YOR348C / PUT4

Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR348C / PUT4

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.60|P-value:4.15E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.02|P-value:9.31E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-3.69|P-value:3.09E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:4.90|P-value:2.71E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:4.34|P-value:2.73E-5 ID:SGTC_532|Compound:1548-0774|FD-Score:4.06|P-value:8.06E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:4.77|P-value:4.71E-6 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.42|P-value:7.35E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:4.03|P-value:8.99E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:7.04|P-value:3.00E-11 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.62|P-value:3.93E-4 ID:SGTC_1738|Compound:st035555|FD-Score:4.58|P-value:1.07E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.42|P-value:7.38E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.54|P-value:5.02E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.89|P-value:1.50E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.49|P-value:5.89E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-4.86|P-value:3.20E-6 ID:SGTC_2271|Compound:7849410|FD-Score:-4.09|P-value:7.29E-5 ID:SGTC_2457|Compound:5731572|FD-Score:-3.37|P-value:8.87E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.39|P-value:8.30E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.99|P-value:1.06E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:4.21|P-value:4.58E-5 ID:SGTC_2740|Compound:felodipine|FD-Score:4.56|P-value:1.12E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.81|P-value:3.89E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.74|P-value:2.57E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.51|P-value:5.50E-4 ID:SGTC_2923|Compound:7992654|FD-Score:4.21|P-value:4.66E-5 ID:SGTC_2924|Compound:9010168|FD-Score:3.85|P-value:1.75E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.90|P-value:1.47E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.62|P-value:9.05E-6 ID:SGTC_3204|Compound:9114183|FD-Score:4.46|P-value:1.71E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.71|P-value:2.83E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.40|P-value:8.02E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.60|P-value:4.15E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.02|P-value:9.31E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-3.69|P-value:3.09E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:4.90|P-value:2.71E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:4.34|P-value:2.73E-5 ID:SGTC_532|Compound:1548-0774|FD-Score:4.06|P-value:8.06E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:4.77|P-value:4.71E-6 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.42|P-value:7.35E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:4.03|P-value:8.99E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:7.04|P-value:3.00E-11 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.62|P-value:3.93E-4 ID:SGTC_1738|Compound:st035555|FD-Score:4.58|P-value:1.07E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.42|P-value:7.38E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.54|P-value:5.02E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.89|P-value:1.50E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.49|P-value:5.89E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-4.86|P-value:3.20E-6 ID:SGTC_2271|Compound:7849410|FD-Score:-4.09|P-value:7.29E-5 ID:SGTC_2457|Compound:5731572|FD-Score:-3.37|P-value:8.87E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.39|P-value:8.30E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.99|P-value:1.06E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:4.21|P-value:4.58E-5 ID:SGTC_2740|Compound:felodipine|FD-Score:4.56|P-value:1.12E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.81|P-value:3.89E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.74|P-value:2.57E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.51|P-value:5.50E-4 ID:SGTC_2923|Compound:7992654|FD-Score:4.21|P-value:4.66E-5 ID:SGTC_2924|Compound:9010168|FD-Score:3.85|P-value:1.75E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.90|P-value:1.47E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.62|P-value:9.05E-6 ID:SGTC_3204|Compound:9114183|FD-Score:4.46|P-value:1.71E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.71|P-value:2.83E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.40|P-value:8.02E-4

Top fitness defect scores for YOR348C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1492 4158-0558 7.04 28.5 µM 3.00E-11 60S ribosome export 2 9.28E-13
2 SGTC_527 4476-4215 4.90 268.0 µM 2.71E-6 PDR1 17 4.88E-7
3 SGTC_2851 9015922 4.81 71.4 µM 3.89E-6 18 7.41E-7
4 SGTC_736 0958-0254 4.77 92.6 µM 4.71E-6 Golgi 13 9.23E-7
5 SGTC_3054 9094397 4.62 49.5 µM 9.05E-6 fatty acid desaturase (OLE1) 16 1.96E-6
6 SGTC_1738 st035555 4.58 74.0 µM 1.07E-5 TSC3-RPN4 19 2.37E-6
7 SGTC_2740 felodipine 4.56 17.1 µM 1.12E-5 14 2.51E-6
8 SGTC_3204 9114183 4.46 49.5 µM 1.71E-5 20 4.09E-6
9 SGTC_529 4076-0302 4.34 30.3 µM 2.73E-5 mitochondrial processes 12 6.97E-6
10 SGTC_2647 atorvastatin 4.21 60.2 µM 4.58E-5 azole & statin 14 1.27E-5
11 SGTC_2923 7992654 4.21 58.1 µM 4.66E-5 Golgi 20 1.29E-5
12 SGTC_532 1548-0774 4.06 215.0 µM 8.06E-5 16 2.43E-5
13 SGTC_1338 1486-1223 4.03 76.0 µM 8.99E-5 19 2.76E-5
14 SGTC_294 k038-0071 4.02 157.0 µM 9.31E-5 ubiquinone biosynthesis & proteasome 62 2.87E-5
15 SGTC_2639 lactacystin 3.99 53.0 µM 1.06E-4 20 3.35E-5
16 SGTC_2926 9010726 3.90 65.2 µM 1.47E-4 35 4.86E-5
17 SGTC_2924 9010168 3.85 62.8 µM 1.75E-4 cell wall signaling 44 5.91E-5
18 SGTC_2881 9058953 3.74 52.0 µM 2.57E-4 27 9.23E-5
19 SGTC_3205 9113483 3.71 49.5 µM 2.83E-4 35 1.03E-4
20 SGTC_1509 1326-1131 3.62 80.0 µM 3.93E-4 18 1.50E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.090 1.57E-7 YPL258C THI21 hom Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
0.089 2.61E-7 YCR036W RBK1 hom Putative ribokinase
0.085 8.99E-7 YMR003W AIM34 hom Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
0.084 1.09E-6 YJR004C SAG1 hom Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
0.084 1.20E-6 YKL133C_p YKL133C_p hom Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype)
0.081 2.54E-6 YDR438W THI74 hom Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication
0.081 2.88E-6 YNR054C ESF2 het Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
0.080 3.61E-6 YPL055C LGE1 hom Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
0.079 4.11E-6 YER114C BOI2 hom Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
0.079 4.76E-6 YDR045C RPC11 het RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
0.077 7.87E-6 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.076 9.33E-6 YDL149W ATG9 hom Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS
0.076 1.16E-5 YKR047W_d YKR047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
0.073 2.31E-5 YOR317W FAA1 hom Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
0.073 2.36E-5 YER053C PIC2 hom Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature