YOR371C / GPB1

Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR371C / GPB1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.13|P-value:5.62E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-4.17|P-value:6.95E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.89|P-value:2.59E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.69|P-value:6.13E-5 ID:SGTC_264|Compound:promethazine|FD-Score:2.98|P-value:9.58E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.50|P-value:1.38E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.65|P-value:7.23E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.76|P-value:4.54E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.01|P-value:8.81E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:3.29|P-value:3.13E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:-3.08|P-value:6.64E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.24|P-value:3.70E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.33|P-value:2.62E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:3.19|P-value:4.51E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.45|P-value:1.66E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.47|P-value:1.57E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.49|P-value:1.41E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.89|P-value:2.52E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.79|P-value:3.99E-5 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.17|P-value:7.10E-6 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.48|P-value:1.46E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.57|P-value:1.02E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.61|P-value:8.64E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.07|P-value:6.84E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.01|P-value:8.63E-4 ID:SGTC_1636|Compound:st007707|FD-Score:-3.04|P-value:7.78E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.26|P-value:3.48E-4 ID:SGTC_1746|Compound:st038117|FD-Score:5.30|P-value:1.77E-8 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:4.46|P-value:1.72E-6 ID:SGTC_1874|Compound:staurosporine|FD-Score:2.99|P-value:9.48E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.55|P-value:1.13E-4 ID:SGTC_2259|Compound:7964009|FD-Score:-3.48|P-value:1.47E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.97|P-value:1.76E-5 ID:SGTC_2397|Compound:5161689|FD-Score:-3.40|P-value:2.02E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.36|P-value:2.86E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:4.04|P-value:1.31E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.26|P-value:4.74E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.10|P-value:6.14E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:4.82|P-value:2.74E-7 ID:SGTC_2794|Compound:7785382|FD-Score:4.53|P-value:1.24E-6 ID:SGTC_2807|Compound:7945238|FD-Score:-3.38|P-value:2.14E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.06|P-value:7.26E-4 ID:SGTC_3351|Compound:9150312|FD-Score:3.69|P-value:6.25E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.13|P-value:5.62E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-4.17|P-value:6.95E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.89|P-value:2.59E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.69|P-value:6.13E-5 ID:SGTC_264|Compound:promethazine|FD-Score:2.98|P-value:9.58E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.50|P-value:1.38E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.65|P-value:7.23E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.76|P-value:4.54E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.01|P-value:8.81E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:3.29|P-value:3.13E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:-3.08|P-value:6.64E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.24|P-value:3.70E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.33|P-value:2.62E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:3.19|P-value:4.51E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.45|P-value:1.66E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:3.47|P-value:1.57E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.49|P-value:1.41E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.89|P-value:2.52E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.79|P-value:3.99E-5 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.17|P-value:7.10E-6 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.48|P-value:1.46E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.57|P-value:1.02E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.61|P-value:8.64E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.07|P-value:6.84E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.01|P-value:8.63E-4 ID:SGTC_1636|Compound:st007707|FD-Score:-3.04|P-value:7.78E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-3.26|P-value:3.48E-4 ID:SGTC_1746|Compound:st038117|FD-Score:5.30|P-value:1.77E-8 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:4.46|P-value:1.72E-6 ID:SGTC_1874|Compound:staurosporine|FD-Score:2.99|P-value:9.48E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.55|P-value:1.13E-4 ID:SGTC_2259|Compound:7964009|FD-Score:-3.48|P-value:1.47E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.97|P-value:1.76E-5 ID:SGTC_2397|Compound:5161689|FD-Score:-3.40|P-value:2.02E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.36|P-value:2.86E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:4.04|P-value:1.31E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.26|P-value:4.74E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.10|P-value:6.14E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:4.82|P-value:2.74E-7 ID:SGTC_2794|Compound:7785382|FD-Score:4.53|P-value:1.24E-6 ID:SGTC_2807|Compound:7945238|FD-Score:-3.38|P-value:2.14E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.06|P-value:7.26E-4 ID:SGTC_3351|Compound:9150312|FD-Score:3.69|P-value:6.25E-5

Top fitness defect scores for YOR371C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1746 st038117 5.30 42.0 µM 1.77E-8 12 5.81E-8
2 SGTC_2620 irigenin 4.82 100.0 µM 2.74E-7 15 7.34E-7
3 SGTC_2794 7785382 4.53 27.3 µM 1.24E-6 16 2.97E-6
4 SGTC_1869 3',7'-dimethoxyflavone 4.46 40.3 µM 1.72E-6 16 4.03E-6
5 SGTC_2401 5128683 4.36 200.0 µM 2.86E-6 27 6.45E-6
6 SGTC_2583 asiatic acid 4.26 100.0 µM 4.74E-6 30 1.03E-5
7 SGTC_1192 1488-0095 4.17 21.4 µM 7.10E-6 13 1.50E-5
8 SGTC_2550 chrysin dimethyl ether 4.04 71.9 µM 1.31E-5 10 2.65E-5
9 SGTC_77 0828-0286 3.89 12.5 µM 2.59E-5 TSC3-RPN4 40 4.97E-5
10 SGTC_1183 1254-0324 3.79 198.0 µM 3.99E-5 25 7.43E-5
11 SGTC_263 alizarin 3.69 107.9 µM 6.13E-5 iron homeostasis 68 1.11E-4
12 SGTC_3351 9150312 3.69 21.6 µM 6.25E-5 cell wall 39 1.13E-4
13 SGTC_376 3970-0862 3.65 59.1 µM 7.23E-5 26 1.29E-4
14 SGTC_1551 6-bromo-3'-methylflavone 3.57 63.5 µM 1.02E-4 32 1.77E-4
15 SGTC_2238 6671620 3.55 73.2 µM 1.13E-4 41 1.94E-4
16 SGTC_330 1488-0319 3.50 80.1 µM 1.38E-4 RPP1 & pyrimidine depletion 20 2.35E-4
17 SGTC_1284 0929-0043 3.48 16.0 µM 1.46E-4 40 2.47E-4
18 SGTC_977 1348-1378 3.47 135.0 µM 1.57E-4 34 2.64E-4
19 SGTC_958 1222-0007 3.45 33.0 µM 1.66E-4 46 2.79E-4
20 SGTC_865 0488-0499 3.33 301.0 µM 2.62E-4 35 4.27E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 7.51E-12 YPL151C PRP46 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
0.115 2.64E-11 YOL139C CDC33 het mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
0.104 1.36E-9 YNL038W GPI15 het Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
0.101 4.27E-9 YMR281W GPI12 het ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
0.098 1.28E-8 YDL171C GLT1 hom NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
0.096 2.23E-8 YGR082W TOM20 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.095 3.67E-8 YNL250W RAD50 hom Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
0.086 6.08E-7 YOR292C_p YOR292C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
0.084 1.27E-6 YLL061W MMP1 hom High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
0.083 1.59E-6 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.082 2.24E-6 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.081 2.64E-6 YAL018C_p YAL018C_p hom Putative protein of unknown function
0.079 5.28E-6 YJL010C NOP9 het Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
0.078 6.16E-6 YHR015W MIP6 hom Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.076 1.16E-5 YDL238C GUD1 hom Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures