YPL214C / THI6

Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL214C / THI6

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:-3.22|P-value:7.53E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.41|P-value:2.56E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:4.75|P-value:4.69E-7 ID:SGTC_253|Compound:dyclonine|FD-Score:3.06|P-value:6.99E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:4.22|P-value:6.09E-6 ID:SGTC_357|Compound:1325-0123|FD-Score:-3.49|P-value:2.84E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.23|P-value:7.12E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.16|P-value:4.86E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:4.34|P-value:3.51E-6 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.46|P-value:1.57E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.38|P-value:4.29E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.74|P-value:5.22E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:3.21|P-value:4.08E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:4.25|P-value:5.48E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.86|P-value:3.06E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:3.65|P-value:7.58E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.09|P-value:6.24E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:-5.36|P-value:4.50E-8 ID:SGTC_773|Compound:4358-1457|FD-Score:4.56|P-value:1.22E-6 ID:SGTC_975|Compound:1335-0046|FD-Score:-4.38|P-value:6.61E-6 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.33|P-value:5.08E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.24|P-value:6.92E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.82|P-value:3.73E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.14|P-value:9.79E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.31|P-value:2.85E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.04|P-value:7.57E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.59|P-value:9.67E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-4.25|P-value:1.21E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.58|P-value:1.01E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.39|P-value:2.11E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.49|P-value:1.44E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.17|P-value:4.74E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.14|P-value:9.74E-4 ID:SGTC_1690|Compound:st023502|FD-Score:4.41|P-value:2.52E-6 ID:SGTC_2045|Compound:5106652|FD-Score:3.46|P-value:1.58E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-3.35|P-value:4.77E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.70|P-value:5.97E-5 ID:SGTC_2126|Compound:5251174|FD-Score:5.59|P-value:4.31E-9 ID:SGTC_2153|Compound:5569715|FD-Score:-3.49|P-value:2.79E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.06|P-value:6.93E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.41|P-value:1.91E-4 ID:SGTC_2367|Compound:9070478|FD-Score:-3.28|P-value:6.04E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.29|P-value:5.89E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.90|P-value:2.15E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.10|P-value:6.00E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.05|P-value:7.16E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.18|P-value:8.72E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.38|P-value:2.14E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.15|P-value:9.68E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.77|P-value:9.34E-5 ID:SGTC_3101|Compound:9120531|FD-Score:2.99|P-value:9.07E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.79|P-value:4.14E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.03|P-value:7.73E-4 ID:SGTC_3295|Compound:9122362|FD-Score:4.48|P-value:1.78E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.22|P-value:7.53E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.41|P-value:2.56E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:4.75|P-value:4.69E-7 ID:SGTC_253|Compound:dyclonine|FD-Score:3.06|P-value:6.99E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:4.22|P-value:6.09E-6 ID:SGTC_357|Compound:1325-0123|FD-Score:-3.49|P-value:2.84E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.23|P-value:7.12E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.16|P-value:4.86E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:4.34|P-value:3.51E-6 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.46|P-value:1.57E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.38|P-value:4.29E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.74|P-value:5.22E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:3.21|P-value:4.08E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:4.25|P-value:5.48E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.86|P-value:3.06E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:3.65|P-value:7.58E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.09|P-value:6.24E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:-5.36|P-value:4.50E-8 ID:SGTC_773|Compound:4358-1457|FD-Score:4.56|P-value:1.22E-6 ID:SGTC_975|Compound:1335-0046|FD-Score:-4.38|P-value:6.61E-6 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.33|P-value:5.08E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.24|P-value:6.92E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.82|P-value:3.73E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.14|P-value:9.79E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.31|P-value:2.85E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.04|P-value:7.57E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.59|P-value:9.67E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-4.25|P-value:1.21E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.58|P-value:1.01E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.39|P-value:2.11E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.49|P-value:1.44E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.17|P-value:4.74E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.14|P-value:9.74E-4 ID:SGTC_1690|Compound:st023502|FD-Score:4.41|P-value:2.52E-6 ID:SGTC_2045|Compound:5106652|FD-Score:3.46|P-value:1.58E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-3.35|P-value:4.77E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.70|P-value:5.97E-5 ID:SGTC_2126|Compound:5251174|FD-Score:5.59|P-value:4.31E-9 ID:SGTC_2153|Compound:5569715|FD-Score:-3.49|P-value:2.79E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.06|P-value:6.93E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.41|P-value:1.91E-4 ID:SGTC_2367|Compound:9070478|FD-Score:-3.28|P-value:6.04E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.29|P-value:5.89E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.90|P-value:2.15E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.10|P-value:6.00E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.05|P-value:7.16E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.18|P-value:8.72E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.38|P-value:2.14E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.15|P-value:9.68E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.77|P-value:9.34E-5 ID:SGTC_3101|Compound:9120531|FD-Score:2.99|P-value:9.07E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.79|P-value:4.14E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.03|P-value:7.73E-4 ID:SGTC_3295|Compound:9122362|FD-Score:4.48|P-value:1.78E-6

Top fitness defect scores for YPL214C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2126 5251174 5.59 33.9 µM 4.31E-9 5 1.16E-8
2 SGTC_2536 dehydroabietamide 4.90 32.0 µM 2.15E-7 10 4.91E-7
3 SGTC_168 3970-0862 4.75 530.5 µM 4.69E-7 79 1.03E-6
4 SGTC_773 4358-1457 4.56 44.6 µM 1.22E-6 28 2.59E-6
5 SGTC_3295 9122362 4.48 53.6 µM 1.78E-6 8 3.70E-6
6 SGTC_1690 st023502 4.41 41.2 µM 2.52E-6 calcium & mitochondrial duress 5 5.15E-6
7 SGTC_159 3970-0959 4.41 50.0 µM 2.56E-6 59 5.23E-6
8 SGTC_369 0957-0393 4.34 4.5 µM 3.51E-6 cell wall signaling 17 7.08E-6
9 SGTC_529 4076-0302 4.25 30.3 µM 5.48E-6 mitochondrial processes 14 1.09E-5
10 SGTC_355 1311-2252 4.22 105.0 µM 6.09E-6 12 1.20E-5
11 SGTC_568 1193-0097 3.86 7.5 µM 3.06E-5 ERAD & cell cycle 158 5.59E-5
12 SGTC_994 3910-0338 3.82 138.0 µM 3.73E-5 calcium & mitochondrial duress 50 6.76E-5
13 SGTC_3133 9127736 3.79 49.5 µM 4.14E-5 23 7.47E-5
14 SGTC_449 xestospongin c 3.74 11.2 µM 5.22E-5 NEO1-PIK1 77 9.31E-5
15 SGTC_2056 5235370 3.70 200.0 µM 5.97E-5 53 1.06E-4
16 SGTC_632 0781-2353 3.65 4.7 µM 7.58E-5 Golgi 55 1.33E-4
17 SGTC_1305 1181-0477 3.59 144.0 µM 9.67E-5 23 1.68E-4
18 SGTC_1341 1486-1360 3.58 280.0 µM 1.01E-4 40 1.75E-4
19 SGTC_1457 k015-0032 3.49 57.3 µM 1.44E-4 53 2.46E-4
20 SGTC_410 α-linolenic acid 3.46 100.0 µM 1.57E-4 excess fatty acid 52 2.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.30E-16 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.132 1.46E-14 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.113 4.78E-11 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.113 5.51E-11 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.109 2.58E-10 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.103 2.53E-9 YOL049W GSH2 hom Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.096 2.39E-8 YLR037C PAU23 hom Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
0.093 5.91E-8 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.088 3.71E-7 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.087 4.75E-7 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.086 6.69E-7 YDR330W UBX5 hom UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p
0.085 8.02E-7 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
0.084 1.12E-6 YOR358W HAP5 hom Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
0.084 1.26E-6 YLR053C_p YLR053C_p hom Putative protein of unknown function
0.083 1.72E-6 YMR316C-B_d YMR316C-B_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data