YPL158C / AIM44

Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL158C / AIM44

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:-3.82|P-value:1.71E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.71|P-value:4.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:8.67|P-value:1.74E-15 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.42|P-value:6.55E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:4.48|P-value:2.76E-5 ID:SGTC_427|Compound:1354-0053|FD-Score:-4.16|P-value:4.95E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.44|P-value:3.19E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.80|P-value:7.58E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:4.52|P-value:2.32E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:4.25|P-value:6.69E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:-3.69|P-value:2.66E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:4.02|P-value:1.52E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:4.71|P-value:1.10E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.55|P-value:7.45E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-4.10|P-value:6.15E-5 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.81|P-value:3.11E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.77|P-value:2.00E-4 ID:SGTC_1642|Compound:st009582|FD-Score:6.30|P-value:5.86E-9 ID:SGTC_1933|Compound:st074711|FD-Score:4.29|P-value:5.62E-5 ID:SGTC_2137|Compound:5261020|FD-Score:5.92|P-value:4.25E-8 ID:SGTC_2147|Compound:5545932|FD-Score:4.04|P-value:1.44E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.35|P-value:8.16E-4 ID:SGTC_2394|Compound:5221648|FD-Score:5.45|P-value:4.29E-7 ID:SGTC_2401|Compound:5128683|FD-Score:5.00|P-value:3.22E-6 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:5.72|P-value:1.17E-7 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:6.06|P-value:2.05E-8 ID:SGTC_2507|Compound:purpurin|FD-Score:5.84|P-value:6.58E-8 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.69|P-value:4.73E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.88|P-value:1.38E-4 ID:SGTC_2585|Compound:alliin|FD-Score:4.41|P-value:3.68E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.90|P-value:2.30E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.74|P-value:9.56E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.29|P-value:5.60E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.83|P-value:2.97E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.44|P-value:3.24E-5 ID:SGTC_2832|Compound:7992762|FD-Score:3.68|P-value:4.87E-4 ID:SGTC_3020|Compound:9089140|FD-Score:3.56|P-value:7.15E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.76|P-value:3.69E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:-3.82|P-value:1.71E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.71|P-value:4.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:8.67|P-value:1.74E-15 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.42|P-value:6.55E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:4.48|P-value:2.76E-5 ID:SGTC_427|Compound:1354-0053|FD-Score:-4.16|P-value:4.95E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.44|P-value:3.19E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.80|P-value:7.58E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:4.52|P-value:2.32E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:4.25|P-value:6.69E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:-3.69|P-value:2.66E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:4.02|P-value:1.52E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:4.71|P-value:1.10E-5 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.55|P-value:7.45E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-4.10|P-value:6.15E-5 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.81|P-value:3.11E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.77|P-value:2.00E-4 ID:SGTC_1642|Compound:st009582|FD-Score:6.30|P-value:5.86E-9 ID:SGTC_1933|Compound:st074711|FD-Score:4.29|P-value:5.62E-5 ID:SGTC_2137|Compound:5261020|FD-Score:5.92|P-value:4.25E-8 ID:SGTC_2147|Compound:5545932|FD-Score:4.04|P-value:1.44E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.35|P-value:8.16E-4 ID:SGTC_2394|Compound:5221648|FD-Score:5.45|P-value:4.29E-7 ID:SGTC_2401|Compound:5128683|FD-Score:5.00|P-value:3.22E-6 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:5.72|P-value:1.17E-7 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:6.06|P-value:2.05E-8 ID:SGTC_2507|Compound:purpurin|FD-Score:5.84|P-value:6.58E-8 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.69|P-value:4.73E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.88|P-value:1.38E-4 ID:SGTC_2585|Compound:alliin|FD-Score:4.41|P-value:3.68E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.90|P-value:2.30E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.74|P-value:9.56E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.29|P-value:5.60E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.83|P-value:2.97E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.44|P-value:3.24E-5 ID:SGTC_2832|Compound:7992762|FD-Score:3.68|P-value:4.87E-4 ID:SGTC_3020|Compound:9089140|FD-Score:3.56|P-value:7.15E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.76|P-value:3.69E-4

Top fitness defect scores for YPL158C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 8.67 9.0 µM 1.74E-15 cell wall 107 2.24E-18
2 SGTC_1642 st009582 6.30 77.7 µM 5.86E-9 RPP1 & pyrimidine depletion 3 1.47E-10
3 SGTC_2503 beta-caryophyllene alcohol 6.06 24.7 µM 2.05E-8 2 6.60E-10
4 SGTC_2137 5261020 5.92 140.0 µM 4.25E-8 13 1.58E-9
5 SGTC_2507 purpurin 5.84 79.7 µM 6.58E-8 copper-dependent oxidative stress 12 2.67E-9
6 SGTC_2497 7-hydroxyflavone 5.72 100.0 µM 1.17E-7 4 5.33E-9
7 SGTC_2394 5221648 5.45 200.0 µM 4.29E-7 7 2.53E-8
8 SGTC_2401 5128683 5.00 200.0 µM 3.22E-6 14 2.84E-7
9 SGTC_574 1315-0397 4.80 40.0 µM 7.58E-6 14 7.93E-7
10 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 4.74 100.0 µM 9.56E-6 60S ribosome export 11 1.05E-6
11 SGTC_1100 amiodarone 4.71 5.1 µM 1.10E-5 5 1.24E-6
12 SGTC_666 0272-0154 4.52 136.0 µM 2.32E-5 cell wall 39 3.03E-6
13 SGTC_272 doxorubicin 4.48 8.1 µM 2.76E-5 anthracycline transcription coupled DNA repair 14 3.74E-6
14 SGTC_572 0960-0087 4.44 17.7 µM 3.19E-5 18 4.44E-6
15 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.44 100.0 µM 3.24E-5 25 4.52E-6
16 SGTC_2585 alliin 4.41 100.0 µM 3.68E-5 16 5.27E-6
17 SGTC_2604 4'-hydroxychalcone 4.29 11.3 µM 5.60E-5 26 8.74E-6
18 SGTC_1933 st074711 4.29 15.9 µM 5.62E-5 31 8.77E-6
19 SGTC_667 1498-1044 4.25 59.0 µM 6.69E-5 RPP1 & pyrimidine depletion 12 1.08E-5
20 SGTC_2147 5545932 4.04 89.7 µM 1.44E-4 ergosterol biosynthesis 10 2.71E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.191 6.21E-29 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.178 2.08E-25 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.177 5.92E-25 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.161 6.37E-21 YBR292C_d YBR292C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
0.160 1.26E-20 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.156 8.65E-20 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.156 1.12E-19 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.153 4.56E-19 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.151 1.50E-18 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.149 4.16E-18 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.149 4.52E-18 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.147 1.30E-17 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.145 3.11E-17 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.136 2.68E-15 YJR009C TDH2 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication
0.133 1.25E-14 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress