Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YPL135W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.114 | 3.93E-11 | YLR374C_d | YLR374C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W |
0.110 | 1.74E-10 | YCL017C | NFS1 | het | Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria |
0.109 | 2.39E-10 | YML126C | ERG13 | het | 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis |
0.107 | 4.72E-10 | YHR045W_p | YHR045W_p | hom | Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum |
0.103 | 2.37E-9 | YNL294C | RIM21 | hom | pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH |
0.098 | 1.25E-8 | YDL118W_d | YDL118W_d | hom | Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein |
0.097 | 1.64E-8 | YMR154C | RIM13 | hom | Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB |
0.097 | 1.72E-8 | YBR294W | SUL1 | hom | High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates |
0.095 | 4.08E-8 | YLL036C | PRP19 | het | Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain |
0.090 | 2.03E-7 | YOR275C | RIM20 | hom | Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation |
0.088 | 3.85E-7 | YHL027W | RIM101 | hom | Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC |
0.086 | 5.74E-7 | YDR524C | AGE1 | hom | ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif |
0.085 | 8.81E-7 | YCL031C | RRP7 | het | Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress |
0.084 | 9.58E-7 | YGR122W | YGR122W | hom | Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress |
0.083 | 1.68E-6 | YML075C | HMG1 | hom | HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress |