YOR335C / ALA1

Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR335C / ALA1

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-4.54|P-value:1.03E-7 ID:SGTC_54|Compound:0159-0055|FD-Score:-3.39|P-value:5.40E-5 ID:SGTC_224|Compound:benfluorex|FD-Score:-3.32|P-value:7.29E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.83|P-value:6.02E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-3.85|P-value:5.44E-6 ID:SGTC_303|Compound:1348-1668|FD-Score:3.27|P-value:8.77E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:3.67|P-value:1.33E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:2.93|P-value:3.90E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.18|P-value:1.39E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.47|P-value:3.44E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:2.77|P-value:7.50E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:-2.74|P-value:8.85E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:2.83|P-value:5.85E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:2.75|P-value:8.14E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-2.78|P-value:7.46E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:-2.74|P-value:8.74E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.05|P-value:2.39E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:2.91|P-value:4.19E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:2.86|P-value:5.26E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:2.89|P-value:4.64E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-2.94|P-value:3.83E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.14|P-value:1.62E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.23|P-value:1.11E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:2.99|P-value:2.99E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.00|P-value:2.95E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:-2.96|P-value:3.61E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:2.72|P-value:8.94E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.07|P-value:2.22E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.63|P-value:1.56E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.98|P-value:2.51E-6 ID:SGTC_1727|Compound:st034309|FD-Score:3.97|P-value:2.70E-6 ID:SGTC_1780|Compound:st038448|FD-Score:4.08|P-value:1.48E-6 ID:SGTC_1814|Compound:st052110|FD-Score:-2.87|P-value:5.25E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.28|P-value:8.32E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-2.84|P-value:5.89E-4 ID:SGTC_2019|Compound:4016004|FD-Score:2.71|P-value:9.41E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.63|P-value:1.58E-5 ID:SGTC_2094|Compound:5332512|FD-Score:2.93|P-value:3.86E-4 ID:SGTC_2149|Compound:5554786|FD-Score:-2.89|P-value:4.75E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.77|P-value:7.60E-6 ID:SGTC_2216|Compound:7189485|FD-Score:4.38|P-value:2.52E-7 ID:SGTC_2240|Compound:6690706|FD-Score:2.94|P-value:3.73E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.14|P-value:1.60E-4 ID:SGTC_2308|Compound:7584454|FD-Score:4.34|P-value:3.33E-7 ID:SGTC_2310|Compound:7668677|FD-Score:3.49|P-value:3.18E-5 ID:SGTC_2312|Compound:7706453|FD-Score:4.36|P-value:2.98E-7 ID:SGTC_2337|Compound:9009472|FD-Score:2.94|P-value:3.71E-4 ID:SGTC_2393|Compound:9031399|FD-Score:2.83|P-value:5.83E-4 ID:SGTC_2425|Compound:5628681|FD-Score:2.75|P-value:8.06E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.45|P-value:3.77E-5 ID:SGTC_2453|Compound:5731132|FD-Score:3.53|P-value:2.60E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:2.71|P-value:9.24E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:4.17|P-value:8.57E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.77|P-value:7.94E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.27|P-value:9.00E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-2.73|P-value:8.90E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-4.49|P-value:1.42E-7 ID:SGTC_2843|Compound:9000894|FD-Score:4.68|P-value:4.19E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-5.53|P-value:1.25E-10 ID:SGTC_3062|Compound:9077494|FD-Score:-5.38|P-value:3.93E-10 ID:SGTC_3210|Compound:9128157|FD-Score:-4.46|P-value:1.70E-7 ID:SGTC_3221|Compound:9128209|FD-Score:-2.79|P-value:7.15E-4 ID:SGTC_3223|Compound:9129970|FD-Score:-3.57|P-value:2.26E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:-4.54|P-value:1.03E-7 ID:SGTC_54|Compound:0159-0055|FD-Score:-3.39|P-value:5.40E-5 ID:SGTC_224|Compound:benfluorex|FD-Score:-3.32|P-value:7.29E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.83|P-value:6.02E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-3.85|P-value:5.44E-6 ID:SGTC_303|Compound:1348-1668|FD-Score:3.27|P-value:8.77E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:3.67|P-value:1.33E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:2.93|P-value:3.90E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.18|P-value:1.39E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.47|P-value:3.44E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:2.77|P-value:7.50E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:-2.74|P-value:8.85E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:2.83|P-value:5.85E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:2.75|P-value:8.14E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-2.78|P-value:7.46E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:-2.74|P-value:8.74E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.05|P-value:2.39E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:2.91|P-value:4.19E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:2.86|P-value:5.26E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:2.89|P-value:4.64E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-2.94|P-value:3.83E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.14|P-value:1.62E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.23|P-value:1.11E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:2.99|P-value:2.99E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.00|P-value:2.95E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:-2.96|P-value:3.61E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:2.72|P-value:8.94E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.07|P-value:2.22E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.63|P-value:1.56E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.98|P-value:2.51E-6 ID:SGTC_1727|Compound:st034309|FD-Score:3.97|P-value:2.70E-6 ID:SGTC_1780|Compound:st038448|FD-Score:4.08|P-value:1.48E-6 ID:SGTC_1814|Compound:st052110|FD-Score:-2.87|P-value:5.25E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.28|P-value:8.32E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-2.84|P-value:5.89E-4 ID:SGTC_2019|Compound:4016004|FD-Score:2.71|P-value:9.41E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.63|P-value:1.58E-5 ID:SGTC_2094|Compound:5332512|FD-Score:2.93|P-value:3.86E-4 ID:SGTC_2149|Compound:5554786|FD-Score:-2.89|P-value:4.75E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.77|P-value:7.60E-6 ID:SGTC_2216|Compound:7189485|FD-Score:4.38|P-value:2.52E-7 ID:SGTC_2240|Compound:6690706|FD-Score:2.94|P-value:3.73E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.14|P-value:1.60E-4 ID:SGTC_2308|Compound:7584454|FD-Score:4.34|P-value:3.33E-7 ID:SGTC_2310|Compound:7668677|FD-Score:3.49|P-value:3.18E-5 ID:SGTC_2312|Compound:7706453|FD-Score:4.36|P-value:2.98E-7 ID:SGTC_2337|Compound:9009472|FD-Score:2.94|P-value:3.71E-4 ID:SGTC_2393|Compound:9031399|FD-Score:2.83|P-value:5.83E-4 ID:SGTC_2425|Compound:5628681|FD-Score:2.75|P-value:8.06E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.45|P-value:3.77E-5 ID:SGTC_2453|Compound:5731132|FD-Score:3.53|P-value:2.60E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:2.71|P-value:9.24E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:4.17|P-value:8.57E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.77|P-value:7.94E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.27|P-value:9.00E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-2.73|P-value:8.90E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-4.49|P-value:1.42E-7 ID:SGTC_2843|Compound:9000894|FD-Score:4.68|P-value:4.19E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-5.53|P-value:1.25E-10 ID:SGTC_3062|Compound:9077494|FD-Score:-5.38|P-value:3.93E-10 ID:SGTC_3210|Compound:9128157|FD-Score:-4.46|P-value:1.70E-7 ID:SGTC_3221|Compound:9128209|FD-Score:-2.79|P-value:7.15E-4 ID:SGTC_3223|Compound:9129970|FD-Score:-3.57|P-value:2.26E-5

Top fitness defect scores for YOR335C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2843 9000894 4.68 45.5 µM 4.19E-8 1 1.46E-6 0.84
2 SGTC_2216 7189485 4.38 200.0 µM 2.52E-7 2 5.81E-6 0.70
3 SGTC_2312 7706453 4.36 155.7 µM 2.98E-7 5 6.62E-6 0.16
4 SGTC_2308 7584454 4.34 197.6 µM 3.33E-7 7 7.21E-6 0.20
5 SGTC_2607 solasodine 4.17 47.4 µM 8.57E-7 10 1.49E-5 0.17
6 SGTC_1780 st038448 4.08 24.5 µM 1.48E-6 iron homeostasis 5 2.28E-5 0.02
7 SGTC_1599 8-chlro-1-tetrahydronorharmanone 3.98 90.6 µM 2.51E-6 8 3.42E-5 0.01
8 SGTC_1727 st034309 3.97 9.9 µM 2.70E-6 15 3.61E-5 0.14
9 SGTC_2151 5563267 3.77 85.4 µM 7.60E-6 21 8.05E-5 0.06
10 SGTC_2611 pyromycin 3.77 23.9 µM 7.94E-6 8 8.32E-5 0.06
11 SGTC_335 4077-0256 3.67 27.6 µM 1.33E-5 6 1.24E-4 0.34
12 SGTC_1578 josamycin 3.63 24.2 µM 1.56E-5 9 1.40E-4 0.43
13 SGTC_2068 5226020 3.63 147.0 µM 1.58E-5 9 1.41E-4 0.00
14 SGTC_2453 5731132 3.53 22.2 µM 2.60E-5 9 2.08E-4 0.13
15 SGTC_2310 7668677 3.49 200.0 µM 3.18E-5 9 2.43E-4 0.01
16 SGTC_558 k064-0027 3.47 21.3 µM 3.44E-5 DNA damage response 16 2.58E-4 0.09
17 SGTC_2428 5615643 3.45 7.8 µM 3.77E-5 9 2.77E-4 0.06
18 SGTC_1894 3',6'-dihydroxyflavone 3.28 78.7 µM 8.32E-5 9 5.12E-4 0.00
19 SGTC_303 1348-1668 3.27 34.2 µM 8.77E-5 8 5.33E-4 0.05
20 SGTC_2621 lithochol-11-enic acid 3.27 100.0 µM 9.00E-5 14 5.44E-4 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.80E-19 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.143 7.64E-17 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.134 6.42E-15 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.126 1.98E-13 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.124 5.11E-13 YDR021W FAL1 het Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
0.123 6.95E-13 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.121 2.14E-12 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.113 5.71E-11 YOR056C NOB1 het Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
0.110 1.75E-10 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.109 2.49E-10 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.105 1.14E-9 YDR373W FRQ1 het N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
0.103 2.22E-9 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.102 3.08E-9 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.100 6.60E-9 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.099 9.74E-9 YGL254W FZF1 hom Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress