YPL096W / PNG1

Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL096W / PNG1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.41|P-value:4.57E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.68|P-value:1.73E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.66|P-value:1.43E-8 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.16|P-value:2.26E-5 ID:SGTC_235|Compound:0330-0087|FD-Score:3.30|P-value:6.68E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.66|P-value:1.85E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.25|P-value:1.73E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:4.67|P-value:2.73E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:5.45|P-value:5.41E-8 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.38|P-value:4.43E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.92|P-value:6.92E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:-4.47|P-value:5.77E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:4.61|P-value:3.49E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:6.29|P-value:4.22E-10 ID:SGTC_903|Compound:1134-0042|FD-Score:3.81|P-value:1.06E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.50|P-value:3.34E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.84|P-value:9.12E-5 ID:SGTC_1311|Compound:1190-0148|FD-Score:5.22|P-value:1.79E-7 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.67|P-value:1.79E-4 ID:SGTC_1625|Compound:st003711|FD-Score:5.25|P-value:1.58E-7 ID:SGTC_1632|Compound:st005650|FD-Score:5.18|P-value:2.20E-7 ID:SGTC_1690|Compound:st023502|FD-Score:4.99|P-value:5.75E-7 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.82|P-value:1.02E-4 ID:SGTC_1908|Compound:st060353|FD-Score:5.71|P-value:1.33E-8 ID:SGTC_2284|Compound:7945075|FD-Score:3.33|P-value:5.94E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-3.63|P-value:1.83E-4 ID:SGTC_2370|Compound:9071495|FD-Score:-3.16|P-value:9.65E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.59|P-value:2.37E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.79|P-value:1.13E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.38|P-value:5.05E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.28|P-value:1.32E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.20|P-value:9.24E-4 ID:SGTC_2974|Compound:9094440|FD-Score:4.89|P-value:9.56E-7 ID:SGTC_2984|Compound:9022109|FD-Score:3.32|P-value:6.11E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.41|P-value:4.57E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.68|P-value:1.73E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.66|P-value:1.43E-8 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.16|P-value:2.26E-5 ID:SGTC_235|Compound:0330-0087|FD-Score:3.30|P-value:6.68E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.66|P-value:1.85E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:4.25|P-value:1.73E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:4.67|P-value:2.73E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:5.45|P-value:5.41E-8 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.38|P-value:4.43E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.92|P-value:6.92E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:-4.47|P-value:5.77E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:4.61|P-value:3.49E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:6.29|P-value:4.22E-10 ID:SGTC_903|Compound:1134-0042|FD-Score:3.81|P-value:1.06E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.50|P-value:3.34E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.84|P-value:9.12E-5 ID:SGTC_1311|Compound:1190-0148|FD-Score:5.22|P-value:1.79E-7 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.67|P-value:1.79E-4 ID:SGTC_1625|Compound:st003711|FD-Score:5.25|P-value:1.58E-7 ID:SGTC_1632|Compound:st005650|FD-Score:5.18|P-value:2.20E-7 ID:SGTC_1690|Compound:st023502|FD-Score:4.99|P-value:5.75E-7 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.82|P-value:1.02E-4 ID:SGTC_1908|Compound:st060353|FD-Score:5.71|P-value:1.33E-8 ID:SGTC_2284|Compound:7945075|FD-Score:3.33|P-value:5.94E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-3.63|P-value:1.83E-4 ID:SGTC_2370|Compound:9071495|FD-Score:-3.16|P-value:9.65E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.59|P-value:2.37E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.79|P-value:1.13E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.38|P-value:5.05E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.28|P-value:1.32E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.20|P-value:9.24E-4 ID:SGTC_2974|Compound:9094440|FD-Score:4.89|P-value:9.56E-7 ID:SGTC_2984|Compound:9022109|FD-Score:3.32|P-value:6.11E-4

Top fitness defect scores for YPL096W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_792 4534-1957 6.29 155.0 µM 4.22E-10 8 1.55E-10
2 SGTC_1908 st060353 5.71 56.5 µM 1.33E-8 RPP1 & pyrimidine depletion 9 5.78E-9
3 SGTC_568 1193-0097 5.45 7.5 µM 5.41E-8 ERAD & cell cycle 71 2.50E-8
4 SGTC_1625 st003711 5.25 30.1 µM 1.58E-7 calcium & mitochondrial duress 15 7.72E-8
5 SGTC_1311 1190-0148 5.22 100.0 µM 1.79E-7 azole & statin 13 8.78E-8
6 SGTC_1632 st005650 5.18 90.0 µM 2.20E-7 6 1.09E-7
7 SGTC_1690 st023502 4.99 41.2 µM 5.75E-7 calcium & mitochondrial duress 4 2.98E-7
8 SGTC_2974 9094440 4.89 9.7 µM 9.56E-7 ergosterol biosynthesis 26 5.08E-7
9 SGTC_554 4483-2211 4.67 187.0 µM 2.73E-6 8 1.53E-6
10 SGTC_783 0848-0141 4.61 92.7 µM 3.49E-6 RSC & ERG11 9 1.97E-6
11 SGTC_302 1094-0063 4.25 18.9 µM 1.73E-5 6 1.06E-5
12 SGTC_776 4476-4378 3.92 112.0 µM 6.92E-5 ERG2 20 4.52E-5
13 SGTC_1156 4092-0367 3.84 4.9 µM 9.12E-5 25 6.03E-5
14 SGTC_1879 gemcitabine 3.82 100.0 µM 1.02E-4 18 6.80E-5
15 SGTC_903 1134-0042 3.81 114.0 µM 1.06E-4 22 7.05E-5
16 SGTC_2560 caryophyllene 3.79 100.0 µM 1.13E-4 20 7.57E-5
17 SGTC_40 0327-0103 3.68 19.8 µM 1.73E-4 40 1.18E-4
18 SGTC_1357 1503-1303 3.67 39.5 µM 1.79E-4 41 1.22E-4
19 SGTC_271 rotenone 3.66 740.0 µM 1.85E-4 36 1.26E-4
20 SGTC_2383 9036125 3.59 200.0 µM 2.37E-4 9 1.64E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.87E-11 YIL056W VHR1 hom Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
0.114 3.06E-11 YJL200C ACO2 hom Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
0.109 2.76E-10 YEL023C_p YEL023C_p hom Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
0.107 5.10E-10 YDL001W RMD1 hom Cytoplasmic protein required for sporulation
0.106 7.76E-10 YER076C_p YER076C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
0.097 1.81E-8 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.095 3.17E-8 YBR186W PCH2 hom Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation
0.092 9.40E-8 YIL044C AGE2 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
0.091 1.30E-7 YNL038W GPI15 het Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
0.090 1.63E-7 YOR025W HST3 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.090 1.71E-7 YGR283C YGR283C hom Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
0.085 8.50E-7 YJR045C SSC1 het Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
0.084 1.09E-6 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.083 1.64E-6 YJL103C GSM1 hom Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis
0.082 2.01E-6 YPL255W BBP1 het Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p