YPL218W / SAR1

GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL218W / SAR1

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:-3.62|P-value:1.09E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.79|P-value:4.99E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.17|P-value:6.18E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:4.65|P-value:9.41E-7 ID:SGTC_393|Compound:acivicin|FD-Score:3.28|P-value:3.83E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-3.54|P-value:1.51E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.99|P-value:2.30E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-5.60|P-value:5.33E-9 ID:SGTC_628|Compound:1636-0253|FD-Score:3.15|P-value:6.05E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:4.89|P-value:2.71E-7 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.04|P-value:9.63E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.44|P-value:2.27E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.42|P-value:3.23E-6 ID:SGTC_1222|Compound:0366-0074|FD-Score:4.16|P-value:9.87E-6 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.04|P-value:9.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:-3.22|P-value:5.13E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.56|P-value:1.30E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:4.03|P-value:1.76E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.78|P-value:5.79E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.79|P-value:4.95E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.33|P-value:3.11E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.33|P-value:3.16E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.19|P-value:5.85E-8 ID:SGTC_1887|Compound:st057648|FD-Score:4.10|P-value:1.31E-5 ID:SGTC_1899|Compound:st063254|FD-Score:3.39|P-value:2.55E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-3.16|P-value:6.42E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.99|P-value:2.15E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.08|P-value:7.83E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.44|P-value:2.04E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.80|P-value:4.73E-5 ID:SGTC_2625|Compound:euphol|FD-Score:5.58|P-value:5.37E-9 ID:SGTC_2645|Compound:abamectin|FD-Score:3.92|P-value:2.90E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.78|P-value:5.31E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.05|P-value:9.20E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.82|P-value:4.37E-5 ID:SGTC_2787|Compound:5284540|FD-Score:4.01|P-value:1.97E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.54|P-value:1.40E-4 ID:SGTC_2941|Compound:9056145|FD-Score:6.50|P-value:1.33E-11 ID:SGTC_2944|Compound:9067346|FD-Score:-3.82|P-value:4.89E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.91|P-value:2.64E-7 ID:SGTC_2951|Compound:9072950|FD-Score:-9.29|P-value:1.04E-21 ID:SGTC_2987|Compound:9018338|FD-Score:-3.80|P-value:5.26E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.30|P-value:3.86E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.07|P-value:1.48E-5 ID:SGTC_3075|Compound:9116269|FD-Score:3.85|P-value:3.95E-5 ID:SGTC_3142|Compound:9086498|FD-Score:7.17|P-value:9.84E-14 ID:SGTC_3200|Compound:9114385|FD-Score:4.86|P-value:3.06E-7 ID:SGTC_3245|Compound:9134821|FD-Score:-3.04|P-value:9.54E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.33|P-value:3.10E-4 ID:SGTC_3305|Compound:9127027|FD-Score:4.28|P-value:5.52E-6 ID:SGTC_3308|Compound:9128332|FD-Score:5.78|P-value:1.54E-9 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.62|P-value:1.09E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.79|P-value:4.99E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.17|P-value:6.18E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:4.65|P-value:9.41E-7 ID:SGTC_393|Compound:acivicin|FD-Score:3.28|P-value:3.83E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-3.54|P-value:1.51E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.99|P-value:2.30E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-5.60|P-value:5.33E-9 ID:SGTC_628|Compound:1636-0253|FD-Score:3.15|P-value:6.05E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:4.89|P-value:2.71E-7 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.04|P-value:9.63E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.44|P-value:2.27E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.42|P-value:3.23E-6 ID:SGTC_1222|Compound:0366-0074|FD-Score:4.16|P-value:9.87E-6 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.04|P-value:9.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:-3.22|P-value:5.13E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.56|P-value:1.30E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:4.03|P-value:1.76E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.78|P-value:5.79E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.79|P-value:4.95E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.33|P-value:3.11E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.33|P-value:3.16E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.19|P-value:5.85E-8 ID:SGTC_1887|Compound:st057648|FD-Score:4.10|P-value:1.31E-5 ID:SGTC_1899|Compound:st063254|FD-Score:3.39|P-value:2.55E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-3.16|P-value:6.42E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.99|P-value:2.15E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.08|P-value:7.83E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.44|P-value:2.04E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.80|P-value:4.73E-5 ID:SGTC_2625|Compound:euphol|FD-Score:5.58|P-value:5.37E-9 ID:SGTC_2645|Compound:abamectin|FD-Score:3.92|P-value:2.90E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.78|P-value:5.31E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.05|P-value:9.20E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.82|P-value:4.37E-5 ID:SGTC_2787|Compound:5284540|FD-Score:4.01|P-value:1.97E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.54|P-value:1.40E-4 ID:SGTC_2941|Compound:9056145|FD-Score:6.50|P-value:1.33E-11 ID:SGTC_2944|Compound:9067346|FD-Score:-3.82|P-value:4.89E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.91|P-value:2.64E-7 ID:SGTC_2951|Compound:9072950|FD-Score:-9.29|P-value:1.04E-21 ID:SGTC_2987|Compound:9018338|FD-Score:-3.80|P-value:5.26E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.30|P-value:3.86E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.07|P-value:1.48E-5 ID:SGTC_3075|Compound:9116269|FD-Score:3.85|P-value:3.95E-5 ID:SGTC_3142|Compound:9086498|FD-Score:7.17|P-value:9.84E-14 ID:SGTC_3200|Compound:9114385|FD-Score:4.86|P-value:3.06E-7 ID:SGTC_3245|Compound:9134821|FD-Score:-3.04|P-value:9.54E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.33|P-value:3.10E-4 ID:SGTC_3305|Compound:9127027|FD-Score:4.28|P-value:5.52E-6 ID:SGTC_3308|Compound:9128332|FD-Score:5.78|P-value:1.54E-9

Top fitness defect scores for YPL218W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3142 9086498 7.17 49.5 µM 9.84E-14 iron homeostasis 18 3.69E-13 0.21
2 SGTC_2941 9056145 6.50 11.2 µM 1.33E-11 1 3.98E-11 1.29
3 SGTC_3308 9128332 5.78 17.6 µM 1.54E-9 plasma membrane duress 3 3.72E-9 0.63
4 SGTC_2625 euphol 5.58 100.0 µM 5.37E-9 2 1.22E-8 0.21
5 SGTC_806 3966-4455 4.89 5.9 µM 2.71E-7 3 5.15E-7 0.22
6 SGTC_3200 9114385 4.86 49.5 µM 3.06E-7 6 5.79E-7 0.13
7 SGTC_192 3455-0609 4.65 60.9 µM 9.41E-7 5 1.69E-6 0.79
8 SGTC_3305 9127027 4.28 53.8 µM 5.52E-6 2 9.15E-6 0.18
9 SGTC_1222 0366-0074 4.16 1.2 µM 9.87E-6 TSC3-RPN4 2 1.59E-5 0.04
10 SGTC_1887 st057648 4.10 70.8 µM 1.31E-5 4 2.08E-5 0.13
11 SGTC_3068 9116768 4.07 49.5 µM 1.48E-5 6 2.35E-5 0.18
12 SGTC_1383 3049-0032 4.03 20.7 µM 1.76E-5 60S ribosome export 9 2.77E-5 0.26
13 SGTC_2787 5284540 4.01 71.4 µM 1.97E-5 8 3.09E-5 0.08
14 SGTC_2241 6772625 3.99 37.8 µM 2.15E-5 8 3.35E-5 0.02
15 SGTC_2645 abamectin 3.92 100.0 µM 2.90E-5 heme requiring 11 4.46E-5 0.10
16 SGTC_3075 9116269 3.85 49.5 µM 3.95E-5 8 5.99E-5 0.15
17 SGTC_2766 oltipraz 3.82 83.9 µM 4.37E-5 heme requiring 24 6.60E-5 0.01
18 SGTC_2361 9034121 3.80 116.0 µM 4.73E-5 RSC complex & mRNA processing 24 7.12E-5 0.37
19 SGTC_1568 digitonin 3.79 820.0 nM 4.95E-5 sphingolipid biosynthesis & PDR1 17 7.42E-5 0.07
20 SGTC_157 1348-1695 3.79 65.8 µM 4.99E-5 iron homeostasis 37 7.49E-5 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 9.49E-24 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.140 3.52E-16 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.127 1.51E-13 YIL020C HIS6 hom Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
0.111 1.18E-10 YNL244C SUI1 het Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
0.107 4.43E-10 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.103 2.62E-9 YKL187C FAT3 hom Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
0.102 2.69E-9 YLL026W HSP104 hom Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.098 1.23E-8 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.093 5.84E-8 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.092 9.19E-8 YOR052C YOR052C hom Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
0.091 1.38E-7 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.091 1.43E-7 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.090 1.96E-7 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.088 3.46E-7 YDR010C_d YDR010C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.088 3.36E-7 YPL122C TFB2 het Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH