YPL233W / NSL1

Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPL233W / NSL1

Click on Significant Values for Screen Details ID:SGTC_111|Compound:0083-0039|FD-Score:5.03|P-value:4.87E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:3.25|P-value:7.51E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.55|P-value:2.68E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-3.50|P-value:3.58E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-5.76|P-value:1.25E-8 ID:SGTC_720|Compound:r052-1644|FD-Score:-3.23|P-value:8.97E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.41|P-value:1.01E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:3.32|P-value:6.10E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.71|P-value:1.68E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.56|P-value:2.61E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.29|P-value:6.64E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.21|P-value:9.62E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-3.75|P-value:1.45E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-3.37|P-value:5.60E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.50|P-value:3.63E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.32|P-value:6.61E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.42|P-value:4.78E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.99|P-value:5.02E-5 ID:SGTC_2077|Compound:5212015|FD-Score:3.36|P-value:5.26E-4 ID:SGTC_2174|Compound:5790901|FD-Score:3.38|P-value:4.83E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.30|P-value:6.38E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:7.37|P-value:3.85E-13 ID:SGTC_2217|Compound:6934799|FD-Score:-4.23|P-value:2.16E-5 ID:SGTC_2226|Compound:7298446|FD-Score:4.50|P-value:5.96E-6 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.69|P-value:1.78E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.82|P-value:9.87E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.91|P-value:6.88E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:-4.83|P-value:1.50E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.42|P-value:9.44E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.61|P-value:2.14E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.56|P-value:2.94E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.48|P-value:6.28E-6 ID:SGTC_2696|Compound:st077691|FD-Score:12.00|P-value:1.49E-31 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.39|P-value:9.57E-6 ID:SGTC_2698|Compound:st077697|FD-Score:-3.61|P-value:2.45E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.20|P-value:2.46E-7 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.99|P-value:5.73E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.19|P-value:9.25E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-4.22|P-value:2.28E-5 ID:SGTC_3108|Compound:9121785|FD-Score:-4.41|P-value:9.92E-6 ID:SGTC_3151|Compound:9099821|FD-Score:-3.49|P-value:3.73E-4 ID:SGTC_3187|Compound:9109394|FD-Score:4.34|P-value:1.17E-5 ID:SGTC_3250|Compound:9135583|FD-Score:-3.23|P-value:9.14E-4 ID:SGTC_3289|Compound:9119912|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_3294|Compound:9122953|FD-Score:-3.37|P-value:5.70E-4 ID:SGTC_3305|Compound:9127027|FD-Score:3.88|P-value:7.93E-5 ID:SGTC_111|Compound:0083-0039|FD-Score:5.03|P-value:4.87E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:3.25|P-value:7.51E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.55|P-value:2.68E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-3.50|P-value:3.58E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-5.76|P-value:1.25E-8 ID:SGTC_720|Compound:r052-1644|FD-Score:-3.23|P-value:8.97E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.41|P-value:1.01E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:3.32|P-value:6.10E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.71|P-value:1.68E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.56|P-value:2.61E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.29|P-value:6.64E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.21|P-value:9.62E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-3.75|P-value:1.45E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-3.37|P-value:5.60E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.50|P-value:3.63E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.32|P-value:6.61E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.42|P-value:4.78E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.99|P-value:5.02E-5 ID:SGTC_2077|Compound:5212015|FD-Score:3.36|P-value:5.26E-4 ID:SGTC_2174|Compound:5790901|FD-Score:3.38|P-value:4.83E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.30|P-value:6.38E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:7.37|P-value:3.85E-13 ID:SGTC_2217|Compound:6934799|FD-Score:-4.23|P-value:2.16E-5 ID:SGTC_2226|Compound:7298446|FD-Score:4.50|P-value:5.96E-6 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.69|P-value:1.78E-4 ID:SGTC_2360|Compound:9040895|FD-Score:3.82|P-value:9.87E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.91|P-value:6.88E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:-4.83|P-value:1.50E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.42|P-value:9.44E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.61|P-value:2.14E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.56|P-value:2.94E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.48|P-value:6.28E-6 ID:SGTC_2696|Compound:st077691|FD-Score:12.00|P-value:1.49E-31 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.39|P-value:9.57E-6 ID:SGTC_2698|Compound:st077697|FD-Score:-3.61|P-value:2.45E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.20|P-value:2.46E-7 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.99|P-value:5.73E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.19|P-value:9.25E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-4.22|P-value:2.28E-5 ID:SGTC_3108|Compound:9121785|FD-Score:-4.41|P-value:9.92E-6 ID:SGTC_3151|Compound:9099821|FD-Score:-3.49|P-value:3.73E-4 ID:SGTC_3187|Compound:9109394|FD-Score:4.34|P-value:1.17E-5 ID:SGTC_3250|Compound:9135583|FD-Score:-3.23|P-value:9.14E-4 ID:SGTC_3289|Compound:9119912|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_3294|Compound:9122953|FD-Score:-3.37|P-value:5.70E-4 ID:SGTC_3305|Compound:9127027|FD-Score:3.88|P-value:7.93E-5

Top fitness defect scores for YPL233W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2696 st077691 12.00 74.3 µM 1.49E-31 1 2.74E-33 5.08
2 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 7.37 122.8 µM 3.85E-13 1 8.67E-14 2.31
3 SGTC_111 0083-0039 5.03 52.5 µM 4.87E-7 1 2.46E-7 1.88
4 SGTC_2226 7298446 4.50 200.0 µM 5.96E-6 2 3.46E-6 0.87
5 SGTC_2689 tacrolimus 4.48 100.0 µM 6.28E-6 7 3.66E-6 0.01
6 SGTC_2697 mebhydrolin 4.39 72.4 µM 9.57E-6 NEO1 3 5.71E-6 0.11
7 SGTC_3187 9109394 4.34 49.5 µM 1.17E-5 Golgi 5 7.04E-6 0.28
8 SGTC_2064 5220758 3.99 200.0 µM 5.02E-5 6 3.28E-5 0.10
9 SGTC_2567 aureobasidin a 3.91 60.0 nM 6.88E-5 46 4.57E-5 0.10
10 SGTC_3305 9127027 3.88 53.8 µM 7.93E-5 5 5.31E-5 0.12
11 SGTC_2360 9040895 3.82 200.0 µM 9.87E-5 RPP1 & pyrimidine depletion 10 6.69E-5 0.14
12 SGTC_3289 9119912 3.65 71.4 µM 1.86E-4 6 1.30E-4 0.38
13 SGTC_2593 nobiletin 3.61 86.2 µM 2.14E-4 15 1.51E-4 0.14
14 SGTC_1179 1021-1431 3.56 9.3 µM 2.61E-4 8 1.86E-4 0.10
15 SGTC_540 1187-0821 3.55 17.3 µM 2.68E-4 9 1.91E-4 0.02
16 SGTC_2174 5790901 3.38 200.0 µM 4.83E-4 tubulin folding & SWR complex 14 3.56E-4 0.03
17 SGTC_2077 5212015 3.36 200.0 µM 5.26E-4 11 3.90E-4 0.16
18 SGTC_769 0302-0231 3.32 76.0 µM 6.10E-4 24 4.56E-4 0.01
19 SGTC_2179 6298986 3.30 200.0 µM 6.38E-4 heme requiring 10 4.78E-4 0.24
20 SGTC_1284 0929-0043 3.29 16.0 µM 6.64E-4 9 4.99E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 7.87E-21 YNL207W RIO2 het Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
0.141 2.51E-16 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.128 9.32E-14 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.126 1.99E-13 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.121 2.02E-12 YMR235C RNA1 het GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
0.116 1.33E-11 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.107 4.28E-10 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.106 6.35E-10 YOR339C UBC11 hom Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
0.102 3.63E-9 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.100 6.15E-9 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.098 1.30E-8 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.095 3.90E-8 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.094 5.28E-8 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.091 1.14E-7 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.090 1.93E-7 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme