YPR004C / AIM45

Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR004C / AIM45

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:4.75|P-value:7.02E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:3.80|P-value:5.77E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:3.10|P-value:8.15E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:4.45|P-value:3.11E-6 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.29|P-value:4.03E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.51|P-value:1.86E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.64|P-value:1.11E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.08|P-value:8.90E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.16|P-value:6.69E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.38|P-value:4.29E-6 ID:SGTC_971|Compound:1315-0384|FD-Score:3.95|P-value:3.04E-5 ID:SGTC_986|Compound:1493-0483|FD-Score:4.59|P-value:1.60E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.38|P-value:4.38E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.90|P-value:3.18E-7 ID:SGTC_1281|Compound:0884-0031|FD-Score:4.49|P-value:2.53E-6 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.64|P-value:1.23E-6 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.24|P-value:5.04E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.86|P-value:4.44E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.36|P-value:3.21E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.06|P-value:1.85E-5 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.27|P-value:4.54E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.05|P-value:9.68E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.26|P-value:4.64E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.71|P-value:8.49E-7 ID:SGTC_1675|Compound:st018485|FD-Score:3.15|P-value:6.89E-4 ID:SGTC_1685|Compound:st024049|FD-Score:4.24|P-value:8.39E-6 ID:SGTC_1797|Compound:st048695|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.35|P-value:3.25E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.36|P-value:3.21E-4 ID:SGTC_1961|Compound:st076550|FD-Score:-3.24|P-value:4.71E-4 ID:SGTC_1967|Compound:st070177|FD-Score:5.08|P-value:1.22E-7 ID:SGTC_2062|Compound:5245942|FD-Score:3.36|P-value:3.28E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.52|P-value:1.78E-4 ID:SGTC_2265|Compound:7929201|FD-Score:4.64|P-value:1.25E-6 ID:SGTC_2272|Compound:7805186|FD-Score:-3.13|P-value:7.06E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-3.50|P-value:1.80E-4 ID:SGTC_2279|Compound:7948423|FD-Score:-3.41|P-value:2.58E-4 ID:SGTC_2353|Compound:9059630|FD-Score:3.45|P-value:2.29E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.25|P-value:4.54E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:9.23|P-value:3.16E-21 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-4.43|P-value:3.16E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.28|P-value:6.99E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.25|P-value:4.61E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.10|P-value:8.29E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.47|P-value:2.11E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.57|P-value:1.42E-4 ID:SGTC_2846|Compound:9008642|FD-Score:5.16|P-value:7.88E-8 ID:SGTC_3204|Compound:9114183|FD-Score:3.23|P-value:5.25E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-3.46|P-value:2.12E-4 ID:SGTC_3208|Compound:9114115|FD-Score:4.72|P-value:8.24E-7 ID:SGTC_3212|Compound:9128307|FD-Score:3.46|P-value:2.20E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.43|P-value:2.44E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.33|P-value:3.63E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:4.75|P-value:7.02E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:3.80|P-value:5.77E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:3.10|P-value:8.15E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:4.45|P-value:3.11E-6 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.29|P-value:4.03E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.51|P-value:1.86E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.64|P-value:1.11E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.08|P-value:8.90E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.16|P-value:6.69E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.38|P-value:4.29E-6 ID:SGTC_971|Compound:1315-0384|FD-Score:3.95|P-value:3.04E-5 ID:SGTC_986|Compound:1493-0483|FD-Score:4.59|P-value:1.60E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.38|P-value:4.38E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.90|P-value:3.18E-7 ID:SGTC_1281|Compound:0884-0031|FD-Score:4.49|P-value:2.53E-6 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.64|P-value:1.23E-6 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.24|P-value:5.04E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.86|P-value:4.44E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.36|P-value:3.21E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.06|P-value:1.85E-5 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.27|P-value:4.54E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.05|P-value:9.68E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.26|P-value:4.64E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.71|P-value:8.49E-7 ID:SGTC_1675|Compound:st018485|FD-Score:3.15|P-value:6.89E-4 ID:SGTC_1685|Compound:st024049|FD-Score:4.24|P-value:8.39E-6 ID:SGTC_1797|Compound:st048695|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.35|P-value:3.25E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.36|P-value:3.21E-4 ID:SGTC_1961|Compound:st076550|FD-Score:-3.24|P-value:4.71E-4 ID:SGTC_1967|Compound:st070177|FD-Score:5.08|P-value:1.22E-7 ID:SGTC_2062|Compound:5245942|FD-Score:3.36|P-value:3.28E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.52|P-value:1.78E-4 ID:SGTC_2265|Compound:7929201|FD-Score:4.64|P-value:1.25E-6 ID:SGTC_2272|Compound:7805186|FD-Score:-3.13|P-value:7.06E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-3.50|P-value:1.80E-4 ID:SGTC_2279|Compound:7948423|FD-Score:-3.41|P-value:2.58E-4 ID:SGTC_2353|Compound:9059630|FD-Score:3.45|P-value:2.29E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.25|P-value:4.54E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:9.23|P-value:3.16E-21 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-4.43|P-value:3.16E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.28|P-value:6.99E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.25|P-value:4.61E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.10|P-value:8.29E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.47|P-value:2.11E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.57|P-value:1.42E-4 ID:SGTC_2846|Compound:9008642|FD-Score:5.16|P-value:7.88E-8 ID:SGTC_3204|Compound:9114183|FD-Score:3.23|P-value:5.25E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-3.46|P-value:2.12E-4 ID:SGTC_3208|Compound:9114115|FD-Score:4.72|P-value:8.24E-7 ID:SGTC_3212|Compound:9128307|FD-Score:3.46|P-value:2.20E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.43|P-value:2.44E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.33|P-value:3.63E-4

Top fitness defect scores for YPR004C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2567 aureobasidin a 9.23 60.0 nM 3.16E-21 15 1.33E-20
2 SGTC_2846 9008642 5.16 19.5 µM 7.88E-8 8 1.23E-7
3 SGTC_1967 st070177 5.08 51.4 µM 1.22E-7 5 1.88E-7
4 SGTC_1261 0712-0010 4.90 12.7 µM 3.18E-7 RNA processing & uracil transport 10 4.74E-7
5 SGTC_39 0986-0283 4.75 58.4 µM 7.02E-7 calcium & mitochondrial duress 15 1.02E-6
6 SGTC_3208 9114115 4.72 49.5 µM 8.24E-7 20 1.19E-6
7 SGTC_1655 st012546 4.71 55.1 µM 8.49E-7 12 1.23E-6
8 SGTC_1355 1499-0035 4.64 1.7 µM 1.23E-6 6 1.76E-6
9 SGTC_2265 7929201 4.64 200.0 µM 1.25E-6 15 1.78E-6
10 SGTC_986 1493-0483 4.59 84.0 µM 1.60E-6 7 2.26E-6
11 SGTC_1281 0884-0031 4.49 11.8 µM 2.53E-6 fatty acid desaturase (OLE1) 15 3.53E-6
12 SGTC_107 0358-0022 4.45 2.7 µM 3.11E-6 SWF1 & branched chain AA biosynthesis 22 4.32E-6
13 SGTC_927 2425-1185 4.38 123.0 µM 4.29E-6 20 5.89E-6
14 SGTC_1253 0550-0034 4.38 176.0 µM 4.38E-6 12 6.01E-6
15 SGTC_2583 asiatic acid 4.28 100.0 µM 6.99E-6 29 9.45E-6
16 SGTC_1685 st024049 4.24 53.1 µM 8.39E-6 TSC3-RPN4 9 1.13E-5
17 SGTC_1543 azetidyl-2-carboxylic acid 4.06 198.0 µM 1.85E-5 11 2.43E-5
18 SGTC_971 1315-0384 3.95 26.7 µM 3.04E-5 cell wall signaling 23 3.93E-5
19 SGTC_1369 1866-0067 3.86 208.0 µM 4.44E-5 RPP1 & pyrimidine depletion 41 5.68E-5
20 SGTC_44 0929-0055 3.80 61.2 µM 5.77E-5 36 7.32E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.299 4.36E-70 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.181 3.55E-26 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.144 4.57E-17 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.133 9.33E-15 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.130 4.84E-14 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.127 1.87E-13 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.117 1.04E-11 YNL318C HXT14 hom Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.112 7.27E-11 YDR224C HTB1 het Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.110 2.02E-10 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.102 3.74E-9 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.095 3.13E-8 YCR069W CPR4 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
0.092 1.06E-7 YJR003C_p YJR003C_p hom Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis
0.091 1.42E-7 YPL220W RPL1A hom Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.090 1.99E-7 YEL011W GLC3 hom Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.085 9.53E-7 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities