HIPHOP chemogenomics database

YOR375C / GDH1

NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YOR375C / GDH1

Click on Significant Values for Screen Details

Top fitness defect scores for YOR375C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2407	paclitaxel	5.11	400.0 µM	1.76E-7		14	1.63E-7
2	SGTC_746	1095-0025	5.11	46.6 µM	1.77E-7	RSC & ERG11	6	1.64E-7
3	SGTC_2582	agaric acid	5.01	100.0 µM	2.97E-7	redox potentiating	221	2.78E-7
4	SGTC_599	1935-0052	4.62	86.5 µM	2.02E-6		6	1.94E-6
5	SGTC_2787	5284540	4.56	71.4 µM	2.71E-6		12	2.62E-6
6	SGTC_1718	roemerine	4.26	11.9 µM	1.03E-5	redox potentiating	45	1.01E-5
7	SGTC_865	0488-0499	4.24	301.0 µM	1.15E-5		13	1.13E-5
8	SGTC_2430	5571206	4.19	200.0 µM	1.41E-5		49	1.39E-5
9	SGTC_1705	st027868	4.11	56.3 µM	1.99E-5		7	1.97E-5
10	SGTC_1739	st037196	4.04	92.5 µM	2.68E-5		19	2.66E-5
11	SGTC_1137	ixabepilone	3.96	100.0 µM	3.68E-5		23	3.68E-5
12	SGTC_1436	4092-1007	3.89	8.3 µM	5.09E-5		12	5.10E-5
13	SGTC_2309	7664088	3.81	163.9 µM	6.88E-5		37	6.92E-5
14	SGTC_2782	7736369	3.79	71.4 µM	7.38E-5		48	7.42E-5
15	SGTC_753	1322-0200	3.58	289.0 µM	1.70E-4		33	1.72E-4
16	SGTC_2391	6285546	3.57	200.0 µM	1.73E-4		27	1.76E-4
17	SGTC_2311	7694451	3.42	200.0 µM	3.06E-4		41	3.13E-4
18	SGTC_1977	st070720	3.34	65.3 µM	4.07E-4		23	4.17E-4
19	SGTC_372	0155-0182	3.31	15.9 µM	4.50E-4	mitochondrial response to ROS	50	4.61E-4
20	SGTC_2788	5282734	3.23	71.4 µM	6.04E-4		86	6.21E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.153	6.22E-19	YNL027W	CRZ1	hom	Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.149	4.68E-18	YBR082C	UBC4	hom	Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.142	1.23E-16	YNL106C	INP52	hom	Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.140	3.01E-16	YOR050C_d	YOR050C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.136	2.82E-15	YDL242W_d	YDL242W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.134	7.42E-15	YML103C	NUP188	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.126	2.09E-13	YOR049C	RSB1	hom	Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.122	1.27E-12	YML052W	SUR7	hom	Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.120	3.13E-12	YPL232W	SSO1	hom	Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.119	4.68E-12	YDR376W	ARH1	het	Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.114	3.21E-11	YEL007W	MIT1	hom	Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.114	3.94E-11	YMR109W	MYO5	hom	One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.113	4.89E-11	YKL213C	DOA1	hom	WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.111	1.39E-10	YGR144W	THI4	hom	Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.110	1.54E-10	YJL169W_d	YJL169W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2