YPL259C / APM1

Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL259C / APM1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:5.53|P-value:5.61E-7 ID:SGTC_57|Compound:miconazole|FD-Score:6.13|P-value:3.36E-8 ID:SGTC_61|Compound:0978-8327|FD-Score:3.79|P-value:4.21E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.26|P-value:8.99E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.67|P-value:6.16E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:4.36|P-value:6.27E-5 ID:SGTC_263|Compound:alizarin|FD-Score:7.08|P-value:2.33E-10 ID:SGTC_269|Compound:exalamide|FD-Score:4.61|P-value:2.48E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:10.30|P-value:8.91E-20 ID:SGTC_320|Compound:4092-0846|FD-Score:5.66|P-value:3.07E-7 ID:SGTC_327|Compound:1273-0057|FD-Score:3.65|P-value:6.59E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:4.53|P-value:3.33E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.56|P-value:8.46E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.87|P-value:7.11E-10 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-4.35|P-value:6.26E-5 ID:SGTC_469|Compound:w7|FD-Score:3.63|P-value:6.95E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.74|P-value:4.98E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.73|P-value:5.11E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:7.07|P-value:2.37E-10 ID:SGTC_609|Compound:0986-0026|FD-Score:4.27|P-value:8.47E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:4.09|P-value:1.61E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:5.26|P-value:1.81E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:8.42|P-value:6.24E-14 ID:SGTC_778|Compound:3456-2434|FD-Score:3.52|P-value:9.68E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.05|P-value:1.81E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.02|P-value:1.98E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.56|P-value:8.53E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.15|P-value:1.29E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.03|P-value:1.97E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.87|P-value:3.27E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.46|P-value:4.27E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.02|P-value:4.96E-6 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.97|P-value:2.33E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.83|P-value:3.67E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.66|P-value:2.07E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:-3.71|P-value:5.49E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:4.06|P-value:1.73E-4 ID:SGTC_1604|Compound:st002052|FD-Score:5.82|P-value:1.53E-7 ID:SGTC_1707|Compound:st032323|FD-Score:3.63|P-value:6.99E-4 ID:SGTC_1708|Compound:st033234|FD-Score:5.06|P-value:4.25E-6 ID:SGTC_1828|Compound:st055811|FD-Score:3.98|P-value:2.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.79|P-value:4.25E-4 ID:SGTC_1909|Compound:st060837|FD-Score:4.05|P-value:1.81E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.77|P-value:4.58E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.63|P-value:6.91E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.24|P-value:1.95E-6 ID:SGTC_2319|Compound:6033012|FD-Score:-3.52|P-value:9.69E-4 ID:SGTC_2335|Compound:9018011|FD-Score:4.81|P-value:1.13E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.46|P-value:4.32E-5 ID:SGTC_2912|Compound:7964298|FD-Score:-3.55|P-value:8.76E-4 ID:SGTC_2988|Compound:9073640|FD-Score:4.01|P-value:2.05E-4 ID:SGTC_3068|Compound:9116768|FD-Score:5.42|P-value:9.16E-7 ID:SGTC_3152|Compound:9099253|FD-Score:7.68|P-value:6.94E-12 ID:SGTC_3181|Compound:9108277|FD-Score:4.33|P-value:6.96E-5 ID:SGTC_3293|Compound:9120939|FD-Score:5.19|P-value:2.42E-6 ID:SGTC_55|Compound:0767-0285|FD-Score:5.53|P-value:5.61E-7 ID:SGTC_57|Compound:miconazole|FD-Score:6.13|P-value:3.36E-8 ID:SGTC_61|Compound:0978-8327|FD-Score:3.79|P-value:4.21E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.26|P-value:8.99E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.67|P-value:6.16E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:4.36|P-value:6.27E-5 ID:SGTC_263|Compound:alizarin|FD-Score:7.08|P-value:2.33E-10 ID:SGTC_269|Compound:exalamide|FD-Score:4.61|P-value:2.48E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:10.30|P-value:8.91E-20 ID:SGTC_320|Compound:4092-0846|FD-Score:5.66|P-value:3.07E-7 ID:SGTC_327|Compound:1273-0057|FD-Score:3.65|P-value:6.59E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:4.53|P-value:3.33E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.56|P-value:8.46E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.87|P-value:7.11E-10 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-4.35|P-value:6.26E-5 ID:SGTC_469|Compound:w7|FD-Score:3.63|P-value:6.95E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.74|P-value:4.98E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.73|P-value:5.11E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:7.07|P-value:2.37E-10 ID:SGTC_609|Compound:0986-0026|FD-Score:4.27|P-value:8.47E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:4.09|P-value:1.61E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:5.26|P-value:1.81E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:8.42|P-value:6.24E-14 ID:SGTC_778|Compound:3456-2434|FD-Score:3.52|P-value:9.68E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.05|P-value:1.81E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.02|P-value:1.98E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.56|P-value:8.53E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.15|P-value:1.29E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.03|P-value:1.97E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.87|P-value:3.27E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.46|P-value:4.27E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.02|P-value:4.96E-6 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.97|P-value:2.33E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.83|P-value:3.67E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.66|P-value:2.07E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:-3.71|P-value:5.49E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:4.06|P-value:1.73E-4 ID:SGTC_1604|Compound:st002052|FD-Score:5.82|P-value:1.53E-7 ID:SGTC_1707|Compound:st032323|FD-Score:3.63|P-value:6.99E-4 ID:SGTC_1708|Compound:st033234|FD-Score:5.06|P-value:4.25E-6 ID:SGTC_1828|Compound:st055811|FD-Score:3.98|P-value:2.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.79|P-value:4.25E-4 ID:SGTC_1909|Compound:st060837|FD-Score:4.05|P-value:1.81E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.77|P-value:4.58E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.63|P-value:6.91E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.24|P-value:1.95E-6 ID:SGTC_2319|Compound:6033012|FD-Score:-3.52|P-value:9.69E-4 ID:SGTC_2335|Compound:9018011|FD-Score:4.81|P-value:1.13E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.46|P-value:4.32E-5 ID:SGTC_2912|Compound:7964298|FD-Score:-3.55|P-value:8.76E-4 ID:SGTC_2988|Compound:9073640|FD-Score:4.01|P-value:2.05E-4 ID:SGTC_3068|Compound:9116768|FD-Score:5.42|P-value:9.16E-7 ID:SGTC_3152|Compound:9099253|FD-Score:7.68|P-value:6.94E-12 ID:SGTC_3181|Compound:9108277|FD-Score:4.33|P-value:6.96E-5 ID:SGTC_3293|Compound:9120939|FD-Score:5.19|P-value:2.42E-6

Top fitness defect scores for YPL259C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_312 4092-0653 10.30 6.8 µM 8.91E-20 ergosterol depletion effects on membrane 11 5.85E-25
2 SGTC_768 0384-0033 8.42 77.3 µM 6.24E-14 fatty acid desaturase (OLE1) 20 1.92E-17
3 SGTC_3152 9099253 7.68 49.5 µM 6.94E-12 NEO1-PIK1 37 8.15E-15
4 SGTC_263 alizarin 7.08 107.9 µM 2.33E-10 iron homeostasis 14 7.38E-13
5 SGTC_568 1193-0097 7.07 7.5 µM 2.37E-10 ERAD & cell cycle 47 7.58E-13
6 SGTC_57 miconazole 6.13 162.5 nM 3.36E-8 ergosterol depletion effects on membrane 9 4.34E-10
7 SGTC_1604 st002052 5.82 72.4 µM 1.53E-7 TSC3-RPN4 6 3.02E-9
8 SGTC_320 4092-0846 5.66 5.3 µM 3.07E-7 ergosterol depletion effects on membrane 17 7.39E-9
9 SGTC_55 0767-0285 5.53 6.6 µM 5.61E-7 21 1.59E-8
10 SGTC_3068 9116768 5.42 49.5 µM 9.16E-7 5 2.99E-8
11 SGTC_715 0868-0130 5.26 184.0 µM 1.81E-6 3 7.14E-8
12 SGTC_2249 haloperidol 5.24 116.4 µM 1.95E-6 ergosterol depletion effects on membrane 58 7.86E-8
13 SGTC_3293 9120939 5.19 62.0 µM 2.42E-6 3 1.04E-7
14 SGTC_1708 st033234 5.06 31.8 µM 4.25E-6 6 2.13E-7
15 SGTC_1161 k007-0610 5.02 110.0 µM 4.96E-6 ERAD & cell cycle 80 2.59E-7
16 SGTC_2335 9018011 4.81 154.2 µM 1.13E-5 15 7.44E-7
17 SGTC_1386 3228-0095 4.66 33.8 µM 2.07E-5 11 1.60E-6
18 SGTC_269 exalamide 4.61 29.0 µM 2.48E-5 ergosterol biosynthesis 12 2.02E-6
19 SGTC_419 nsc-638432 4.53 893.0 µM 3.33E-5 DNA damage response 6 2.94E-6
20 SGTC_1157 4092-0394 4.46 27.6 µM 4.27E-5 19 4.04E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 9.44E-49 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.244 1.55E-46 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.220 4.10E-38 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.219 8.17E-38 YLR350W ORM2 hom Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
0.215 2.61E-36 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.210 6.93E-35 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.202 4.16E-32 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.197 1.28E-30 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.193 1.43E-29 YOR070C GYP1 hom Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.188 3.79E-28 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.185 3.47E-27 YER031C YPT31 hom Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.185 3.42E-27 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.184 6.90E-27 YIL002C INP51 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
0.177 5.87E-25 YKL190W CNB1 hom Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
0.176 1.07E-24 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data