YPL146C / NOP53

Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL146C / NOP53

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:3.00|P-value:6.62E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-3.93|P-value:1.13E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:3.82|P-value:2.18E-5 ID:SGTC_399|Compound:oxolinic acid|FD-Score:4.11|P-value:5.32E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.59|P-value:6.11E-5 ID:SGTC_472|Compound:calcifediol|FD-Score:3.09|P-value:4.71E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:2.99|P-value:6.98E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.23|P-value:2.43E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:-3.20|P-value:2.74E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.02|P-value:5.51E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.21|P-value:2.94E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.36|P-value:1.14E-15 ID:SGTC_782|Compound:0083-0095|FD-Score:3.17|P-value:3.53E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:4.27|P-value:2.38E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:3.01|P-value:6.45E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-3.03|P-value:5.40E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.88|P-value:1.41E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.66|P-value:3.10E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:6.39|P-value:3.74E-12 ID:SGTC_989|Compound:1501-0895|FD-Score:3.06|P-value:5.39E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:-4.12|P-value:4.35E-6 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.56|P-value:6.99E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.16|P-value:3.63E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.62|P-value:4.68E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.32|P-value:1.91E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.07|P-value:5.11E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.21|P-value:2.68E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.97|P-value:1.08E-5 ID:SGTC_1609|Compound:st001574|FD-Score:-3.16|P-value:3.27E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.23|P-value:2.82E-4 ID:SGTC_1644|Compound:st010134|FD-Score:4.41|P-value:1.17E-6 ID:SGTC_1662|Compound:st014054|FD-Score:-3.19|P-value:2.90E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-3.93|P-value:1.12E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.91|P-value:8.37E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.05|P-value:7.48E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.12|P-value:4.19E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.09|P-value:4.73E-4 ID:SGTC_2192|Compound:5915882|FD-Score:2.90|P-value:9.77E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.11|P-value:3.99E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.10|P-value:4.60E-4 ID:SGTC_2372|Compound:9071633|FD-Score:3.67|P-value:4.35E-5 ID:SGTC_2377|Compound:9073351|FD-Score:-3.29|P-value:1.90E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-3.58|P-value:5.64E-5 ID:SGTC_2379|Compound:9035213|FD-Score:-3.20|P-value:2.72E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.15|P-value:3.34E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-2.96|P-value:6.85E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.03|P-value:5.92E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.81|P-value:1.97E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.12|P-value:3.72E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.81|P-value:2.33E-5 ID:SGTC_2805|Compound:7950783|FD-Score:3.67|P-value:4.40E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.25|P-value:2.76E-6 ID:SGTC_2836|Compound:9010338|FD-Score:-4.36|P-value:1.29E-6 ID:SGTC_2843|Compound:9000894|FD-Score:3.83|P-value:2.04E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.15|P-value:3.76E-4 ID:SGTC_3257|Compound:9139206|FD-Score:3.63|P-value:5.12E-5 ID:SGTC_3346|Compound:9158254|FD-Score:4.10|P-value:5.71E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:3.00|P-value:6.62E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-3.93|P-value:1.13E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:3.82|P-value:2.18E-5 ID:SGTC_399|Compound:oxolinic acid|FD-Score:4.11|P-value:5.32E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.59|P-value:6.11E-5 ID:SGTC_472|Compound:calcifediol|FD-Score:3.09|P-value:4.71E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:2.99|P-value:6.98E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.23|P-value:2.43E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:-3.20|P-value:2.74E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.02|P-value:5.51E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.21|P-value:2.94E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.36|P-value:1.14E-15 ID:SGTC_782|Compound:0083-0095|FD-Score:3.17|P-value:3.53E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:4.27|P-value:2.38E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:3.01|P-value:6.45E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-3.03|P-value:5.40E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.88|P-value:1.41E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.66|P-value:3.10E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:6.39|P-value:3.74E-12 ID:SGTC_989|Compound:1501-0895|FD-Score:3.06|P-value:5.39E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:-4.12|P-value:4.35E-6 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.56|P-value:6.99E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.16|P-value:3.63E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.62|P-value:4.68E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.32|P-value:1.91E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.07|P-value:5.11E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.21|P-value:2.68E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.97|P-value:1.08E-5 ID:SGTC_1609|Compound:st001574|FD-Score:-3.16|P-value:3.27E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.23|P-value:2.82E-4 ID:SGTC_1644|Compound:st010134|FD-Score:4.41|P-value:1.17E-6 ID:SGTC_1662|Compound:st014054|FD-Score:-3.19|P-value:2.90E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-3.93|P-value:1.12E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.91|P-value:8.37E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.05|P-value:7.48E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.12|P-value:4.19E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.09|P-value:4.73E-4 ID:SGTC_2192|Compound:5915882|FD-Score:2.90|P-value:9.77E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.11|P-value:3.99E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.10|P-value:4.60E-4 ID:SGTC_2372|Compound:9071633|FD-Score:3.67|P-value:4.35E-5 ID:SGTC_2377|Compound:9073351|FD-Score:-3.29|P-value:1.90E-4 ID:SGTC_2378|Compound:9074213|FD-Score:-3.58|P-value:5.64E-5 ID:SGTC_2379|Compound:9035213|FD-Score:-3.20|P-value:2.72E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.15|P-value:3.34E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-2.96|P-value:6.85E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.03|P-value:5.92E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.81|P-value:1.97E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.12|P-value:3.72E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.81|P-value:2.33E-5 ID:SGTC_2805|Compound:7950783|FD-Score:3.67|P-value:4.40E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.25|P-value:2.76E-6 ID:SGTC_2836|Compound:9010338|FD-Score:-4.36|P-value:1.29E-6 ID:SGTC_2843|Compound:9000894|FD-Score:3.83|P-value:2.04E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.15|P-value:3.76E-4 ID:SGTC_3257|Compound:9139206|FD-Score:3.63|P-value:5.12E-5 ID:SGTC_3346|Compound:9158254|FD-Score:4.10|P-value:5.71E-6

Top fitness defect scores for YPL146C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_903 1134-0042 6.39 114.0 µM 3.74E-12 1 8.30E-11 1.01
2 SGTC_879 0831-1030 4.66 162.0 µM 3.10E-7 13 1.61E-6 0.04
3 SGTC_1644 st010134 4.41 20.9 µM 1.17E-6 mitochondrial processes 3 5.16E-6 0.21
4 SGTC_802 4112-3352 4.27 742.0 µM 2.38E-6 2 9.59E-6 0.42
5 SGTC_2806 7952793 4.25 58.4 µM 2.76E-6 3 1.09E-5 0.05
6 SGTC_399 oxolinic acid 4.11 75.0 µM 5.32E-6 2 1.94E-5 0.41
7 SGTC_3346 9158254 4.10 43.6 µM 5.71E-6 9 2.06E-5 0.04
8 SGTC_2126 5251174 4.05 33.9 µM 7.48E-6 8 2.61E-5 0.38
9 SGTC_1232 0279-0259 3.97 161.0 µM 1.08E-5 4 3.61E-5 0.03
10 SGTC_2843 9000894 3.83 45.5 µM 2.04E-5 2 6.28E-5 0.32
11 SGTC_374 0880-0231 3.82 82.3 µM 2.18E-5 iron homeostasis 9 6.67E-5 0.15
12 SGTC_2629 bisabolol 3.81 100.0 µM 2.33E-5 10 7.06E-5 0.05
13 SGTC_2372 9071633 3.67 200.0 µM 4.35E-5 2 1.22E-4 0.16
14 SGTC_2805 7950783 3.67 58.4 µM 4.40E-5 10 1.23E-4 0.06
15 SGTC_3257 9139206 3.63 33.0 µM 5.12E-5 8 1.41E-4 0.10
16 SGTC_404 fumagillin 3.59 50.0 µM 6.11E-5 7 1.64E-4 0.04
17 SGTC_1009 4106-0057 3.56 9.9 µM 6.99E-5 12 1.85E-4 0.08
18 SGTC_1200 1643-0193 3.32 18.4 µM 1.91E-4 10 4.46E-4 0.07
19 SGTC_1642 st009582 3.23 77.7 µM 2.82E-4 RPP1 & pyrimidine depletion 12 6.29E-4 0.01
20 SGTC_626 0214-0009 3.21 33.0 µM 2.94E-4 copper-dependent oxidative stress 9 6.53E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 3.99E-13 YOR148C SPP2 het Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
0.121 2.59E-12 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.110 1.43E-10 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
0.109 2.32E-10 YBL019W APN2 hom Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
0.105 1.22E-9 YNL132W KRE33 het Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.101 4.28E-9 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.095 3.59E-8 YEL015W EDC3 hom Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress
0.092 8.89E-8 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.092 9.21E-8 YOR206W NOC2 het Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
0.090 1.95E-7 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.084 1.17E-6 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.083 1.38E-6 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.082 2.05E-6 YER127W LCP5 het Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
0.081 2.35E-6 YGL097W SRM1 het Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.081 2.69E-6 YGL018C JAC1 het Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix