YPL254W / HFI1

Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL254W / HFI1

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:3.30|P-value:9.47E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.16|P-value:4.49E-5 ID:SGTC_104|Compound:0074-0074|FD-Score:3.66|P-value:2.86E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:5.59|P-value:7.08E-8 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.42|P-value:5.77E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.52|P-value:4.63E-4 ID:SGTC_458|Compound:nppb|FD-Score:3.85|P-value:1.47E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.90|P-value:1.21E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:3.30|P-value:9.64E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.79|P-value:1.84E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:5.22|P-value:4.43E-7 ID:SGTC_894|Compound:0960-0238|FD-Score:3.34|P-value:8.39E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.44|P-value:5.40E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.43|P-value:6.33E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.28|P-value:9.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.58|P-value:3.86E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.57|P-value:3.89E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.33|P-value:8.81E-4 ID:SGTC_1714|Compound:st033236|FD-Score:-3.70|P-value:2.20E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.20|P-value:3.36E-5 ID:SGTC_1732|Compound:st034308|FD-Score:3.90|P-value:1.23E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.39|P-value:6.43E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.81|P-value:2.19E-8 ID:SGTC_1884|Compound:5633444|FD-Score:4.85|P-value:2.43E-6 ID:SGTC_1916|Compound:st067606|FD-Score:4.38|P-value:1.84E-5 ID:SGTC_1927|Compound:st060202|FD-Score:4.07|P-value:6.36E-5 ID:SGTC_1982|Compound:st072467|FD-Score:8.10|P-value:1.14E-14 ID:SGTC_2030|Compound:5175110|FD-Score:3.75|P-value:2.10E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.19|P-value:4.03E-5 ID:SGTC_2266|Compound:7929956|FD-Score:3.31|P-value:9.37E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.06|P-value:5.95E-5 ID:SGTC_2333|Compound:9017922|FD-Score:-3.39|P-value:6.33E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.32|P-value:2.39E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.89|P-value:1.28E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.44|P-value:5.49E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.00|P-value:8.48E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.45|P-value:5.88E-4 ID:SGTC_2643|Compound:anethole|FD-Score:-3.51|P-value:4.32E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:7.44|P-value:1.18E-12 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.73|P-value:1.99E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:5.87|P-value:1.63E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.60|P-value:6.32E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.43|P-value:5.69E-4 ID:SGTC_2837|Compound:9008476|FD-Score:4.03|P-value:7.40E-5 ID:SGTC_2858|Compound:9025781|FD-Score:5.10|P-value:7.97E-7 ID:SGTC_2880|Compound:9042983|FD-Score:-3.96|P-value:8.63E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-5.54|P-value:7.63E-8 ID:SGTC_2921|Compound:7974240|FD-Score:3.99|P-value:8.65E-5 ID:SGTC_2923|Compound:7992654|FD-Score:3.38|P-value:7.43E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.36|P-value:7.02E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3143|Compound:9086658|FD-Score:3.54|P-value:4.39E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.63|P-value:2.84E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.10|P-value:5.80E-5 ID:SGTC_3181|Compound:9108277|FD-Score:-3.82|P-value:1.44E-4 ID:SGTC_3224|Compound:9130643|FD-Score:4.11|P-value:5.42E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-3.43|P-value:5.68E-4 ID:SGTC_3303|Compound:9125618|FD-Score:6.82|P-value:6.30E-11 ID:SGTC_3319|Compound:9138450|FD-Score:-5.33|P-value:2.17E-7 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.30|P-value:9.47E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.16|P-value:4.49E-5 ID:SGTC_104|Compound:0074-0074|FD-Score:3.66|P-value:2.86E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:5.59|P-value:7.08E-8 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.42|P-value:5.77E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.52|P-value:4.63E-4 ID:SGTC_458|Compound:nppb|FD-Score:3.85|P-value:1.47E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.90|P-value:1.21E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:3.30|P-value:9.64E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.79|P-value:1.84E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:5.22|P-value:4.43E-7 ID:SGTC_894|Compound:0960-0238|FD-Score:3.34|P-value:8.39E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.44|P-value:5.40E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.43|P-value:6.33E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.28|P-value:9.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.58|P-value:3.86E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.57|P-value:3.89E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.33|P-value:8.81E-4 ID:SGTC_1714|Compound:st033236|FD-Score:-3.70|P-value:2.20E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.20|P-value:3.36E-5 ID:SGTC_1732|Compound:st034308|FD-Score:3.90|P-value:1.23E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.39|P-value:6.43E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.81|P-value:2.19E-8 ID:SGTC_1884|Compound:5633444|FD-Score:4.85|P-value:2.43E-6 ID:SGTC_1916|Compound:st067606|FD-Score:4.38|P-value:1.84E-5 ID:SGTC_1927|Compound:st060202|FD-Score:4.07|P-value:6.36E-5 ID:SGTC_1982|Compound:st072467|FD-Score:8.10|P-value:1.14E-14 ID:SGTC_2030|Compound:5175110|FD-Score:3.75|P-value:2.10E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.19|P-value:4.03E-5 ID:SGTC_2266|Compound:7929956|FD-Score:3.31|P-value:9.37E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.06|P-value:5.95E-5 ID:SGTC_2333|Compound:9017922|FD-Score:-3.39|P-value:6.33E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.32|P-value:2.39E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.89|P-value:1.28E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.44|P-value:5.49E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.00|P-value:8.48E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.45|P-value:5.88E-4 ID:SGTC_2643|Compound:anethole|FD-Score:-3.51|P-value:4.32E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:7.44|P-value:1.18E-12 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.73|P-value:1.99E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:5.87|P-value:1.63E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.60|P-value:6.32E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.43|P-value:5.69E-4 ID:SGTC_2837|Compound:9008476|FD-Score:4.03|P-value:7.40E-5 ID:SGTC_2858|Compound:9025781|FD-Score:5.10|P-value:7.97E-7 ID:SGTC_2880|Compound:9042983|FD-Score:-3.96|P-value:8.63E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-5.54|P-value:7.63E-8 ID:SGTC_2921|Compound:7974240|FD-Score:3.99|P-value:8.65E-5 ID:SGTC_2923|Compound:7992654|FD-Score:3.38|P-value:7.43E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.36|P-value:7.02E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3143|Compound:9086658|FD-Score:3.54|P-value:4.39E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.63|P-value:2.84E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.10|P-value:5.80E-5 ID:SGTC_3181|Compound:9108277|FD-Score:-3.82|P-value:1.44E-4 ID:SGTC_3224|Compound:9130643|FD-Score:4.11|P-value:5.42E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-3.43|P-value:5.68E-4 ID:SGTC_3303|Compound:9125618|FD-Score:6.82|P-value:6.30E-11 ID:SGTC_3319|Compound:9138450|FD-Score:-5.33|P-value:2.17E-7

Top fitness defect scores for YPL254W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1982 st072467 8.10 71.9 µM 1.14E-14 5 2.83E-16
2 SGTC_2741 miltefosine 7.44 3.0 µM 1.18E-12 12 5.11E-14
3 SGTC_3303 9125618 6.82 40.6 µM 6.30E-11 7 4.40E-12
4 SGTC_2752 diclazuril 5.87 33.1 µM 1.63E-8 59 2.22E-9
5 SGTC_1877 wortmannin 5.81 25.0 µM 2.19E-8 49 3.09E-9
6 SGTC_129 0088-0017 5.59 176.6 µM 7.08E-8 34 1.15E-8
7 SGTC_889 0919-1524 5.22 82.7 µM 4.43E-7 3 8.97E-8
8 SGTC_2858 9025781 5.10 9.0 µM 7.97E-7 sphingolipid biosynthesis & PDR1 64 1.73E-7
9 SGTC_1884 5633444 4.85 25.0 µM 2.43E-6 redox potentiating 36 6.02E-7
10 SGTC_1916 st067606 4.38 49.2 µM 1.84E-5 22 5.80E-6
11 SGTC_2376 9072922 4.32 81.3 µM 2.39E-5 17 7.79E-6
12 SGTC_2166 5933061 4.19 31.5 µM 4.03E-5 tubulin folding & SWR complex 32 1.39E-5
13 SGTC_70 0141-0289 4.16 43.3 µM 4.49E-5 mitochondrial stress 157 1.57E-5
14 SGTC_3224 9130643 4.11 49.5 µM 5.42E-5 RPP1 & pyrimidine depletion 27 1.95E-5
15 SGTC_3174 9107204 4.10 49.5 µM 5.80E-5 ERG2 24 2.10E-5
16 SGTC_1927 st060202 4.07 13.8 µM 6.36E-5 14 2.32E-5
17 SGTC_2837 9008476 4.03 45.5 µM 7.40E-5 31 2.75E-5
18 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 4.00 15.3 µM 8.48E-5 33 3.21E-5
19 SGTC_2921 7974240 3.99 10.7 µM 8.65E-5 53 3.28E-5
20 SGTC_562 3772-2733 3.90 112.0 µM 1.21E-4 ERG2 80 4.79E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.215 2.02E-36 YOL051W GAL11 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.178 2.43E-25 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.151 1.36E-18 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.150 2.29E-18 YGL066W SGF73 hom SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.150 2.78E-18 YGL025C PGD1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.146 2.37E-17 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.137 1.62E-15 YLR372W SUR4 hom Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
0.137 1.62E-15 YOR290C SNF2 hom Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
0.133 9.88E-15 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.129 5.85E-14 YNR001C CIT1 hom Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
0.116 1.41E-11 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.115 2.80E-11 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.114 3.29E-11 YJR063W RPA12 hom RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.113 5.07E-11 YML122C_d YML122C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.112 8.82E-11 YHL011C PRS3 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes