YPR001W / CIT3

Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR001W / CIT3

Click on Significant Values for Screen Details ID:SGTC_339|Compound:3091-4833|FD-Score:3.58|P-value:4.08E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:5.73|P-value:3.89E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:3.66|P-value:3.07E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.80|P-value:1.91E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.61|P-value:3.64E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-3.74|P-value:2.06E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.79|P-value:1.93E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.22|P-value:3.91E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.57|P-value:4.18E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:4.12|P-value:5.69E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.54|P-value:4.70E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.70|P-value:2.69E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:4.00|P-value:9.22E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:4.25|P-value:3.40E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:4.22|P-value:3.97E-5 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.52|P-value:4.51E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:5.22|P-value:5.08E-7 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.60|P-value:8.39E-6 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.60|P-value:3.73E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.33|P-value:9.12E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.44|P-value:6.51E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.40|P-value:7.37E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.57|P-value:4.13E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.60|P-value:3.80E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.68|P-value:2.89E-4 ID:SGTC_1897|Compound:st060201|FD-Score:5.94|P-value:1.28E-8 ID:SGTC_1904|Compound:st060225|FD-Score:3.39|P-value:7.49E-4 ID:SGTC_1905|Compound:st060207|FD-Score:4.40|P-value:1.88E-5 ID:SGTC_2283|Compound:7944793|FD-Score:5.49|P-value:1.31E-7 ID:SGTC_2300|Compound:7976697|FD-Score:4.24|P-value:3.63E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.43|P-value:6.70E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.32|P-value:2.61E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.62|P-value:7.65E-6 ID:SGTC_2692|Compound:st077426|FD-Score:4.69|P-value:5.69E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.34|P-value:8.84E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.87|P-value:1.44E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.58|P-value:3.59E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.58|P-value:4.08E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:5.73|P-value:3.89E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:3.66|P-value:3.07E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.80|P-value:1.91E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.61|P-value:3.64E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-3.74|P-value:2.06E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.79|P-value:1.93E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.22|P-value:3.91E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.57|P-value:4.18E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:4.12|P-value:5.69E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.54|P-value:4.70E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.70|P-value:2.69E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:4.00|P-value:9.22E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:4.25|P-value:3.40E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:4.22|P-value:3.97E-5 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.52|P-value:4.51E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:5.22|P-value:5.08E-7 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.60|P-value:8.39E-6 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.60|P-value:3.73E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.33|P-value:9.12E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.44|P-value:6.51E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.40|P-value:7.37E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.57|P-value:4.13E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.60|P-value:3.80E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.68|P-value:2.89E-4 ID:SGTC_1897|Compound:st060201|FD-Score:5.94|P-value:1.28E-8 ID:SGTC_1904|Compound:st060225|FD-Score:3.39|P-value:7.49E-4 ID:SGTC_1905|Compound:st060207|FD-Score:4.40|P-value:1.88E-5 ID:SGTC_2283|Compound:7944793|FD-Score:5.49|P-value:1.31E-7 ID:SGTC_2300|Compound:7976697|FD-Score:4.24|P-value:3.63E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.43|P-value:6.70E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.32|P-value:2.61E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.62|P-value:7.65E-6 ID:SGTC_2692|Compound:st077426|FD-Score:4.69|P-value:5.69E-6 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.34|P-value:8.84E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.87|P-value:1.44E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.58|P-value:3.59E-4

Top fitness defect scores for YPR001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1897 st060201 5.94 15.0 µM 1.28E-8 mitochondrial processes 5 1.41E-9
2 SGTC_345 1362-0200 5.73 395.0 µM 3.89E-8 4 4.94E-9
3 SGTC_2283 7944793 5.49 200.0 µM 1.31E-7 11 1.96E-8
4 SGTC_976 1348-1067 5.22 47.6 µM 5.08E-7 60S ribosome export 7 9.04E-8
5 SGTC_2692 st077426 4.69 47.7 µM 5.69E-6 11 1.39E-6
6 SGTC_2591 skatole 4.62 100.0 µM 7.65E-6 21 1.94E-6
7 SGTC_1025 k007-0731 4.60 70.0 µM 8.39E-6 7 2.16E-6
8 SGTC_1905 st060207 4.40 84.5 µM 1.88E-5 5 5.37E-6
9 SGTC_2481 5478948 4.32 45.2 µM 2.61E-5 PDR1 26 7.77E-6
10 SGTC_850 0388-0143 4.25 75.9 µM 3.40E-5 9 1.05E-5
11 SGTC_2300 7976697 4.24 71.6 µM 3.63E-5 ERG2 26 1.13E-5
12 SGTC_601 0986-0029 4.22 62.9 µM 3.91E-5 calcium & mitochondrial duress 29 1.23E-5
13 SGTC_856 0433-0016 4.22 11.3 µM 3.97E-5 11 1.25E-5
14 SGTC_740 k014-0057 4.12 261.0 µM 5.69E-5 21 1.87E-5
15 SGTC_843 0310-0051 4.00 143.0 µM 9.22E-5 11 3.23E-5
16 SGTC_2959 9079155 3.87 11.0 µM 1.44E-4 30 5.36E-5
17 SGTC_544 k015-0020 3.80 52.4 µM 1.91E-4 14 7.35E-5
18 SGTC_593 1203-0218 3.79 61.7 µM 1.93E-4 14 7.46E-5
19 SGTC_805 3852-0218 3.70 479.0 µM 2.69E-4 ERAD & cell cycle 82 1.08E-4
20 SGTC_1892 st058411 3.68 7.4 µM 2.89E-4 32 1.17E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.096 2.24E-8 YKR058W GLG1 hom Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
0.094 5.54E-8 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.093 7.88E-8 YIL004C BET1 het Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
0.083 1.70E-6 YOR188W MSB1 hom Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.079 4.90E-6 YHR171W ATG7 hom Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation
0.077 7.40E-6 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.074 1.74E-5 YER037W PHM8 hom Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p
0.074 1.88E-5 YIL087C AIM19 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth
0.069 6.22E-5 YKR103W NFT1 hom Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
0.069 6.28E-5 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.069 6.49E-5 YNL095C_p YNL095C_p hom Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
0.068 8.37E-5 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.068 8.91E-5 YLR318W EST2 hom Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
0.067 1.09E-4 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.066 1.42E-4 YOR058C ASE1 hom Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress