YPL140C / MKK2

MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL140C / MKK2

Click on Significant Values for Screen Details ID:SGTC_93|Compound:0109-0045|FD-Score:4.17|P-value:1.37E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:-3.09|P-value:9.31E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-4.36|P-value:5.45E-6 ID:SGTC_433|Compound:pimozide|FD-Score:-3.27|P-value:4.82E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.88|P-value:4.67E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:4.31|P-value:7.09E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:4.58|P-value:1.96E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:3.85|P-value:5.27E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.40|P-value:3.08E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.16|P-value:7.27E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.72|P-value:8.83E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:-4.35|P-value:5.81E-6 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.93|P-value:3.51E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.36|P-value:5.68E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.52|P-value:1.99E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.43|P-value:2.77E-4 ID:SGTC_1683|Compound:st010134|FD-Score:5.64|P-value:6.58E-9 ID:SGTC_1684|Compound:st024538|FD-Score:3.18|P-value:6.93E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.35|P-value:3.67E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.37|P-value:3.51E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-5.68|P-value:5.29E-9 ID:SGTC_2069|Compound:5228469|FD-Score:4.50|P-value:2.88E-6 ID:SGTC_2104|Compound:5389443|FD-Score:3.42|P-value:2.83E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.96|P-value:3.41E-5 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.37|P-value:3.44E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.86|P-value:4.97E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.36|P-value:3.47E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.57|P-value:1.59E-4 ID:SGTC_2505|Compound:galangin|FD-Score:5.37|P-value:3.24E-8 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.08|P-value:1.99E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.58|P-value:1.57E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.70|P-value:9.64E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.43|P-value:2.79E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_2706|Compound:st078239|FD-Score:3.27|P-value:5.06E-4 ID:SGTC_2822|Compound:7994718|FD-Score:7.94|P-value:6.42E-16 ID:SGTC_2823|Compound:7987624|FD-Score:-5.49|P-value:1.58E-8 ID:SGTC_2869|Compound:9037858|FD-Score:5.81|P-value:2.46E-9 ID:SGTC_2893|Compound:9048488|FD-Score:3.28|P-value:4.86E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-4.30|P-value:7.13E-6 ID:SGTC_2947|Compound:9072639|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_2949|Compound:9072542|FD-Score:5.09|P-value:1.50E-7 ID:SGTC_2993|Compound:9054257|FD-Score:3.83|P-value:5.69E-5 ID:SGTC_3020|Compound:9089140|FD-Score:4.06|P-value:2.17E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:4.17|P-value:1.37E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:-3.09|P-value:9.31E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-4.36|P-value:5.45E-6 ID:SGTC_433|Compound:pimozide|FD-Score:-3.27|P-value:4.82E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.88|P-value:4.67E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:4.31|P-value:7.09E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:4.58|P-value:1.96E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:3.85|P-value:5.27E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.40|P-value:3.08E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-3.16|P-value:7.27E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.72|P-value:8.83E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:-4.35|P-value:5.81E-6 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.93|P-value:3.51E-7 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.36|P-value:5.68E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.52|P-value:1.99E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.43|P-value:2.77E-4 ID:SGTC_1683|Compound:st010134|FD-Score:5.64|P-value:6.58E-9 ID:SGTC_1684|Compound:st024538|FD-Score:3.18|P-value:6.93E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.35|P-value:3.67E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.37|P-value:3.51E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-5.68|P-value:5.29E-9 ID:SGTC_2069|Compound:5228469|FD-Score:4.50|P-value:2.88E-6 ID:SGTC_2104|Compound:5389443|FD-Score:3.42|P-value:2.83E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.96|P-value:3.41E-5 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.37|P-value:3.44E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.86|P-value:4.97E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.36|P-value:3.47E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.57|P-value:1.59E-4 ID:SGTC_2505|Compound:galangin|FD-Score:5.37|P-value:3.24E-8 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.08|P-value:1.99E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.58|P-value:1.57E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.70|P-value:9.64E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.43|P-value:2.79E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_2706|Compound:st078239|FD-Score:3.27|P-value:5.06E-4 ID:SGTC_2822|Compound:7994718|FD-Score:7.94|P-value:6.42E-16 ID:SGTC_2823|Compound:7987624|FD-Score:-5.49|P-value:1.58E-8 ID:SGTC_2869|Compound:9037858|FD-Score:5.81|P-value:2.46E-9 ID:SGTC_2893|Compound:9048488|FD-Score:3.28|P-value:4.86E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-4.30|P-value:7.13E-6 ID:SGTC_2947|Compound:9072639|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_2949|Compound:9072542|FD-Score:5.09|P-value:1.50E-7 ID:SGTC_2993|Compound:9054257|FD-Score:3.83|P-value:5.69E-5 ID:SGTC_3020|Compound:9089140|FD-Score:4.06|P-value:2.17E-5

Top fitness defect scores for YPL140C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2822 7994718 7.94 71.4 µM 6.42E-16 2 1.02E-15
2 SGTC_2869 9037858 5.81 32.5 µM 2.46E-9 2 3.14E-9
3 SGTC_1683 st010134 5.64 710.0 nM 6.58E-9 6 8.29E-9
4 SGTC_2505 galangin 5.37 7.9 µM 3.24E-8 RPP1 & pyrimidine depletion 4 3.99E-8
5 SGTC_2949 9072542 5.09 2.2 µM 1.50E-7 TSC3-RPN4 11 1.81E-7
6 SGTC_1354 1498-1681 4.93 81.0 µM 3.51E-7 7 4.19E-7
7 SGTC_883 0850-0245 4.58 24.6 µM 1.96E-6 cell wall 19 2.29E-6
8 SGTC_2069 5228469 4.50 200.0 µM 2.88E-6 6 3.34E-6
9 SGTC_1355 1499-0035 4.36 1.7 µM 5.68E-6 10 6.52E-6
10 SGTC_841 0251-0215 4.31 91.2 µM 7.09E-6 65 8.11E-6
11 SGTC_93 0109-0045 4.17 59.2 µM 1.37E-5 DNA damage response 36 1.55E-5
12 SGTC_2540 epiandrosterone 4.08 100.0 µM 1.99E-5 55 2.25E-5
13 SGTC_3020 9089140 4.06 49.5 µM 2.17E-5 26 2.45E-5
14 SGTC_2413 inermin 3.96 26.1 µM 3.41E-5 53 3.82E-5
15 SGTC_663 1486-0733 3.88 13.9 µM 4.67E-5 cell wall signaling 53 5.21E-5
16 SGTC_906 1165-0455 3.85 526.0 µM 5.27E-5 13 5.86E-5
17 SGTC_2993 9054257 3.83 71.4 µM 5.69E-5 fatty acid desaturase (OLE1) 48 6.33E-5
18 SGTC_1273 0849-0096 3.72 14.1 µM 8.83E-5 RPP1 & pyrimidine depletion 29 9.77E-5
19 SGTC_2645 abamectin 3.70 100.0 µM 9.64E-5 heme requiring 39 1.07E-4
20 SGTC_2562 3',7'-dimethoxyflavone 3.58 64.9 µM 1.57E-4 48 1.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.17E-12 YEL056W HAT2 hom Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
0.107 4.45E-10 YGR121C MEP1 hom Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
0.103 1.92E-9 YJL028W YJL028W hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments
0.102 3.61E-9 YEL055C POL5 het DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
0.098 1.10E-8 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.097 1.79E-8 YLR101C_d YLR101C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
0.081 2.51E-6 YNL087W TCB2 hom ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud
0.081 2.69E-6 YIR018C-A_p YIR018C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.080 3.57E-6 YKR002W PAP1 het Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
0.078 6.33E-6 YOL111C MDY2 hom Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
0.074 1.63E-5 YHL042W_p YHL042W_p hom Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.074 1.85E-5 YNL068C FKH2 hom Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
0.071 3.70E-5 YGL113W SLD3 het Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.070 4.61E-5 YOL130W ALR1 het Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
0.070 5.27E-5 YMR258C ROY1 hom GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p