YPL212C / PUS1

tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL212C / PUS1

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:7.45|P-value:3.42E-14 ID:SGTC_89|Compound:0302-0250|FD-Score:4.09|P-value:1.79E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:16.40|P-value:1.19E-60 ID:SGTC_259|Compound:usnic acid|FD-Score:3.49|P-value:2.05E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:8.09|P-value:2.21E-16 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.48|P-value:2.94E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.11|P-value:8.01E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.20|P-value:5.94E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.24|P-value:5.22E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:4.80|P-value:6.64E-7 ID:SGTC_843|Compound:0310-0051|FD-Score:3.40|P-value:2.87E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.12|P-value:7.90E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-4.05|P-value:3.12E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:-3.48|P-value:2.92E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:5.75|P-value:3.61E-9 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.78|P-value:9.49E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.23|P-value:5.29E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.92|P-value:5.27E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.97|P-value:4.37E-5 ID:SGTC_1814|Compound:st052110|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.24|P-value:1.37E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.80|P-value:8.46E-5 ID:SGTC_1909|Compound:st060837|FD-Score:-3.49|P-value:2.87E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.27|P-value:6.21E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.18|P-value:8.51E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.08|P-value:9.10E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.15|P-value:2.03E-5 ID:SGTC_2006|Compound:4011487|FD-Score:4.74|P-value:8.68E-7 ID:SGTC_2007|Compound:4011539|FD-Score:5.05|P-value:1.83E-7 ID:SGTC_2009|Compound:4012297|FD-Score:6.43|P-value:4.81E-11 ID:SGTC_2018|Compound:5133068|FD-Score:3.06|P-value:9.78E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.62|P-value:1.72E-4 ID:SGTC_2034|Compound:5197015|FD-Score:8.44|P-value:1.11E-17 ID:SGTC_2043|Compound:5101342|FD-Score:3.65|P-value:1.12E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.42|P-value:2.67E-4 ID:SGTC_2142|Compound:5622915|FD-Score:3.14|P-value:7.33E-4 ID:SGTC_2198|Compound:6589944|FD-Score:3.25|P-value:4.97E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.96|P-value:3.11E-5 ID:SGTC_2328|Compound:6201408|FD-Score:-3.17|P-value:8.70E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.17|P-value:6.69E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.35|P-value:3.43E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.23|P-value:5.31E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.58|P-value:1.49E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:4.68|P-value:1.17E-6 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:-3.63|P-value:1.69E-4 ID:SGTC_2744|Compound:captopril|FD-Score:5.21|P-value:7.67E-8 ID:SGTC_2746|Compound:daunorubicin|FD-Score:4.02|P-value:2.47E-5 ID:SGTC_2807|Compound:7945238|FD-Score:3.30|P-value:4.20E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.07|P-value:9.34E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.14|P-value:1.46E-5 ID:SGTC_2860|Compound:9025725|FD-Score:3.25|P-value:5.07E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.99|P-value:2.79E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-3.64|P-value:1.61E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-5.99|P-value:1.37E-9 ID:SGTC_2949|Compound:9072542|FD-Score:4.98|P-value:2.57E-7 ID:SGTC_3037|Compound:9091026|FD-Score:-4.59|P-value:2.70E-6 ID:SGTC_3038|Compound:9091290|FD-Score:-4.26|P-value:1.24E-5 ID:SGTC_3043|Compound:9092303|FD-Score:-3.36|P-value:4.49E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.45|P-value:3.24E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:7.45|P-value:3.42E-14 ID:SGTC_89|Compound:0302-0250|FD-Score:4.09|P-value:1.79E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:16.40|P-value:1.19E-60 ID:SGTC_259|Compound:usnic acid|FD-Score:3.49|P-value:2.05E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:8.09|P-value:2.21E-16 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.48|P-value:2.94E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.11|P-value:8.01E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.20|P-value:5.94E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.24|P-value:5.22E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:4.80|P-value:6.64E-7 ID:SGTC_843|Compound:0310-0051|FD-Score:3.40|P-value:2.87E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.12|P-value:7.90E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-4.05|P-value:3.12E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:-3.48|P-value:2.92E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:5.75|P-value:3.61E-9 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.78|P-value:9.49E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.23|P-value:5.29E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.92|P-value:5.27E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.97|P-value:4.37E-5 ID:SGTC_1814|Compound:st052110|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.24|P-value:1.37E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.80|P-value:8.46E-5 ID:SGTC_1909|Compound:st060837|FD-Score:-3.49|P-value:2.87E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.27|P-value:6.21E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.18|P-value:8.51E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.08|P-value:9.10E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.15|P-value:2.03E-5 ID:SGTC_2006|Compound:4011487|FD-Score:4.74|P-value:8.68E-7 ID:SGTC_2007|Compound:4011539|FD-Score:5.05|P-value:1.83E-7 ID:SGTC_2009|Compound:4012297|FD-Score:6.43|P-value:4.81E-11 ID:SGTC_2018|Compound:5133068|FD-Score:3.06|P-value:9.78E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.62|P-value:1.72E-4 ID:SGTC_2034|Compound:5197015|FD-Score:8.44|P-value:1.11E-17 ID:SGTC_2043|Compound:5101342|FD-Score:3.65|P-value:1.12E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.42|P-value:2.67E-4 ID:SGTC_2142|Compound:5622915|FD-Score:3.14|P-value:7.33E-4 ID:SGTC_2198|Compound:6589944|FD-Score:3.25|P-value:4.97E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.96|P-value:3.11E-5 ID:SGTC_2328|Compound:6201408|FD-Score:-3.17|P-value:8.70E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.17|P-value:6.69E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.35|P-value:3.43E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.23|P-value:5.31E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.58|P-value:1.49E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:4.68|P-value:1.17E-6 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:-3.63|P-value:1.69E-4 ID:SGTC_2744|Compound:captopril|FD-Score:5.21|P-value:7.67E-8 ID:SGTC_2746|Compound:daunorubicin|FD-Score:4.02|P-value:2.47E-5 ID:SGTC_2807|Compound:7945238|FD-Score:3.30|P-value:4.20E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.07|P-value:9.34E-4 ID:SGTC_2835|Compound:9006588|FD-Score:4.14|P-value:1.46E-5 ID:SGTC_2860|Compound:9025725|FD-Score:3.25|P-value:5.07E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.99|P-value:2.79E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-3.64|P-value:1.61E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-5.99|P-value:1.37E-9 ID:SGTC_2949|Compound:9072542|FD-Score:4.98|P-value:2.57E-7 ID:SGTC_3037|Compound:9091026|FD-Score:-4.59|P-value:2.70E-6 ID:SGTC_3038|Compound:9091290|FD-Score:-4.26|P-value:1.24E-5 ID:SGTC_3043|Compound:9092303|FD-Score:-3.36|P-value:4.49E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.45|P-value:3.24E-4

Top fitness defect scores for YPL212C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_129 0088-0017 16.40 176.6 µM 1.19E-60 4 2.16E-60
2 SGTC_2034 5197015 8.44 53.1 µM 1.11E-17 SWF1 & branched chain AA biosynthesis 2 1.55E-17
3 SGTC_318 1488-0629 8.09 67.2 µM 2.21E-16 copper-dependent oxidative stress 6 3.05E-16
4 SGTC_29 0299-0004 7.45 13.0 µM 3.42E-14 8 4.61E-14
5 SGTC_2009 4012297 6.43 12.1 µM 4.81E-11 cell wall signaling 21 6.25E-11
6 SGTC_1001 4048-4355 5.75 44.4 µM 3.61E-9 copper-dependent oxidative stress 12 4.58E-9
7 SGTC_2744 captopril 5.21 92.0 µM 7.67E-8 60S ribosome export 3 9.53E-8
8 SGTC_2007 4011539 5.05 71.8 µM 1.83E-7 cell wall signaling 33 2.26E-7
9 SGTC_2949 9072542 4.98 2.2 µM 2.57E-7 TSC3-RPN4 13 3.17E-7
10 SGTC_697 0508-1955 4.80 564.0 µM 6.64E-7 15 8.12E-7
11 SGTC_2006 4011487 4.74 200.0 µM 8.68E-7 endomembrane recycling 60 1.06E-6
12 SGTC_2720 fluspirilene 4.68 36.9 µM 1.17E-6 ERG2 7 1.43E-6
13 SGTC_2835 9006588 4.14 71.4 µM 1.46E-5 33 1.74E-5
14 SGTC_89 0302-0250 4.09 142.1 µM 1.79E-5 16 2.13E-5
15 SGTC_2746 daunorubicin 4.02 9.9 µM 2.47E-5 anthracycline transcription coupled DNA repair 1 2.93E-5
16 SGTC_2920 7982904 3.99 15.8 µM 2.79E-5 23 3.31E-5
17 SGTC_2310 7668677 3.96 200.0 µM 3.11E-5 29 3.68E-5
18 SGTC_2389 9091542 3.65 195.5 µM 1.11E-4 iron homeostasis 45 1.30E-4
19 SGTC_2043 5101342 3.65 149.0 µM 1.12E-4 SWF1 & branched chain AA biosynthesis 24 1.31E-4
20 SGTC_395 anisomycin 3.59 6.2 µM 1.39E-4 15 1.63E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 4.36E-38 YMR302C YME2 hom Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
0.149 3.70E-18 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.132 1.52E-14 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.121 1.92E-12 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.113 5.31E-11 YGL167C PMR1 hom High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
0.103 2.19E-9 YNL032W SIW14 hom Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
0.097 1.84E-8 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.096 2.31E-8 YDR276C PMP3 hom Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
0.096 2.89E-8 YGL026C TRP5 hom Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
0.095 3.43E-8 YNL144C_p YNL144C_p hom Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study
0.094 4.70E-8 YHR206W SKN7 hom Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
0.094 5.47E-8 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.093 6.32E-8 YDL173W PAR32 hom Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene
0.092 1.01E-7 YGR116W SPT6 het Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
0.091 1.12E-7 YBR197C YBR197C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication