YOR362C / PRE10

Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR362C / PRE10

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:7.84|P-value:5.31E-11 ID:SGTC_137|Compound:0438-0332|FD-Score:9.60|P-value:1.27E-15 ID:SGTC_142|Compound:0335-0849|FD-Score:5.10|P-value:1.34E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:5.53|P-value:2.69E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.51|P-value:1.03E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.49|P-value:1.00E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.43|P-value:1.35E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:5.56|P-value:2.34E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:4.65|P-value:6.62E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.95|P-value:5.37E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:4.31|P-value:1.95E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.55|P-value:2.48E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:4.11|P-value:3.64E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.73|P-value:9.89E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.91|P-value:6.45E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:-4.27|P-value:2.01E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.98|P-value:5.34E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.84|P-value:8.07E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.83|P-value:8.30E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.39|P-value:1.39E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.75|P-value:4.70E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:-4.94|P-value:2.15E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:5.72|P-value:1.22E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:13.60|P-value:2.36E-29 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.54|P-value:9.38E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.38|P-value:4.71E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.81|P-value:8.64E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.50|P-value:1.08E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:4.97|P-value:2.19E-5 ID:SGTC_2016|Compound:5121758|FD-Score:3.90|P-value:6.76E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.23|P-value:2.48E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.41|P-value:1.43E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-4.73|P-value:4.51E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.87|P-value:7.24E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.42|P-value:1.37E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.96|P-value:4.71E-7 ID:SGTC_2358|Compound:9037157|FD-Score:4.17|P-value:3.00E-4 ID:SGTC_2366|Compound:9070441|FD-Score:5.07|P-value:1.53E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.85|P-value:3.34E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-3.97|P-value:5.04E-4 ID:SGTC_2945|Compound:9067681|FD-Score:4.39|P-value:1.52E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.41|P-value:1.29E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.22|P-value:7.75E-6 ID:SGTC_3142|Compound:9086498|FD-Score:4.24|P-value:2.46E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:7.84|P-value:5.31E-11 ID:SGTC_137|Compound:0438-0332|FD-Score:9.60|P-value:1.27E-15 ID:SGTC_142|Compound:0335-0849|FD-Score:5.10|P-value:1.34E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:5.53|P-value:2.69E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.51|P-value:1.03E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.49|P-value:1.00E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.43|P-value:1.35E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:5.56|P-value:2.34E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:4.65|P-value:6.62E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.95|P-value:5.37E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:4.31|P-value:1.95E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.55|P-value:2.48E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:4.11|P-value:3.64E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.73|P-value:9.89E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.91|P-value:6.45E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:-4.27|P-value:2.01E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.98|P-value:5.34E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.84|P-value:8.07E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.83|P-value:8.30E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.39|P-value:1.39E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.75|P-value:4.70E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:-4.94|P-value:2.15E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:5.72|P-value:1.22E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:13.60|P-value:2.36E-29 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.54|P-value:9.38E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.38|P-value:4.71E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.81|P-value:8.64E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.50|P-value:1.08E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:4.97|P-value:2.19E-5 ID:SGTC_2016|Compound:5121758|FD-Score:3.90|P-value:6.76E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.23|P-value:2.48E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.41|P-value:1.43E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-4.73|P-value:4.51E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.87|P-value:7.24E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.42|P-value:1.37E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.96|P-value:4.71E-7 ID:SGTC_2358|Compound:9037157|FD-Score:4.17|P-value:3.00E-4 ID:SGTC_2366|Compound:9070441|FD-Score:5.07|P-value:1.53E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.85|P-value:3.34E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-3.97|P-value:5.04E-4 ID:SGTC_2945|Compound:9067681|FD-Score:4.39|P-value:1.52E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.41|P-value:1.29E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.22|P-value:7.75E-6 ID:SGTC_3142|Compound:9086498|FD-Score:4.24|P-value:2.46E-4

Top fitness defect scores for YOR362C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1597 7-benzyloxygramine 13.60 47.5 µM 2.36E-29 calcium & mitochondrial duress 11 3.05E-42 0.28
2 SGTC_137 0438-0332 9.60 85.4 µM 1.27E-15 ubiquinone biosynthesis & proteasome 9 3.95E-22 0.26
3 SGTC_64 000l-0018 7.84 272.1 µM 5.31E-11 ubiquinone biosynthesis & proteasome 20 2.26E-15 0.16
4 SGTC_2250 alverine citrate 5.96 130.0 µM 4.71E-7 17 1.29E-9 0.00
5 SGTC_1515 3460-0031 5.72 42.1 µM 1.22E-6 DNA intercalators 5 5.18E-9 1.57
6 SGTC_294 k038-0071 5.56 157.0 µM 2.34E-6 ubiquinone biosynthesis & proteasome 8 1.33E-8 0.16
7 SGTC_467 ro 31-8220 5.55 72.8 µM 2.48E-6 calcium & mitochondrial duress 8 1.44E-8 0.15
8 SGTC_158 k038-0087 5.53 26.4 µM 2.69E-6 ubiquinone biosynthesis & proteasome 16 1.63E-8 0.02
9 SGTC_1712 st032288 5.38 53.4 µM 4.71E-6 calcium & mitochondrial duress 19 3.67E-8 0.03
10 SGTC_142 0335-0849 5.10 62.7 µM 1.34E-5 ubiquinone biosynthesis & proteasome 13 1.67E-7 0.39
11 SGTC_2366 9070441 5.07 60.6 µM 1.53E-5 3 2.03E-7 0.29
12 SGTC_1880 MG-132 4.97 525.0 µM 2.19E-5 16 3.43E-7 0.42
13 SGTC_2401 5128683 4.85 200.0 µM 3.34E-5 4 6.30E-7 0.20
14 SGTC_1327 1436-0082 4.75 2.2 µM 4.70E-5 3 1.03E-6 0.02
15 SGTC_367 0335-0881 4.65 7.1 µM 6.62E-5 ubiquinone biosynthesis & proteasome 15 1.70E-6 0.07
16 SGTC_1599 8-chlro-1-tetrahydronorharmanone 4.54 90.6 µM 9.38E-5 6 2.81E-6 0.04
17 SGTC_165 k038-0023 4.51 64.4 µM 1.03E-4 ubiquinone biosynthesis & proteasome 18 3.21E-6 0.10
18 SGTC_1724 st037281 4.50 8.3 µM 1.08E-4 7 3.45E-6 0.03
19 SGTC_187 1488-2104 4.43 32.7 µM 1.35E-4 calcium & mitochondrial duress 6 4.75E-6 0.25
20 SGTC_2232 chlorophetanol 4.42 41.5 µM 1.37E-4 TRP & mitochondrial translation 6 4.84E-6 0.69

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.485 1.65E-197 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.478 2.68E-191 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.455 5.00E-171 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.446 7.00E-164 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.426 7.80E-148 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.415 1.79E-139 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.403 4.58E-131 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.392 1.85E-123 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.318 1.81E-79 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.285 8.59E-64 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.284 4.50E-63 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.266 2.45E-55 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.265 7.18E-55 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.251 1.79E-49 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.249 8.29E-49 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data