YPL164C / MLH3

Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL164C / MLH3

Click on Significant Values for Screen Details ID:SGTC_98|Compound:0074-0023|FD-Score:-3.28|P-value:4.44E-4 ID:SGTC_229|Compound:benomyl|FD-Score:-3.12|P-value:7.71E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.48|P-value:2.20E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:3.37|P-value:3.38E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.13|P-value:7.85E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.92|P-value:3.43E-7 ID:SGTC_829|Compound:1635-0120|FD-Score:3.80|P-value:6.33E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:4.01|P-value:2.52E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:3.83|P-value:5.55E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:4.20|P-value:1.13E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.87|P-value:4.63E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.43|P-value:2.64E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.12|P-value:8.18E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.43|P-value:2.56E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:5.51|P-value:1.29E-8 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.28|P-value:4.74E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.80|P-value:5.93E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.14|P-value:1.03E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:-5.01|P-value:1.93E-7 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.30|P-value:4.33E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-3.75|P-value:7.14E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-4.31|P-value:6.22E-6 ID:SGTC_1650|Compound:st011916|FD-Score:3.71|P-value:9.06E-5 ID:SGTC_1658|Compound:st012944|FD-Score:3.23|P-value:5.57E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.24|P-value:5.41E-4 ID:SGTC_1706|Compound:st031321|FD-Score:-3.35|P-value:3.40E-4 ID:SGTC_1742|Compound:st038235|FD-Score:7.09|P-value:3.94E-13 ID:SGTC_1772|Compound:st048439|FD-Score:3.27|P-value:4.83E-4 ID:SGTC_1909|Compound:st060837|FD-Score:3.29|P-value:4.44E-4 ID:SGTC_1966|Compound:st070145|FD-Score:5.89|P-value:1.36E-9 ID:SGTC_1971|Compound:st069429|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.11|P-value:8.40E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.53|P-value:1.81E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.10|P-value:1.70E-5 ID:SGTC_2486|Compound:5283202|FD-Score:3.44|P-value:2.58E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.75|P-value:7.69E-5 ID:SGTC_2726|Compound:cimetidine|FD-Score:3.17|P-value:6.91E-4 ID:SGTC_2744|Compound:captopril|FD-Score:-3.11|P-value:8.20E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:4.22|P-value:1.01E-5 ID:SGTC_2917|Compound:7979103|FD-Score:-3.08|P-value:9.12E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.61|P-value:1.35E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:-3.28|P-value:4.44E-4 ID:SGTC_229|Compound:benomyl|FD-Score:-3.12|P-value:7.71E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.48|P-value:2.20E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:3.37|P-value:3.38E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.13|P-value:7.85E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.92|P-value:3.43E-7 ID:SGTC_829|Compound:1635-0120|FD-Score:3.80|P-value:6.33E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:4.01|P-value:2.52E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:3.83|P-value:5.55E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:4.20|P-value:1.13E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.87|P-value:4.63E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.43|P-value:2.64E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.12|P-value:8.18E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.43|P-value:2.56E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:5.51|P-value:1.29E-8 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.28|P-value:4.74E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.80|P-value:5.93E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.14|P-value:1.03E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:-5.01|P-value:1.93E-7 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.30|P-value:4.33E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-3.75|P-value:7.14E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-4.31|P-value:6.22E-6 ID:SGTC_1650|Compound:st011916|FD-Score:3.71|P-value:9.06E-5 ID:SGTC_1658|Compound:st012944|FD-Score:3.23|P-value:5.57E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.24|P-value:5.41E-4 ID:SGTC_1706|Compound:st031321|FD-Score:-3.35|P-value:3.40E-4 ID:SGTC_1742|Compound:st038235|FD-Score:7.09|P-value:3.94E-13 ID:SGTC_1772|Compound:st048439|FD-Score:3.27|P-value:4.83E-4 ID:SGTC_1909|Compound:st060837|FD-Score:3.29|P-value:4.44E-4 ID:SGTC_1966|Compound:st070145|FD-Score:5.89|P-value:1.36E-9 ID:SGTC_1971|Compound:st069429|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.11|P-value:8.40E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.53|P-value:1.81E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.10|P-value:1.70E-5 ID:SGTC_2486|Compound:5283202|FD-Score:3.44|P-value:2.58E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.75|P-value:7.69E-5 ID:SGTC_2726|Compound:cimetidine|FD-Score:3.17|P-value:6.91E-4 ID:SGTC_2744|Compound:captopril|FD-Score:-3.11|P-value:8.20E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:4.22|P-value:1.01E-5 ID:SGTC_2917|Compound:7979103|FD-Score:-3.08|P-value:9.12E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.61|P-value:1.35E-4

Top fitness defect scores for YPL164C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1742 st038235 7.09 28.9 µM 3.94E-13 mitochondrial stress 27 6.77E-13
2 SGTC_1966 st070145 5.89 16.1 µM 1.36E-9 7 1.96E-9
3 SGTC_1205 4161-2785 5.51 76.0 µM 1.29E-8 9 1.78E-8
4 SGTC_1244 0416-0015 5.14 52.5 µM 1.03E-7 ERG2 12 1.37E-7
5 SGTC_781 4335-2806 4.92 94.4 µM 3.43E-7 8 4.43E-7
6 SGTC_2749 bexarotene 4.22 57.4 µM 1.01E-5 15 1.21E-5
7 SGTC_1007 4101-0031 4.20 70.0 µM 1.13E-5 14 1.35E-5
8 SGTC_2476 5806916 4.10 10.5 µM 1.70E-5 12 2.03E-5
9 SGTC_834 0312-0008 4.01 75.7 µM 2.52E-5 NEO1 32 2.98E-5
10 SGTC_1103 duloxetine 3.87 36.1 µM 4.63E-5 33 5.40E-5
11 SGTC_878 0828-0289 3.83 13.7 µM 5.55E-5 51 6.46E-5
12 SGTC_829 1635-0120 3.80 117.0 µM 6.33E-5 DNA damage response 46 7.35E-5
13 SGTC_2505 galangin 3.75 7.9 µM 7.69E-5 RPP1 & pyrimidine depletion 13 8.89E-5
14 SGTC_1650 st011916 3.71 45.8 µM 9.06E-5 17 1.04E-4
15 SGTC_3149 9097855 3.61 49.5 µM 1.35E-4 Golgi 36 1.54E-4
16 SGTC_2064 5220758 3.53 200.0 µM 1.81E-4 52 2.06E-4
17 SGTC_342 k048-0088 3.48 23.3 µM 2.20E-4 RSC & ERG11 32 2.50E-4
18 SGTC_2486 5283202 3.44 22.4 µM 2.58E-4 57 2.91E-4
19 SGTC_1189 1431-2119 3.43 7.3 µM 2.64E-4 52 2.98E-4
20 SGTC_1971 st069429 3.39 63.5 µM 3.09E-4 60S ribosome export 25 3.48E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.69E-10 YCL024W KCC4 hom Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication
0.107 5.03E-10 YLR439W MRPL4 hom Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
0.103 1.95E-9 YJR057W CDC8 het Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
0.102 3.04E-9 YNR034W SOL1 hom Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.101 3.98E-9 YLR297W YLR297W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
0.098 1.41E-8 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.092 8.24E-8 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.090 1.77E-7 YML058W-A HUG1 hom Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress
0.088 3.03E-7 YPL220W RPL1A hom Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.088 3.21E-7 YLR149C YLR149C hom Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.082 1.86E-6 YGL130W CEG1 het Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
0.082 1.89E-6 YPR134W MSS18 hom Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
0.082 2.24E-6 YNL090W RHO2 hom Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
0.081 2.90E-6 YDR489W SLD5 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.078 5.43E-6 YFR054C_d YFR054C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data