YPL076W / GPI2

Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL076W / GPI2

Click on Significant Values for Screen Details ID:SGTC_155|Compound:k072-0202|FD-Score:-3.53|P-value:2.30E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.22|P-value:8.49E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:6.75|P-value:1.85E-11 ID:SGTC_511|Compound:blebbistatin|FD-Score:-3.24|P-value:6.51E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.39|P-value:3.78E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.38|P-value:4.77E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.26|P-value:7.28E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-5.11|P-value:2.12E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-4.41|P-value:6.30E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.52|P-value:2.98E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:5.46|P-value:4.54E-8 ID:SGTC_1246|Compound:0422-0085|FD-Score:-4.01|P-value:3.56E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.42|P-value:4.22E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:-3.72|P-value:1.13E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.27|P-value:7.10E-4 ID:SGTC_1746|Compound:st038117|FD-Score:-3.41|P-value:3.51E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.50|P-value:3.13E-4 ID:SGTC_1919|Compound:st067113|FD-Score:4.20|P-value:2.04E-5 ID:SGTC_1973|Compound:st070275|FD-Score:-3.39|P-value:3.83E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.72|P-value:1.11E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-6.49|P-value:7.14E-11 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:5.62|P-value:1.84E-8 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.26|P-value:7.28E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.29|P-value:6.72E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-4.24|P-value:1.34E-5 ID:SGTC_2973|Compound:9094665|FD-Score:-3.23|P-value:6.76E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.53|P-value:2.30E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.22|P-value:8.49E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:6.75|P-value:1.85E-11 ID:SGTC_511|Compound:blebbistatin|FD-Score:-3.24|P-value:6.51E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.39|P-value:3.78E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.38|P-value:4.77E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.26|P-value:7.28E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-5.11|P-value:2.12E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-4.41|P-value:6.30E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.52|P-value:2.98E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:5.46|P-value:4.54E-8 ID:SGTC_1246|Compound:0422-0085|FD-Score:-4.01|P-value:3.56E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.42|P-value:4.22E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:-3.72|P-value:1.13E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.27|P-value:7.10E-4 ID:SGTC_1746|Compound:st038117|FD-Score:-3.41|P-value:3.51E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.50|P-value:3.13E-4 ID:SGTC_1919|Compound:st067113|FD-Score:4.20|P-value:2.04E-5 ID:SGTC_1973|Compound:st070275|FD-Score:-3.39|P-value:3.83E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.72|P-value:1.11E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-6.49|P-value:7.14E-11 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:5.62|P-value:1.84E-8 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.26|P-value:7.28E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.29|P-value:6.72E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-4.24|P-value:1.34E-5 ID:SGTC_2973|Compound:9094665|FD-Score:-3.23|P-value:6.76E-4

Top fitness defect scores for YPL076W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_502 l-744,832 6.75 89.3 µM 1.85E-11 calcium & mitochondrial duress 2 7.43E-12 1.66
2 SGTC_2502 avocadyne acetate 5.62 18.6 µM 1.84E-8 3 9.46E-9 0.86
3 SGTC_1189 1431-2119 5.46 7.3 µM 4.54E-8 5 2.41E-8 0.60
4 SGTC_1919 st067113 4.20 52.3 µM 2.04E-5 TSC3-RPN4 8 1.36E-5 0.04
5 SGTC_1734 st037168 3.69 69.4 µM 1.57E-4 7 1.12E-4 0.24
6 SGTC_1072 oxiconazole 3.52 14.9 nM 2.98E-4 ergosterol depletion effects on membrane 26 2.18E-4 0.10
7 SGTC_1848 st056003 3.50 16.4 µM 3.13E-4 6 2.30E-4 0.06
8 SGTC_1428 4049-0204 3.42 61.7 µM 4.22E-4 4 3.13E-4 0.06
9 SGTC_674 0498-0004 3.38 451.0 µM 4.77E-4 heme requiring 32 3.56E-4 0.02
10 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 3.29 70.0 µM 6.72E-4 redox potentiating 15 5.08E-4 0.34
11 SGTC_1738 st035555 3.27 74.0 µM 7.10E-4 TSC3-RPN4 12 5.37E-4 0.05
12 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 3.26 54.6 µM 7.28E-4 RPP1 & pyrimidine depletion 19 5.52E-4 0.19
13 SGTC_922 1866-0035 3.26 480.0 µM 7.28E-4 excess fatty acid 17 5.52E-4 0.03
14 SGTC_390 dec-rvkr-cmk 3.22 16.7 µM 8.49E-4 calcium & mitochondrial duress 18 6.47E-4 0.12
15 SGTC_1766 st045414 3.06 44.8 µM 0.00141 RPP1 & pyrimidine depletion 22 0.00110 0.03
16 SGTC_817 1391-0715 3.01 46.0 µM 0.00169 19 0.00132 0.01
17 SGTC_2853 9015042 2.99 58.4 µM 0.00176 14 0.00138 0.12
18 SGTC_1249 0481-0015 2.96 10.2 µM 0.00193 17 0.00151 0.03
19 SGTC_400 oxethazaine 2.94 37.5 µM 0.00211 calcium & mitochondrial duress 15 0.00166 0.03
20 SGTC_948 3448-0295 2.92 75.3 µM 0.00222 17 0.00176 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.56E-13 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.107 5.75E-10 YCR032W BPH1 hom Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
0.098 1.17E-8 YOR061W CKA2 hom Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
0.098 1.52E-8 YJL131C AIM23 hom Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
0.081 2.55E-6 YNL059C ARP5 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.080 3.13E-6 YOL099C_d YOL099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
0.079 4.12E-6 YGR107W_d YGR107W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.078 5.74E-6 YOL128C YGK3 hom Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation
0.078 6.17E-6 YHR153C SPO16 hom Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
0.077 7.24E-6 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.076 9.38E-6 YNR062C_p YNR062C_p hom Putative membrane protein of unknown function
0.076 9.66E-6 YLR319C BUD6 hom Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
0.076 1.12E-5 YBR121C GRS1 het Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
0.075 1.51E-5 YGL152C_d YGL152C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
0.074 1.77E-5 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly