YPL023C / MET12

Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL023C / MET12

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:3.49|P-value:3.10E-4 ID:SGTC_106|Compound:0092-0003|FD-Score:3.36|P-value:4.82E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.47|P-value:3.23E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.40|P-value:3.74E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.26|P-value:6.93E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-3.61|P-value:1.71E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:-5.46|P-value:3.29E-8 ID:SGTC_1136|Compound:pergolide|FD-Score:3.56|P-value:2.36E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.17|P-value:9.35E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.25|P-value:7.12E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.48|P-value:3.10E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-4.86|P-value:7.32E-7 ID:SGTC_1377|Compound:2944-0135|FD-Score:-5.22|P-value:1.17E-7 ID:SGTC_1442|Compound:4112-3290|FD-Score:4.32|P-value:1.11E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.97|P-value:4.80E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.66|P-value:1.62E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.86|P-value:7.54E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.91|P-value:6.07E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:4.33|P-value:1.04E-5 ID:SGTC_1488|Compound:4013-0813|FD-Score:3.49|P-value:3.05E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.18|P-value:9.05E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.17|P-value:2.07E-5 ID:SGTC_1604|Compound:st002052|FD-Score:4.05|P-value:3.41E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.86|P-value:7.34E-5 ID:SGTC_1933|Compound:st074711|FD-Score:3.39|P-value:4.33E-4 ID:SGTC_1946|Compound:st076057|FD-Score:-3.20|P-value:7.49E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.31|P-value:5.80E-4 ID:SGTC_2144|Compound:5628481|FD-Score:5.17|P-value:1.84E-7 ID:SGTC_2316|Compound:5844804|FD-Score:-5.03|P-value:3.12E-7 ID:SGTC_2319|Compound:6033012|FD-Score:3.21|P-value:8.22E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-7.47|P-value:7.47E-14 ID:SGTC_2328|Compound:6201408|FD-Score:5.72|P-value:8.98E-9 ID:SGTC_2341|Compound:7987486|FD-Score:-3.57|P-value:2.00E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:7.22|P-value:5.96E-13 ID:SGTC_2705|Compound:st078069|FD-Score:3.33|P-value:5.43E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.47|P-value:3.27E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.57|P-value:2.02E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.09|P-value:2.49E-5 ID:SGTC_2783|Compound:7738165|FD-Score:6.61|P-value:3.83E-11 ID:SGTC_2788|Compound:5282734|FD-Score:-4.10|P-value:2.39E-5 ID:SGTC_3112|Compound:9123419|FD-Score:3.27|P-value:6.69E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.35|P-value:5.02E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.49|P-value:3.10E-4 ID:SGTC_106|Compound:0092-0003|FD-Score:3.36|P-value:4.82E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.47|P-value:3.23E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.40|P-value:3.74E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.26|P-value:6.93E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-3.61|P-value:1.71E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:-5.46|P-value:3.29E-8 ID:SGTC_1136|Compound:pergolide|FD-Score:3.56|P-value:2.36E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.17|P-value:9.35E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.25|P-value:7.12E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.48|P-value:3.10E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-4.86|P-value:7.32E-7 ID:SGTC_1377|Compound:2944-0135|FD-Score:-5.22|P-value:1.17E-7 ID:SGTC_1442|Compound:4112-3290|FD-Score:4.32|P-value:1.11E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.97|P-value:4.80E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.66|P-value:1.62E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.86|P-value:7.54E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.91|P-value:6.07E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:4.33|P-value:1.04E-5 ID:SGTC_1488|Compound:4013-0813|FD-Score:3.49|P-value:3.05E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.18|P-value:9.05E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.17|P-value:2.07E-5 ID:SGTC_1604|Compound:st002052|FD-Score:4.05|P-value:3.41E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.86|P-value:7.34E-5 ID:SGTC_1933|Compound:st074711|FD-Score:3.39|P-value:4.33E-4 ID:SGTC_1946|Compound:st076057|FD-Score:-3.20|P-value:7.49E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.31|P-value:5.80E-4 ID:SGTC_2144|Compound:5628481|FD-Score:5.17|P-value:1.84E-7 ID:SGTC_2316|Compound:5844804|FD-Score:-5.03|P-value:3.12E-7 ID:SGTC_2319|Compound:6033012|FD-Score:3.21|P-value:8.22E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-7.47|P-value:7.47E-14 ID:SGTC_2328|Compound:6201408|FD-Score:5.72|P-value:8.98E-9 ID:SGTC_2341|Compound:7987486|FD-Score:-3.57|P-value:2.00E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:7.22|P-value:5.96E-13 ID:SGTC_2705|Compound:st078069|FD-Score:3.33|P-value:5.43E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.47|P-value:3.27E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.57|P-value:2.02E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.09|P-value:2.49E-5 ID:SGTC_2783|Compound:7738165|FD-Score:6.61|P-value:3.83E-11 ID:SGTC_2788|Compound:5282734|FD-Score:-4.10|P-value:2.39E-5 ID:SGTC_3112|Compound:9123419|FD-Score:3.27|P-value:6.69E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.35|P-value:5.02E-4

Top fitness defect scores for YPL023C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2592 piperlongumine 7.22 83.3 µM 5.96E-13 TSC3-RPN4 5 2.65E-13
2 SGTC_2783 7738165 6.61 71.4 µM 3.83E-11 5 1.92E-11
3 SGTC_2328 6201408 5.72 200.0 µM 8.98E-9 3 5.26E-9
4 SGTC_2144 5628481 5.17 200.0 µM 1.84E-7 ERG2 7 1.18E-7
5 SGTC_1484 3473-2379 4.33 286.0 µM 1.04E-5 9 7.50E-6
6 SGTC_1442 4112-3290 4.32 3.0 µM 1.11E-5 6 7.98E-6
7 SGTC_1574 spiramycin 4.17 23.7 µM 2.07E-5 22 1.52E-5
8 SGTC_1604 st002052 4.05 72.4 µM 3.41E-5 TSC3-RPN4 25 2.54E-5
9 SGTC_1447 4239-0816 3.97 81.9 µM 4.80E-5 27 3.61E-5
10 SGTC_1472 k841-0093 3.91 193.0 µM 6.07E-5 18 4.60E-5
11 SGTC_1864 st057645 3.86 30.4 µM 7.34E-5 amide catabolism 5 5.59E-5
12 SGTC_1464 k208-0027 3.86 110.0 µM 7.54E-5 29 5.75E-5
13 SGTC_1454 4239-0054 3.66 42.6 µM 1.62E-4 44 1.27E-4
14 SGTC_1136 pergolide 3.56 1.1 µM 2.36E-4 56 1.86E-4
15 SGTC_1488 4013-0813 3.49 75.9 µM 3.05E-4 RPP1 & pyrimidine depletion 9 2.42E-4
16 SGTC_55 0767-0285 3.49 6.6 µM 3.10E-4 59 2.46E-4
17 SGTC_1369 1866-0067 3.48 208.0 µM 3.10E-4 RPP1 & pyrimidine depletion 56 2.47E-4
18 SGTC_240 5hr heat shock (37°C) + Streptovitacin 3.47 300.0 µM 3.23E-4 heat shock/prefoldin 56 2.57E-4
19 SGTC_2709 st078560 3.47 56.0 µM 3.27E-4 21 2.60E-4
20 SGTC_1933 st074711 3.39 15.9 µM 4.33E-4 69 3.48E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.096 2.51E-8 YKL006C-A SFT1 het Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
0.092 9.59E-8 YDR325W YCG1 het Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.088 3.26E-7 YDR445C_d YDR445C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.084 1.17E-6 YPR076W_d YPR076W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 1.75E-6 YPL001W HAT1 hom Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
0.082 1.88E-6 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.082 1.86E-6 YDR363W ESC2 hom Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
0.081 2.75E-6 YPL177C CUP9 hom Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.079 4.44E-6 YKL159C RCN1 hom Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
0.079 5.09E-6 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.078 6.23E-6 YBR076W ECM8 hom Non-essential protein of unknown function
0.076 1.14E-5 YOR139C_d YOR139C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
0.075 1.22E-5 YJL009W_d YJL009W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
0.075 1.50E-5 YLR116W MSL5 het Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
0.070 5.40E-5 YPR121W THI22 hom Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis