YPL236C / ENV7

Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL236C / ENV7

Click on Significant Values for Screen Details ID:SGTC_24|Compound:0417-1663|FD-Score:3.16|P-value:2.72E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:5.15|P-value:1.00E-8 ID:SGTC_184|Compound:1494-0393|FD-Score:2.89|P-value:7.86E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:4.39|P-value:8.22E-7 ID:SGTC_425|Compound:3825-7017|FD-Score:4.46|P-value:5.69E-7 ID:SGTC_450|Compound:aminopyrene|FD-Score:7.04|P-value:9.03E-15 ID:SGTC_458|Compound:nppb|FD-Score:5.29|P-value:3.91E-9 ID:SGTC_537|Compound:1334-0398|FD-Score:5.01|P-value:2.36E-8 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.43|P-value:1.12E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:2.89|P-value:7.79E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.02|P-value:4.71E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.16|P-value:2.69E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.05|P-value:4.30E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.83|P-value:1.43E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.28|P-value:1.64E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.86|P-value:8.63E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.21|P-value:2.22E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:4.94|P-value:3.49E-8 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.38|P-value:1.10E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.98|P-value:6.93E-6 ID:SGTC_1626|Compound:st003712|FD-Score:-3.89|P-value:1.38E-5 ID:SGTC_1627|Compound:st003713|FD-Score:4.05|P-value:4.96E-6 ID:SGTC_1629|Compound:st005085|FD-Score:5.12|P-value:1.17E-8 ID:SGTC_1636|Compound:st007707|FD-Score:7.44|P-value:2.83E-16 ID:SGTC_1729|Compound:st035615|FD-Score:3.32|P-value:1.40E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-4.12|P-value:4.50E-6 ID:SGTC_1762|Compound:st045503|FD-Score:-4.03|P-value:6.95E-6 ID:SGTC_1766|Compound:st045414|FD-Score:5.44|P-value:1.55E-9 ID:SGTC_1767|Compound:st044992|FD-Score:3.25|P-value:1.89E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.07|P-value:3.93E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-4.69|P-value:2.08E-7 ID:SGTC_1942|Compound:st076330|FD-Score:2.87|P-value:8.25E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.13|P-value:3.05E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.62|P-value:3.88E-5 ID:SGTC_2276|Compound:7947513|FD-Score:3.29|P-value:1.63E-4 ID:SGTC_2372|Compound:9071633|FD-Score:3.32|P-value:1.42E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-2.89|P-value:9.34E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-4.13|P-value:4.33E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.07|P-value:3.89E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:4.98|P-value:2.85E-8 ID:SGTC_2704|Compound:st078022|FD-Score:3.01|P-value:4.88E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.01|P-value:4.87E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.15|P-value:2.88E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.52|P-value:6.07E-5 ID:SGTC_2995|Compound:9067767|FD-Score:2.88|P-value:7.92E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.46|P-value:7.77E-5 ID:SGTC_3003|Compound:9075612|FD-Score:4.12|P-value:3.39E-6 ID:SGTC_3052|Compound:9092717|FD-Score:3.53|P-value:5.65E-5 ID:SGTC_3068|Compound:9116768|FD-Score:3.03|P-value:4.63E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.95|P-value:3.39E-8 ID:SGTC_3205|Compound:9113483|FD-Score:3.53|P-value:5.73E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.60|P-value:2.57E-7 ID:SGTC_3308|Compound:9128332|FD-Score:2.87|P-value:8.48E-4 ID:SGTC_24|Compound:0417-1663|FD-Score:3.16|P-value:2.72E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:5.15|P-value:1.00E-8 ID:SGTC_184|Compound:1494-0393|FD-Score:2.89|P-value:7.86E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:4.39|P-value:8.22E-7 ID:SGTC_425|Compound:3825-7017|FD-Score:4.46|P-value:5.69E-7 ID:SGTC_450|Compound:aminopyrene|FD-Score:7.04|P-value:9.03E-15 ID:SGTC_458|Compound:nppb|FD-Score:5.29|P-value:3.91E-9 ID:SGTC_537|Compound:1334-0398|FD-Score:5.01|P-value:2.36E-8 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.43|P-value:1.12E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:2.89|P-value:7.79E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.02|P-value:4.71E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.16|P-value:2.69E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.05|P-value:4.30E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.83|P-value:1.43E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.28|P-value:1.64E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.86|P-value:8.63E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.21|P-value:2.22E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:4.94|P-value:3.49E-8 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.38|P-value:1.10E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.98|P-value:6.93E-6 ID:SGTC_1626|Compound:st003712|FD-Score:-3.89|P-value:1.38E-5 ID:SGTC_1627|Compound:st003713|FD-Score:4.05|P-value:4.96E-6 ID:SGTC_1629|Compound:st005085|FD-Score:5.12|P-value:1.17E-8 ID:SGTC_1636|Compound:st007707|FD-Score:7.44|P-value:2.83E-16 ID:SGTC_1729|Compound:st035615|FD-Score:3.32|P-value:1.40E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-4.12|P-value:4.50E-6 ID:SGTC_1762|Compound:st045503|FD-Score:-4.03|P-value:6.95E-6 ID:SGTC_1766|Compound:st045414|FD-Score:5.44|P-value:1.55E-9 ID:SGTC_1767|Compound:st044992|FD-Score:3.25|P-value:1.89E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.07|P-value:3.93E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-4.69|P-value:2.08E-7 ID:SGTC_1942|Compound:st076330|FD-Score:2.87|P-value:8.25E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.13|P-value:3.05E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.62|P-value:3.88E-5 ID:SGTC_2276|Compound:7947513|FD-Score:3.29|P-value:1.63E-4 ID:SGTC_2372|Compound:9071633|FD-Score:3.32|P-value:1.42E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-2.89|P-value:9.34E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-4.13|P-value:4.33E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.07|P-value:3.89E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:4.98|P-value:2.85E-8 ID:SGTC_2704|Compound:st078022|FD-Score:3.01|P-value:4.88E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.01|P-value:4.87E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.15|P-value:2.88E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.52|P-value:6.07E-5 ID:SGTC_2995|Compound:9067767|FD-Score:2.88|P-value:7.92E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.46|P-value:7.77E-5 ID:SGTC_3003|Compound:9075612|FD-Score:4.12|P-value:3.39E-6 ID:SGTC_3052|Compound:9092717|FD-Score:3.53|P-value:5.65E-5 ID:SGTC_3068|Compound:9116768|FD-Score:3.03|P-value:4.63E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.95|P-value:3.39E-8 ID:SGTC_3205|Compound:9113483|FD-Score:3.53|P-value:5.73E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.60|P-value:2.57E-7 ID:SGTC_3308|Compound:9128332|FD-Score:2.87|P-value:8.48E-4

Top fitness defect scores for YPL236C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1636 st007707 7.44 68.9 µM 2.83E-16 12 5.21E-14
2 SGTC_450 aminopyrene 7.04 156.0 nM 9.03E-15 4 9.83E-13
3 SGTC_1766 st045414 5.44 44.8 µM 1.55E-9 RPP1 & pyrimidine depletion 7 2.74E-8
4 SGTC_458 nppb 5.29 133.0 µM 3.91E-9 4 6.00E-8
5 SGTC_183 3914-0051 5.15 30.6 µM 1.00E-8 60S ribosome export 4 1.33E-7
6 SGTC_1629 st005085 5.12 44.9 µM 1.17E-8 RPP1 & pyrimidine depletion 12 1.52E-7
7 SGTC_537 1334-0398 5.01 209.0 µM 2.36E-8 9 2.76E-7
8 SGTC_2684 capsaicin 4.98 100.0 µM 2.85E-8 11 3.24E-7
9 SGTC_3200 9114385 4.95 49.5 µM 3.39E-8 15 3.76E-7
10 SGTC_1241 0364-0022 4.94 50.8 µM 3.49E-8 ERG2 17 3.85E-7
11 SGTC_3209 9113616 4.60 49.5 µM 2.57E-7 12 2.10E-6
12 SGTC_425 3825-7017 4.46 13.1 µM 5.69E-7 14 4.13E-6
13 SGTC_424 splitomicin 4.39 100.0 mM 8.22E-7 13 5.65E-6
14 SGTC_3003 9075612 4.12 71.4 µM 3.39E-6 18 1.88E-5
15 SGTC_1627 st003713 4.05 25.6 µM 4.96E-6 calcium & mitochondrial duress 71 2.60E-5
16 SGTC_1600 coumarin 106 3.98 71.1 µM 6.93E-6 26 3.46E-5
17 SGTC_1059 3448-6867 3.83 171.0 µM 1.43E-5 14 6.42E-5
18 SGTC_2129 girgensonine 3.62 121.0 µM 3.88E-5 heme biosynthesis & mitochondrial translocase 41 1.50E-4
19 SGTC_3052 9092717 3.53 49.5 µM 5.65E-5 49 2.06E-4
20 SGTC_3205 9113483 3.53 49.5 µM 5.73E-5 45 2.09E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 5.42E-21 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.135 3.34E-15 YJL125C GCD14 het Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
0.113 5.48E-11 YLL058W_p YLL058W_p hom Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene
0.105 1.27E-9 YLR092W SUL2 hom High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
0.101 4.40E-9 YNL046W_p YNL046W_p hom Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.099 9.43E-9 YJL034W KAR2 het ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
0.094 4.97E-8 YOR161C PNS1 hom Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
0.094 5.63E-8 YMR002W MIC17 hom Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
0.092 9.84E-8 YDL215C GDH2 hom NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels
0.090 1.99E-7 YKL091C YKL091C hom Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study
0.083 1.48E-6 YER183C FAU1 hom 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
0.081 2.41E-6 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.080 3.86E-6 YMR006C PLB2 hom Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
0.079 4.34E-6 YGR180C RNR4 hom Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.078 6.92E-6 YLR056W ERG3 hom C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase