HIPHOP chemogenomics database

YOR362C / PRE10

Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YOR362C / PRE10

Click on Significant Values for Screen Details

Top fitness defect scores for YOR362C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1597	7-benzyloxygramine	13.60	47.5 µM	2.36E-29	calcium & mitochondrial duress	11	3.05E-42	0.28
2	SGTC_137	0438-0332	9.60	85.4 µM	1.27E-15	ubiquinone biosynthesis & proteasome	9	3.95E-22	0.26
3	SGTC_64	000l-0018	7.84	272.1 µM	5.31E-11	ubiquinone biosynthesis & proteasome	20	2.26E-15	0.16
4	SGTC_2250	alverine citrate	5.96	130.0 µM	4.71E-7		17	1.29E-9	0.00
5	SGTC_1515	3460-0031	5.72	42.1 µM	1.22E-6	DNA intercalators	5	5.18E-9	1.57
6	SGTC_294	k038-0071	5.56	157.0 µM	2.34E-6	ubiquinone biosynthesis & proteasome	8	1.33E-8	0.16
7	SGTC_467	ro 31-8220	5.55	72.8 µM	2.48E-6	calcium & mitochondrial duress	8	1.44E-8	0.15
8	SGTC_158	k038-0087	5.53	26.4 µM	2.69E-6	ubiquinone biosynthesis & proteasome	16	1.63E-8	0.02
9	SGTC_1712	st032288	5.38	53.4 µM	4.71E-6	calcium & mitochondrial duress	19	3.67E-8	0.03
10	SGTC_142	0335-0849	5.10	62.7 µM	1.34E-5	ubiquinone biosynthesis & proteasome	13	1.67E-7	0.39
11	SGTC_2366	9070441	5.07	60.6 µM	1.53E-5		3	2.03E-7	0.29
12	SGTC_1880	MG-132	4.97	525.0 µM	2.19E-5		16	3.43E-7	0.42
13	SGTC_2401	5128683	4.85	200.0 µM	3.34E-5		4	6.30E-7	0.20
14	SGTC_1327	1436-0082	4.75	2.2 µM	4.70E-5		3	1.03E-6	0.02
15	SGTC_367	0335-0881	4.65	7.1 µM	6.62E-5	ubiquinone biosynthesis & proteasome	15	1.70E-6	0.07
16	SGTC_1599	8-chlro-1-tetrahydronorharmanone	4.54	90.6 µM	9.38E-5		6	2.81E-6	0.04
17	SGTC_165	k038-0023	4.51	64.4 µM	1.03E-4	ubiquinone biosynthesis & proteasome	18	3.21E-6	0.10
18	SGTC_1724	st037281	4.50	8.3 µM	1.08E-4		7	3.45E-6	0.03
19	SGTC_187	1488-2104	4.43	32.7 µM	1.35E-4	calcium & mitochondrial duress	6	4.75E-6	0.25
20	SGTC_2232	chlorophetanol	4.42	41.5 µM	1.37E-4	TRP & mitochondrial translation	6	4.84E-6	0.69

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.485	1.65E-197	YMR314W	PRE5	het	Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.478	2.68E-191	YER094C	PUP3	het	Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.455	5.00E-171	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.446	7.00E-164	YER012W	PRE1	het	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.426	7.80E-148	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.415	1.79E-139	YML092C	PRE8	het	Alpha 2 subunit of the 20S proteasome
0.403	4.58E-131	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.392	1.85E-123	YGL011C	SCL1	het	Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.318	1.81E-79	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.285	8.59E-64	YJL001W	PRE3	het	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.284	4.50E-63	YJL165C	HAL5	hom	Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.266	2.45E-55	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.265	7.18E-55	YPR103W	PRE2	het	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.251	1.79E-49	YDR086C	SSS1	het	Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.249	8.29E-49	YML034C-A_d	YML034C-A_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data