HIPHOP chemogenomics database
YPL142C_d 
Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YPL142C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.237 | 4.04E-44 | YPL143W | RPL33A | het | Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication |
| 0.185 | 2.88E-27 | YOL120C | RPL18A | het | Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication |
| 0.177 | 9.06E-25 | YLR076C_d | YLR076C_d | het | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 |
| 0.165 | 9.66E-22 | YPL131W | RPL5 | het | Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly |
| 0.155 | 1.66E-19 | YLR009W | RLP24 | het | Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp |
| 0.155 | 1.83E-19 | YGL030W | RPL30 | het | Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog |
| 0.148 | 7.97E-18 | YKL180W | RPL17A | het | Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| 0.136 | 3.40E-15 | YBR140C | IRA1 | het | GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication |
| 0.133 | 1.33E-14 | YLR075W | RPL10 | het | Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects |
| 0.124 | 7.75E-13 | YOR224C | RPB8 | het | RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III |
| 0.123 | 9.94E-13 | YJR007W | SUI2 | het | Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress |
| 0.117 | 9.21E-12 | YBR190W_d | YBR190W_d | het | Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W |
| 0.117 | 1.23E-11 | YJR123W | RPS5 | het | Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 |
| 0.117 | 1.25E-11 | YGL055W | OLE1 | het | Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria |
| 0.115 | 2.39E-11 | YDR429C | TIF35 | het | eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation |