YPL086C / ELP3

Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL086C / ELP3

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.60|P-value:5.39E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.96|P-value:3.68E-6 ID:SGTC_57|Compound:miconazole|FD-Score:-4.19|P-value:1.06E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.13|P-value:9.15E-5 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.75|P-value:4.66E-4 ID:SGTC_389|Compound:tpck|FD-Score:-3.53|P-value:9.20E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:6.75E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.16|P-value:2.45E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:6.46|P-value:2.75E-9 ID:SGTC_680|Compound:0417-1641|FD-Score:3.94|P-value:1.74E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:5.30|P-value:8.34E-7 ID:SGTC_686|Compound:1319-0111|FD-Score:-4.40|P-value:5.09E-5 ID:SGTC_694|Compound:1315-0438|FD-Score:-4.72|P-value:1.46E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:-4.09|P-value:1.54E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.77|P-value:7.84E-6 ID:SGTC_708|Compound:3448-5400|FD-Score:5.32|P-value:7.60E-7 ID:SGTC_710|Compound:3448-4187|FD-Score:-5.91|P-value:7.98E-8 ID:SGTC_785|Compound:1270-0111|FD-Score:4.31|P-value:4.66E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:-4.03|P-value:1.84E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:4.43|P-value:3.00E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.42|P-value:9.38E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.85|P-value:2.41E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:5.75|P-value:1.01E-7 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.45|P-value:8.64E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:4.32|P-value:4.59E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:5.15|P-value:1.58E-6 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.79|P-value:7.22E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:5.30|P-value:8.24E-7 ID:SGTC_1625|Compound:st003711|FD-Score:-4.86|P-value:8.66E-6 ID:SGTC_1632|Compound:st005650|FD-Score:4.15|P-value:8.57E-5 ID:SGTC_1636|Compound:st007707|FD-Score:-5.18|P-value:2.20E-6 ID:SGTC_1638|Compound:st008370|FD-Score:3.52|P-value:6.96E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.56|P-value:6.09E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.44|P-value:8.75E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-5.23|P-value:1.78E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.59|P-value:5.60E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:6.20|P-value:1.09E-8 ID:SGTC_1912|Compound:st060209|FD-Score:3.62|P-value:4.99E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.42|P-value:9.53E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.14|P-value:8.69E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.66|P-value:4.44E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.32|P-value:6.63E-5 ID:SGTC_2511|Compound:berberine|FD-Score:3.80|P-value:2.78E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.57|P-value:5.96E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.75|P-value:8.52E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.78|P-value:2.96E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:6.03|P-value:2.62E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.31|P-value:6.32E-9 ID:SGTC_2794|Compound:7785382|FD-Score:-4.46|P-value:4.10E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.51|P-value:9.95E-4 ID:SGTC_2954|Compound:9074818|FD-Score:4.90|P-value:4.60E-6 ID:SGTC_2961|Compound:9083290|FD-Score:3.78|P-value:3.00E-4 ID:SGTC_2963|Compound:9082507|FD-Score:9.11|P-value:1.17E-16 ID:SGTC_3052|Compound:9092717|FD-Score:4.19|P-value:7.30E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.77|P-value:3.12E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.50|P-value:7.30E-4 ID:SGTC_3181|Compound:9108277|FD-Score:4.57|P-value:1.76E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.68|P-value:4.16E-4 ID:SGTC_3341|Compound:9147222|FD-Score:-5.35|P-value:1.09E-6 ID:SGTC_20|Compound:0481-0013|FD-Score:3.60|P-value:5.39E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.96|P-value:3.68E-6 ID:SGTC_57|Compound:miconazole|FD-Score:-4.19|P-value:1.06E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.13|P-value:9.15E-5 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.75|P-value:4.66E-4 ID:SGTC_389|Compound:tpck|FD-Score:-3.53|P-value:9.20E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.32|P-value:6.75E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.16|P-value:2.45E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:6.46|P-value:2.75E-9 ID:SGTC_680|Compound:0417-1641|FD-Score:3.94|P-value:1.74E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:5.30|P-value:8.34E-7 ID:SGTC_686|Compound:1319-0111|FD-Score:-4.40|P-value:5.09E-5 ID:SGTC_694|Compound:1315-0438|FD-Score:-4.72|P-value:1.46E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:-4.09|P-value:1.54E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.77|P-value:7.84E-6 ID:SGTC_708|Compound:3448-5400|FD-Score:5.32|P-value:7.60E-7 ID:SGTC_710|Compound:3448-4187|FD-Score:-5.91|P-value:7.98E-8 ID:SGTC_785|Compound:1270-0111|FD-Score:4.31|P-value:4.66E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:-4.03|P-value:1.84E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:4.43|P-value:3.00E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.42|P-value:9.38E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.85|P-value:2.41E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:5.75|P-value:1.01E-7 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.45|P-value:8.64E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:4.32|P-value:4.59E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:5.15|P-value:1.58E-6 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.79|P-value:7.22E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:5.30|P-value:8.24E-7 ID:SGTC_1625|Compound:st003711|FD-Score:-4.86|P-value:8.66E-6 ID:SGTC_1632|Compound:st005650|FD-Score:4.15|P-value:8.57E-5 ID:SGTC_1636|Compound:st007707|FD-Score:-5.18|P-value:2.20E-6 ID:SGTC_1638|Compound:st008370|FD-Score:3.52|P-value:6.96E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.56|P-value:6.09E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.44|P-value:8.75E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-5.23|P-value:1.78E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.59|P-value:5.60E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:6.20|P-value:1.09E-8 ID:SGTC_1912|Compound:st060209|FD-Score:3.62|P-value:4.99E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.42|P-value:9.53E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.14|P-value:8.69E-5 ID:SGTC_2471|Compound:5466956|FD-Score:3.66|P-value:4.44E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.32|P-value:6.63E-5 ID:SGTC_2511|Compound:berberine|FD-Score:3.80|P-value:2.78E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.57|P-value:5.96E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.75|P-value:8.52E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.78|P-value:2.96E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:6.03|P-value:2.62E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.31|P-value:6.32E-9 ID:SGTC_2794|Compound:7785382|FD-Score:-4.46|P-value:4.10E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.51|P-value:9.95E-4 ID:SGTC_2954|Compound:9074818|FD-Score:4.90|P-value:4.60E-6 ID:SGTC_2961|Compound:9083290|FD-Score:3.78|P-value:3.00E-4 ID:SGTC_2963|Compound:9082507|FD-Score:9.11|P-value:1.17E-16 ID:SGTC_3052|Compound:9092717|FD-Score:4.19|P-value:7.30E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.77|P-value:3.12E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.50|P-value:7.30E-4 ID:SGTC_3181|Compound:9108277|FD-Score:4.57|P-value:1.76E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.68|P-value:4.16E-4 ID:SGTC_3341|Compound:9147222|FD-Score:-5.35|P-value:1.09E-6

Top fitness defect scores for YPL086C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2963 9082507 9.11 9.0 µM 1.17E-16 1 4.02E-20
2 SGTC_577 r071-0003 6.46 38.6 µM 2.75E-9 fatty acid desaturase (OLE1) 12 5.15E-11
3 SGTC_2679 rapamycin 6.31 1.0 nM 6.32E-9 69 1.44E-10
4 SGTC_1844 rapamycin 6.20 4.0 nM 1.09E-8 85 2.82E-10
5 SGTC_2630 antimycin a 6.03 100.0 µM 2.62E-8 mitochondrial processes 11 8.29E-10
6 SGTC_1213 1315-0166 5.75 115.0 µM 1.01E-7 11 4.36E-9
7 SGTC_708 3448-5400 5.32 146.0 µM 7.60E-7 13 5.20E-8
8 SGTC_1506 4226-2149 5.30 212.0 µM 8.24E-7 10 5.75E-8
9 SGTC_685 1278-0033 5.30 54.9 µM 8.34E-7 60S ribosome export 4 5.83E-8
10 SGTC_1274 0865-0114 5.15 173.0 µM 1.58E-6 7 1.28E-7
11 SGTC_37 0566-0070 4.96 1.6 µM 3.68E-6 12 3.61E-7
12 SGTC_2954 9074818 4.90 52.7 µM 4.60E-6 21 4.74E-7
13 SGTC_1380 2291-0730 4.79 164.0 µM 7.22E-6 10 8.24E-7
14 SGTC_701 4424-0219 4.77 185.0 µM 7.84E-6 calcium & mitochondrial duress 10 9.11E-7
15 SGTC_2538 3',7'-epoxycaryophyllan-6-one 4.75 100.0 µM 8.52E-6 13 1.01E-6
16 SGTC_3181 9108277 4.57 49.5 µM 1.76E-5 iron homeostasis 6 2.45E-6
17 SGTC_983 1491-0821 4.43 12.2 µM 3.00E-5 7 4.71E-6
18 SGTC_1268 0828-0275 4.32 2.5 µM 4.59E-5 16 7.91E-6
19 SGTC_785 1270-0111 4.31 65.3 µM 4.66E-5 17 8.07E-6
20 SGTC_3052 9092717 4.19 49.5 µM 7.30E-5 17 1.40E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 8.32E-28 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.173 5.42E-24 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.170 3.26E-23 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.145 3.59E-17 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.143 8.04E-17 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.142 1.25E-16 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.140 4.76E-16 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.136 3.06E-15 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.132 2.13E-14 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.122 1.43E-12 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.120 2.76E-12 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.120 3.64E-12 YOR069W VPS5 hom Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
0.118 5.77E-12 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.118 6.10E-12 YAL020C ATS1 hom Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
0.113 5.89E-11 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs