YOR336W / KRE5

Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR336W / KRE5

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:2.99|P-value:8.39E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.71|P-value:3.87E-7 ID:SGTC_137|Compound:0438-0332|FD-Score:6.85|P-value:3.46E-13 ID:SGTC_144|Compound:4161-2749|FD-Score:3.08|P-value:5.93E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.94|P-value:1.07E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.33|P-value:3.21E-6 ID:SGTC_786|Compound:4544-0069|FD-Score:3.20|P-value:3.81E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.11|P-value:5.33E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:4.34|P-value:2.59E-6 ID:SGTC_917|Compound:harmaline|FD-Score:4.09|P-value:8.53E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:-3.15|P-value:5.14E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.10|P-value:8.50E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.67|P-value:6.58E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.02|P-value:7.50E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.69|P-value:5.33E-5 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.67|P-value:5.73E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.48|P-value:1.27E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.61|P-value:8.38E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.40|P-value:1.96E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.05|P-value:6.73E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.28|P-value:2.84E-4 ID:SGTC_1547|Compound:harmine|FD-Score:2.98|P-value:8.52E-4 ID:SGTC_1667|Compound:st014849|FD-Score:3.07|P-value:6.14E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.24|P-value:3.24E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.31|P-value:3.03E-6 ID:SGTC_1885|Compound:st057546|FD-Score:3.38|P-value:1.92E-4 ID:SGTC_1907|Compound:st060210|FD-Score:4.14|P-value:6.72E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.54|P-value:9.56E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.37|P-value:2.26E-4 ID:SGTC_1935|Compound:st074532|FD-Score:6.20|P-value:4.04E-11 ID:SGTC_1942|Compound:st076330|FD-Score:2.96|P-value:9.39E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.90|P-value:2.06E-5 ID:SGTC_1959|Compound:st077091|FD-Score:3.70|P-value:5.07E-5 ID:SGTC_2053|Compound:5265679|FD-Score:-2.98|P-value:9.68E-4 ID:SGTC_2183|Compound:6420625|FD-Score:4.28|P-value:3.48E-6 ID:SGTC_2330|Compound:7979373|FD-Score:3.35|P-value:2.15E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.20|P-value:4.36E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:4.14|P-value:6.85E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.88|P-value:1.47E-7 ID:SGTC_2796|Compound:7949634|FD-Score:4.30|P-value:3.20E-6 ID:SGTC_2894|Compound:9048625|FD-Score:5.76|P-value:7.35E-10 ID:SGTC_2968|Compound:9090663|FD-Score:3.15|P-value:4.71E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.28|P-value:2.87E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.78|P-value:3.64E-5 ID:SGTC_3127|Compound:9126946|FD-Score:4.48|P-value:1.31E-6 ID:SGTC_3251|Compound:9135047|FD-Score:4.24|P-value:4.26E-6 ID:SGTC_3302|Compound:9125395|FD-Score:3.37|P-value:1.96E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.19|P-value:3.94E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-4.38|P-value:2.51E-6 ID:SGTC_42|Compound:0133-0086|FD-Score:2.99|P-value:8.39E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.71|P-value:3.87E-7 ID:SGTC_137|Compound:0438-0332|FD-Score:6.85|P-value:3.46E-13 ID:SGTC_144|Compound:4161-2749|FD-Score:3.08|P-value:5.93E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.94|P-value:1.07E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.33|P-value:3.21E-6 ID:SGTC_786|Compound:4544-0069|FD-Score:3.20|P-value:3.81E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.11|P-value:5.33E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:4.34|P-value:2.59E-6 ID:SGTC_917|Compound:harmaline|FD-Score:4.09|P-value:8.53E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:-3.15|P-value:5.14E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.10|P-value:8.50E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.67|P-value:6.58E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.02|P-value:7.50E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.69|P-value:5.33E-5 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.67|P-value:5.73E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.48|P-value:1.27E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.61|P-value:8.38E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.40|P-value:1.96E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.05|P-value:6.73E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.28|P-value:2.84E-4 ID:SGTC_1547|Compound:harmine|FD-Score:2.98|P-value:8.52E-4 ID:SGTC_1667|Compound:st014849|FD-Score:3.07|P-value:6.14E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.24|P-value:3.24E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.31|P-value:3.03E-6 ID:SGTC_1885|Compound:st057546|FD-Score:3.38|P-value:1.92E-4 ID:SGTC_1907|Compound:st060210|FD-Score:4.14|P-value:6.72E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.54|P-value:9.56E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.37|P-value:2.26E-4 ID:SGTC_1935|Compound:st074532|FD-Score:6.20|P-value:4.04E-11 ID:SGTC_1942|Compound:st076330|FD-Score:2.96|P-value:9.39E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.90|P-value:2.06E-5 ID:SGTC_1959|Compound:st077091|FD-Score:3.70|P-value:5.07E-5 ID:SGTC_2053|Compound:5265679|FD-Score:-2.98|P-value:9.68E-4 ID:SGTC_2183|Compound:6420625|FD-Score:4.28|P-value:3.48E-6 ID:SGTC_2330|Compound:7979373|FD-Score:3.35|P-value:2.15E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.20|P-value:4.36E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:4.14|P-value:6.85E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.88|P-value:1.47E-7 ID:SGTC_2796|Compound:7949634|FD-Score:4.30|P-value:3.20E-6 ID:SGTC_2894|Compound:9048625|FD-Score:5.76|P-value:7.35E-10 ID:SGTC_2968|Compound:9090663|FD-Score:3.15|P-value:4.71E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.28|P-value:2.87E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.78|P-value:3.64E-5 ID:SGTC_3127|Compound:9126946|FD-Score:4.48|P-value:1.31E-6 ID:SGTC_3251|Compound:9135047|FD-Score:4.24|P-value:4.26E-6 ID:SGTC_3302|Compound:9125395|FD-Score:3.37|P-value:1.96E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.19|P-value:3.94E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-4.38|P-value:2.51E-6

Top fitness defect scores for YOR336W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_137 0438-0332 6.85 85.4 µM 3.46E-13 ubiquinone biosynthesis & proteasome 17 3.75E-12 0.14
2 SGTC_1935 st074532 6.20 41.6 µM 4.04E-11 1 2.89E-10 0.75
3 SGTC_2894 9048625 5.76 32.5 µM 7.35E-10 1 4.09E-9 0.55
4 SGTC_148 3253-0744 4.94 288.0 µM 1.07E-7 3 3.85E-7 0.01
5 SGTC_2620 irigenin 4.88 100.0 µM 1.47E-7 5 5.18E-7 0.02
6 SGTC_135 0933-0004 4.71 26.4 µM 3.87E-7 60S ribosome export 3 1.25E-6 0.21
7 SGTC_1926 methyl fluorone black 4.54 53.6 µM 9.56E-7 DNA intercalators 23 2.86E-6 0.29
8 SGTC_1446 4239-0190 4.48 62.0 µM 1.27E-6 6 3.72E-6 0.23
9 SGTC_3127 9126946 4.48 49.5 µM 1.31E-6 ERG2 9 3.81E-6 0.36
10 SGTC_1479 0549-0158 4.40 134.0 µM 1.96E-6 2 5.52E-6 0.45
11 SGTC_896 0964-0007 4.34 44.5 µM 2.59E-6 5 7.12E-6 0.06
12 SGTC_1768 st044301 4.31 37.2 µM 3.03E-6 4 8.21E-6 0.07
13 SGTC_2796 7949634 4.30 45.5 µM 3.20E-6 4 8.64E-6 0.25
14 SGTC_2183 6420625 4.28 130.5 µM 3.48E-6 mitochondrial processes 13 9.32E-6 0.23
15 SGTC_3251 9135047 4.24 49.5 µM 4.26E-6 60S ribosome export 4 1.12E-5 0.23
16 SGTC_1907 st060210 4.14 83.7 µM 6.72E-6 8 1.70E-5 0.31
17 SGTC_2610 ononetin 4.14 44.2 µM 6.85E-6 6 1.73E-5 0.44
18 SGTC_1373 2887-0003 4.10 58.5 µM 8.50E-6 Golgi 11 2.11E-5 0.18
19 SGTC_917 harmaline 4.09 728.1 nM 8.53E-6 ergosterol biosynthesis 9 2.11E-5 0.23
20 SGTC_1945 st075991 3.90 56.6 µM 2.06E-5 60S ribosome export 7 4.73E-5 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.89E-13 YLR379W_d YLR379W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C
0.112 8.22E-11 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.109 2.81E-10 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.107 4.56E-10 YOR165W SEY1 hom Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
0.106 8.16E-10 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.106 8.29E-10 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.103 1.86E-9 YPL151C PRP46 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
0.098 1.36E-8 YMR205C PFK2 hom Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
0.090 1.99E-7 YKL018W SWD2 het Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
0.089 2.82E-7 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.087 4.16E-7 YGR123C PPT1 hom Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
0.086 6.94E-7 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.085 7.09E-7 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.084 1.15E-6 YML018C YML018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
0.081 2.53E-6 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene