HIPHOP chemogenomics database

YPL202C / AFT2

Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL202C / AFT2

Click on Significant Values for Screen Details

Top fitness defect scores for YPL202C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1800	st050079	11.10	29.1 µM	8.33E-30	NEO1-PIK1	1	3.60E-29
2	SGTC_1801	st050089	10.60	28.4 µM	4.45E-27	NEO1-PIK1	11	1.77E-26
3	SGTC_467	ro 31-8220	7.34	72.8 µM	4.33E-14	calcium & mitochondrial duress	18	1.08E-13
4	SGTC_1844	rapamycin	6.92	4.0 nM	9.27E-13		73	2.20E-12
5	SGTC_221	trifluoperazine	6.91	10.3 µM	1.06E-12	NEO1-PIK1	103	2.50E-12
6	SGTC_1803	st050068	6.56	52.4 µM	1.18E-11	NEO1-PIK1	64	2.67E-11
7	SGTC_1760	st045453	6.46	10.4 µM	2.41E-11	NEO1-PIK1	74	5.36E-11
8	SGTC_1035	k081-0041	6.16	55.2 µM	1.71E-10	Golgi	4	3.67E-10
9	SGTC_1818	st052136	5.82	56.3 µM	1.46E-9	NEO1-PIK1	14	2.99E-9
10	SGTC_1724	st037281	5.50	8.3 µM	9.74E-9		13	1.92E-8
11	SGTC_1754	st042065	5.13	16.1 µM	7.70E-8	NEO1-PIK1	121	1.45E-7
12	SGTC_2415	st077333	4.91	57.7 µM	2.49E-7		20	4.56E-7
13	SGTC_475	α-linolenic acid	4.89	10.0 µM	2.74E-7	excess fatty acid	8	4.99E-7
14	SGTC_1530	rhein	4.87	70.4 µM	3.01E-7		9	5.48E-7
15	SGTC_21	0849-0113	4.74	23.0 µM	6.04E-7		14	1.08E-6
16	SGTC_2679	rapamycin	4.65	1.0 nM	9.36E-7		109	1.66E-6
17	SGTC_1873	rapamycin	4.61	2.0 nM	1.14E-6		56	2.01E-6
18	SGTC_1033	k072-0102	4.47	21.3 µM	2.30E-6	60S ribosome export	9	3.99E-6
19	SGTC_1715	st032160	4.27	10.2 µM	5.76E-6		38	9.75E-6
20	SGTC_722	3534-0461	4.16	190.0 µM	9.56E-6	PDR1	30	1.60E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.364	1.20E-105	YOR357C	SNX3	hom	Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.338	3.12E-90	YLR020C	YEH2	hom	Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.326	6.71E-84	YDR492W	IZH1	hom	Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.314	1.50E-77	YML097C	VPS9	hom	A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.309	4.02E-75	YJL052W	TDH1	hom	Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.309	4.64E-75	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.305	4.07E-73	YHR108W	GGA2	hom	Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.301	5.04E-71	YBR246W	RRT2	hom	Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.300	1.02E-70	YAR042W	SWH1	hom	Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.297	5.65E-69	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.294	9.79E-68	YOR115C	TRS33	hom	One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.293	2.44E-67	YGR166W	TRS65	hom	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.288	3.64E-65	YPR091C	NVJ2	hom	Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.279	4.83E-61	YPR092W_d	YPR092W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.274	5.69E-59	YJL154C	VPS35	hom	Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p