YPL157W / TGS1

Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL157W / TGS1

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:3.62|P-value:2.81E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.54|P-value:3.62E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.54|P-value:8.25E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.34|P-value:7.17E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.57|P-value:7.33E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:7.37|P-value:2.09E-12 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.57|P-value:6.47E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.77|P-value:3.16E-6 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.28|P-value:3.04E-7 ID:SGTC_264|Compound:promethazine|FD-Score:11.90|P-value:5.25E-29 ID:SGTC_289|Compound:k017-0060|FD-Score:3.29|P-value:8.25E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.35|P-value:6.84E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.53|P-value:3.77E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.46|P-value:4.83E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:5.18|P-value:4.72E-7 ID:SGTC_438|Compound:sphingosine|FD-Score:4.46|P-value:1.17E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.94|P-value:3.30E-11 ID:SGTC_512|Compound:prima-1|FD-Score:4.01|P-value:6.82E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.95|P-value:1.74E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.93|P-value:9.26E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:3.50|P-value:4.17E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.35|P-value:6.79E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:3.55|P-value:3.58E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.41|P-value:5.54E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.58|P-value:3.24E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.52|P-value:3.91E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.28|P-value:8.68E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.83|P-value:1.32E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.43|P-value:5.22E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.08|P-value:5.27E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.80|P-value:1.47E-4 ID:SGTC_1746|Compound:st038117|FD-Score:5.33|P-value:2.30E-7 ID:SGTC_1807|Compound:st050092|FD-Score:3.34|P-value:7.18E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.25|P-value:9.49E-4 ID:SGTC_1905|Compound:st060207|FD-Score:-3.46|P-value:9.16E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.31|P-value:2.56E-7 ID:SGTC_1947|Compound:st076332|FD-Score:4.27|P-value:2.46E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.23|P-value:2.90E-5 ID:SGTC_2289|Compound:7946404|FD-Score:6.01|P-value:7.07E-9 ID:SGTC_2402|Compound:5553605|FD-Score:6.16|P-value:3.22E-9 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.40|P-value:5.75E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:4.66|P-value:5.04E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.41|P-value:5.59E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.92|P-value:9.56E-5 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.31|P-value:7.85E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.96|P-value:1.31E-6 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.24|P-value:9.83E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:4.52|P-value:8.99E-6 ID:SGTC_2740|Compound:felodipine|FD-Score:3.55|P-value:3.58E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.48|P-value:1.08E-5 ID:SGTC_2982|Compound:9019404|FD-Score:3.44|P-value:5.16E-4 ID:SGTC_2994|Compound:9053520|FD-Score:-4.03|P-value:1.32E-4 ID:SGTC_3093|Compound:9117344|FD-Score:5.25|P-value:3.40E-7 ID:SGTC_3094|Compound:9117742|FD-Score:3.81|P-value:1.42E-4 ID:SGTC_3107|Compound:9121808|FD-Score:-3.85|P-value:2.51E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.81|P-value:1.43E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.50|P-value:4.18E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.56|P-value:3.39E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.09|P-value:5.13E-5 ID:SGTC_3283|Compound:9109332|FD-Score:5.47|P-value:1.17E-7 ID:SGTC_3290|Compound:9121031|FD-Score:4.41|P-value:1.40E-5 ID:SGTC_3294|Compound:9122953|FD-Score:4.67|P-value:4.84E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.58|P-value:3.16E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.62|P-value:2.81E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.54|P-value:3.62E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.54|P-value:8.25E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.34|P-value:7.17E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.57|P-value:7.33E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:7.37|P-value:2.09E-12 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.57|P-value:6.47E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.77|P-value:3.16E-6 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.28|P-value:3.04E-7 ID:SGTC_264|Compound:promethazine|FD-Score:11.90|P-value:5.25E-29 ID:SGTC_289|Compound:k017-0060|FD-Score:3.29|P-value:8.25E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.35|P-value:6.84E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.53|P-value:3.77E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.46|P-value:4.83E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:5.18|P-value:4.72E-7 ID:SGTC_438|Compound:sphingosine|FD-Score:4.46|P-value:1.17E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.94|P-value:3.30E-11 ID:SGTC_512|Compound:prima-1|FD-Score:4.01|P-value:6.82E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.95|P-value:1.74E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.93|P-value:9.26E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:3.50|P-value:4.17E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.35|P-value:6.79E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:3.55|P-value:3.58E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.41|P-value:5.54E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.58|P-value:3.24E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.52|P-value:3.91E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.28|P-value:8.68E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.83|P-value:1.32E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.43|P-value:5.22E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.08|P-value:5.27E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.80|P-value:1.47E-4 ID:SGTC_1746|Compound:st038117|FD-Score:5.33|P-value:2.30E-7 ID:SGTC_1807|Compound:st050092|FD-Score:3.34|P-value:7.18E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.25|P-value:9.49E-4 ID:SGTC_1905|Compound:st060207|FD-Score:-3.46|P-value:9.16E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.31|P-value:2.56E-7 ID:SGTC_1947|Compound:st076332|FD-Score:4.27|P-value:2.46E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.23|P-value:2.90E-5 ID:SGTC_2289|Compound:7946404|FD-Score:6.01|P-value:7.07E-9 ID:SGTC_2402|Compound:5553605|FD-Score:6.16|P-value:3.22E-9 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.40|P-value:5.75E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:4.66|P-value:5.04E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.41|P-value:5.59E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.92|P-value:9.56E-5 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.31|P-value:7.85E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.96|P-value:1.31E-6 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.24|P-value:9.83E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:4.52|P-value:8.99E-6 ID:SGTC_2740|Compound:felodipine|FD-Score:3.55|P-value:3.58E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.48|P-value:1.08E-5 ID:SGTC_2982|Compound:9019404|FD-Score:3.44|P-value:5.16E-4 ID:SGTC_2994|Compound:9053520|FD-Score:-4.03|P-value:1.32E-4 ID:SGTC_3093|Compound:9117344|FD-Score:5.25|P-value:3.40E-7 ID:SGTC_3094|Compound:9117742|FD-Score:3.81|P-value:1.42E-4 ID:SGTC_3107|Compound:9121808|FD-Score:-3.85|P-value:2.51E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.81|P-value:1.43E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.50|P-value:4.18E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.56|P-value:3.39E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.09|P-value:5.13E-5 ID:SGTC_3283|Compound:9109332|FD-Score:5.47|P-value:1.17E-7 ID:SGTC_3290|Compound:9121031|FD-Score:4.41|P-value:1.40E-5 ID:SGTC_3294|Compound:9122953|FD-Score:4.67|P-value:4.84E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.58|P-value:3.16E-4

Top fitness defect scores for YPL157W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 11.90 12.5 mM 5.25E-29 DNA intercalators 7 7.67E-33
2 SGTC_145 3737-0113 7.37 38.4 µM 2.09E-12 DNA intercalators 13 8.58E-14
3 SGTC_471 1-acyl-paf 6.94 4.0 µM 3.30E-11 DNA intercalators 2 2.01E-12
4 SGTC_2402 5553605 6.16 200.0 µM 3.22E-9 4 3.73E-10
5 SGTC_2289 7946404 6.01 165.1 µM 7.07E-9 37 9.15E-10
6 SGTC_3283 9109332 5.47 35.8 µM 1.17E-7 DNA intercalators 2 2.24E-8
7 SGTC_1746 st038117 5.33 42.0 µM 2.30E-7 11 4.80E-8
8 SGTC_1926 methyl fluorone black 5.31 53.6 µM 2.56E-7 DNA intercalators 28 5.43E-8
9 SGTC_257 daunorubicin 5.28 18.5 µM 3.04E-7 anthracycline transcription coupled DNA repair 14 6.60E-8
10 SGTC_3093 9117344 5.25 49.5 µM 3.40E-7 60S ribosome export 5 7.50E-8
11 SGTC_407 eburnamonine 5.18 100.0 µM 4.72E-7 5 1.09E-7
12 SGTC_2638 epoxomicin 4.96 18.0 µM 1.31E-6 11 3.47E-7
13 SGTC_230 MPP+ 4.77 141.7 µM 3.16E-6 8 9.40E-7
14 SGTC_3294 9122953 4.67 26.3 µM 4.84E-6 tubulin folding & SWR complex 15 1.52E-6
15 SGTC_2555 cholic acid 4.66 100.0 µM 5.04E-6 10 1.59E-6
16 SGTC_144 4161-2749 4.57 112.4 µM 7.33E-6 DNA intercalators 17 2.43E-6
17 SGTC_77 0828-0286 4.54 12.5 µM 8.25E-6 TSC3-RPN4 19 2.78E-6
18 SGTC_2722 naftopidil 4.52 47.2 µM 8.99E-6 4 3.06E-6
19 SGTC_2761 levamisole 4.48 97.9 µM 1.08E-5 8 3.78E-6
20 SGTC_438 sphingosine 4.46 6.7 µM 1.17E-5 14 4.11E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.388 7.22E-121 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.375 2.69E-112 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.365 6.25E-106 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.343 3.04E-93 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.341 2.19E-92 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.341 7.58E-92 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.332 9.00E-87 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.278 2.25E-60 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.275 4.19E-59 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.268 3.64E-56 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.250 8.42E-49 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.238 1.44E-44 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.233 1.08E-42 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.218 1.44E-37 YBR170C NPL4 hom Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
0.215 2.22E-36 YHR037W PUT2 hom Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism