YPL119C / DBP1

Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL119C / DBP1

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:3.84|P-value:1.26E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.56|P-value:4.13E-5 ID:SGTC_164|Compound:4100-3994|FD-Score:6.44|P-value:8.02E-13 ID:SGTC_207|Compound:4185-0010|FD-Score:3.65|P-value:3.13E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.17|P-value:6.39E-12 ID:SGTC_376|Compound:3970-0862|FD-Score:3.23|P-value:2.04E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.66|P-value:3.02E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.10|P-value:3.06E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.69|P-value:2.28E-5 ID:SGTC_798|Compound:1287-0182|FD-Score:4.00|P-value:5.77E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:-2.90|P-value:6.81E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.27|P-value:1.71E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:2.90|P-value:7.59E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:2.94|P-value:6.48E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.60|P-value:1.92E-7 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.09|P-value:1.20E-8 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.79|P-value:1.39E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.90|P-value:9.58E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.09|P-value:3.62E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.25|P-value:1.84E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.14|P-value:2.94E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.03|P-value:4.62E-4 ID:SGTC_1793|Compound:st048256|FD-Score:3.30|P-value:1.54E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.05|P-value:4.26E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.04|P-value:4.45E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.30|P-value:1.52E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-2.83|P-value:8.90E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.90|P-value:9.85E-6 ID:SGTC_2094|Compound:5332512|FD-Score:2.97|P-value:5.71E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-4.26|P-value:1.26E-6 ID:SGTC_2099|Compound:5377090|FD-Score:-4.42|P-value:5.27E-7 ID:SGTC_2103|Compound:5378138|FD-Score:4.85|P-value:5.24E-8 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.31|P-value:1.45E-4 ID:SGTC_2197|Compound:6588183|FD-Score:-2.93|P-value:6.01E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.45|P-value:6.86E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.08|P-value:3.77E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.43|P-value:7.60E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.04|P-value:4.31E-4 ID:SGTC_2363|Compound:9053361|FD-Score:6.00|P-value:2.28E-11 ID:SGTC_2366|Compound:9070441|FD-Score:4.95|P-value:2.85E-8 ID:SGTC_2398|Compound:5228213|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:2.87|P-value:8.43E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.58|P-value:3.88E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.20|P-value:1.99E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.15|P-value:2.87E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.95|P-value:7.57E-6 ID:SGTC_2805|Compound:7950783|FD-Score:-3.25|P-value:1.62E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.44|P-value:8.28E-5 ID:SGTC_2861|Compound:9024595|FD-Score:2.83|P-value:9.83E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.85|P-value:8.98E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.84|P-value:9.53E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.20|P-value:2.11E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:3.84|P-value:1.26E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.56|P-value:4.13E-5 ID:SGTC_164|Compound:4100-3994|FD-Score:6.44|P-value:8.02E-13 ID:SGTC_207|Compound:4185-0010|FD-Score:3.65|P-value:3.13E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.17|P-value:6.39E-12 ID:SGTC_376|Compound:3970-0862|FD-Score:3.23|P-value:2.04E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.66|P-value:3.02E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.10|P-value:3.06E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.69|P-value:2.28E-5 ID:SGTC_798|Compound:1287-0182|FD-Score:4.00|P-value:5.77E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:-2.90|P-value:6.81E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.27|P-value:1.71E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:2.90|P-value:7.59E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:2.94|P-value:6.48E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.60|P-value:1.92E-7 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.09|P-value:1.20E-8 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.79|P-value:1.39E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.90|P-value:9.58E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.09|P-value:3.62E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.25|P-value:1.84E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.14|P-value:2.94E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.03|P-value:4.62E-4 ID:SGTC_1793|Compound:st048256|FD-Score:3.30|P-value:1.54E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.05|P-value:4.26E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.04|P-value:4.45E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.30|P-value:1.52E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-2.83|P-value:8.90E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.90|P-value:9.85E-6 ID:SGTC_2094|Compound:5332512|FD-Score:2.97|P-value:5.71E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-4.26|P-value:1.26E-6 ID:SGTC_2099|Compound:5377090|FD-Score:-4.42|P-value:5.27E-7 ID:SGTC_2103|Compound:5378138|FD-Score:4.85|P-value:5.24E-8 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.31|P-value:1.45E-4 ID:SGTC_2197|Compound:6588183|FD-Score:-2.93|P-value:6.01E-4 ID:SGTC_2220|Compound:6782180|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.45|P-value:6.86E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.08|P-value:3.77E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.43|P-value:7.60E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.04|P-value:4.31E-4 ID:SGTC_2363|Compound:9053361|FD-Score:6.00|P-value:2.28E-11 ID:SGTC_2366|Compound:9070441|FD-Score:4.95|P-value:2.85E-8 ID:SGTC_2398|Compound:5228213|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:2.87|P-value:8.43E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.58|P-value:3.88E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.20|P-value:1.99E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.15|P-value:2.87E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.95|P-value:7.57E-6 ID:SGTC_2805|Compound:7950783|FD-Score:-3.25|P-value:1.62E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.44|P-value:8.28E-5 ID:SGTC_2861|Compound:9024595|FD-Score:2.83|P-value:9.83E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.85|P-value:8.98E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.84|P-value:9.53E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.20|P-value:2.11E-6

Top fitness defect scores for YPL119C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_164 4100-3994 6.44 89.6 µM 8.02E-13 4 6.04E-11
2 SGTC_263 alizarin 6.17 107.9 µM 6.39E-12 iron homeostasis 24 3.39E-10
3 SGTC_2363 9053361 6.00 176.0 µM 2.28E-11 Golgi 6 9.79E-10
4 SGTC_1181 1222-0449 5.09 220.0 µM 1.20E-8 12 1.81E-7
5 SGTC_2366 9070441 4.95 60.6 µM 2.85E-8 12 3.72E-7
6 SGTC_2103 5378138 4.85 10.0 µM 5.24E-8 5 6.17E-7
7 SGTC_3328 9140624 4.20 29.0 µM 2.11E-6 13 1.35E-5
8 SGTC_798 1287-0182 4.00 476.0 µM 5.77E-6 22 3.13E-5
9 SGTC_2708 st078559 3.95 56.0 µM 7.57E-6 4 3.93E-5
10 SGTC_1241 0364-0022 3.90 50.8 µM 9.58E-6 ERG2 30 4.79E-5
11 SGTC_2076 5212012 3.90 200.0 µM 9.85E-6 6 4.90E-5
12 SGTC_115 0527-0192 3.84 112.0 µM 1.26E-5 78 6.04E-5
13 SGTC_668 1682-0499 3.66 62.6 µM 3.02E-5 27 1.25E-4
14 SGTC_207 4185-0010 3.65 4.5 µM 3.13E-5 endomembrane recycling 105 1.29E-4
15 SGTC_2814 7977482 3.44 43.0 µM 8.28E-5 72 2.92E-4
16 SGTC_2220 6782180 3.38 200.0 µM 1.08E-4 RNA processing & uracil transport 14 3.65E-4
17 SGTC_2130 NSC 7 3.31 2.5 µM 1.45E-4 copper-dependent oxidative stress 63 4.67E-4
18 SGTC_2063 5246145 3.30 200.0 µM 1.52E-4 46 4.87E-4
19 SGTC_1793 st048256 3.30 72.2 µM 1.54E-4 11 4.92E-4
20 SGTC_2398 5228213 3.28 48.8 µM 1.66E-4 iron homeostasis 76 5.23E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 2.29E-19 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.132 1.32E-14 YDR110W FOB1 hom Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases
0.128 8.21E-14 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.126 2.08E-13 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.121 1.65E-12 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.120 3.58E-12 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.117 1.26E-11 YLR449W FPR4 hom Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
0.113 4.96E-11 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.111 1.07E-10 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.107 4.22E-10 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.107 4.53E-10 YKL063C_p YKL063C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
0.103 2.12E-9 YLR132C USB1 het Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
0.102 3.04E-9 YNL270C ALP1 hom Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
0.101 5.21E-9 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.099 9.34E-9 YLR259C HSP60 het Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated