HIPHOP chemogenomics database

YPL175W / SPT14

UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

Zygosity: Heterozygous strain

fixedexpanded
Profile for YPL175W / SPT14

Click on Significant Values for Screen Details

Top fitness defect scores for YPL175W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_367	0335-0881	10.50	7.1 µM	3.10E-21	ubiquinone biosynthesis & proteasome	4	7.27E-26	2.35
2	SGTC_64	000l-0018	8.08	272.1 µM	2.02E-13	ubiquinone biosynthesis & proteasome	18	3.33E-16	0.06
3	SGTC_512	prima-1	6.33	270.0 µM	6.66E-9	DNA intercalators	2	1.25E-10	0.78
4	SGTC_361	000s-0510	5.98	1.4 µM	4.01E-8		2	1.14E-9	0.46
5	SGTC_1635	st007035	5.41	42.4 µM	5.83E-7		9	3.09E-8	0.01
6	SGTC_36	0335-0881	5.34	14.2 µM	7.99E-7	ubiquinone biosynthesis & proteasome	18	4.56E-8	0.04
7	SGTC_2499	avocadenofuran	5.32	51.5 µM	8.95E-7		1	5.24E-8	0.91
8	SGTC_137	0438-0332	5.15	85.4 µM	1.86E-6	ubiquinone biosynthesis & proteasome	26	1.29E-7	0.02
9	SGTC_227	fluconazole	5.14	33.5 µM	1.94E-6	azole & statin	34	1.36E-7	0.05
10	SGTC_9	0092-0002	5.04	10.4 µM	2.96E-6		2	2.28E-7	0.04
11	SGTC_1522	st012842	5.04	45.6 µM	3.00E-6	RNA pol III & RNase P/MRP	6	2.32E-7	0.14
12	SGTC_1926	methyl fluorone black	4.93	53.6 µM	4.75E-6	DNA intercalators	19	4.08E-7	0.14
13	SGTC_716	1683-5115	4.87	658.0 µM	6.09E-6		1	5.54E-7	0.80
14	SGTC_2679	rapamycin	4.81	1.0 nM	7.81E-6		42	7.52E-7	0.02
15	SGTC_471	1-acyl-paf	4.69	4.0 µM	1.28E-5	DNA intercalators	12	1.38E-6	0.09
16	SGTC_1173	0443-0020	4.68	122.0 µM	1.32E-5	TSC3-RPN4	2	1.44E-6	0.46
17	SGTC_216	k038-0035	4.64	28.4 µM	1.52E-5	ubiquinone biosynthesis & proteasome	11	1.71E-6	0.17
18	SGTC_2363	9053361	4.56	176.0 µM	2.12E-5	Golgi	8	2.57E-6	0.23
19	SGTC_1844	rapamycin	4.51	4.0 nM	2.54E-5		41	3.21E-6	0.01
20	SGTC_145	3737-0113	4.51	38.4 µM	2.61E-5	DNA intercalators	38	3.32E-6	0.07

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.220	6.78E-38	YGL119W	COQ8	hom	Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.212	3.02E-35	YNR042W_d	YNR042W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.210	1.15E-34	YGR156W	PTI1	het	Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.206	3.07E-33	YPL124W	SPC29	het	Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
0.201	1.14E-31	YHR036W	BRL1	het	Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.196	2.84E-30	YPL165C	SET6	hom	SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
0.189	2.77E-28	YGR255C	COQ6	hom	Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.188	5.05E-28	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.187	8.83E-28	YPR146C_d	YPR146C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187	9.78E-28	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.187	1.25E-27	YML034C-A_d	YML034C-A_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184	8.56E-27	YJL054W	TIM54	het	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.182	2.08E-26	YOR125C	CAT5	hom	Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.179	2.11E-25	YMR067C	UBX4	hom	UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.178	2.99E-25	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation