418 pentamidine 5.44 31.7 µM 9.79E-8 mitochondrial processes 21 2.69E-8
4 SGTC_444 curcumin 4.78 90.4 µM 2.49E-6 iron homeostasis 11 8.98E-7
5 SGTC_1362 1611-0397 4.61 101.0 µM 5.31E-6 8 2.04E-6
6 SGTC_453 enantio-paf c16 4.58 10.0 µM 6.11E-6 105 2.38E-6
7 SGTC_236 1509-0011 4.55 5.3 µM 6.76E-6 356 2.65E-6
8 SGTC_1244 0416-0015 4.49 52.5 µM 8.90E-6 ERG2 18 3.58E-6
9 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 4.48 15.3 µM 9.41E-6 20 3.80E-6
10 SGTC_2997 9067075 4.46 71.4 µM 9.97E-6 unfolded protein response 41 4.05E-6
11 SGTC_2981 9013952 4.40 39.0 µM 1.28E-5 33 5.33E-6
12 SGTC_914 3820-6779 4.24 408.0 µM 2.53E-5 15 1.11E-5
13 SGTC_2449 5812982 4.23 45.8 µM 2.62E-5 5 1.15E-5
14 SGTC_912 3474-0017 4.21 2.2 µM 2.91E-5 18 1.29E-5
15 SGTC_457 grayanotoxin iii 4.20 135.0 µM 2.96E-5 56 1.32E-5
16 SGTC_3074 9116018 4.18 49.5 µM 3.20E-5 RNA processing & uracil transport 17 1.43E-5
17 SGTC_3153 9100258 3.70 49.5 µM 2.09E-4 30 1.10E-4
18 SGTC_3036 9090181 3.63 49.5 µM 2.66E-4 7 1.43E-4
19 SGTC_2539 epi(13)torulosol 3.61 80.0 µM 2.85E-4 37 1.54E-4
20 SGTC_2895 9054447 3.57 58.4 µM 3.28E-4 16 1.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 3.34E-13 YDR271C_d YDR271C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
0.114 3.48E-11 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.108 3.59E-10 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.107 5.37E-10 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
0.102 3.34E-9 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.100 6.06E-9 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.099 8.27E-9 YDL068W_d YDL068W_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.099 1.01E-8 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.096 2.75E-8 YLL033W IRC19 hom Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.096 3.01E-8 YGR192C TDH3 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication
0.096 3.06E-8 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.094 5.55E-8 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.093 6.17E-8 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.092 9.90E-8 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.092 1.08E-7 YDL085C-A_p YDL085C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus