YOR352W / TFB6

Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR352W / TFB6

Click on Significant Values for Screen Details ID:SGTC_148|Compound:3253-0744|FD-Score:-3.90|P-value:3.34E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:3.38|P-value:2.80E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.00|P-value:2.28E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.41|P-value:2.54E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.21|P-value:5.25E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.42|P-value:2.39E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.19|P-value:5.77E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.19|P-value:5.46E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.17|P-value:6.10E-4 ID:SGTC_917|Compound:harmaline|FD-Score:5.24|P-value:4.40E-8 ID:SGTC_931|Compound:2812-0076|FD-Score:3.41|P-value:2.47E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.36|P-value:3.04E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.23|P-value:4.95E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.63|P-value:1.07E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:-4.16|P-value:1.07E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-4.44|P-value:2.89E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:5.05|P-value:1.25E-7 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.42|P-value:2.37E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.28|P-value:4.14E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.16|P-value:6.08E-4 ID:SGTC_1688|Compound:st021091|FD-Score:-3.08|P-value:8.29E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.38|P-value:2.70E-4 ID:SGTC_1722|Compound:st034304|FD-Score:3.75|P-value:6.53E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.27|P-value:4.30E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.14|P-value:6.77E-4 ID:SGTC_2049|Compound:5262831|FD-Score:4.32|P-value:5.17E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.11|P-value:7.51E-4 ID:SGTC_2134|Compound:5324836|FD-Score:10.40|P-value:1.18E-26 ID:SGTC_2218|Compound:6965428|FD-Score:3.15|P-value:6.60E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-3.05|P-value:9.14E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.63|P-value:1.04E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.43|P-value:2.31E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:3.52|P-value:1.61E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:4.26|P-value:7.02E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.66|P-value:9.55E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.25|P-value:4.57E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.76|P-value:6.23E-5 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-4.06|P-value:1.62E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.04|P-value:9.66E-4 ID:SGTC_2969|Compound:9091408|FD-Score:4.59|P-value:1.37E-6 ID:SGTC_3083|Compound:9117465|FD-Score:-3.19|P-value:5.56E-4 ID:SGTC_3101|Compound:9120531|FD-Score:-3.19|P-value:5.57E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.03|P-value:9.82E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-4.89|P-value:2.81E-7 ID:SGTC_3222|Compound:9128301|FD-Score:5.03|P-value:1.42E-7 ID:SGTC_3231|Compound:9132313|FD-Score:-3.75|P-value:6.31E-5 ID:SGTC_3233|Compound:9132340|FD-Score:-3.52|P-value:1.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:5.64|P-value:4.33E-9 ID:SGTC_3288|Compound:9119690|FD-Score:3.39|P-value:2.70E-4 ID:SGTC_3290|Compound:9121031|FD-Score:3.21|P-value:5.35E-4 ID:SGTC_3301|Compound:9125354|FD-Score:4.45|P-value:2.83E-6 ID:SGTC_3310|Compound:9130229|FD-Score:-3.66|P-value:9.14E-5 ID:SGTC_3332|Compound:9141069|FD-Score:4.52|P-value:1.99E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.90|P-value:3.34E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:3.38|P-value:2.80E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.00|P-value:2.28E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.41|P-value:2.54E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.21|P-value:5.25E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.42|P-value:2.39E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.19|P-value:5.77E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.19|P-value:5.46E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.17|P-value:6.10E-4 ID:SGTC_917|Compound:harmaline|FD-Score:5.24|P-value:4.40E-8 ID:SGTC_931|Compound:2812-0076|FD-Score:3.41|P-value:2.47E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.36|P-value:3.04E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.23|P-value:4.95E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.63|P-value:1.07E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:-4.16|P-value:1.07E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-4.44|P-value:2.89E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:5.05|P-value:1.25E-7 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.42|P-value:2.37E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.28|P-value:4.14E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.16|P-value:6.08E-4 ID:SGTC_1688|Compound:st021091|FD-Score:-3.08|P-value:8.29E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.38|P-value:2.70E-4 ID:SGTC_1722|Compound:st034304|FD-Score:3.75|P-value:6.53E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.27|P-value:4.30E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.14|P-value:6.77E-4 ID:SGTC_2049|Compound:5262831|FD-Score:4.32|P-value:5.17E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.11|P-value:7.51E-4 ID:SGTC_2134|Compound:5324836|FD-Score:10.40|P-value:1.18E-26 ID:SGTC_2218|Compound:6965428|FD-Score:3.15|P-value:6.60E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-3.05|P-value:9.14E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.63|P-value:1.04E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.43|P-value:2.31E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:3.52|P-value:1.61E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:4.26|P-value:7.02E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.66|P-value:9.55E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.25|P-value:4.57E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.76|P-value:6.23E-5 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-4.06|P-value:1.62E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.04|P-value:9.66E-4 ID:SGTC_2969|Compound:9091408|FD-Score:4.59|P-value:1.37E-6 ID:SGTC_3083|Compound:9117465|FD-Score:-3.19|P-value:5.56E-4 ID:SGTC_3101|Compound:9120531|FD-Score:-3.19|P-value:5.57E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.03|P-value:9.82E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-4.89|P-value:2.81E-7 ID:SGTC_3222|Compound:9128301|FD-Score:5.03|P-value:1.42E-7 ID:SGTC_3231|Compound:9132313|FD-Score:-3.75|P-value:6.31E-5 ID:SGTC_3233|Compound:9132340|FD-Score:-3.52|P-value:1.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:5.64|P-value:4.33E-9 ID:SGTC_3288|Compound:9119690|FD-Score:3.39|P-value:2.70E-4 ID:SGTC_3290|Compound:9121031|FD-Score:3.21|P-value:5.35E-4 ID:SGTC_3301|Compound:9125354|FD-Score:4.45|P-value:2.83E-6 ID:SGTC_3310|Compound:9130229|FD-Score:-3.66|P-value:9.14E-5 ID:SGTC_3332|Compound:9141069|FD-Score:4.52|P-value:1.99E-6

Top fitness defect scores for YOR352W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2134 5324836 10.40 1.5 µM 1.18E-26 2 1.22E-25
2 SGTC_3249 9135183 5.64 49.5 µM 4.33E-9 60S ribosome export 2 8.63E-9
3 SGTC_917 harmaline 5.24 728.1 nM 4.40E-8 ergosterol biosynthesis 9 7.99E-8
4 SGTC_1326 1431-2094 5.05 10.8 µM 1.25E-7 tubulin folding & SWR complex 17 2.17E-7
5 SGTC_3222 9128301 5.03 49.5 µM 1.42E-7 60S ribosome export 10 2.46E-7
6 SGTC_2969 9091408 4.59 28.7 µM 1.37E-6 13 2.17E-6
7 SGTC_3332 9141069 4.52 11.2 µM 1.99E-6 7 3.10E-6
8 SGTC_3301 9125354 4.45 19.8 µM 2.83E-6 8 4.36E-6
9 SGTC_2049 5262831 4.32 67.2 µM 5.17E-6 21 7.77E-6
10 SGTC_2607 solasodine 4.26 47.4 µM 7.02E-6 50 1.04E-5
11 SGTC_178 4092-0806 4.00 87.5 µM 2.28E-5 ergosterol depletion effects on membrane 97 3.23E-5
12 SGTC_2660 2',4'-dihydroxychalcone 3.76 17.1 µM 6.23E-5 21 8.50E-5
13 SGTC_1722 st034304 3.75 3.6 µM 6.53E-5 mitochondrial processes 32 8.90E-5
14 SGTC_2611 pyromycin 3.66 23.9 µM 9.55E-5 38 1.28E-4
15 SGTC_2444 5485484 3.63 36.4 µM 1.04E-4 43 1.39E-4
16 SGTC_1137 ixabepilone 3.63 100.0 µM 1.07E-4 39 1.43E-4
17 SGTC_2512 diallyl trisulfide 3.52 49.2 µM 1.61E-4 14 2.12E-4
18 SGTC_2510 benzyl isothiocyanate 3.43 8.3 µM 2.31E-4 29 3.00E-4
19 SGTC_1409 3909-8004 3.42 61.9 µM 2.37E-4 40 3.07E-4
20 SGTC_558 k064-0027 3.42 21.3 µM 2.39E-4 DNA damage response 44 3.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.28E-13 YMR190C SGS1 hom Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
0.093 7.14E-8 YGR118W RPS23A hom Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.088 3.48E-7 YOL007C CSI2 hom Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
0.085 7.34E-7 YHR072W ERG7 het Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
0.085 8.51E-7 YCL034W LSB5 hom Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat
0.081 2.64E-6 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.076 9.19E-6 YBR182C SMP1 hom Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
0.076 1.19E-5 YKL064W MNR2 hom Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
0.074 1.59E-5 YDR234W LYS4 hom Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
0.072 3.31E-5 YOR007C SGT2 hom Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
0.071 3.63E-5 YIR027C DAL1 hom Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
0.071 3.67E-5 YJR116W_p TDA4_p hom Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
0.071 4.10E-5 YOR264W DSE3 hom Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.070 4.96E-5 YDR058C TGL2 hom Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
0.069 6.28E-5 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids