YPL120W / VPS30

Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL120W / VPS30

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.57|P-value:6.90E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.33|P-value:1.84E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.47|P-value:4.79E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-9.38|P-value:3.29E-19 ID:SGTC_59|Compound:0330-0160|FD-Score:3.60|P-value:3.01E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.39|P-value:6.20E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.11|P-value:4.61E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:3.53|P-value:3.85E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:2.47E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:15.60|P-value:3.97E-50 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.67|P-value:1.13E-10 ID:SGTC_227|Compound:fluconazole|FD-Score:4.76|P-value:2.90E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.33|P-value:1.84E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.47|P-value:1.22E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:3.72|P-value:1.98E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.38|P-value:6.28E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:5.39|P-value:1.45E-7 ID:SGTC_567|Compound:1080-0542|FD-Score:4.53|P-value:8.21E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:5.92|P-value:9.09E-9 ID:SGTC_582|Compound:0987-0078|FD-Score:-4.06|P-value:6.37E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:3.52|P-value:3.97E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:5.59|P-value:5.31E-8 ID:SGTC_725|Compound:1497-0963|FD-Score:3.64|P-value:2.61E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.28|P-value:8.70E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:4.46|P-value:1.08E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:6.58|P-value:1.92E-10 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.40|P-value:6.06E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.56|P-value:3.50E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:7.26|P-value:2.57E-12 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.88|P-value:1.27E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:-3.53|P-value:4.27E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.45|P-value:1.07E-7 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.52|P-value:3.97E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.62|P-value:2.83E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.77|P-value:1.67E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.94|P-value:1.28E-6 ID:SGTC_1658|Compound:st012944|FD-Score:4.93|P-value:1.38E-6 ID:SGTC_1687|Compound:st020778|FD-Score:12.00|P-value:2.00E-30 ID:SGTC_1704|Compound:st028805|FD-Score:5.01|P-value:9.14E-7 ID:SGTC_1712|Compound:st032288|FD-Score:3.37|P-value:6.62E-4 ID:SGTC_1754|Compound:st042065|FD-Score:8.39|P-value:7.60E-16 ID:SGTC_1760|Compound:st045453|FD-Score:6.09|P-value:3.44E-9 ID:SGTC_1800|Compound:st050079|FD-Score:4.00|P-value:7.04E-5 ID:SGTC_1803|Compound:st050068|FD-Score:6.73|P-value:7.70E-11 ID:SGTC_1954|Compound:st077032|FD-Score:4.49|P-value:9.61E-6 ID:SGTC_1975|Compound:st070967|FD-Score:3.31|P-value:8.14E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.58|P-value:5.45E-8 ID:SGTC_2077|Compound:5212015|FD-Score:4.18|P-value:3.46E-5 ID:SGTC_2161|Compound:5662504|FD-Score:3.34|P-value:7.39E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.44|P-value:5.22E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.79|P-value:2.52E-6 ID:SGTC_2257|Compound:7960413|FD-Score:4.70|P-value:3.81E-6 ID:SGTC_2284|Compound:7945075|FD-Score:-3.35|P-value:7.85E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.61|P-value:2.87E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.81|P-value:1.41E-4 ID:SGTC_2439|Compound:5492030|FD-Score:5.26|P-value:2.78E-7 ID:SGTC_2445|Compound:5742597|FD-Score:3.83|P-value:1.33E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.26|P-value:9.33E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.33|P-value:7.41E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.06|P-value:5.42E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.34|P-value:1.87E-7 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.14|P-value:4.03E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.03|P-value:1.16E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.74|P-value:1.86E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.36|P-value:6.91E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.94|P-value:8.83E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-4.14|P-value:4.61E-5 ID:SGTC_2827|Compound:7993847|FD-Score:5.61|P-value:4.72E-8 ID:SGTC_2837|Compound:9008476|FD-Score:3.29|P-value:8.53E-4 ID:SGTC_2896|Compound:9052656|FD-Score:6.55|P-value:2.38E-10 ID:SGTC_2929|Compound:9013348|FD-Score:-5.20|P-value:4.52E-7 ID:SGTC_2938|Compound:9059746|FD-Score:4.49|P-value:9.79E-6 ID:SGTC_2966|Compound:9084041|FD-Score:4.90|P-value:1.58E-6 ID:SGTC_2967|Compound:9089538|FD-Score:-4.22|P-value:3.39E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.71|P-value:2.07E-4 ID:SGTC_2986|Compound:9022445|FD-Score:3.54|P-value:3.72E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.57|P-value:6.90E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.33|P-value:1.84E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.47|P-value:4.79E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-9.38|P-value:3.29E-19 ID:SGTC_59|Compound:0330-0160|FD-Score:3.60|P-value:3.01E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.39|P-value:6.20E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.11|P-value:4.61E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:3.53|P-value:3.85E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:2.47E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:15.60|P-value:3.97E-50 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.67|P-value:1.13E-10 ID:SGTC_227|Compound:fluconazole|FD-Score:4.76|P-value:2.90E-6 ID:SGTC_263|Compound:alizarin|FD-Score:4.33|P-value:1.84E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.47|P-value:1.22E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:3.72|P-value:1.98E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.38|P-value:6.28E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:5.39|P-value:1.45E-7 ID:SGTC_567|Compound:1080-0542|FD-Score:4.53|P-value:8.21E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:5.92|P-value:9.09E-9 ID:SGTC_582|Compound:0987-0078|FD-Score:-4.06|P-value:6.37E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:3.52|P-value:3.97E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:5.59|P-value:5.31E-8 ID:SGTC_725|Compound:1497-0963|FD-Score:3.64|P-value:2.61E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.28|P-value:8.70E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:4.46|P-value:1.08E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:6.58|P-value:1.92E-10 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.40|P-value:6.06E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.56|P-value:3.50E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:7.26|P-value:2.57E-12 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.88|P-value:1.27E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:-3.53|P-value:4.27E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.45|P-value:1.07E-7 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.52|P-value:3.97E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.62|P-value:2.83E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.77|P-value:1.67E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.94|P-value:1.28E-6 ID:SGTC_1658|Compound:st012944|FD-Score:4.93|P-value:1.38E-6 ID:SGTC_1687|Compound:st020778|FD-Score:12.00|P-value:2.00E-30 ID:SGTC_1704|Compound:st028805|FD-Score:5.01|P-value:9.14E-7 ID:SGTC_1712|Compound:st032288|FD-Score:3.37|P-value:6.62E-4 ID:SGTC_1754|Compound:st042065|FD-Score:8.39|P-value:7.60E-16 ID:SGTC_1760|Compound:st045453|FD-Score:6.09|P-value:3.44E-9 ID:SGTC_1800|Compound:st050079|FD-Score:4.00|P-value:7.04E-5 ID:SGTC_1803|Compound:st050068|FD-Score:6.73|P-value:7.70E-11 ID:SGTC_1954|Compound:st077032|FD-Score:4.49|P-value:9.61E-6 ID:SGTC_1975|Compound:st070967|FD-Score:3.31|P-value:8.14E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.58|P-value:5.45E-8 ID:SGTC_2077|Compound:5212015|FD-Score:4.18|P-value:3.46E-5 ID:SGTC_2161|Compound:5662504|FD-Score:3.34|P-value:7.39E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.44|P-value:5.22E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.79|P-value:2.52E-6 ID:SGTC_2257|Compound:7960413|FD-Score:4.70|P-value:3.81E-6 ID:SGTC_2284|Compound:7945075|FD-Score:-3.35|P-value:7.85E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.61|P-value:2.87E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.81|P-value:1.41E-4 ID:SGTC_2439|Compound:5492030|FD-Score:5.26|P-value:2.78E-7 ID:SGTC_2445|Compound:5742597|FD-Score:3.83|P-value:1.33E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.26|P-value:9.33E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.33|P-value:7.41E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.06|P-value:5.42E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.34|P-value:1.87E-7 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.14|P-value:4.03E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.03|P-value:1.16E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.74|P-value:1.86E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.36|P-value:6.91E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.94|P-value:8.83E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-4.14|P-value:4.61E-5 ID:SGTC_2827|Compound:7993847|FD-Score:5.61|P-value:4.72E-8 ID:SGTC_2837|Compound:9008476|FD-Score:3.29|P-value:8.53E-4 ID:SGTC_2896|Compound:9052656|FD-Score:6.55|P-value:2.38E-10 ID:SGTC_2929|Compound:9013348|FD-Score:-5.20|P-value:4.52E-7 ID:SGTC_2938|Compound:9059746|FD-Score:4.49|P-value:9.79E-6 ID:SGTC_2966|Compound:9084041|FD-Score:4.90|P-value:1.58E-6 ID:SGTC_2967|Compound:9089538|FD-Score:-4.22|P-value:3.39E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.71|P-value:2.07E-4 ID:SGTC_2986|Compound:9022445|FD-Score:3.54|P-value:3.72E-4

Top fitness defect scores for YPL120W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_206 4546-0025 15.60 72.0 µM 3.97E-50 NEO1 5 2.11E-55
2 SGTC_1687 st020778 12.00 55.4 µM 2.00E-30 36 1.58E-33
3 SGTC_1754 st042065 8.39 16.1 µM 7.60E-16 NEO1-PIK1 51 2.36E-17
4 SGTC_1332 1480-0653 7.26 17.5 µM 2.57E-12 TSC3-RPN4 2 1.92E-13
5 SGTC_2688 kanamycin b 7.03 96.7 µM 1.16E-11 calcium & mitochondrial duress 107 1.02E-12
6 SGTC_1803 st050068 6.73 52.4 µM 7.70E-11 NEO1-PIK1 61 8.28E-12
7 SGTC_221 trifluoperazine 6.67 10.3 µM 1.13E-10 NEO1-PIK1 105 1.27E-11
8 SGTC_1230 0170-0365 6.58 3.8 µM 1.92E-10 endomembrane recycling 15 2.28E-11
9 SGTC_2896 9052656 6.55 71.4 µM 2.38E-10 endomembrane recycling 32 2.89E-11
10 SGTC_1760 st045453 6.09 10.4 µM 3.44E-9 NEO1-PIK1 80 5.54E-10
11 SGTC_568 1193-0097 5.92 7.5 µM 9.09E-9 ERAD & cell cycle 61 1.62E-9
12 SGTC_2827 7993847 5.61 71.4 µM 4.72E-8 5 1.00E-8
13 SGTC_649 1123-0311 5.59 367.0 µM 5.31E-8 endomembrane recycling 29 1.14E-8
14 SGTC_2021 4019055 5.58 173.0 µM 5.45E-8 endomembrane recycling 52 1.18E-8
15 SGTC_1369 1866-0067 5.45 208.0 µM 1.07E-7 RPP1 & pyrimidine depletion 14 2.48E-8
16 SGTC_564 3555-0129 5.39 330.0 µM 1.45E-7 1 3.47E-8
17 SGTC_2629 bisabolol 5.34 100.0 µM 1.87E-7 6 4.60E-8
18 SGTC_2439 5492030 5.26 7.2 µM 2.78E-7 5 7.14E-8
19 SGTC_1704 st028805 5.01 70.8 µM 9.14E-7 3 2.66E-7
20 SGTC_1635 st007035 4.94 42.4 µM 1.28E-6 170 3.86E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.392 2.59E-123 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.375 1.38E-112 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.369 4.77E-109 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.316 1.09E-78 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.314 8.86E-78 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.313 4.60E-77 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.304 2.04E-72 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.299 2.43E-70 YLR360W VPS38 hom Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
0.299 4.92E-70 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.295 2.80E-68 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.289 1.67E-65 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.285 1.03E-63 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.283 1.00E-62 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.281 5.33E-62 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.279 6.54E-61 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism