HIPHOP chemogenomics database

YPL135W / ISU1

Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL135W / ISU1

Click on Significant Values for Screen Details

Top fitness defect scores for YPL135W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_380	3970-0968	7.11	6.0 µM	1.58E-12		1	5.91E-13
2	SGTC_2481	5478948	6.52	45.2 µM	8.03E-11	PDR1	6	3.48E-11
3	SGTC_1788	fluconazole	6.29	20.0 µM	3.44E-10	azole & statin	27	1.57E-10
4	SGTC_678	1218-2179	5.90	85.9 µM	3.60E-9	RNA pol III & RNase P/MRP	1	1.80E-9
5	SGTC_217	ketoconazole	5.82	989.0 nM	5.73E-9	ergosterol depletion effects on membrane	6	2.91E-9
6	SGTC_2756	carbadox	5.43	75.1 µM	5.18E-8		24	2.86E-8
7	SGTC_3037	9091026	5.28	49.5 µM	1.16E-7	NEO1-PIK1	61	6.60E-8
8	SGTC_2071	5230951	5.05	39.9 µM	3.68E-7	TSC3-RPN4	6	2.19E-7
9	SGTC_676	falcarinol	4.89	140.0 µM	8.25E-7		34	5.05E-7
10	SGTC_675	1309-0189	4.83	238.0 µM	1.12E-6		7	6.92E-7
11	SGTC_252	miconazole	4.72	1.3 nM	1.87E-6	ergosterol depletion effects on membrane	28	1.18E-6
12	SGTC_682	0kpi-0099	4.65	17.0 µM	2.55E-6		46	1.63E-6
13	SGTC_3073	9114972	4.29	49.5 µM	1.31E-5	60S ribosome export	6	8.87E-6
14	SGTC_359	0081-0058	4.21	15.9 µM	1.89E-5	60S ribosome export	34	1.30E-5
15	SGTC_346	4513-0042	4.18	7.2 µM	2.10E-5	azole & statin	35	1.45E-5
16	SGTC_178	4092-0806	4.13	87.5 µM	2.56E-5	ergosterol depletion effects on membrane	86	1.78E-5
17	SGTC_2989	9073649	4.12	71.4 µM	2.70E-5		30	1.88E-5
18	SGTC_3152	9099253	4.03	49.5 µM	3.96E-5	NEO1-PIK1	125	2.80E-5
19	SGTC_2339	9000433	4.02	200.0 µM	4.16E-5		36	2.95E-5
20	SGTC_3254	9136651	3.99	49.5 µM	4.65E-5	DNA damage response	23	3.30E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.114	3.93E-11	YLR374C_d	YLR374C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
0.110	1.74E-10	YCL017C	NFS1	het	Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
0.109	2.39E-10	YML126C	ERG13	het	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.107	4.72E-10	YHR045W_p	YHR045W_p	hom	Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
0.103	2.37E-9	YNL294C	RIM21	hom	pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.098	1.25E-8	YDL118W_d	YDL118W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.097	1.64E-8	YMR154C	RIM13	hom	Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.097	1.72E-8	YBR294W	SUL1	hom	High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
0.095	4.08E-8	YLL036C	PRP19	het	Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
0.090	2.03E-7	YOR275C	RIM20	hom	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.088	3.85E-7	YHL027W	RIM101	hom	Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
0.086	5.74E-7	YDR524C	AGE1	hom	ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
0.085	8.81E-7	YCL031C	RRP7	het	Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
0.084	9.58E-7	YGR122W	YGR122W	hom	Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.083	1.68E-6	YML075C	HMG1	hom	HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress