YPL115C / BEM3

Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL115C / BEM3

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:3.73|P-value:4.76E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:-3.60|P-value:7.10E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:12.40|P-value:1.68E-28 ID:SGTC_104|Compound:0074-0074|FD-Score:-4.63|P-value:2.06E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:11.60|P-value:3.87E-25 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.86|P-value:3.19E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.19|P-value:1.02E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-4.03|P-value:1.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:5.98|P-value:6.02E-8 ID:SGTC_429|Compound:1112-0049|FD-Score:4.37|P-value:5.45E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.89|P-value:2.84E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.98|P-value:5.04E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:5.39|P-value:8.94E-7 ID:SGTC_565|Compound:1418-0001|FD-Score:3.96|P-value:2.28E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.85|P-value:3.24E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:7.59|P-value:8.92E-12 ID:SGTC_916|Compound:4466-0038|FD-Score:4.15|P-value:1.17E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.54|P-value:2.91E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:5.04|P-value:4.04E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.81|P-value:3.66E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.05|P-value:1.66E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.03|P-value:1.78E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.84|P-value:9.14E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.57|P-value:7.80E-4 ID:SGTC_1636|Compound:st007707|FD-Score:6.89|P-value:5.23E-10 ID:SGTC_1637|Compound:st007924|FD-Score:3.62|P-value:6.80E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.81|P-value:3.63E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.72|P-value:4.87E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.08|P-value:1.48E-4 ID:SGTC_1884|Compound:5633444|FD-Score:4.51|P-value:3.22E-5 ID:SGTC_1948|Compound:st076329|FD-Score:3.84|P-value:3.30E-4 ID:SGTC_1980|Compound:st071811|FD-Score:4.32|P-value:6.54E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.94|P-value:2.45E-4 ID:SGTC_2074|Compound:5212007|FD-Score:5.83|P-value:1.23E-7 ID:SGTC_2075|Compound:5212009|FD-Score:5.19|P-value:2.15E-6 ID:SGTC_2315|Compound:7733312|FD-Score:3.56|P-value:8.10E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.59|P-value:7.35E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.88|P-value:9.44E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:12.90|P-value:2.35E-30 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.58|P-value:7.59E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.01|P-value:1.94E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.52|P-value:9.23E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.01|P-value:1.89E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.93|P-value:2.51E-4 ID:SGTC_2745|Compound:rifampicin|FD-Score:4.05|P-value:1.68E-4 ID:SGTC_2912|Compound:7964298|FD-Score:5.15|P-value:2.50E-6 ID:SGTC_2998|Compound:9066762|FD-Score:4.43|P-value:4.32E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-5.60|P-value:3.43E-7 ID:SGTC_3166|Compound:9102297|FD-Score:4.68|P-value:1.70E-5 ID:SGTC_3186|Compound:9110464|FD-Score:4.41|P-value:4.62E-5 ID:SGTC_3202|Compound:9113761|FD-Score:5.25|P-value:1.67E-6 ID:SGTC_3219|Compound:9131112|FD-Score:4.96|P-value:5.61E-6 ID:SGTC_3266|Compound:9136860|FD-Score:4.23|P-value:8.87E-5 ID:SGTC_3285|Compound:9112818|FD-Score:3.94|P-value:2.40E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.87|P-value:3.08E-4 ID:SGTC_3311|Compound:9131146|FD-Score:9.99|P-value:4.54E-19 ID:SGTC_3321|Compound:9137793|FD-Score:7.87|P-value:1.52E-12 ID:SGTC_3350|Compound:9150499|FD-Score:5.06|P-value:3.70E-6 ID:SGTC_11|Compound:0438-0307|FD-Score:3.73|P-value:4.76E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:-3.60|P-value:7.10E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:12.40|P-value:1.68E-28 ID:SGTC_104|Compound:0074-0074|FD-Score:-4.63|P-value:2.06E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:11.60|P-value:3.87E-25 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.86|P-value:3.19E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.19|P-value:1.02E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-4.03|P-value:1.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:5.98|P-value:6.02E-8 ID:SGTC_429|Compound:1112-0049|FD-Score:4.37|P-value:5.45E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.89|P-value:2.84E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.98|P-value:5.04E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:5.39|P-value:8.94E-7 ID:SGTC_565|Compound:1418-0001|FD-Score:3.96|P-value:2.28E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.85|P-value:3.24E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:7.59|P-value:8.92E-12 ID:SGTC_916|Compound:4466-0038|FD-Score:4.15|P-value:1.17E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.54|P-value:2.91E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:5.04|P-value:4.04E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.81|P-value:3.66E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.05|P-value:1.66E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.03|P-value:1.78E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.84|P-value:9.14E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.57|P-value:7.80E-4 ID:SGTC_1636|Compound:st007707|FD-Score:6.89|P-value:5.23E-10 ID:SGTC_1637|Compound:st007924|FD-Score:3.62|P-value:6.80E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.81|P-value:3.63E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.72|P-value:4.87E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.08|P-value:1.48E-4 ID:SGTC_1884|Compound:5633444|FD-Score:4.51|P-value:3.22E-5 ID:SGTC_1948|Compound:st076329|FD-Score:3.84|P-value:3.30E-4 ID:SGTC_1980|Compound:st071811|FD-Score:4.32|P-value:6.54E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.94|P-value:2.45E-4 ID:SGTC_2074|Compound:5212007|FD-Score:5.83|P-value:1.23E-7 ID:SGTC_2075|Compound:5212009|FD-Score:5.19|P-value:2.15E-6 ID:SGTC_2315|Compound:7733312|FD-Score:3.56|P-value:8.10E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.59|P-value:7.35E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.88|P-value:9.44E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:12.90|P-value:2.35E-30 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.58|P-value:7.59E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.01|P-value:1.94E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.52|P-value:9.23E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.01|P-value:1.89E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.93|P-value:2.51E-4 ID:SGTC_2745|Compound:rifampicin|FD-Score:4.05|P-value:1.68E-4 ID:SGTC_2912|Compound:7964298|FD-Score:5.15|P-value:2.50E-6 ID:SGTC_2998|Compound:9066762|FD-Score:4.43|P-value:4.32E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-5.60|P-value:3.43E-7 ID:SGTC_3166|Compound:9102297|FD-Score:4.68|P-value:1.70E-5 ID:SGTC_3186|Compound:9110464|FD-Score:4.41|P-value:4.62E-5 ID:SGTC_3202|Compound:9113761|FD-Score:5.25|P-value:1.67E-6 ID:SGTC_3219|Compound:9131112|FD-Score:4.96|P-value:5.61E-6 ID:SGTC_3266|Compound:9136860|FD-Score:4.23|P-value:8.87E-5 ID:SGTC_3285|Compound:9112818|FD-Score:3.94|P-value:2.40E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.87|P-value:3.08E-4 ID:SGTC_3311|Compound:9131146|FD-Score:9.99|P-value:4.54E-19 ID:SGTC_3321|Compound:9137793|FD-Score:7.87|P-value:1.52E-12 ID:SGTC_3350|Compound:9150499|FD-Score:5.06|P-value:3.70E-6

Top fitness defect scores for YPL115C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2584 androsterone 12.90 48.7 µM 2.35E-30 cell wall 11 3.91E-38
2 SGTC_58 0957-0393 12.40 9.0 µM 1.68E-28 cell wall 47 8.93E-36
3 SGTC_138 0850-0259 11.60 40.9 µM 3.87E-25 cell wall 30 1.69E-31
4 SGTC_3311 9131146 9.99 2.9 µM 4.54E-19 cell wall 16 8.80E-24
5 SGTC_3321 9137793 7.87 26.8 µM 1.52E-12 cell wall 10 1.71E-15
6 SGTC_863 0466-0191 7.59 129.0 µM 8.92E-12 cell wall 20 1.61E-14
7 SGTC_1636 st007707 6.89 68.9 µM 5.23E-10 16 2.81E-12
8 SGTC_304 1310-0298 5.98 97.0 µM 6.02E-8 cell wall 20 1.15E-9
9 SGTC_2582 agaric acid 5.88 100.0 µM 9.44E-8 redox potentiating 164 2.03E-9
10 SGTC_2074 5212007 5.83 75.4 µM 1.23E-7 3 2.85E-9
11 SGTC_537 1334-0398 5.39 209.0 µM 8.94E-7 5 3.49E-8
12 SGTC_3202 9113761 5.25 49.5 µM 1.67E-6 18 7.72E-8
13 SGTC_2075 5212009 5.19 200.0 µM 2.15E-6 2 1.06E-7
14 SGTC_2912 7964298 5.15 10.9 µM 2.50E-6 cell wall 64 1.28E-7
15 SGTC_3350 9150499 5.06 3.6 µM 3.70E-6 cell wall 26 2.10E-7
16 SGTC_951 1171-0579 5.04 82.7 µM 4.04E-6 cell wall 44 2.35E-7
17 SGTC_514 4466-0038 4.98 2.6 nM 5.04E-6 cell wall 108 3.10E-7
18 SGTC_3219 9131112 4.96 49.5 µM 5.61E-6 Golgi 22 3.55E-7
19 SGTC_1109 tnp00067 4.84 928.3 nM 9.14E-6 5 6.58E-7
20 SGTC_3166 9102297 4.68 49.5 µM 1.70E-5 Golgi 22 1.44E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.498 2.43E-209 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.461 1.39E-176 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.446 1.98E-163 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.426 2.96E-148 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.422 7.13E-145 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.419 1.31E-142 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.412 2.25E-137 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.411 4.67E-137 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.409 1.27E-135 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.401 5.62E-130 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.395 1.17E-125 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.377 1.11E-113 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.377 1.80E-113 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.362 3.29E-104 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.343 4.07E-93 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2