YPL274W / SAM3

High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL274W / SAM3

Click on Significant Values for Screen Details ID:SGTC_101|Compound:0490-5701|FD-Score:3.61|P-value:2.97E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.05|P-value:9.72E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.15|P-value:3.73E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.41|P-value:2.72E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.03|P-value:2.48E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.15|P-value:7.01E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.45|P-value:2.40E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:6.57|P-value:1.16E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:3.70|P-value:2.16E-4 ID:SGTC_850|Compound:0388-0143|FD-Score:3.50|P-value:4.41E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.12|P-value:7.65E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:3.59|P-value:3.23E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.39|P-value:6.49E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.82|P-value:1.36E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.44|P-value:2.48E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.65|P-value:2.58E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-4.72|P-value:1.06E-6 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.42|P-value:5.96E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:3.79|P-value:1.54E-4 ID:SGTC_1762|Compound:st045503|FD-Score:-3.10|P-value:8.29E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.23|P-value:5.18E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.23|P-value:5.30E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.83|P-value:5.59E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:-5.56|P-value:1.37E-8 ID:SGTC_2607|Compound:solasodine|FD-Score:5.03|P-value:7.14E-7 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.19|P-value:1.24E-5 ID:SGTC_2647|Compound:atorvastatin|FD-Score:-3.08|P-value:8.77E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:-3.27|P-value:4.65E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-4.00|P-value:2.73E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-3.24|P-value:5.07E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-3.95|P-value:3.39E-5 ID:SGTC_3220|Compound:9131269|FD-Score:3.56|P-value:3.62E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:3.61|P-value:2.97E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.05|P-value:9.72E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.15|P-value:3.73E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.41|P-value:2.72E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.03|P-value:2.48E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.15|P-value:7.01E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.45|P-value:2.40E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:6.57|P-value:1.16E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:3.70|P-value:2.16E-4 ID:SGTC_850|Compound:0388-0143|FD-Score:3.50|P-value:4.41E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.12|P-value:7.65E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:3.59|P-value:3.23E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.39|P-value:6.49E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.82|P-value:1.36E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.44|P-value:2.48E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.65|P-value:2.58E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-4.72|P-value:1.06E-6 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.42|P-value:5.96E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:3.79|P-value:1.54E-4 ID:SGTC_1762|Compound:st045503|FD-Score:-3.10|P-value:8.29E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.23|P-value:5.18E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.23|P-value:5.30E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.83|P-value:5.59E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:-5.56|P-value:1.37E-8 ID:SGTC_2607|Compound:solasodine|FD-Score:5.03|P-value:7.14E-7 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.19|P-value:1.24E-5 ID:SGTC_2647|Compound:atorvastatin|FD-Score:-3.08|P-value:8.77E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:-3.27|P-value:4.65E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-4.00|P-value:2.73E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-3.24|P-value:5.07E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-3.95|P-value:3.39E-5 ID:SGTC_3220|Compound:9131269|FD-Score:3.56|P-value:3.62E-4

Top fitness defect scores for YPL274W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_636 0kpi-0074 6.57 126.0 µM 1.16E-10 5 2.45E-11
2 SGTC_2607 solasodine 5.03 47.4 µM 7.14E-7 36 2.49E-7
3 SGTC_160 3453-0671 4.15 289.7 µM 3.73E-5 36 1.66E-5
4 SGTC_1262 0737-0114 3.82 49.6 µM 1.36E-4 22 6.58E-5
5 SGTC_1485 3909-9022 3.79 254.0 µM 1.54E-4 RPP1 & pyrimidine depletion 11 7.53E-5
6 SGTC_829 1635-0120 3.70 117.0 µM 2.16E-4 DNA damage response 49 1.08E-4
7 SGTC_1330 1469-0054 3.65 61.8 µM 2.58E-4 iron homeostasis 24 1.30E-4
8 SGTC_101 0490-5701 3.61 117.5 µM 2.97E-4 20 1.51E-4
9 SGTC_1197 1601-0112 3.59 267.0 µM 3.23E-4 29 1.65E-4
10 SGTC_3220 9131269 3.56 49.5 µM 3.62E-4 19 1.87E-4
11 SGTC_850 0388-0143 3.50 75.9 µM 4.41E-4 23 2.31E-4
12 SGTC_1457 k015-0032 3.42 57.3 µM 5.96E-4 60 3.18E-4
13 SGTC_1223 0764-0573 3.39 147.0 µM 6.49E-4 43 3.48E-4
14 SGTC_1015 4130-4525 3.26 82.0 µM 0.00101 60S ribosome export 46 5.59E-4
15 SGTC_954 1189-0853 3.24 2.9 µM 0.00106 endomembrane recycling 71 5.90E-4
16 SGTC_397 arbidol 3.20 50.0 µM 0.00123 cell wall 50 6.92E-4
17 SGTC_948 3448-0295 3.19 75.3 µM 0.00125 67 7.03E-4
18 SGTC_2679 rapamycin 3.18 1.0 nM 0.00131 209 7.40E-4
19 SGTC_3233 9132340 3.06 49.5 µM 0.00194 58 0.00112
20 SGTC_303 1348-1668 3.04 34.2 µM 0.00205 23 0.00119

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 9.32E-17 YDR244W PEX5 hom Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
0.125 3.59E-13 YLR354C TAL1 hom Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
0.119 3.79E-12 YIL166C_p YIL166C_p hom Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
0.103 1.86E-9 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.097 1.56E-8 YER173W RAD24 hom Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
0.097 1.65E-8 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.091 1.29E-7 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.087 3.84E-7 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.087 4.41E-7 YPL020C ULP1 het Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
0.087 4.70E-7 YNL064C YDJ1 hom Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family
0.086 5.91E-7 YKL085W MDH1 hom Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
0.086 6.96E-7 YHR043C DOG2 hom 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed
0.085 9.03E-7 YMR082C_d YMR082C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.083 1.37E-6 YJR012C_p YJR012C_p het Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
0.082 2.02E-6 YGR231C PHB2 hom Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation