YPL135W / ISU1

Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL135W / ISU1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.46|P-value:3.50E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:3.86|P-value:7.67E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.13|P-value:2.56E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:5.82|P-value:5.73E-9 ID:SGTC_252|Compound:miconazole|FD-Score:4.72|P-value:1.87E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.40|P-value:4.32E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.57|P-value:2.17E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.18|P-value:2.10E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:4.21|P-value:1.89E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:7.11|P-value:1.58E-12 ID:SGTC_381|Compound:3461-2219|FD-Score:3.25|P-value:7.32E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-5.89|P-value:3.32E-9 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.43|P-value:3.58E-4 ID:SGTC_462|Compound:ag-879|FD-Score:-4.12|P-value:2.45E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:-3.44|P-value:3.55E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-4.34|P-value:9.65E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.40|P-value:4.02E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.83|P-value:8.71E-5 ID:SGTC_675|Compound:1309-0189|FD-Score:4.83|P-value:1.12E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:4.89|P-value:8.25E-7 ID:SGTC_678|Compound:1218-2179|FD-Score:5.90|P-value:3.60E-9 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.65|P-value:2.55E-6 ID:SGTC_685|Compound:1278-0033|FD-Score:-5.19|P-value:1.65E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:3.32|P-value:5.68E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.30|P-value:5.75E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.30|P-value:6.18E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.23|P-value:7.89E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.40|P-value:4.31E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.28|P-value:6.62E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.80|P-value:1.00E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:-3.29|P-value:5.98E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.81|P-value:8.79E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.24|P-value:7.65E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.14|P-value:9.85E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.84|P-value:8.40E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.93|P-value:6.02E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.26|P-value:7.17E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.22|P-value:1.61E-5 ID:SGTC_1750|Compound:st081859|FD-Score:-4.04|P-value:3.45E-5 ID:SGTC_1778|Compound:st046819|FD-Score:-3.27|P-value:6.29E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.71|P-value:1.40E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:6.29|P-value:3.44E-10 ID:SGTC_1791|Compound:st048313|FD-Score:-3.32|P-value:5.37E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.25|P-value:7.33E-4 ID:SGTC_2071|Compound:5230951|FD-Score:5.05|P-value:3.68E-7 ID:SGTC_2294|Compound:7971431|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.34|P-value:5.33E-4 ID:SGTC_2339|Compound:9000433|FD-Score:4.02|P-value:4.16E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.51|P-value:4.45E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.70|P-value:1.34E-4 ID:SGTC_2477|Compound:5967686|FD-Score:-4.66|P-value:2.20E-6 ID:SGTC_2481|Compound:5478948|FD-Score:6.52|P-value:8.03E-11 ID:SGTC_2489|Compound:cantharidin|FD-Score:-4.21|P-value:1.70E-5 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.92|P-value:6.16E-5 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.96|P-value:5.24E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.19|P-value:8.95E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.34|P-value:5.36E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:5.43|P-value:5.18E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.65|P-value:1.76E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.48|P-value:3.24E-4 ID:SGTC_2827|Compound:7993847|FD-Score:-3.89|P-value:6.28E-5 ID:SGTC_2885|Compound:9057732|FD-Score:3.16|P-value:9.85E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.58|P-value:2.29E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.43|P-value:3.96E-4 ID:SGTC_2989|Compound:9073649|FD-Score:4.12|P-value:2.70E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.28|P-value:1.16E-7 ID:SGTC_3041|Compound:9091929|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.78|P-value:1.07E-4 ID:SGTC_3073|Compound:9114972|FD-Score:4.29|P-value:1.31E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.03|P-value:3.96E-5 ID:SGTC_3254|Compound:9136651|FD-Score:3.99|P-value:4.65E-5 ID:SGTC_6|Compound:0844-0013|FD-Score:3.46|P-value:3.50E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:3.86|P-value:7.67E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.13|P-value:2.56E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:5.82|P-value:5.73E-9 ID:SGTC_252|Compound:miconazole|FD-Score:4.72|P-value:1.87E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.40|P-value:4.32E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.57|P-value:2.17E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.18|P-value:2.10E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:4.21|P-value:1.89E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:7.11|P-value:1.58E-12 ID:SGTC_381|Compound:3461-2219|FD-Score:3.25|P-value:7.32E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-5.89|P-value:3.32E-9 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.43|P-value:3.58E-4 ID:SGTC_462|Compound:ag-879|FD-Score:-4.12|P-value:2.45E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:-3.44|P-value:3.55E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-4.34|P-value:9.65E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.40|P-value:4.02E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.83|P-value:8.71E-5 ID:SGTC_675|Compound:1309-0189|FD-Score:4.83|P-value:1.12E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:4.89|P-value:8.25E-7 ID:SGTC_678|Compound:1218-2179|FD-Score:5.90|P-value:3.60E-9 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.65|P-value:2.55E-6 ID:SGTC_685|Compound:1278-0033|FD-Score:-5.19|P-value:1.65E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:3.32|P-value:5.68E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.30|P-value:5.75E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.30|P-value:6.18E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.23|P-value:7.89E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.40|P-value:4.31E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.28|P-value:6.62E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.80|P-value:1.00E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:-3.29|P-value:5.98E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.81|P-value:8.79E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.24|P-value:7.65E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.14|P-value:9.85E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.84|P-value:8.40E-5 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.93|P-value:6.02E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.26|P-value:7.17E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.22|P-value:1.61E-5 ID:SGTC_1750|Compound:st081859|FD-Score:-4.04|P-value:3.45E-5 ID:SGTC_1778|Compound:st046819|FD-Score:-3.27|P-value:6.29E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.71|P-value:1.40E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:6.29|P-value:3.44E-10 ID:SGTC_1791|Compound:st048313|FD-Score:-3.32|P-value:5.37E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.25|P-value:7.33E-4 ID:SGTC_2071|Compound:5230951|FD-Score:5.05|P-value:3.68E-7 ID:SGTC_2294|Compound:7971431|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.34|P-value:5.33E-4 ID:SGTC_2339|Compound:9000433|FD-Score:4.02|P-value:4.16E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.51|P-value:4.45E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.70|P-value:1.34E-4 ID:SGTC_2477|Compound:5967686|FD-Score:-4.66|P-value:2.20E-6 ID:SGTC_2481|Compound:5478948|FD-Score:6.52|P-value:8.03E-11 ID:SGTC_2489|Compound:cantharidin|FD-Score:-4.21|P-value:1.70E-5 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.92|P-value:6.16E-5 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.96|P-value:5.24E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.19|P-value:8.95E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.34|P-value:5.36E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:5.43|P-value:5.18E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.65|P-value:1.76E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.48|P-value:3.24E-4 ID:SGTC_2827|Compound:7993847|FD-Score:-3.89|P-value:6.28E-5 ID:SGTC_2885|Compound:9057732|FD-Score:3.16|P-value:9.85E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.58|P-value:2.29E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.43|P-value:3.96E-4 ID:SGTC_2989|Compound:9073649|FD-Score:4.12|P-value:2.70E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.28|P-value:1.16E-7 ID:SGTC_3041|Compound:9091929|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.78|P-value:1.07E-4 ID:SGTC_3073|Compound:9114972|FD-Score:4.29|P-value:1.31E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.03|P-value:3.96E-5 ID:SGTC_3254|Compound:9136651|FD-Score:3.99|P-value:4.65E-5

Top fitness defect scores for YPL135W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_380 3970-0968 7.11 6.0 µM 1.58E-12 1 5.91E-13
2 SGTC_2481 5478948 6.52 45.2 µM 8.03E-11 PDR1 6 3.48E-11
3 SGTC_1788 fluconazole 6.29 20.0 µM 3.44E-10 azole & statin 27 1.57E-10
4 SGTC_678 1218-2179 5.90 85.9 µM 3.60E-9 RNA pol III & RNase P/MRP 1 1.80E-9
5 SGTC_217 ketoconazole 5.82 989.0 nM 5.73E-9 ergosterol depletion effects on membrane 6 2.91E-9
6 SGTC_2756 carbadox 5.43 75.1 µM 5.18E-8 24 2.86E-8
7 SGTC_3037 9091026 5.28 49.5 µM 1.16E-7 NEO1-PIK1 61 6.60E-8
8 SGTC_2071 5230951 5.05 39.9 µM 3.68E-7 TSC3-RPN4 6 2.19E-7
9 SGTC_676 falcarinol 4.89 140.0 µM 8.25E-7 34 5.05E-7
10 SGTC_675 1309-0189 4.83 238.0 µM 1.12E-6 7 6.92E-7
11 SGTC_252 miconazole 4.72 1.3 nM 1.87E-6 ergosterol depletion effects on membrane 28 1.18E-6
12 SGTC_682 0kpi-0099 4.65 17.0 µM 2.55E-6 46 1.63E-6
13 SGTC_3073 9114972 4.29 49.5 µM 1.31E-5 60S ribosome export 6 8.87E-6
14 SGTC_359 0081-0058 4.21 15.9 µM 1.89E-5 60S ribosome export 34 1.30E-5
15 SGTC_346 4513-0042 4.18 7.2 µM 2.10E-5 azole & statin 35 1.45E-5
16 SGTC_178 4092-0806 4.13 87.5 µM 2.56E-5 ergosterol depletion effects on membrane 86 1.78E-5
17 SGTC_2989 9073649 4.12 71.4 µM 2.70E-5 30 1.88E-5
18 SGTC_3152 9099253 4.03 49.5 µM 3.96E-5 NEO1-PIK1 125 2.80E-5
19 SGTC_2339 9000433 4.02 200.0 µM 4.16E-5 36 2.95E-5
20 SGTC_3254 9136651 3.99 49.5 µM 4.65E-5 DNA damage response 23 3.30E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 3.93E-11 YLR374C_d YLR374C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
0.110 1.74E-10 YCL017C NFS1 het Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
0.109 2.39E-10 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.107 4.72E-10 YHR045W_p YHR045W_p hom Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
0.103 2.37E-9 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.098 1.25E-8 YDL118W_d YDL118W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.097 1.64E-8 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.097 1.72E-8 YBR294W SUL1 hom High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
0.095 4.08E-8 YLL036C PRP19 het Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
0.090 2.03E-7 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.088 3.85E-7 YHL027W RIM101 hom Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
0.086 5.74E-7 YDR524C AGE1 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
0.085 8.81E-7 YCL031C RRP7 het Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
0.084 9.58E-7 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.083 1.68E-6 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress