YPL225W

Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL225W / YPL225W

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:3.42|P-value:8.64E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.22|P-value:1.17E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:4.81|P-value:5.44E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.13|P-value:9.48E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:4.31|P-value:3.99E-5 ID:SGTC_819|Compound:1013-0258|FD-Score:5.90|P-value:3.45E-8 ID:SGTC_913|Compound:3487-1245|FD-Score:4.55|P-value:1.59E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-4.15|P-value:9.05E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.52|P-value:6.30E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.77|P-value:2.71E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.68|P-value:3.71E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.72|P-value:3.97E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.48|P-value:7.10E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.52|P-value:7.43E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.46|P-value:7.62E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.97|P-value:1.67E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-4.02|P-value:1.44E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.10|P-value:8.56E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.72|P-value:3.95E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.06|P-value:1.25E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.53|P-value:6.05E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.83|P-value:2.26E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.68|P-value:4.56E-4 ID:SGTC_1743|Compound:st037606|FD-Score:-3.46|P-value:9.11E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.54|P-value:5.84E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-4.67|P-value:1.20E-5 ID:SGTC_1762|Compound:st045503|FD-Score:5.53|P-value:2.16E-7 ID:SGTC_1862|Compound:st057513|FD-Score:4.52|P-value:1.79E-5 ID:SGTC_1870|Compound:st057514|FD-Score:5.90|P-value:3.43E-8 ID:SGTC_1874|Compound:staurosporine|FD-Score:-3.95|P-value:1.83E-4 ID:SGTC_1882|Compound:5615643|FD-Score:-5.09|P-value:2.10E-6 ID:SGTC_2004|Compound:4023728|FD-Score:4.01|P-value:1.21E-4 ID:SGTC_2079|Compound:5212404|FD-Score:-4.24|P-value:6.51E-5 ID:SGTC_2110|Compound:5483698|FD-Score:-3.86|P-value:2.51E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.85|P-value:2.54E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-4.10|P-value:1.07E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.49|P-value:6.77E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.47|P-value:8.83E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.64|P-value:4.28E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.97|P-value:2.75E-6 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.37|P-value:9.86E-4 ID:SGTC_2950|Compound:9072895|FD-Score:4.00|P-value:1.23E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.50|P-value:7.93E-4 ID:SGTC_3173|Compound:9105693|FD-Score:3.71|P-value:3.35E-4 ID:SGTC_3231|Compound:9132313|FD-Score:3.78|P-value:2.70E-4 ID:SGTC_3270|Compound:9137563|FD-Score:3.74|P-value:3.06E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:3.42|P-value:8.64E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.22|P-value:1.17E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:4.81|P-value:5.44E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.13|P-value:9.48E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:4.31|P-value:3.99E-5 ID:SGTC_819|Compound:1013-0258|FD-Score:5.90|P-value:3.45E-8 ID:SGTC_913|Compound:3487-1245|FD-Score:4.55|P-value:1.59E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-4.15|P-value:9.05E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.52|P-value:6.30E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.77|P-value:2.71E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.68|P-value:3.71E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.72|P-value:3.97E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.48|P-value:7.10E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.52|P-value:7.43E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.46|P-value:7.62E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.97|P-value:1.67E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-4.02|P-value:1.44E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.10|P-value:8.56E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.72|P-value:3.95E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.06|P-value:1.25E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.53|P-value:6.05E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.83|P-value:2.26E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.68|P-value:4.56E-4 ID:SGTC_1743|Compound:st037606|FD-Score:-3.46|P-value:9.11E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.54|P-value:5.84E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-4.67|P-value:1.20E-5 ID:SGTC_1762|Compound:st045503|FD-Score:5.53|P-value:2.16E-7 ID:SGTC_1862|Compound:st057513|FD-Score:4.52|P-value:1.79E-5 ID:SGTC_1870|Compound:st057514|FD-Score:5.90|P-value:3.43E-8 ID:SGTC_1874|Compound:staurosporine|FD-Score:-3.95|P-value:1.83E-4 ID:SGTC_1882|Compound:5615643|FD-Score:-5.09|P-value:2.10E-6 ID:SGTC_2004|Compound:4023728|FD-Score:4.01|P-value:1.21E-4 ID:SGTC_2079|Compound:5212404|FD-Score:-4.24|P-value:6.51E-5 ID:SGTC_2110|Compound:5483698|FD-Score:-3.86|P-value:2.51E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.85|P-value:2.54E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-4.10|P-value:1.07E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.49|P-value:6.77E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.47|P-value:8.83E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.64|P-value:4.28E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.97|P-value:2.75E-6 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.37|P-value:9.86E-4 ID:SGTC_2950|Compound:9072895|FD-Score:4.00|P-value:1.23E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.50|P-value:7.93E-4 ID:SGTC_3173|Compound:9105693|FD-Score:3.71|P-value:3.35E-4 ID:SGTC_3231|Compound:9132313|FD-Score:3.78|P-value:2.70E-4 ID:SGTC_3270|Compound:9137563|FD-Score:3.74|P-value:3.06E-4

Top fitness defect scores for YPL225W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1870 st057514 5.90 26.5 µM 3.43E-8 RSC & ERG11 6 1.78E-9
2 SGTC_819 1013-0258 5.90 27.7 µM 3.45E-8 18 1.80E-9
3 SGTC_1762 st045503 5.53 25.5 µM 2.16E-7 8 1.61E-8
4 SGTC_2498 4'-Methoxychalcone 4.97 54.6 µM 2.75E-6 RPP1 & pyrimidine depletion 13 3.37E-7
5 SGTC_346 4513-0042 4.81 7.2 µM 5.44E-6 azole & statin 20 7.61E-7
6 SGTC_913 3487-1245 4.55 167.0 µM 1.59E-5 13 2.74E-6
7 SGTC_1862 st057513 4.52 3.8 µM 1.79E-5 4 3.15E-6
8 SGTC_540 1187-0821 4.31 17.3 µM 3.99E-5 9 8.19E-6
9 SGTC_1596 piperlongumine 4.10 63.0 µM 8.56E-5 15 2.03E-5
10 SGTC_2004 4023728 4.01 41.9 µM 1.21E-4 28 3.07E-5
11 SGTC_2950 9072895 4.00 21.5 µM 1.23E-4 35 3.12E-5
12 SGTC_1682 st012968 3.83 530.0 nM 2.26E-4 20 6.46E-5
13 SGTC_3231 9132313 3.78 49.5 µM 2.70E-4 9 7.96E-5
14 SGTC_1132 3456-3614 3.77 2.5 µM 2.71E-4 tubulin folding & SWR complex 60 8.02E-5
15 SGTC_3270 9137563 3.74 49.5 µM 3.06E-4 RPP1 & pyrimidine depletion 10 9.26E-5
16 SGTC_3173 9105693 3.71 49.5 µM 3.35E-4 29 1.03E-4
17 SGTC_1229 0139-0193 3.68 12.2 µM 3.71E-4 RSC complex & mRNA processing 17 1.16E-4
18 SGTC_2340 7987427 3.64 183.4 µM 4.28E-4 50 1.38E-4
19 SGTC_1746 st038117 3.54 42.0 µM 5.84E-4 46 1.99E-4
20 SGTC_1644 st010134 3.53 20.9 µM 6.05E-4 mitochondrial processes 20 2.07E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.090 1.82E-7 YOR230W WTM1 hom Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
0.090 1.92E-7 YHR040W BCD1 het Essential protein required for the accumulation of box C/D snoRNA
0.087 4.55E-7 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.080 3.92E-6 YNL311C SKP2 hom F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
0.079 4.58E-6 YNL143C_p YNL143C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.079 5.26E-6 YGR104C SRB5 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
0.078 6.00E-6 YMR182C RGM1 hom Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
0.077 8.95E-6 YDR221W GTB1 hom Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
0.076 9.51E-6 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.072 3.25E-5 YIL076W SEC28 hom Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
0.070 4.91E-5 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.068 7.85E-5 YJL082W IML2 hom Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
0.068 8.92E-5 YDR034C LYS14 hom Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
0.066 1.37E-4 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.063 2.55E-4 YOR380W RDR1 hom Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins