YPL175W / SPT14

UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL175W / SPT14

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:5.04|P-value:2.96E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:3.96|P-value:1.89E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.34|P-value:7.99E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:8.08|P-value:2.02E-13 ID:SGTC_137|Compound:0438-0332|FD-Score:5.15|P-value:1.86E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:4.51|P-value:2.61E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.63|P-value:5.55E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:4.64|P-value:1.52E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:5.14|P-value:1.94E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.60|P-value:6.21E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.63|P-value:5.61E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.64|P-value:5.44E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.03|P-value:1.48E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.23|P-value:7.22E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:-5.07|P-value:2.63E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:5.98|P-value:4.01E-8 ID:SGTC_362|Compound:0310-0100|FD-Score:-4.52|P-value:2.42E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-6.03|P-value:3.02E-8 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.59|P-value:6.32E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:10.50|P-value:3.10E-21 ID:SGTC_372|Compound:0155-0182|FD-Score:3.57|P-value:6.68E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.83|P-value:2.97E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.80|P-value:3.26E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.69|P-value:1.28E-5 ID:SGTC_512|Compound:prima-1|FD-Score:6.33|P-value:6.66E-9 ID:SGTC_525|Compound:k781-2675|FD-Score:3.52|P-value:7.93E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-4.14|P-value:9.98E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.51|P-value:2.55E-5 ID:SGTC_716|Compound:1683-5115|FD-Score:4.87|P-value:6.09E-6 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.57|P-value:6.64E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.56|P-value:6.90E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.50|P-value:2.65E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.68|P-value:1.32E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.97|P-value:1.80E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.85|P-value:2.76E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:4.20|P-value:8.11E-5 ID:SGTC_1522|Compound:st012842|FD-Score:5.04|P-value:3.00E-6 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.51|P-value:8.24E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.94|P-value:2.05E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-3.49|P-value:8.65E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-7.93|P-value:5.04E-13 ID:SGTC_1635|Compound:st007035|FD-Score:5.41|P-value:5.83E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.51|P-value:2.54E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.93|P-value:4.75E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.86|P-value:2.64E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.98|P-value:1.75E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.56|P-value:2.12E-5 ID:SGTC_2404|Compound:5161160|FD-Score:4.24|P-value:7.12E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.46|P-value:9.46E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:5.32|P-value:8.95E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.81|P-value:3.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.81|P-value:7.81E-6 ID:SGTC_2780|Compound:5215298|FD-Score:4.27|P-value:6.32E-5 ID:SGTC_2784|Compound:7567673|FD-Score:3.80|P-value:3.27E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.69|P-value:4.66E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:5.04|P-value:2.96E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:3.96|P-value:1.89E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.34|P-value:7.99E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:8.08|P-value:2.02E-13 ID:SGTC_137|Compound:0438-0332|FD-Score:5.15|P-value:1.86E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:4.51|P-value:2.61E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.63|P-value:5.55E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:4.64|P-value:1.52E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:5.14|P-value:1.94E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.60|P-value:6.21E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.63|P-value:5.61E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.64|P-value:5.44E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.03|P-value:1.48E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:4.23|P-value:7.22E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:-5.07|P-value:2.63E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:5.98|P-value:4.01E-8 ID:SGTC_362|Compound:0310-0100|FD-Score:-4.52|P-value:2.42E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-6.03|P-value:3.02E-8 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.59|P-value:6.32E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:10.50|P-value:3.10E-21 ID:SGTC_372|Compound:0155-0182|FD-Score:3.57|P-value:6.68E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.83|P-value:2.97E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.80|P-value:3.26E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.69|P-value:1.28E-5 ID:SGTC_512|Compound:prima-1|FD-Score:6.33|P-value:6.66E-9 ID:SGTC_525|Compound:k781-2675|FD-Score:3.52|P-value:7.93E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-4.14|P-value:9.98E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.51|P-value:2.55E-5 ID:SGTC_716|Compound:1683-5115|FD-Score:4.87|P-value:6.09E-6 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.57|P-value:6.64E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.56|P-value:6.90E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.50|P-value:2.65E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.68|P-value:1.32E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.97|P-value:1.80E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.85|P-value:2.76E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:4.20|P-value:8.11E-5 ID:SGTC_1522|Compound:st012842|FD-Score:5.04|P-value:3.00E-6 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.51|P-value:8.24E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.94|P-value:2.05E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-3.49|P-value:8.65E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-7.93|P-value:5.04E-13 ID:SGTC_1635|Compound:st007035|FD-Score:5.41|P-value:5.83E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.51|P-value:2.54E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.93|P-value:4.75E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.86|P-value:2.64E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.98|P-value:1.75E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.56|P-value:2.12E-5 ID:SGTC_2404|Compound:5161160|FD-Score:4.24|P-value:7.12E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.46|P-value:9.46E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:5.32|P-value:8.95E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.81|P-value:3.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.81|P-value:7.81E-6 ID:SGTC_2780|Compound:5215298|FD-Score:4.27|P-value:6.32E-5 ID:SGTC_2784|Compound:7567673|FD-Score:3.80|P-value:3.27E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.69|P-value:4.66E-4

Top fitness defect scores for YPL175W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_367 0335-0881 10.50 7.1 µM 3.10E-21 ubiquinone biosynthesis & proteasome 4 7.27E-26 2.35
2 SGTC_64 000l-0018 8.08 272.1 µM 2.02E-13 ubiquinone biosynthesis & proteasome 18 3.33E-16 0.06
3 SGTC_512 prima-1 6.33 270.0 µM 6.66E-9 DNA intercalators 2 1.25E-10 0.78
4 SGTC_361 000s-0510 5.98 1.4 µM 4.01E-8 2 1.14E-9 0.46
5 SGTC_1635 st007035 5.41 42.4 µM 5.83E-7 9 3.09E-8 0.01
6 SGTC_36 0335-0881 5.34 14.2 µM 7.99E-7 ubiquinone biosynthesis & proteasome 18 4.56E-8 0.04
7 SGTC_2499 avocadenofuran 5.32 51.5 µM 8.95E-7 1 5.24E-8 0.91
8 SGTC_137 0438-0332 5.15 85.4 µM 1.86E-6 ubiquinone biosynthesis & proteasome 26 1.29E-7 0.02
9 SGTC_227 fluconazole 5.14 33.5 µM 1.94E-6 azole & statin 34 1.36E-7 0.05
10 SGTC_9 0092-0002 5.04 10.4 µM 2.96E-6 2 2.28E-7 0.04
11 SGTC_1522 st012842 5.04 45.6 µM 3.00E-6 RNA pol III & RNase P/MRP 6 2.32E-7 0.14
12 SGTC_1926 methyl fluorone black 4.93 53.6 µM 4.75E-6 DNA intercalators 19 4.08E-7 0.14
13 SGTC_716 1683-5115 4.87 658.0 µM 6.09E-6 1 5.54E-7 0.80
14 SGTC_2679 rapamycin 4.81 1.0 nM 7.81E-6 42 7.52E-7 0.02
15 SGTC_471 1-acyl-paf 4.69 4.0 µM 1.28E-5 DNA intercalators 12 1.38E-6 0.09
16 SGTC_1173 0443-0020 4.68 122.0 µM 1.32E-5 TSC3-RPN4 2 1.44E-6 0.46
17 SGTC_216 k038-0035 4.64 28.4 µM 1.52E-5 ubiquinone biosynthesis & proteasome 11 1.71E-6 0.17
18 SGTC_2363 9053361 4.56 176.0 µM 2.12E-5 Golgi 8 2.57E-6 0.23
19 SGTC_1844 rapamycin 4.51 4.0 nM 2.54E-5 41 3.21E-6 0.01
20 SGTC_145 3737-0113 4.51 38.4 µM 2.61E-5 DNA intercalators 38 3.32E-6 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 6.78E-38 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.212 3.02E-35 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.210 1.15E-34 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.206 3.07E-33 YPL124W SPC29 het Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
0.201 1.14E-31 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.196 2.84E-30 YPL165C SET6 hom SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
0.189 2.77E-28 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.188 5.05E-28 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.187 8.83E-28 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187 9.78E-28 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.187 1.25E-27 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184 8.56E-27 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.182 2.08E-26 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.179 2.11E-25 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.178 2.99E-25 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation