HIPHOP chemogenomics database

YOR371C / GPB1

Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR371C / GPB1

Click on Significant Values for Screen Details

Top fitness defect scores for YOR371C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1746	st038117	5.30	42.0 µM	1.77E-8		12	5.81E-8
2	SGTC_2620	irigenin	4.82	100.0 µM	2.74E-7		15	7.34E-7
3	SGTC_2794	7785382	4.53	27.3 µM	1.24E-6		16	2.97E-6
4	SGTC_1869	3',7'-dimethoxyflavone	4.46	40.3 µM	1.72E-6		16	4.03E-6
5	SGTC_2401	5128683	4.36	200.0 µM	2.86E-6		27	6.45E-6
6	SGTC_2583	asiatic acid	4.26	100.0 µM	4.74E-6		30	1.03E-5
7	SGTC_1192	1488-0095	4.17	21.4 µM	7.10E-6		13	1.50E-5
8	SGTC_2550	chrysin dimethyl ether	4.04	71.9 µM	1.31E-5		10	2.65E-5
9	SGTC_77	0828-0286	3.89	12.5 µM	2.59E-5	TSC3-RPN4	40	4.97E-5
10	SGTC_1183	1254-0324	3.79	198.0 µM	3.99E-5		25	7.43E-5
11	SGTC_263	alizarin	3.69	107.9 µM	6.13E-5	iron homeostasis	68	1.11E-4
12	SGTC_3351	9150312	3.69	21.6 µM	6.25E-5	cell wall	39	1.13E-4
13	SGTC_376	3970-0862	3.65	59.1 µM	7.23E-5		26	1.29E-4
14	SGTC_1551	6-bromo-3'-methylflavone	3.57	63.5 µM	1.02E-4		32	1.77E-4
15	SGTC_2238	6671620	3.55	73.2 µM	1.13E-4		41	1.94E-4
16	SGTC_330	1488-0319	3.50	80.1 µM	1.38E-4	RPP1 & pyrimidine depletion	20	2.35E-4
17	SGTC_1284	0929-0043	3.48	16.0 µM	1.46E-4		40	2.47E-4
18	SGTC_977	1348-1378	3.47	135.0 µM	1.57E-4		34	2.64E-4
19	SGTC_958	1222-0007	3.45	33.0 µM	1.66E-4		46	2.79E-4
20	SGTC_865	0488-0499	3.33	301.0 µM	2.62E-4		35	4.27E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.118	7.51E-12	YPL151C	PRP46	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
0.115	2.64E-11	YOL139C	CDC33	het	mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
0.104	1.36E-9	YNL038W	GPI15	het	Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
0.101	4.27E-9	YMR281W	GPI12	het	ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
0.098	1.28E-8	YDL171C	GLT1	hom	NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
0.096	2.23E-8	YGR082W	TOM20	het	Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.095	3.67E-8	YNL250W	RAD50	hom	Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
0.086	6.08E-7	YOR292C_p	YOR292C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
0.084	1.27E-6	YLL061W	MMP1	hom	High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
0.083	1.59E-6	YFL009W	CDC4	het	F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.082	2.24E-6	YLR117C	CLF1	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.081	2.64E-6	YAL018C_p	YAL018C_p	hom	Putative protein of unknown function
0.079	5.28E-6	YJL010C	NOP9	het	Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
0.078	6.16E-6	YHR015W	MIP6	hom	Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.076	1.16E-5	YDL238C	GUD1	hom	Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures