HIPHOP chemogenomics database

YPL254W / HFI1

Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions

Zygosity: Homozygous strain

fixedexpanded
Profile for YPL254W / HFI1

Click on Significant Values for Screen Details

Top fitness defect scores for YPL254W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1982	st072467	8.10	71.9 µM	1.14E-14		5	2.83E-16
2	SGTC_2741	miltefosine	7.44	3.0 µM	1.18E-12		12	5.11E-14
3	SGTC_3303	9125618	6.82	40.6 µM	6.30E-11		7	4.40E-12
4	SGTC_2752	diclazuril	5.87	33.1 µM	1.63E-8		59	2.22E-9
5	SGTC_1877	wortmannin	5.81	25.0 µM	2.19E-8		49	3.09E-9
6	SGTC_129	0088-0017	5.59	176.6 µM	7.08E-8		34	1.15E-8
7	SGTC_889	0919-1524	5.22	82.7 µM	4.43E-7		3	8.97E-8
8	SGTC_2858	9025781	5.10	9.0 µM	7.97E-7	sphingolipid biosynthesis & PDR1	64	1.73E-7
9	SGTC_1884	5633444	4.85	25.0 µM	2.43E-6	redox potentiating	36	6.02E-7
10	SGTC_1916	st067606	4.38	49.2 µM	1.84E-5		22	5.80E-6
11	SGTC_2376	9072922	4.32	81.3 µM	2.39E-5		17	7.79E-6
12	SGTC_2166	5933061	4.19	31.5 µM	4.03E-5	tubulin folding & SWR complex	32	1.39E-5
13	SGTC_70	0141-0289	4.16	43.3 µM	4.49E-5	mitochondrial stress	157	1.57E-5
14	SGTC_3224	9130643	4.11	49.5 µM	5.42E-5	RPP1 & pyrimidine depletion	27	1.95E-5
15	SGTC_3174	9107204	4.10	49.5 µM	5.80E-5	ERG2	24	2.10E-5
16	SGTC_1927	st060202	4.07	13.8 µM	6.36E-5		14	2.32E-5
17	SGTC_2837	9008476	4.03	45.5 µM	7.40E-5		31	2.75E-5
18	SGTC_2577	2',4'-dihydroxy-4-methoxychalcone	4.00	15.3 µM	8.48E-5		33	3.21E-5
19	SGTC_2921	7974240	3.99	10.7 µM	8.65E-5		53	3.28E-5
20	SGTC_562	3772-2733	3.90	112.0 µM	1.21E-4	ERG2	80	4.79E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.215	2.02E-36	YOL051W	GAL11	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.178	2.43E-25	YDR392W	SPT3	hom	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.151	1.36E-18	YBL079W	NUP170	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.150	2.29E-18	YGL066W	SGF73	hom	SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.150	2.78E-18	YGL025C	PGD1	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.146	2.37E-17	YLR087C	CSF1	hom	Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.137	1.62E-15	YLR372W	SUR4	hom	Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
0.137	1.62E-15	YOR290C	SNF2	hom	Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
0.133	9.88E-15	YML073C	RPL6A	hom	Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.129	5.85E-14	YNR001C	CIT1	hom	Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
0.116	1.41E-11	YLR055C	SPT8	hom	Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.115	2.80E-11	YHR059W	FYV4	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.114	3.29E-11	YJR063W	RPA12	hom	RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.113	5.07E-11	YML122C_d	YML122C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.112	8.82E-11	YHL011C	PRS3	hom	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes