YPL269W / KAR9

Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL269W / KAR9

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:6.79|P-value:2.64E-10 ID:SGTC_47|Compound:0149-0135|FD-Score:4.59|P-value:1.55E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.76|P-value:3.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:19.40|P-value:2.77E-72 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.42|P-value:5.09E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.94|P-value:1.86E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:14.40|P-value:9.02E-41 ID:SGTC_304|Compound:1310-0298|FD-Score:7.64|P-value:1.26E-12 ID:SGTC_502|Compound:l-744,832|FD-Score:3.60|P-value:5.88E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.18|P-value:7.66E-5 ID:SGTC_552|Compound:k242-0005|FD-Score:-3.41|P-value:5.37E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.51|P-value:7.83E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.46|P-value:9.04E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.43|P-value:4.87E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:-3.40|P-value:5.53E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:5.23|P-value:9.73E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:3.92|P-value:1.95E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.65|P-value:1.20E-5 ID:SGTC_781|Compound:4335-2806|FD-Score:6.13|P-value:1.07E-8 ID:SGTC_829|Compound:1635-0120|FD-Score:4.03|P-value:1.35E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.85|P-value:5.28E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.63|P-value:5.31E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.21|P-value:1.03E-6 ID:SGTC_971|Compound:1315-0384|FD-Score:3.89|P-value:2.20E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:-3.59|P-value:2.91E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.26|P-value:5.74E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:-4.96|P-value:1.22E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.72|P-value:9.05E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:4.16|P-value:8.40E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.47|P-value:2.49E-5 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.69|P-value:4.36E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:6.95|P-value:9.87E-11 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.45|P-value:2.70E-5 ID:SGTC_1616|Compound:st002734|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_1656|Compound:st012921|FD-Score:3.82|P-value:2.82E-4 ID:SGTC_1659|Compound:st012954|FD-Score:5.62|P-value:1.49E-7 ID:SGTC_1684|Compound:st024538|FD-Score:3.76|P-value:3.39E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.03|P-value:1.34E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.57|P-value:1.71E-5 ID:SGTC_1774|Compound:st048371|FD-Score:3.80|P-value:2.96E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.50|P-value:8.14E-4 ID:SGTC_1842|Compound:st055362|FD-Score:3.86|P-value:2.42E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.46|P-value:9.30E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-4.59|P-value:6.09E-6 ID:SGTC_2047|Compound:4141938|FD-Score:4.50|P-value:2.24E-5 ID:SGTC_2425|Compound:5628681|FD-Score:4.75|P-value:8.00E-6 ID:SGTC_2439|Compound:5492030|FD-Score:-4.50|P-value:9.10E-6 ID:SGTC_2444|Compound:5485484|FD-Score:-3.39|P-value:5.72E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.73|P-value:3.85E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.69|P-value:1.01E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.67|P-value:1.16E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.91|P-value:9.37E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:5.72|P-value:8.97E-8 ID:SGTC_2585|Compound:alliin|FD-Score:5.50|P-value:2.71E-7 ID:SGTC_2969|Compound:9091408|FD-Score:3.84|P-value:2.59E-4 ID:SGTC_2972|Compound:9093619|FD-Score:6.97|P-value:8.60E-11 ID:SGTC_2973|Compound:9094665|FD-Score:-4.74|P-value:3.25E-6 ID:SGTC_3014|Compound:9081533|FD-Score:-3.24|P-value:9.04E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-4.10|P-value:4.54E-5 ID:SGTC_3187|Compound:9109394|FD-Score:3.80|P-value:2.99E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.94|P-value:1.86E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.24|P-value:9.26E-7 ID:SGTC_3311|Compound:9131146|FD-Score:3.47|P-value:8.83E-4 ID:SGTC_3350|Compound:9150499|FD-Score:5.74|P-value:8.19E-8 ID:SGTC_3351|Compound:9150312|FD-Score:3.96|P-value:1.70E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:6.79|P-value:2.64E-10 ID:SGTC_47|Compound:0149-0135|FD-Score:4.59|P-value:1.55E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.76|P-value:3.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:19.40|P-value:2.77E-72 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.42|P-value:5.09E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.94|P-value:1.86E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:14.40|P-value:9.02E-41 ID:SGTC_304|Compound:1310-0298|FD-Score:7.64|P-value:1.26E-12 ID:SGTC_502|Compound:l-744,832|FD-Score:3.60|P-value:5.88E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.18|P-value:7.66E-5 ID:SGTC_552|Compound:k242-0005|FD-Score:-3.41|P-value:5.37E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.51|P-value:7.83E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.46|P-value:9.04E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.43|P-value:4.87E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:-3.40|P-value:5.53E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:5.23|P-value:9.73E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:3.92|P-value:1.95E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.65|P-value:1.20E-5 ID:SGTC_781|Compound:4335-2806|FD-Score:6.13|P-value:1.07E-8 ID:SGTC_829|Compound:1635-0120|FD-Score:4.03|P-value:1.35E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.85|P-value:5.28E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.63|P-value:5.31E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.21|P-value:1.03E-6 ID:SGTC_971|Compound:1315-0384|FD-Score:3.89|P-value:2.20E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:-3.59|P-value:2.91E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.26|P-value:5.74E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:-4.96|P-value:1.22E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.72|P-value:9.05E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:4.16|P-value:8.40E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.47|P-value:2.49E-5 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.69|P-value:4.36E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:6.95|P-value:9.87E-11 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.45|P-value:2.70E-5 ID:SGTC_1616|Compound:st002734|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_1656|Compound:st012921|FD-Score:3.82|P-value:2.82E-4 ID:SGTC_1659|Compound:st012954|FD-Score:5.62|P-value:1.49E-7 ID:SGTC_1684|Compound:st024538|FD-Score:3.76|P-value:3.39E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.03|P-value:1.34E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.57|P-value:1.71E-5 ID:SGTC_1774|Compound:st048371|FD-Score:3.80|P-value:2.96E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.50|P-value:8.14E-4 ID:SGTC_1842|Compound:st055362|FD-Score:3.86|P-value:2.42E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.46|P-value:9.30E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-4.59|P-value:6.09E-6 ID:SGTC_2047|Compound:4141938|FD-Score:4.50|P-value:2.24E-5 ID:SGTC_2425|Compound:5628681|FD-Score:4.75|P-value:8.00E-6 ID:SGTC_2439|Compound:5492030|FD-Score:-4.50|P-value:9.10E-6 ID:SGTC_2444|Compound:5485484|FD-Score:-3.39|P-value:5.72E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.73|P-value:3.85E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.69|P-value:1.01E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.67|P-value:1.16E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.91|P-value:9.37E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:5.72|P-value:8.97E-8 ID:SGTC_2585|Compound:alliin|FD-Score:5.50|P-value:2.71E-7 ID:SGTC_2969|Compound:9091408|FD-Score:3.84|P-value:2.59E-4 ID:SGTC_2972|Compound:9093619|FD-Score:6.97|P-value:8.60E-11 ID:SGTC_2973|Compound:9094665|FD-Score:-4.74|P-value:3.25E-6 ID:SGTC_3014|Compound:9081533|FD-Score:-3.24|P-value:9.04E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-4.10|P-value:4.54E-5 ID:SGTC_3187|Compound:9109394|FD-Score:3.80|P-value:2.99E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.94|P-value:1.86E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.24|P-value:9.26E-7 ID:SGTC_3311|Compound:9131146|FD-Score:3.47|P-value:8.83E-4 ID:SGTC_3350|Compound:9150499|FD-Score:5.74|P-value:8.19E-8 ID:SGTC_3351|Compound:9150312|FD-Score:3.96|P-value:1.70E-4

Top fitness defect scores for YPL269W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 19.40 9.0 µM 2.77E-72 cell wall 12 4.03E-84
2 SGTC_138 0850-0259 14.40 40.9 µM 9.02E-41 cell wall 17 1.73E-47
3 SGTC_304 1310-0298 7.64 97.0 µM 1.26E-12 cell wall 8 1.11E-14
4 SGTC_2972 9093619 6.97 59.9 µM 8.60E-11 1 1.55E-12
5 SGTC_1562 morin hydrate 6.95 59.1 µM 9.87E-11 1 1.82E-12
6 SGTC_43 0570-0105 6.79 72.5 µM 2.64E-10 ERG2 24 5.73E-12
7 SGTC_781 4335-2806 6.13 94.4 µM 1.07E-8 5 4.37E-10
8 SGTC_3350 9150499 5.74 3.6 µM 8.19E-8 cell wall 18 4.75E-9
9 SGTC_2584 androsterone 5.72 48.7 µM 8.97E-8 cell wall 64 5.29E-9
10 SGTC_2538 3',7'-epoxycaryophyllan-6-one 5.67 100.0 µM 1.16E-7 5 7.15E-9
11 SGTC_1659 st012954 5.62 42.1 µM 1.49E-7 4 9.56E-9
12 SGTC_2585 alliin 5.50 100.0 µM 2.71E-7 9 1.93E-8
13 SGTC_3211 9128781 5.24 49.5 µM 9.26E-7 30 8.16E-8
14 SGTC_739 1486-1183 5.23 203.0 µM 9.73E-7 5 8.65E-8
15 SGTC_951 1171-0579 5.21 82.7 µM 1.03E-6 cell wall 41 9.30E-8
16 SGTC_839 0122-0028 4.85 230.0 µM 5.28E-6 RSC complex & mRNA processing 16 6.30E-7
17 SGTC_2425 5628681 4.75 7.8 µM 8.00E-6 6 1.03E-6
18 SGTC_1157 4092-0394 4.72 27.6 µM 9.05E-6 16 1.19E-6
19 SGTC_2510 benzyl isothiocyanate 4.69 8.3 µM 1.01E-5 14 1.35E-6
20 SGTC_773 4358-1457 4.65 44.6 µM 1.20E-5 26 1.65E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.424 2.77E-146 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.399 2.08E-128 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.371 3.93E-110 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.371 4.59E-110 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.353 5.22E-99 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.347 2.77E-95 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.340 1.06E-91 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.337 7.94E-90 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.336 4.76E-89 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.325 2.71E-83 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.324 6.67E-83 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.317 2.47E-79 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.315 3.86E-78 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.314 9.46E-78 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.313 4.08E-77 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene