YPL153C / RAD53

Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL153C / RAD53

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:7.60|P-value:6.33E-15 ID:SGTC_58|Compound:0957-0393|FD-Score:7.21|P-value:1.34E-13 ID:SGTC_64|Compound:000l-0018|FD-Score:6.77|P-value:3.16E-12 ID:SGTC_67|Compound:0335-0847|FD-Score:12.10|P-value:5.93E-35 ID:SGTC_103|Compound:0844-0104|FD-Score:5.17|P-value:7.45E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:7.17|P-value:1.80E-13 ID:SGTC_138|Compound:0850-0259|FD-Score:5.44|P-value:1.64E-8 ID:SGTC_158|Compound:k038-0087|FD-Score:3.68|P-value:8.77E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.61|P-value:1.16E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.20|P-value:5.54E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.54|P-value:1.52E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.04|P-value:1.99E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:3.33|P-value:3.47E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.32|P-value:3.51E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.39|P-value:2.71E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.06|P-value:8.78E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.89|P-value:3.77E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:3.23|P-value:4.83E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.48|P-value:1.95E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.19|P-value:5.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.58|P-value:1.56E-6 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.16|P-value:6.40E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.82|P-value:4.90E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:-3.17|P-value:7.66E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-3.64|P-value:1.35E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.14|P-value:6.80E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-4.31|P-value:8.02E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.24|P-value:7.86E-6 ID:SGTC_1821|Compound:st053465|FD-Score:4.02|P-value:2.12E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-3.15|P-value:8.34E-4 ID:SGTC_1998|Compound:st075797|FD-Score:-5.18|P-value:1.01E-7 ID:SGTC_2009|Compound:4012297|FD-Score:3.91|P-value:3.47E-5 ID:SGTC_2012|Compound:4023501|FD-Score:4.21|P-value:9.07E-6 ID:SGTC_2017|Compound:5132927|FD-Score:-3.67|P-value:1.23E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.03|P-value:9.75E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.72|P-value:7.48E-5 ID:SGTC_2248|Compound:tofa|FD-Score:8.17|P-value:6.11E-17 ID:SGTC_2279|Compound:7948423|FD-Score:3.16|P-value:6.36E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-4.96|P-value:3.37E-7 ID:SGTC_2325|Compound:6158266|FD-Score:-3.92|P-value:4.30E-5 ID:SGTC_2348|Compound:9022543|FD-Score:3.36|P-value:3.01E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.25|P-value:5.80E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.27|P-value:4.23E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:-3.13|P-value:8.93E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.20|P-value:9.48E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.49|P-value:2.44E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:-3.25|P-value:5.89E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.05|P-value:9.39E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.12|P-value:9.29E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.81|P-value:4.97E-7 ID:SGTC_2844|Compound:9010103|FD-Score:7.20|P-value:1.40E-13 ID:SGTC_2850|Compound:9015429|FD-Score:-3.31|P-value:4.66E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.64|P-value:4.94E-9 ID:SGTC_2858|Compound:9025781|FD-Score:13.70|P-value:3.60E-44 ID:SGTC_2871|Compound:9038510|FD-Score:3.75|P-value:6.63E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.23|P-value:4.97E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-3.58|P-value:1.73E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.62|P-value:1.11E-4 ID:SGTC_2943|Compound:9068529|FD-Score:5.08|P-value:1.20E-7 ID:SGTC_2952|Compound:9073731|FD-Score:5.69|P-value:3.69E-9 ID:SGTC_3116|Compound:9124943|FD-Score:-3.45|P-value:2.78E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.19|P-value:5.58E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.89|P-value:3.70E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-3.95|P-value:3.95E-5 ID:SGTC_3269|Compound:9137823|FD-Score:-3.40|P-value:3.45E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-4.03|P-value:2.79E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:7.60|P-value:6.33E-15 ID:SGTC_58|Compound:0957-0393|FD-Score:7.21|P-value:1.34E-13 ID:SGTC_64|Compound:000l-0018|FD-Score:6.77|P-value:3.16E-12 ID:SGTC_67|Compound:0335-0847|FD-Score:12.10|P-value:5.93E-35 ID:SGTC_103|Compound:0844-0104|FD-Score:5.17|P-value:7.45E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:7.17|P-value:1.80E-13 ID:SGTC_138|Compound:0850-0259|FD-Score:5.44|P-value:1.64E-8 ID:SGTC_158|Compound:k038-0087|FD-Score:3.68|P-value:8.77E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.61|P-value:1.16E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.20|P-value:5.54E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.54|P-value:1.52E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.04|P-value:1.99E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:3.33|P-value:3.47E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.32|P-value:3.51E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.39|P-value:2.71E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.06|P-value:8.78E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.89|P-value:3.77E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:3.23|P-value:4.83E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.48|P-value:1.95E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.19|P-value:5.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.58|P-value:1.56E-6 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.16|P-value:6.40E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.82|P-value:4.90E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:-3.17|P-value:7.66E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-3.64|P-value:1.35E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.14|P-value:6.80E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-4.31|P-value:8.02E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.24|P-value:7.86E-6 ID:SGTC_1821|Compound:st053465|FD-Score:4.02|P-value:2.12E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-3.15|P-value:8.34E-4 ID:SGTC_1998|Compound:st075797|FD-Score:-5.18|P-value:1.01E-7 ID:SGTC_2009|Compound:4012297|FD-Score:3.91|P-value:3.47E-5 ID:SGTC_2012|Compound:4023501|FD-Score:4.21|P-value:9.07E-6 ID:SGTC_2017|Compound:5132927|FD-Score:-3.67|P-value:1.23E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.03|P-value:9.75E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.72|P-value:7.48E-5 ID:SGTC_2248|Compound:tofa|FD-Score:8.17|P-value:6.11E-17 ID:SGTC_2279|Compound:7948423|FD-Score:3.16|P-value:6.36E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-4.96|P-value:3.37E-7 ID:SGTC_2325|Compound:6158266|FD-Score:-3.92|P-value:4.30E-5 ID:SGTC_2348|Compound:9022543|FD-Score:3.36|P-value:3.01E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.25|P-value:5.80E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.27|P-value:4.23E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:-3.13|P-value:8.93E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.20|P-value:9.48E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.49|P-value:2.44E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:-3.25|P-value:5.89E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.05|P-value:9.39E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.12|P-value:9.29E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.81|P-value:4.97E-7 ID:SGTC_2844|Compound:9010103|FD-Score:7.20|P-value:1.40E-13 ID:SGTC_2850|Compound:9015429|FD-Score:-3.31|P-value:4.66E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.64|P-value:4.94E-9 ID:SGTC_2858|Compound:9025781|FD-Score:13.70|P-value:3.60E-44 ID:SGTC_2871|Compound:9038510|FD-Score:3.75|P-value:6.63E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.23|P-value:4.97E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-3.58|P-value:1.73E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.62|P-value:1.11E-4 ID:SGTC_2943|Compound:9068529|FD-Score:5.08|P-value:1.20E-7 ID:SGTC_2952|Compound:9073731|FD-Score:5.69|P-value:3.69E-9 ID:SGTC_3116|Compound:9124943|FD-Score:-3.45|P-value:2.78E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.19|P-value:5.58E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.89|P-value:3.70E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-3.95|P-value:3.95E-5 ID:SGTC_3269|Compound:9137823|FD-Score:-3.40|P-value:3.45E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-4.03|P-value:2.79E-5

Top fitness defect scores for YPL153C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2858 9025781 13.70 9.0 µM 3.60E-44 sphingolipid biosynthesis & PDR1 1 3.52E-43 5.99
2 SGTC_67 0335-0847 12.10 266.0 µM 5.93E-35 ubiquinone biosynthesis & proteasome 2 3.70E-34 2.76
3 SGTC_2248 tofa 8.17 1.1 µM 6.11E-17 sphingolipid biosynthesis & PDR1 8 1.55E-16 0.55
4 SGTC_36 0335-0881 7.60 14.2 µM 6.33E-15 ubiquinone biosynthesis & proteasome 9 1.45E-14 0.01
5 SGTC_58 0957-0393 7.21 9.0 µM 1.34E-13 cell wall 23 2.87E-13 0.26
6 SGTC_2844 9010103 7.20 26.0 µM 1.40E-13 3 3.00E-13 1.43
7 SGTC_137 0438-0332 7.17 85.4 µM 1.80E-13 ubiquinone biosynthesis & proteasome 15 3.82E-13 0.20
8 SGTC_64 000l-0018 6.77 272.1 µM 3.16E-12 ubiquinone biosynthesis & proteasome 25 6.28E-12 6.52E-4
9 SGTC_2952 9073731 5.69 13.8 µM 3.69E-9 tubulin folding & SWR complex 5 6.22E-9 0.40
10 SGTC_2851 9015922 5.64 71.4 µM 4.94E-9 2 8.28E-9 0.04
11 SGTC_138 0850-0259 5.44 40.9 µM 1.64E-8 cell wall 37 2.66E-8 0.00
12 SGTC_103 0844-0104 5.17 51.8 µM 7.45E-8 ERAD & cell cycle 33 1.17E-7 0.03
13 SGTC_2943 9068529 5.08 45.3 µM 1.20E-7 7 1.86E-7 0.10
14 SGTC_2778 tioconazole 4.81 65.1 nM 4.97E-7 ergosterol depletion effects on membrane 20 7.45E-7 0.07
15 SGTC_1161 k007-0610 4.58 110.0 µM 1.56E-6 ERAD & cell cycle 30 2.28E-6 0.11
16 SGTC_1800 st050079 4.24 29.1 µM 7.86E-6 NEO1-PIK1 22 1.10E-5 0.02
17 SGTC_2012 4023501 4.21 200.0 µM 9.07E-6 9 1.27E-5 0.00
18 SGTC_2651 1327-0036 4.20 5.0 µM 9.48E-6 45 1.32E-5 0.20
19 SGTC_514 4466-0038 4.04 2.6 nM 1.99E-5 cell wall 39 2.73E-5 0.06
20 SGTC_1821 st053465 4.02 65.6 µM 2.12E-5 2 2.89E-5 0.32

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.327 1.55E-84 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.311 2.92E-76 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.301 2.26E-71 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.298 1.36E-69 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.290 6.64E-66 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.284 1.70E-63 YJL155C FBP26 hom Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.282 1.92E-62 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.280 1.45E-61 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.271 2.15E-57 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.266 1.78E-55 YFR007W YFH7 hom Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases
0.261 4.03E-53 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.260 4.79E-53 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.256 3.64E-51 YPL126W NAN1 het U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.250 4.42E-49 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.246 3.31E-47 YDL020C RPN4 hom Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress