YPL084W / BRO1

Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL084W / BRO1

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:4.67|P-value:2.15E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.47|P-value:3.20E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.53|P-value:2.59E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.28|P-value:6.36E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.11|P-value:2.50E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:6.26|P-value:3.42E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:5.13|P-value:2.24E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:8.24|P-value:2.41E-16 ID:SGTC_168|Compound:3970-0862|FD-Score:4.98|P-value:4.66E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.09|P-value:2.72E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:3.55|P-value:2.45E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.37|P-value:4.57E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.24|P-value:7.20E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.94|P-value:4.64E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.51|P-value:3.94E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:4.37|P-value:8.38E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.98|P-value:4.57E-5 ID:SGTC_493|Compound:zm226600|FD-Score:5.09|P-value:2.69E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:4.42|P-value:6.71E-6 ID:SGTC_579|Compound:4491-1009|FD-Score:3.28|P-value:6.25E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:4.75|P-value:1.44E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:5.39|P-value:5.49E-8 ID:SGTC_710|Compound:3448-4187|FD-Score:4.31|P-value:1.11E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:4.79|P-value:1.19E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:4.26|P-value:1.37E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-6.80|P-value:8.57E-12 ID:SGTC_925|Compound:2064-1272|FD-Score:4.43|P-value:6.58E-6 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.07|P-value:3.05E-7 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.73|P-value:1.23E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.42|P-value:6.73E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.29|P-value:6.13E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.26|P-value:6.88E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.69|P-value:1.41E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.50|P-value:2.89E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.84|P-value:7.90E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:3.59|P-value:2.06E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.32|P-value:5.58E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.30|P-value:3.22E-13 ID:SGTC_1750|Compound:st081859|FD-Score:4.39|P-value:7.90E-6 ID:SGTC_1800|Compound:st050079|FD-Score:5.63|P-value:1.52E-8 ID:SGTC_1801|Compound:st050089|FD-Score:4.27|P-value:1.35E-5 ID:SGTC_1803|Compound:st050068|FD-Score:5.28|P-value:1.01E-7 ID:SGTC_1825|Compound:st052143|FD-Score:4.33|P-value:1.03E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:6.60|P-value:4.03E-11 ID:SGTC_1889|Compound:st059080|FD-Score:3.53|P-value:2.62E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.51|P-value:2.81E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.45|P-value:5.84E-6 ID:SGTC_2151|Compound:5563267|FD-Score:3.57|P-value:2.25E-4 ID:SGTC_2199|Compound:6590217|FD-Score:3.39|P-value:4.27E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.76|P-value:9.80E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.51|P-value:2.83E-8 ID:SGTC_2364|Compound:9055074|FD-Score:3.41|P-value:3.97E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.07|P-value:3.18E-5 ID:SGTC_2430|Compound:5571206|FD-Score:3.90|P-value:6.17E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-4.19|P-value:1.69E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.57|P-value:1.97E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.25|P-value:6.91E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:4.09|P-value:2.88E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.21|P-value:8.12E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.37|P-value:1.71E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.68|P-value:7.01E-22 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.03|P-value:3.67E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:6.50|P-value:7.45E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.08|P-value:1.07E-9 ID:SGTC_2792|Compound:7535235|FD-Score:4.58|P-value:3.30E-6 ID:SGTC_2818|Compound:7996756|FD-Score:-3.46|P-value:2.96E-4 ID:SGTC_2829|Compound:7992743|FD-Score:5.80|P-value:5.63E-9 ID:SGTC_2832|Compound:7992762|FD-Score:-5.32|P-value:7.07E-8 ID:SGTC_2835|Compound:9006588|FD-Score:-4.06|P-value:2.92E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-3.75|P-value:1.00E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-3.93|P-value:4.95E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.78|P-value:1.02E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.19|P-value:8.61E-4 ID:SGTC_2921|Compound:7974240|FD-Score:4.22|P-value:1.63E-5 ID:SGTC_3237|Compound:9133362|FD-Score:3.16|P-value:9.36E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.14|P-value:9.35E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:4.67|P-value:2.15E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.47|P-value:3.20E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.53|P-value:2.59E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.28|P-value:6.36E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.11|P-value:2.50E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:6.26|P-value:3.42E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:5.13|P-value:2.24E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:8.24|P-value:2.41E-16 ID:SGTC_168|Compound:3970-0862|FD-Score:4.98|P-value:4.66E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.09|P-value:2.72E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:3.55|P-value:2.45E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.37|P-value:4.57E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.24|P-value:7.20E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.94|P-value:4.64E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.51|P-value:3.94E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:4.37|P-value:8.38E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.98|P-value:4.57E-5 ID:SGTC_493|Compound:zm226600|FD-Score:5.09|P-value:2.69E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:4.42|P-value:6.71E-6 ID:SGTC_579|Compound:4491-1009|FD-Score:3.28|P-value:6.25E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:4.75|P-value:1.44E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:5.39|P-value:5.49E-8 ID:SGTC_710|Compound:3448-4187|FD-Score:4.31|P-value:1.11E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:4.79|P-value:1.19E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:4.26|P-value:1.37E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-6.80|P-value:8.57E-12 ID:SGTC_925|Compound:2064-1272|FD-Score:4.43|P-value:6.58E-6 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.07|P-value:3.05E-7 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.73|P-value:1.23E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.42|P-value:6.73E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.29|P-value:6.13E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.26|P-value:6.88E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.69|P-value:1.41E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.50|P-value:2.89E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.84|P-value:7.90E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:3.59|P-value:2.06E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.32|P-value:5.58E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.30|P-value:3.22E-13 ID:SGTC_1750|Compound:st081859|FD-Score:4.39|P-value:7.90E-6 ID:SGTC_1800|Compound:st050079|FD-Score:5.63|P-value:1.52E-8 ID:SGTC_1801|Compound:st050089|FD-Score:4.27|P-value:1.35E-5 ID:SGTC_1803|Compound:st050068|FD-Score:5.28|P-value:1.01E-7 ID:SGTC_1825|Compound:st052143|FD-Score:4.33|P-value:1.03E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:6.60|P-value:4.03E-11 ID:SGTC_1889|Compound:st059080|FD-Score:3.53|P-value:2.62E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.51|P-value:2.81E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.45|P-value:5.84E-6 ID:SGTC_2151|Compound:5563267|FD-Score:3.57|P-value:2.25E-4 ID:SGTC_2199|Compound:6590217|FD-Score:3.39|P-value:4.27E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.76|P-value:9.80E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.51|P-value:2.83E-8 ID:SGTC_2364|Compound:9055074|FD-Score:3.41|P-value:3.97E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.07|P-value:3.18E-5 ID:SGTC_2430|Compound:5571206|FD-Score:3.90|P-value:6.17E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-4.19|P-value:1.69E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.57|P-value:1.97E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.25|P-value:6.91E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:4.09|P-value:2.88E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.21|P-value:8.12E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.37|P-value:1.71E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.68|P-value:7.01E-22 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.03|P-value:3.67E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:6.50|P-value:7.45E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.08|P-value:1.07E-9 ID:SGTC_2792|Compound:7535235|FD-Score:4.58|P-value:3.30E-6 ID:SGTC_2818|Compound:7996756|FD-Score:-3.46|P-value:2.96E-4 ID:SGTC_2829|Compound:7992743|FD-Score:5.80|P-value:5.63E-9 ID:SGTC_2832|Compound:7992762|FD-Score:-5.32|P-value:7.07E-8 ID:SGTC_2835|Compound:9006588|FD-Score:-4.06|P-value:2.92E-5 ID:SGTC_2836|Compound:9010338|FD-Score:-3.75|P-value:1.00E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-3.93|P-value:4.95E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.78|P-value:1.02E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.19|P-value:8.61E-4 ID:SGTC_2921|Compound:7974240|FD-Score:4.22|P-value:1.63E-5 ID:SGTC_3237|Compound:9133362|FD-Score:3.16|P-value:9.36E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.14|P-value:9.35E-4

Top fitness defect scores for YPL084W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 9.68 100.0 µM 7.01E-22 redox potentiating 72 1.84E-22
2 SGTC_159 3970-0959 8.24 50.0 µM 2.41E-16 11 8.95E-17
3 SGTC_1687 st020778 7.30 55.4 µM 3.22E-13 82 1.46E-13
4 SGTC_1844 rapamycin 6.60 4.0 nM 4.03E-11 78 2.08E-11
5 SGTC_2619 obliquin 6.50 87.9 µM 7.45E-11 cell wall 66 3.92E-11
6 SGTC_2567 aureobasidin a 6.37 60.0 nM 1.71E-10 39 9.21E-11
7 SGTC_149 3970-0822 6.26 46.1 µM 3.42E-10 tubulin folding & SWR complex 33 1.87E-10
8 SGTC_2679 rapamycin 6.08 1.0 nM 1.07E-9 74 6.07E-10
9 SGTC_2829 7992743 5.80 58.4 µM 5.63E-9 9 3.34E-9
10 SGTC_1800 st050079 5.63 29.1 µM 1.52E-8 NEO1-PIK1 49 9.24E-9
11 SGTC_2322 7413319 5.51 38.2 µM 2.83E-8 ERAD & cell cycle 74 1.75E-8
12 SGTC_686 1319-0111 5.39 510.0 µM 5.49E-8 60S ribosome export 4 3.47E-8
13 SGTC_1803 st050068 5.28 52.4 µM 1.01E-7 NEO1-PIK1 106 6.48E-8
14 SGTC_150 3970-0969 5.13 77.2 µM 2.24E-7 35 1.47E-7
15 SGTC_146 3448-8650 5.11 53.1 µM 2.50E-7 NEO1-PIK1 83 1.65E-7
16 SGTC_493 zm226600 5.09 134.0 µM 2.69E-7 mitochondrial processes 6 1.78E-7
17 SGTC_221 trifluoperazine 5.09 10.3 µM 2.72E-7 NEO1-PIK1 155 1.79E-7
18 SGTC_1027 k015-0025 5.07 8.7 µM 3.05E-7 12 2.02E-7
19 SGTC_168 3970-0862 4.98 530.5 µM 4.66E-7 70 3.13E-7
20 SGTC_805 3852-0218 4.79 479.0 µM 1.19E-6 ERAD & cell cycle 42 8.16E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.344 2.10E-93 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.309 6.00E-75 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.305 3.24E-73 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.301 4.78E-71 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.299 1.97E-70 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.277 4.50E-60 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.275 3.39E-59 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.260 6.41E-53 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.257 1.02E-51 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.252 9.27E-50 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.250 6.45E-49 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.249 1.64E-48 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.246 1.59E-47 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.240 3.39E-45 YCL008C STP22 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
0.233 1.63E-42 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin