YPL227C / ALG5

UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL227C / ALG5

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-7.59|P-value:1.60E-13 ID:SGTC_146|Compound:3448-8650|FD-Score:3.56|P-value:3.17E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.58|P-value:5.56E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.88|P-value:9.64E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.95|P-value:1.02E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:4.12|P-value:3.73E-5 ID:SGTC_397|Compound:arbidol|FD-Score:3.34|P-value:6.74E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:4.03|P-value:5.36E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.35|P-value:6.41E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.52|P-value:3.55E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.27|P-value:2.02E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:6.70|P-value:6.39E-11 ID:SGTC_724|Compound:3910-0327|FD-Score:4.82|P-value:1.81E-6 ID:SGTC_770|Compound:0389-1121|FD-Score:5.29|P-value:1.84E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:3.66|P-value:2.20E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:4.33|P-value:1.62E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:4.37|P-value:1.37E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.34|P-value:6.59E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.13|P-value:3.64E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.96|P-value:9.42E-7 ID:SGTC_989|Compound:1501-0895|FD-Score:3.38|P-value:5.82E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.18|P-value:2.98E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.56|P-value:3.18E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.83|P-value:1.19E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.24|P-value:9.17E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.33|P-value:6.89E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.48|P-value:4.10E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.70|P-value:1.93E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.40|P-value:5.44E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.39|P-value:5.56E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.56|P-value:5.99E-6 ID:SGTC_1512|Compound:0259-0544|FD-Score:5.60|P-value:3.71E-8 ID:SGTC_1613|Compound:st002381|FD-Score:7.02|P-value:8.03E-12 ID:SGTC_1620|Compound:st003704|FD-Score:4.86|P-value:1.55E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.72|P-value:1.76E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.56|P-value:3.18E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.40|P-value:5.48E-4 ID:SGTC_2202|Compound:7226349|FD-Score:3.71|P-value:1.81E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.90|P-value:8.95E-5 ID:SGTC_2254|Compound:7938975|FD-Score:4.46|P-value:9.17E-6 ID:SGTC_2289|Compound:7946404|FD-Score:9.46|P-value:5.23E-20 ID:SGTC_2413|Compound:inermin|FD-Score:4.19|P-value:2.89E-5 ID:SGTC_2432|Compound:5630864|FD-Score:3.91|P-value:8.51E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.70|P-value:1.96E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.48|P-value:4.19E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.41|P-value:5.28E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.73|P-value:1.69E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.66|P-value:2.19E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.31|P-value:7.47E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:5.20|P-value:3.00E-7 ID:SGTC_2643|Compound:anethole|FD-Score:3.48|P-value:4.21E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.86|P-value:1.50E-6 ID:SGTC_2800|Compound:7887935|FD-Score:3.40|P-value:5.38E-4 ID:SGTC_2974|Compound:9094440|FD-Score:4.78|P-value:2.22E-6 ID:SGTC_3037|Compound:9091026|FD-Score:4.83|P-value:1.75E-6 ID:SGTC_3065|Compound:9113888|FD-Score:4.36|P-value:1.43E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.52|P-value:3.67E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.70|P-value:1.93E-4 ID:SGTC_3213|Compound:9129884|FD-Score:4.14|P-value:3.46E-5 ID:SGTC_3232|Compound:9132874|FD-Score:-3.30|P-value:7.83E-4 ID:SGTC_3260|Compound:9137109|FD-Score:7.00|P-value:9.22E-12 ID:SGTC_3265|Compound:9137266|FD-Score:-5.60|P-value:3.92E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-8.58|P-value:8.94E-17 ID:SGTC_3269|Compound:9137823|FD-Score:-3.68|P-value:2.10E-4 ID:SGTC_3275|Compound:9138069|FD-Score:4.12|P-value:3.79E-5 ID:SGTC_3279|Compound:9140807|FD-Score:3.78|P-value:1.40E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.72|P-value:1.78E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.59|P-value:1.60E-13 ID:SGTC_146|Compound:3448-8650|FD-Score:3.56|P-value:3.17E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.58|P-value:5.56E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.88|P-value:9.64E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.95|P-value:1.02E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:4.12|P-value:3.73E-5 ID:SGTC_397|Compound:arbidol|FD-Score:3.34|P-value:6.74E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:4.03|P-value:5.36E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.35|P-value:6.41E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.52|P-value:3.55E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.27|P-value:2.02E-5 ID:SGTC_704|Compound:1310-0270|FD-Score:6.70|P-value:6.39E-11 ID:SGTC_724|Compound:3910-0327|FD-Score:4.82|P-value:1.81E-6 ID:SGTC_770|Compound:0389-1121|FD-Score:5.29|P-value:1.84E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:3.66|P-value:2.20E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:4.33|P-value:1.62E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:4.37|P-value:1.37E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.34|P-value:6.59E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.13|P-value:3.64E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.96|P-value:9.42E-7 ID:SGTC_989|Compound:1501-0895|FD-Score:3.38|P-value:5.82E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.18|P-value:2.98E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.56|P-value:3.18E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.83|P-value:1.19E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.24|P-value:9.17E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.33|P-value:6.89E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.48|P-value:4.10E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.70|P-value:1.93E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.40|P-value:5.44E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.39|P-value:5.56E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.56|P-value:5.99E-6 ID:SGTC_1512|Compound:0259-0544|FD-Score:5.60|P-value:3.71E-8 ID:SGTC_1613|Compound:st002381|FD-Score:7.02|P-value:8.03E-12 ID:SGTC_1620|Compound:st003704|FD-Score:4.86|P-value:1.55E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.72|P-value:1.76E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.56|P-value:3.18E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.40|P-value:5.48E-4 ID:SGTC_2202|Compound:7226349|FD-Score:3.71|P-value:1.81E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.90|P-value:8.95E-5 ID:SGTC_2254|Compound:7938975|FD-Score:4.46|P-value:9.17E-6 ID:SGTC_2289|Compound:7946404|FD-Score:9.46|P-value:5.23E-20 ID:SGTC_2413|Compound:inermin|FD-Score:4.19|P-value:2.89E-5 ID:SGTC_2432|Compound:5630864|FD-Score:3.91|P-value:8.51E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.70|P-value:1.96E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.48|P-value:4.19E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.41|P-value:5.28E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.73|P-value:1.69E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.66|P-value:2.19E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.31|P-value:7.47E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:5.20|P-value:3.00E-7 ID:SGTC_2643|Compound:anethole|FD-Score:3.48|P-value:4.21E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.86|P-value:1.50E-6 ID:SGTC_2800|Compound:7887935|FD-Score:3.40|P-value:5.38E-4 ID:SGTC_2974|Compound:9094440|FD-Score:4.78|P-value:2.22E-6 ID:SGTC_3037|Compound:9091026|FD-Score:4.83|P-value:1.75E-6 ID:SGTC_3065|Compound:9113888|FD-Score:4.36|P-value:1.43E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.52|P-value:3.67E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.70|P-value:1.93E-4 ID:SGTC_3213|Compound:9129884|FD-Score:4.14|P-value:3.46E-5 ID:SGTC_3232|Compound:9132874|FD-Score:-3.30|P-value:7.83E-4 ID:SGTC_3260|Compound:9137109|FD-Score:7.00|P-value:9.22E-12 ID:SGTC_3265|Compound:9137266|FD-Score:-5.60|P-value:3.92E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-8.58|P-value:8.94E-17 ID:SGTC_3269|Compound:9137823|FD-Score:-3.68|P-value:2.10E-4 ID:SGTC_3275|Compound:9138069|FD-Score:4.12|P-value:3.79E-5 ID:SGTC_3279|Compound:9140807|FD-Score:3.78|P-value:1.40E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.72|P-value:1.78E-4

Top fitness defect scores for YPL227C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2289 7946404 9.46 165.1 µM 5.23E-20 9 1.51E-21
2 SGTC_1613 st002381 7.02 52.1 µM 8.03E-12 2 1.13E-12
3 SGTC_3260 9137109 7.00 49.5 µM 9.22E-12 60S ribosome export 1 1.31E-12
4 SGTC_704 1310-0270 6.70 142.0 µM 6.39E-11 4 1.07E-11
5 SGTC_1512 0259-0544 5.60 12.2 µM 3.71E-8 2 1.05E-8
6 SGTC_770 0389-1121 5.29 67.2 µM 1.84E-7 RPP1 & pyrimidine depletion 6 5.96E-8
7 SGTC_2627 acetyl isogambogic acid 5.20 100.0 µM 3.00E-7 TSC3-RPN4 25 1.01E-7
8 SGTC_932 2857-0803 4.96 154.0 µM 9.42E-7 14 3.48E-7
9 SGTC_243 5hr heat shock (37°C) + splitomicin 4.95 1.0 µM 1.02E-6 heat shock/prefoldin 24 3.79E-7
10 SGTC_2688 kanamycin b 4.86 96.7 µM 1.50E-6 calcium & mitochondrial duress 257 5.77E-7
11 SGTC_1620 st003704 4.86 16.5 µM 1.55E-6 6 5.97E-7
12 SGTC_3037 9091026 4.83 49.5 µM 1.75E-6 NEO1-PIK1 81 6.82E-7
13 SGTC_724 3910-0327 4.82 137.0 µM 1.81E-6 ERG2 10 7.04E-7
14 SGTC_2974 9094440 4.78 9.7 µM 2.22E-6 ergosterol biosynthesis 29 8.82E-7
15 SGTC_168 3970-0862 4.58 530.5 µM 5.56E-6 87 2.38E-6
16 SGTC_1505 k297-0037 4.56 20.6 µM 5.99E-6 10 2.57E-6
17 SGTC_2254 7938975 4.46 95.2 µM 9.17E-6 39 4.08E-6
18 SGTC_860 0443-0016 4.37 25.1 µM 1.37E-5 23 6.29E-6
19 SGTC_3065 9113888 4.36 49.5 µM 1.43E-5 16 6.58E-6
20 SGTC_853 0419-0306 4.33 21.0 µM 1.62E-5 9 7.54E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.21E-20 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.148 5.66E-18 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.147 1.05E-17 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.138 9.99E-16 YML019W OST6 hom Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.136 2.14E-15 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.135 3.86E-15 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.127 1.44E-13 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.124 4.81E-13 YJR073C OPI3 hom Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
0.120 2.45E-12 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.111 1.33E-10 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.111 1.39E-10 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.109 2.24E-10 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.106 6.67E-10 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.105 9.31E-10 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.105 1.13E-9 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress