YPL040C / ISM1

Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL040C / ISM1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.75|P-value:1.95E-5 ID:SGTC_19|Compound:0279-0214|FD-Score:4.66|P-value:2.69E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.72|P-value:3.91E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:4.95|P-value:9.18E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.27|P-value:1.07E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:5.23|P-value:3.12E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:3.88|P-value:3.77E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.92|P-value:5.27E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:16.10|P-value:2.66E-43 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.18|P-value:2.40E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:12.30|P-value:2.35E-26 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.50|P-value:1.32E-19 ID:SGTC_310|Compound:1273-0058|FD-Score:12.90|P-value:1.66E-28 ID:SGTC_314|Compound:1671-0008|FD-Score:3.92|P-value:3.30E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-5.82|P-value:4.91E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:3.79|P-value:4.95E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.82|P-value:2.68E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.89|P-value:3.65E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.78|P-value:5.07E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.03|P-value:3.79E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-4.18|P-value:2.35E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:4.11|P-value:1.81E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.27|P-value:1.06E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:6.98|P-value:9.41E-10 ID:SGTC_832|Compound:4083-0019|FD-Score:5.40|P-value:1.52E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:4.06|P-value:2.12E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:4.99|P-value:8.05E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:5.34|P-value:1.95E-6 ID:SGTC_978|Compound:1416-0512|FD-Score:-4.72|P-value:3.85E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.67|P-value:6.99E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.92|P-value:3.35E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-4.50|P-value:8.34E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:-3.77|P-value:8.18E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.95|P-value:3.01E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:4.12|P-value:1.78E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.63|P-value:7.89E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-4.95|P-value:1.65E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.21|P-value:1.31E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.84|P-value:4.29E-4 ID:SGTC_1623|Compound:st003709|FD-Score:8.11|P-value:1.52E-12 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.67|P-value:7.00E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-6.33|P-value:5.08E-8 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.90|P-value:2.02E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.63|P-value:1.18E-8 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.77|P-value:5.27E-4 ID:SGTC_1961|Compound:st076550|FD-Score:4.29|P-value:1.01E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.22|P-value:2.08E-4 ID:SGTC_2041|Compound:5107442|FD-Score:4.09|P-value:1.91E-4 ID:SGTC_2045|Compound:5106652|FD-Score:4.16|P-value:1.54E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-4.33|P-value:1.47E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.43|P-value:6.19E-5 ID:SGTC_2078|Compound:5212016|FD-Score:5.73|P-value:3.77E-7 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:6.32|P-value:2.48E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.71|P-value:9.75E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:9.44|P-value:2.60E-16 ID:SGTC_2254|Compound:7938975|FD-Score:5.80|P-value:2.74E-7 ID:SGTC_2260|Compound:7964312|FD-Score:3.67|P-value:7.15E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.62|P-value:8.19E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.15|P-value:1.57E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.66|P-value:2.71E-5 ID:SGTC_2490|Compound:carminic acid|FD-Score:-3.85|P-value:6.44E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.91|P-value:1.08E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.48|P-value:8.85E-5 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.90|P-value:3.51E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.77|P-value:1.85E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-5.23|P-value:5.66E-6 ID:SGTC_2804|Compound:7954240|FD-Score:-4.01|P-value:3.99E-4 ID:SGTC_2825|Compound:7998094|FD-Score:4.91|P-value:1.08E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-4.51|P-value:8.02E-5 ID:SGTC_3303|Compound:9125618|FD-Score:-9.41|P-value:9.19E-16 ID:SGTC_10|Compound:0279-0212|FD-Score:4.75|P-value:1.95E-5 ID:SGTC_19|Compound:0279-0214|FD-Score:4.66|P-value:2.69E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.72|P-value:3.91E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:4.95|P-value:9.18E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.27|P-value:1.07E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:5.23|P-value:3.12E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:3.88|P-value:3.77E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.92|P-value:5.27E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:16.10|P-value:2.66E-43 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.18|P-value:2.40E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:12.30|P-value:2.35E-26 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.50|P-value:1.32E-19 ID:SGTC_310|Compound:1273-0058|FD-Score:12.90|P-value:1.66E-28 ID:SGTC_314|Compound:1671-0008|FD-Score:3.92|P-value:3.30E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-5.82|P-value:4.91E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:3.79|P-value:4.95E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.82|P-value:2.68E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.89|P-value:3.65E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.78|P-value:5.07E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.03|P-value:3.79E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-4.18|P-value:2.35E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:4.11|P-value:1.81E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.27|P-value:1.06E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:6.98|P-value:9.41E-10 ID:SGTC_832|Compound:4083-0019|FD-Score:5.40|P-value:1.52E-6 ID:SGTC_834|Compound:0312-0008|FD-Score:4.06|P-value:2.12E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:4.99|P-value:8.05E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:5.34|P-value:1.95E-6 ID:SGTC_978|Compound:1416-0512|FD-Score:-4.72|P-value:3.85E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.67|P-value:6.99E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.92|P-value:3.35E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-4.50|P-value:8.34E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:-3.77|P-value:8.18E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.95|P-value:3.01E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:4.12|P-value:1.78E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.63|P-value:7.89E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-4.95|P-value:1.65E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.21|P-value:1.31E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.84|P-value:4.29E-4 ID:SGTC_1623|Compound:st003709|FD-Score:8.11|P-value:1.52E-12 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.67|P-value:7.00E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-6.33|P-value:5.08E-8 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.90|P-value:2.02E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.63|P-value:1.18E-8 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.77|P-value:5.27E-4 ID:SGTC_1961|Compound:st076550|FD-Score:4.29|P-value:1.01E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.22|P-value:2.08E-4 ID:SGTC_2041|Compound:5107442|FD-Score:4.09|P-value:1.91E-4 ID:SGTC_2045|Compound:5106652|FD-Score:4.16|P-value:1.54E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-4.33|P-value:1.47E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.43|P-value:6.19E-5 ID:SGTC_2078|Compound:5212016|FD-Score:5.73|P-value:3.77E-7 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:6.32|P-value:2.48E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.71|P-value:9.75E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:9.44|P-value:2.60E-16 ID:SGTC_2254|Compound:7938975|FD-Score:5.80|P-value:2.74E-7 ID:SGTC_2260|Compound:7964312|FD-Score:3.67|P-value:7.15E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.62|P-value:8.19E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.15|P-value:1.57E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.66|P-value:2.71E-5 ID:SGTC_2490|Compound:carminic acid|FD-Score:-3.85|P-value:6.44E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.91|P-value:1.08E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.48|P-value:8.85E-5 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.90|P-value:3.51E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.77|P-value:1.85E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-5.23|P-value:5.66E-6 ID:SGTC_2804|Compound:7954240|FD-Score:-4.01|P-value:3.99E-4 ID:SGTC_2825|Compound:7998094|FD-Score:4.91|P-value:1.08E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-4.51|P-value:8.02E-5 ID:SGTC_3303|Compound:9125618|FD-Score:-9.41|P-value:9.19E-16

Top fitness defect scores for YPL040C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_173 1273-0061 16.10 2.7 µM 2.66E-43 mitochondrial stress 7 2.41E-58
2 SGTC_310 1273-0058 12.90 1.1 µM 1.66E-28 mitochondrial stress 18 4.25E-38
3 SGTC_236 1509-0011 12.30 5.3 µM 2.35E-26 144 3.70E-35
4 SGTC_275 trichlorophene 10.50 5.7 µM 1.32E-19 mitochondrial stress 39 6.03E-26
5 SGTC_2250 alverine citrate 9.44 130.0 µM 2.60E-16 11 1.87E-21
6 SGTC_1623 st003709 8.11 20.3 µM 1.52E-12 calcium & mitochondrial duress 6 2.49E-16
7 SGTC_831 k061-1017 6.98 217.0 µM 9.41E-10 translation 5 1.52E-12
8 SGTC_2232 chlorophetanol 6.32 41.5 µM 2.48E-8 TRP & mitochondrial translation 37 1.27E-10
9 SGTC_2254 7938975 5.80 95.2 µM 2.74E-7 23 3.26E-9
10 SGTC_2078 5212016 5.73 200.0 µM 3.77E-7 60S ribosome export 2 5.01E-9
11 SGTC_28 pentachloropyridine N-oxide 5.72 1.6 µM 3.91E-7 TSC3-RPN4 19 5.25E-9
12 SGTC_832 4083-0019 5.40 164.0 µM 1.52E-6 translation 28 3.27E-8
13 SGTC_911 3456-4043 5.34 236.0 µM 1.95E-6 mitochondrial processes 8 4.57E-8
14 SGTC_70 0141-0289 5.23 43.3 µM 3.12E-6 mitochondrial stress 108 8.59E-8
15 SGTC_908 3448-7491 4.99 47.7 µM 8.05E-6 mitochondrial stress 78 3.07E-7
16 SGTC_63 0099-0242 4.95 26.7 µM 9.18E-6 mitochondrial stress 59 3.66E-7
17 SGTC_2533 agelasine 4.91 13.7 µM 1.08E-5 ERG2 26 4.56E-7
18 SGTC_2825 7998094 4.91 58.4 µM 1.08E-5 fatty acid desaturase (OLE1) 15 4.57E-7
19 SGTC_2748 diazoxide 4.77 86.7 µM 1.85E-5 2 9.35E-7
20 SGTC_10 0279-0212 4.75 42.6 µM 1.95E-5 TRP & mitochondrial translation 74 1.00E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.534 3.09E-245 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.529 8.97E-242 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.520 4.82E-232 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.501 1.60E-212 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.497 6.44E-209 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.496 1.13E-207 YNL081C SWS2 hom Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
0.488 9.74E-201 YDR296W MHR1 hom Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.478 7.53E-191 YOL095C HMI1 hom Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.475 2.15E-188 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.474 2.26E-187 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.470 3.01E-184 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.466 2.56E-180 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.460 1.45E-175 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.459 1.82E-174 YMR293C HER2 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
0.459 2.39E-174 YMR257C PET111 hom Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane