YPL082C / MOT1

Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL082C / MOT1

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:6.21|P-value:5.71E-12 ID:SGTC_422|Compound:cantharidin|FD-Score:-4.56|P-value:3.10E-7 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.16|P-value:2.78E-4 ID:SGTC_443|Compound:clozapine|FD-Score:5.66|P-value:2.97E-10 ID:SGTC_490|Compound:phenamil|FD-Score:2.96|P-value:6.03E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:3.48|P-value:7.24E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.51|P-value:6.13E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:3.25|P-value:1.89E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.01|P-value:4.99E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.63|P-value:3.68E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:3.45|P-value:8.26E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:3.23|P-value:2.09E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.22|P-value:2.13E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:-2.90|P-value:7.68E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.18|P-value:2.52E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.86|P-value:1.25E-5 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.38|P-value:1.09E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:2.95|P-value:6.27E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.35|P-value:1.26E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:2.86|P-value:8.83E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.11|P-value:3.37E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.27|P-value:1.73E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:2.99|P-value:5.43E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.88|P-value:8.12E-4 ID:SGTC_1735|Compound:st034310|FD-Score:4.31|P-value:1.23E-6 ID:SGTC_1809|Compound:st050081|FD-Score:2.93|P-value:6.84E-4 ID:SGTC_1902|Compound:st060212|FD-Score:2.88|P-value:8.27E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.12|P-value:3.33E-6 ID:SGTC_2043|Compound:5101342|FD-Score:-3.03|P-value:4.67E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-2.88|P-value:8.25E-4 ID:SGTC_2208|Compound:7283366|FD-Score:2.84|P-value:9.59E-4 ID:SGTC_2231|Compound:micatex|FD-Score:3.88|P-value:1.09E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.53|P-value:5.61E-5 ID:SGTC_2411|Compound:st077133|FD-Score:2.93|P-value:6.77E-4 ID:SGTC_2413|Compound:inermin|FD-Score:7.56|P-value:6.83E-17 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.79|P-value:8.02E-8 ID:SGTC_2471|Compound:5466956|FD-Score:-3.58|P-value:4.49E-5 ID:SGTC_2475|Compound:5812728|FD-Score:-3.30|P-value:1.54E-4 ID:SGTC_2485|Compound:5633444|FD-Score:-5.17|P-value:7.90E-9 ID:SGTC_2486|Compound:5283202|FD-Score:2.94|P-value:6.51E-4 ID:SGTC_2488|Compound:5268135|FD-Score:-4.98|P-value:2.58E-8 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.00|P-value:5.11E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.93|P-value:6.89E-4 ID:SGTC_2585|Compound:alliin|FD-Score:6.01|P-value:2.59E-11 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.67|P-value:3.03E-5 ID:SGTC_2683|Compound:piperine|FD-Score:3.33|P-value:1.36E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:-3.21|P-value:2.22E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:2.90|P-value:7.64E-4 ID:SGTC_2744|Compound:captopril|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.12|P-value:3.23E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.31|P-value:1.22E-6 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:2.95E-4 ID:SGTC_2818|Compound:7996756|FD-Score:-2.99|P-value:5.32E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.61|P-value:4.00E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-2.96|P-value:6.01E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.05|P-value:4.28E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.81|P-value:1.55E-5 ID:SGTC_2995|Compound:9067767|FD-Score:2.93|P-value:6.86E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.16|P-value:2.74E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.25|P-value:1.89E-4 ID:SGTC_3067|Compound:9117431|FD-Score:4.29|P-value:1.35E-6 ID:SGTC_3147|Compound:9097027|FD-Score:3.74|P-value:2.19E-5 ID:SGTC_3149|Compound:9097855|FD-Score:4.12|P-value:3.42E-6 ID:SGTC_3151|Compound:9099821|FD-Score:-5.99|P-value:2.95E-11 ID:SGTC_3152|Compound:9099253|FD-Score:-3.75|P-value:2.08E-5 ID:SGTC_3187|Compound:9109394|FD-Score:3.95|P-value:7.79E-6 ID:SGTC_3213|Compound:9129884|FD-Score:-4.18|P-value:2.45E-6 ID:SGTC_3215|Compound:9129942|FD-Score:3.24|P-value:1.97E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.09|P-value:3.86E-6 ID:SGTC_3220|Compound:9131269|FD-Score:-3.55|P-value:5.23E-5 ID:SGTC_3238|Compound:9133470|FD-Score:-2.96|P-value:6.08E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:6.21|P-value:5.71E-12 ID:SGTC_422|Compound:cantharidin|FD-Score:-4.56|P-value:3.10E-7 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.16|P-value:2.78E-4 ID:SGTC_443|Compound:clozapine|FD-Score:5.66|P-value:2.97E-10 ID:SGTC_490|Compound:phenamil|FD-Score:2.96|P-value:6.03E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:3.48|P-value:7.24E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.51|P-value:6.13E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:3.25|P-value:1.89E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.01|P-value:4.99E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.63|P-value:3.68E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:3.45|P-value:8.26E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:3.23|P-value:2.09E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.22|P-value:2.13E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:-2.90|P-value:7.68E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.18|P-value:2.52E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.86|P-value:1.25E-5 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.38|P-value:1.09E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:2.95|P-value:6.27E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.35|P-value:1.26E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:2.86|P-value:8.83E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.11|P-value:3.37E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.27|P-value:1.73E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:2.99|P-value:5.43E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.88|P-value:8.12E-4 ID:SGTC_1735|Compound:st034310|FD-Score:4.31|P-value:1.23E-6 ID:SGTC_1809|Compound:st050081|FD-Score:2.93|P-value:6.84E-4 ID:SGTC_1902|Compound:st060212|FD-Score:2.88|P-value:8.27E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.12|P-value:3.33E-6 ID:SGTC_2043|Compound:5101342|FD-Score:-3.03|P-value:4.67E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-2.88|P-value:8.25E-4 ID:SGTC_2208|Compound:7283366|FD-Score:2.84|P-value:9.59E-4 ID:SGTC_2231|Compound:micatex|FD-Score:3.88|P-value:1.09E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.53|P-value:5.61E-5 ID:SGTC_2411|Compound:st077133|FD-Score:2.93|P-value:6.77E-4 ID:SGTC_2413|Compound:inermin|FD-Score:7.56|P-value:6.83E-17 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.79|P-value:8.02E-8 ID:SGTC_2471|Compound:5466956|FD-Score:-3.58|P-value:4.49E-5 ID:SGTC_2475|Compound:5812728|FD-Score:-3.30|P-value:1.54E-4 ID:SGTC_2485|Compound:5633444|FD-Score:-5.17|P-value:7.90E-9 ID:SGTC_2486|Compound:5283202|FD-Score:2.94|P-value:6.51E-4 ID:SGTC_2488|Compound:5268135|FD-Score:-4.98|P-value:2.58E-8 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.00|P-value:5.11E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.93|P-value:6.89E-4 ID:SGTC_2585|Compound:alliin|FD-Score:6.01|P-value:2.59E-11 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.67|P-value:3.03E-5 ID:SGTC_2683|Compound:piperine|FD-Score:3.33|P-value:1.36E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:-3.21|P-value:2.22E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:2.90|P-value:7.64E-4 ID:SGTC_2744|Compound:captopril|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.12|P-value:3.23E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.31|P-value:1.22E-6 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:2.95E-4 ID:SGTC_2818|Compound:7996756|FD-Score:-2.99|P-value:5.32E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.61|P-value:4.00E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-2.96|P-value:6.01E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.05|P-value:4.28E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.81|P-value:1.55E-5 ID:SGTC_2995|Compound:9067767|FD-Score:2.93|P-value:6.86E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.16|P-value:2.74E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.25|P-value:1.89E-4 ID:SGTC_3067|Compound:9117431|FD-Score:4.29|P-value:1.35E-6 ID:SGTC_3147|Compound:9097027|FD-Score:3.74|P-value:2.19E-5 ID:SGTC_3149|Compound:9097855|FD-Score:4.12|P-value:3.42E-6 ID:SGTC_3151|Compound:9099821|FD-Score:-5.99|P-value:2.95E-11 ID:SGTC_3152|Compound:9099253|FD-Score:-3.75|P-value:2.08E-5 ID:SGTC_3187|Compound:9109394|FD-Score:3.95|P-value:7.79E-6 ID:SGTC_3213|Compound:9129884|FD-Score:-4.18|P-value:2.45E-6 ID:SGTC_3215|Compound:9129942|FD-Score:3.24|P-value:1.97E-4 ID:SGTC_3219|Compound:9131112|FD-Score:4.09|P-value:3.86E-6 ID:SGTC_3220|Compound:9131269|FD-Score:-3.55|P-value:5.23E-5 ID:SGTC_3238|Compound:9133470|FD-Score:-2.96|P-value:6.08E-4

Top fitness defect scores for YPL082C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2413 inermin 7.56 26.1 µM 6.83E-17 7 1.97E-14 1.12
2 SGTC_1 0258-0098 6.21 57.4 µM 5.71E-12 heme biosynthesis & mitochondrial translocase 5 2.67E-10 0.14
3 SGTC_2585 alliin 6.01 100.0 µM 2.59E-11 3 9.50E-10 0.72
4 SGTC_443 clozapine 5.66 122.0 µM 2.97E-10 NEO1-PIK1 2 7.37E-9 1.95
5 SGTC_2776 terbinafine 4.31 19.9 µM 1.22E-6 cell wall signaling 7 8.10E-6 0.22
6 SGTC_1735 st034310 4.31 56.8 µM 1.23E-6 Golgi 6 8.14E-6 0.03
7 SGTC_3067 9117431 4.29 49.5 µM 1.35E-6 4 8.86E-6 0.13
8 SGTC_1985 st071382 4.12 65.5 µM 3.33E-6 2 1.89E-5 0.27
9 SGTC_3149 9097855 4.12 49.5 µM 3.42E-6 Golgi 8 1.93E-5 0.03
10 SGTC_3219 9131112 4.09 49.5 µM 3.86E-6 Golgi 17 2.14E-5 0.02
11 SGTC_3187 9109394 3.95 49.5 µM 7.79E-6 Golgi 10 3.87E-5 0.08
12 SGTC_2231 micatex 3.88 6.8 µM 1.09E-5 8 5.15E-5 0.11
13 SGTC_2940 9060638 3.81 45.9 µM 1.55E-5 amide catabolism 7 6.91E-5 0.14
14 SGTC_3147 9097027 3.74 49.5 µM 2.19E-5 11 9.28E-5 0.08
15 SGTC_824 1304-0008 3.63 100.0 µM 3.68E-5 DNA damage response 10 1.44E-4 0.18
16 SGTC_2309 7664088 3.53 163.9 µM 5.61E-5 13 2.05E-4 0.01
17 SGTC_783 0848-0141 3.51 92.7 µM 6.13E-5 RSC & ERG11 21 2.21E-4 0.06
18 SGTC_607 4491-0986 3.48 66.8 µM 7.24E-5 heme requiring 8 2.55E-4 0.08
19 SGTC_827 3253-0474 3.45 693.0 µM 8.26E-5 10 2.85E-4 0.02
20 SGTC_1150 3909-7983 3.38 10.9 µM 1.09E-4 12 3.61E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.226 4.16E-40 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.167 2.09E-22 YMR096W SNZ1 hom Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins
0.141 2.25E-16 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.134 5.64E-15 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.126 2.12E-13 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.123 8.26E-13 YCL012C YCL012C hom Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
0.122 1.13E-12 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.118 7.45E-12 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.116 1.74E-11 YFL041W FET5 hom Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport
0.112 6.84E-11 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.105 9.21E-10 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.104 1.49E-9 YBL040C ERD2 het HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
0.104 1.58E-9 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.102 3.69E-9 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.102 3.81E-9 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane