YPL032C / SVL3

Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL032C / SVL3

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:3.63|P-value:6.13E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:3.97|P-value:2.05E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.06|P-value:1.48E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:5.16|P-value:2.04E-6 ID:SGTC_206|Compound:4546-0025|FD-Score:-3.92|P-value:1.72E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:10.80|P-value:1.03E-22 ID:SGTC_395|Compound:anisomycin|FD-Score:3.60|P-value:6.77E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.13|P-value:1.17E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.49|P-value:9.47E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.42|P-value:6.39E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:-5.02|P-value:2.40E-6 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.84|P-value:2.22E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:4.87|P-value:6.80E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:7.99E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.61|P-value:6.61E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.59|P-value:6.92E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.51|P-value:8.89E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.39|P-value:9.70E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.64|P-value:6.01E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-4.37|P-value:3.33E-5 ID:SGTC_1648|Compound:st011736|FD-Score:3.83|P-value:3.24E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.59|P-value:6.92E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.74|P-value:4.32E-4 ID:SGTC_1774|Compound:st048371|FD-Score:4.44|P-value:3.68E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.48|P-value:7.32E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.80|P-value:1.07E-7 ID:SGTC_2061|Compound:5245884|FD-Score:-3.72|P-value:3.40E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.61|P-value:6.68E-4 ID:SGTC_2139|Compound:5578692|FD-Score:-4.44|P-value:2.55E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.65|P-value:5.72E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.55|P-value:7.85E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.50|P-value:9.13E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.56|P-value:7.75E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.14|P-value:1.14E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.68|P-value:3.90E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.47|P-value:7.55E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.65|P-value:4.21E-4 ID:SGTC_2703|Compound:st077852|FD-Score:6.30|P-value:8.72E-9 ID:SGTC_2717|Compound:st081029|FD-Score:4.64|P-value:1.73E-5 ID:SGTC_2795|Compound:7780010|FD-Score:5.06|P-value:3.15E-6 ID:SGTC_2854|Compound:9024559|FD-Score:6.80|P-value:5.76E-10 ID:SGTC_2868|Compound:9035608|FD-Score:4.27|P-value:6.95E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.72|P-value:4.63E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.05|P-value:1.51E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.41|P-value:6.65E-7 ID:SGTC_3196|Compound:9111891|FD-Score:3.81|P-value:3.49E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.63|P-value:6.13E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:3.97|P-value:2.05E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.06|P-value:1.48E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:5.16|P-value:2.04E-6 ID:SGTC_206|Compound:4546-0025|FD-Score:-3.92|P-value:1.72E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:10.80|P-value:1.03E-22 ID:SGTC_395|Compound:anisomycin|FD-Score:3.60|P-value:6.77E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.13|P-value:1.17E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.49|P-value:9.47E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.42|P-value:6.39E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:-5.02|P-value:2.40E-6 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.84|P-value:2.22E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:4.87|P-value:6.80E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:7.99E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.61|P-value:6.61E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.59|P-value:6.92E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.51|P-value:8.89E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.39|P-value:9.70E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.64|P-value:6.01E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-4.37|P-value:3.33E-5 ID:SGTC_1648|Compound:st011736|FD-Score:3.83|P-value:3.24E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.59|P-value:6.92E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.74|P-value:4.32E-4 ID:SGTC_1774|Compound:st048371|FD-Score:4.44|P-value:3.68E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.48|P-value:7.32E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.80|P-value:1.07E-7 ID:SGTC_2061|Compound:5245884|FD-Score:-3.72|P-value:3.40E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.61|P-value:6.68E-4 ID:SGTC_2139|Compound:5578692|FD-Score:-4.44|P-value:2.55E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.65|P-value:5.72E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.55|P-value:7.85E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.50|P-value:9.13E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.56|P-value:7.75E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.14|P-value:1.14E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.68|P-value:3.90E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.47|P-value:7.55E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.65|P-value:4.21E-4 ID:SGTC_2703|Compound:st077852|FD-Score:6.30|P-value:8.72E-9 ID:SGTC_2717|Compound:st081029|FD-Score:4.64|P-value:1.73E-5 ID:SGTC_2795|Compound:7780010|FD-Score:5.06|P-value:3.15E-6 ID:SGTC_2854|Compound:9024559|FD-Score:6.80|P-value:5.76E-10 ID:SGTC_2868|Compound:9035608|FD-Score:4.27|P-value:6.95E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.72|P-value:4.63E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.05|P-value:1.51E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.41|P-value:6.65E-7 ID:SGTC_3196|Compound:9111891|FD-Score:3.81|P-value:3.49E-4

Top fitness defect scores for YPL032C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_351 caspofungin 10.80 25.0 nM 1.03E-22 cell wall 6 1.06E-27
2 SGTC_2854 9024559 6.80 71.4 µM 5.76E-10 3 5.35E-12
3 SGTC_2703 st077852 6.30 53.4 µM 8.72E-9 2 1.52E-10
4 SGTC_2016 5121758 5.80 140.0 µM 1.07E-7 redox potentiating 13 3.33E-9
5 SGTC_453 enantio-paf c16 5.42 10.0 µM 6.39E-7 71 3.01E-8
6 SGTC_2948 9070189 5.41 9.4 µM 6.65E-7 plasma membrane duress 26 3.16E-8
7 SGTC_151 3013-0144 5.16 2.3 µM 2.04E-6 iron homeostasis 9 1.26E-7
8 SGTC_2795 7780010 5.06 54.5 µM 3.15E-6 8 2.15E-7
9 SGTC_783 0848-0141 4.87 92.7 µM 6.80E-6 RSC & ERG11 8 5.53E-7
10 SGTC_2717 st081029 4.64 62.3 µM 1.73E-5 9 1.75E-6
11 SGTC_1774 st048371 4.44 48.5 µM 3.68E-5 RNA processing & uracil transport 8 4.42E-6
12 SGTC_2868 9035608 4.27 32.5 µM 6.95E-5 13 9.66E-6
13 SGTC_2299 7976368 4.14 114.9 µM 1.14E-4 24 1.77E-5
14 SGTC_404 fumagillin 4.13 50.0 µM 1.17E-4 16 1.83E-5
15 SGTC_138 0850-0259 4.06 40.9 µM 1.48E-4 cell wall 193 2.44E-5
16 SGTC_2924 9010168 4.05 62.8 µM 1.51E-4 cell wall signaling 36 2.51E-5
17 SGTC_29 0299-0004 3.97 13.0 µM 2.05E-4 28 3.64E-5
18 SGTC_1648 st011736 3.83 69.8 µM 3.24E-4 PDR1 17 6.39E-5
19 SGTC_3196 9111891 3.81 49.5 µM 3.49E-4 mitochondrial processes 20 7.01E-5
20 SGTC_1740 st038286 3.74 13.2 µM 4.32E-4 32 9.12E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 2.34E-19 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.141 2.14E-16 YGL027C CWH41 hom Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.137 1.91E-15 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.127 1.39E-13 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.127 1.49E-13 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.127 1.94E-13 YCL021W-A_p YCL021W-A_p hom Putative protein of unknown function
0.123 9.87E-13 YNL283C WSC2 hom Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
0.123 1.01E-12 YMR273C ZDS1 hom Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
0.123 1.10E-12 YLR319C BUD6 hom Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
0.115 2.62E-11 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.111 1.16E-10 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.108 3.43E-10 YHR204W MNL1 hom Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation
0.106 7.00E-10 YMR061W RNA14 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.105 9.51E-10 YNL079C TPM1 hom Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
0.105 1.20E-9 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication