YPL043W / NOP4

Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL043W / NOP4

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:-4.26|P-value:1.23E-6 ID:SGTC_38|Compound:0929-0051|FD-Score:3.64|P-value:3.18E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.02|P-value:4.53E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.84|P-value:1.23E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:5.28|P-value:3.04E-9 ID:SGTC_99|Compound:0109-0120|FD-Score:3.77|P-value:1.72E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.74|P-value:1.95E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.27|P-value:1.48E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.72|P-value:1.55E-10 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.12|P-value:2.99E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:12.20|P-value:1.06E-41 ID:SGTC_431|Compound:k089-0097|FD-Score:4.58|P-value:2.38E-7 ID:SGTC_526|Compound:4469-0568|FD-Score:5.05|P-value:1.41E-8 ID:SGTC_538|Compound:2922-0838|FD-Score:3.68|P-value:2.66E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.08|P-value:3.60E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.05|P-value:4.00E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.38|P-value:1.01E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:9.02|P-value:1.52E-23 ID:SGTC_616|Compound:4092-0450|FD-Score:4.33|P-value:9.60E-7 ID:SGTC_655|Compound:k060-0012|FD-Score:2.93|P-value:6.54E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-2.94|P-value:5.78E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.02|P-value:4.60E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.15|P-value:2.50E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:5.36|P-value:1.79E-9 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.00|P-value:1.92E-8 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.52|P-value:5.05E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.08|P-value:3.58E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.91|P-value:6.86E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.14|P-value:2.52E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.08|P-value:3.33E-4 ID:SGTC_1676|Compound:st018489|FD-Score:2.89|P-value:7.38E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.42|P-value:8.54E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:6.12|P-value:8.16E-12 ID:SGTC_1719|Compound:st032227|FD-Score:-2.95|P-value:5.45E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.21|P-value:1.91E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.99|P-value:4.60E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.16|P-value:2.61E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-3.70|P-value:2.15E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.54|P-value:4.92E-5 ID:SGTC_1950|Compound:st077106|FD-Score:3.38|P-value:9.99E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.14|P-value:2.67E-6 ID:SGTC_1956|Compound:st077085|FD-Score:3.18|P-value:2.41E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-2.80|P-value:9.59E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.54|P-value:4.61E-5 ID:SGTC_2003|Compound:4023581|FD-Score:-3.32|P-value:1.21E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.48|P-value:6.66E-5 ID:SGTC_2164|Compound:5685496|FD-Score:-3.31|P-value:1.24E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2192|Compound:5915882|FD-Score:3.10|P-value:3.31E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.82|P-value:1.18E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.19|P-value:2.31E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.99|P-value:5.60E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:5.34|P-value:2.02E-9 ID:SGTC_2692|Compound:st077426|FD-Score:3.13|P-value:2.90E-4 ID:SGTC_2796|Compound:7949634|FD-Score:2.91|P-value:6.96E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.22|P-value:2.00E-4 ID:SGTC_2808|Compound:7946468|FD-Score:2.94|P-value:6.19E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.43|P-value:8.00E-5 ID:SGTC_2825|Compound:7998094|FD-Score:2.85|P-value:8.68E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.15|P-value:2.65E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.09|P-value:3.45E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.09|P-value:3.13E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.84|P-value:9.06E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.62|P-value:3.14E-5 ID:SGTC_3296|Compound:9122258|FD-Score:-3.34|P-value:1.09E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-3.12|P-value:2.79E-4 ID:SGTC_3322|Compound:9139058|FD-Score:-3.08|P-value:3.30E-4 ID:SGTC_3330|Compound:9141424|FD-Score:4.83|P-value:5.22E-8 ID:SGTC_3351|Compound:9150312|FD-Score:3.68|P-value:2.59E-5 ID:SGTC_37|Compound:0566-0070|FD-Score:-4.26|P-value:1.23E-6 ID:SGTC_38|Compound:0929-0051|FD-Score:3.64|P-value:3.18E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.02|P-value:4.53E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.84|P-value:1.23E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:5.28|P-value:3.04E-9 ID:SGTC_99|Compound:0109-0120|FD-Score:3.77|P-value:1.72E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.74|P-value:1.95E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.27|P-value:1.48E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.72|P-value:1.55E-10 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.12|P-value:2.99E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:12.20|P-value:1.06E-41 ID:SGTC_431|Compound:k089-0097|FD-Score:4.58|P-value:2.38E-7 ID:SGTC_526|Compound:4469-0568|FD-Score:5.05|P-value:1.41E-8 ID:SGTC_538|Compound:2922-0838|FD-Score:3.68|P-value:2.66E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.08|P-value:3.60E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.05|P-value:4.00E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.38|P-value:1.01E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:9.02|P-value:1.52E-23 ID:SGTC_616|Compound:4092-0450|FD-Score:4.33|P-value:9.60E-7 ID:SGTC_655|Compound:k060-0012|FD-Score:2.93|P-value:6.54E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-2.94|P-value:5.78E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.02|P-value:4.60E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.15|P-value:2.50E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:5.36|P-value:1.79E-9 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.00|P-value:1.92E-8 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.52|P-value:5.05E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.08|P-value:3.58E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.91|P-value:6.86E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.14|P-value:2.52E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.08|P-value:3.33E-4 ID:SGTC_1676|Compound:st018489|FD-Score:2.89|P-value:7.38E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.42|P-value:8.54E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:6.12|P-value:8.16E-12 ID:SGTC_1719|Compound:st032227|FD-Score:-2.95|P-value:5.45E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.21|P-value:1.91E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.99|P-value:4.60E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.16|P-value:2.61E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-3.70|P-value:2.15E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.54|P-value:4.92E-5 ID:SGTC_1950|Compound:st077106|FD-Score:3.38|P-value:9.99E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.14|P-value:2.67E-6 ID:SGTC_1956|Compound:st077085|FD-Score:3.18|P-value:2.41E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-2.80|P-value:9.59E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.54|P-value:4.61E-5 ID:SGTC_2003|Compound:4023581|FD-Score:-3.32|P-value:1.21E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.48|P-value:6.66E-5 ID:SGTC_2164|Compound:5685496|FD-Score:-3.31|P-value:1.24E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2192|Compound:5915882|FD-Score:3.10|P-value:3.31E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.82|P-value:1.18E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.19|P-value:2.31E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.99|P-value:5.60E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:5.34|P-value:2.02E-9 ID:SGTC_2692|Compound:st077426|FD-Score:3.13|P-value:2.90E-4 ID:SGTC_2796|Compound:7949634|FD-Score:2.91|P-value:6.96E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.22|P-value:2.00E-4 ID:SGTC_2808|Compound:7946468|FD-Score:2.94|P-value:6.19E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.43|P-value:8.00E-5 ID:SGTC_2825|Compound:7998094|FD-Score:2.85|P-value:8.68E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.15|P-value:2.65E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.09|P-value:3.45E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.09|P-value:3.13E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.84|P-value:9.06E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.62|P-value:3.14E-5 ID:SGTC_3296|Compound:9122258|FD-Score:-3.34|P-value:1.09E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-3.12|P-value:2.79E-4 ID:SGTC_3322|Compound:9139058|FD-Score:-3.08|P-value:3.30E-4 ID:SGTC_3330|Compound:9141424|FD-Score:4.83|P-value:5.22E-8 ID:SGTC_3351|Compound:9150312|FD-Score:3.68|P-value:2.59E-5

Top fitness defect scores for YPL043W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_423 5-fluorouracil 12.20 457.0 µM 1.06E-41 exosome 3 1.05E-34 2.32
2 SGTC_610 4215-0184 9.02 155.0 µM 1.52E-23 exosome 4 9.73E-20 0.22
3 SGTC_1698 5-fluorocytosine 6.12 1.1 µM 8.16E-12 exosome 8 4.72E-10 0.11
4 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 5.72 4.6 µM 1.55E-10 heat shock/prefoldin 10 5.39E-9 0.04
5 SGTC_1074 carmofur 5.36 3.8 µM 1.79E-9 exosome 17 4.09E-8 0.02
6 SGTC_2689 tacrolimus 5.34 100.0 µM 2.02E-9 5 4.52E-8 0.43
7 SGTC_44 0929-0055 5.28 61.2 µM 3.04E-9 3 6.34E-8 0.90
8 SGTC_526 4469-0568 5.05 170.0 µM 1.41E-8 azole & statin 5 2.26E-7 1.01
9 SGTC_1077 5-fluorocytosine 5.00 377.0 nM 1.92E-8 exosome 14 2.92E-7 0.61
10 SGTC_3330 9141424 4.83 71.4 µM 5.22E-8 amide catabolism 1 6.69E-7 0.71
11 SGTC_431 k089-0097 4.58 107.0 µM 2.38E-7 iron homeostasis 8 2.36E-6 0.03
12 SGTC_616 4092-0450 4.33 108.0 µM 9.60E-7 3 7.51E-6 0.63
13 SGTC_1953 st076800 4.14 12.1 µM 2.67E-6 15 1.76E-5 0.39
14 SGTC_2687 chenodiol 3.99 100.0 µM 5.60E-6 4 3.25E-5 0.03
15 SGTC_42 0133-0086 3.84 60.6 µM 1.23E-5 9 6.26E-5 0.01
16 SGTC_99 0109-0120 3.77 622.8 µM 1.72E-5 8 8.27E-5 0.14
17 SGTC_118 0083-0097 3.74 135.3 µM 1.95E-5 translation 24 9.20E-5 0.00
18 SGTC_3351 9150312 3.68 21.6 µM 2.59E-5 cell wall 10 1.16E-4 0.02
19 SGTC_538 2922-0838 3.68 53.3 µM 2.66E-5 Golgi 7 1.19E-4 0.28
20 SGTC_38 0929-0051 3.64 58.3 µM 3.18E-5 6 1.38E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.464 9.46E-179 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.388 2.72E-121 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.388 4.11E-121 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.387 5.60E-120 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.341 9.12E-92 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.334 2.78E-88 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.331 9.68E-87 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.327 2.86E-84 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.285 9.96E-64 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.254 1.48E-50 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.210 6.37E-35 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.203 1.38E-32 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.196 2.46E-30 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.188 5.23E-28 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.179 1.48E-25 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)