YPR019W / MCM4

Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR019W / MCM4

Click on Significant Values for Screen Details ID:SGTC_119|Compound:0121-0043|FD-Score:2.68|P-value:8.09E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.40|P-value:3.13E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:-2.71|P-value:6.98E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.98|P-value:1.40E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.20|P-value:8.00E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:-2.75|P-value:6.09E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.16|P-value:9.89E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:2.96|P-value:2.46E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.80|P-value:4.86E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:5.36|P-value:1.39E-10 ID:SGTC_656|Compound:4090-1979|FD-Score:2.92|P-value:2.97E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.80|P-value:4.82E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:2.71|P-value:7.12E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.22|P-value:7.46E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:4.20|P-value:3.72E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:2.76|P-value:5.85E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:2.90|P-value:3.20E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.81|P-value:3.58E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:6.24|P-value:1.02E-13 ID:SGTC_1134|Compound:3477-0009|FD-Score:-2.76|P-value:5.69E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:2.70|P-value:7.42E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.50|P-value:1.91E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.84|P-value:4.03E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.99|P-value:2.12E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:-3.02|P-value:1.89E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.19|P-value:8.75E-5 ID:SGTC_1658|Compound:st012944|FD-Score:-3.05|P-value:1.67E-4 ID:SGTC_1674|Compound:st016616|FD-Score:2.83|P-value:4.29E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.32|P-value:4.55E-5 ID:SGTC_1982|Compound:st072467|FD-Score:2.65|P-value:8.90E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.32|P-value:4.55E-5 ID:SGTC_2098|Compound:5376439|FD-Score:-2.82|P-value:4.57E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.66|P-value:8.23E-6 ID:SGTC_2276|Compound:7947513|FD-Score:-3.50|P-value:1.90E-5 ID:SGTC_2279|Compound:7948423|FD-Score:2.99|P-value:2.18E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.73|P-value:6.44E-4 ID:SGTC_2309|Compound:7664088|FD-Score:6.75|P-value:9.66E-16 ID:SGTC_2409|Compound:alverine citrate|FD-Score:2.69|P-value:7.64E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:2.92|P-value:2.85E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.24|P-value:6.80E-5 ID:SGTC_2483|Compound:5729050|FD-Score:4.67|P-value:1.87E-8 ID:SGTC_2484|Compound:5809818|FD-Score:-3.21|P-value:7.67E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.55|P-value:3.04E-11 ID:SGTC_2526|Compound:patulin|FD-Score:2.79|P-value:5.13E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:2.87|P-value:3.64E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-2.63|P-value:9.78E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.75|P-value:5.06E-6 ID:SGTC_2804|Compound:7954240|FD-Score:4.61|P-value:2.74E-8 ID:SGTC_2850|Compound:9015429|FD-Score:-2.79|P-value:5.15E-4 ID:SGTC_2969|Compound:9091408|FD-Score:2.77|P-value:5.48E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-2.79|P-value:5.17E-4 ID:SGTC_2977|Compound:9011197|FD-Score:3.67|P-value:7.58E-6 ID:SGTC_2981|Compound:9013952|FD-Score:3.54|P-value:1.56E-5 ID:SGTC_3208|Compound:9114115|FD-Score:2.70|P-value:7.23E-4 ID:SGTC_3311|Compound:9131146|FD-Score:2.68|P-value:7.95E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:2.68|P-value:8.09E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.40|P-value:3.13E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:-2.71|P-value:6.98E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.98|P-value:1.40E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.20|P-value:8.00E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:-2.75|P-value:6.09E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.16|P-value:9.89E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:2.96|P-value:2.46E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.80|P-value:4.86E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:5.36|P-value:1.39E-10 ID:SGTC_656|Compound:4090-1979|FD-Score:2.92|P-value:2.97E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.80|P-value:4.82E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:2.71|P-value:7.12E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.22|P-value:7.46E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:4.20|P-value:3.72E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:2.76|P-value:5.85E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:2.90|P-value:3.20E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.81|P-value:3.58E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:6.24|P-value:1.02E-13 ID:SGTC_1134|Compound:3477-0009|FD-Score:-2.76|P-value:5.69E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:2.70|P-value:7.42E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.50|P-value:1.91E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:2.84|P-value:4.03E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.99|P-value:2.12E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:-3.02|P-value:1.89E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.19|P-value:8.75E-5 ID:SGTC_1658|Compound:st012944|FD-Score:-3.05|P-value:1.67E-4 ID:SGTC_1674|Compound:st016616|FD-Score:2.83|P-value:4.29E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.32|P-value:4.55E-5 ID:SGTC_1982|Compound:st072467|FD-Score:2.65|P-value:8.90E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.32|P-value:4.55E-5 ID:SGTC_2098|Compound:5376439|FD-Score:-2.82|P-value:4.57E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.66|P-value:8.23E-6 ID:SGTC_2276|Compound:7947513|FD-Score:-3.50|P-value:1.90E-5 ID:SGTC_2279|Compound:7948423|FD-Score:2.99|P-value:2.18E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.73|P-value:6.44E-4 ID:SGTC_2309|Compound:7664088|FD-Score:6.75|P-value:9.66E-16 ID:SGTC_2409|Compound:alverine citrate|FD-Score:2.69|P-value:7.64E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:2.92|P-value:2.85E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.24|P-value:6.80E-5 ID:SGTC_2483|Compound:5729050|FD-Score:4.67|P-value:1.87E-8 ID:SGTC_2484|Compound:5809818|FD-Score:-3.21|P-value:7.67E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.55|P-value:3.04E-11 ID:SGTC_2526|Compound:patulin|FD-Score:2.79|P-value:5.13E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:2.87|P-value:3.64E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-2.63|P-value:9.78E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.75|P-value:5.06E-6 ID:SGTC_2804|Compound:7954240|FD-Score:4.61|P-value:2.74E-8 ID:SGTC_2850|Compound:9015429|FD-Score:-2.79|P-value:5.15E-4 ID:SGTC_2969|Compound:9091408|FD-Score:2.77|P-value:5.48E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-2.79|P-value:5.17E-4 ID:SGTC_2977|Compound:9011197|FD-Score:3.67|P-value:7.58E-6 ID:SGTC_2981|Compound:9013952|FD-Score:3.54|P-value:1.56E-5 ID:SGTC_3208|Compound:9114115|FD-Score:2.70|P-value:7.23E-4 ID:SGTC_3311|Compound:9131146|FD-Score:2.68|P-value:7.95E-4

Top fitness defect scores for YPR019W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2309 7664088 6.75 163.9 µM 9.66E-16 3 7.61E-12 1.36
2 SGTC_1082 camptothecin 6.24 424.9 nM 1.02E-13 DNA damage response 5 2.23E-10 0.26
3 SGTC_2522 biochanin a 5.55 4.9 µM 3.04E-11 RNA pol III & RNase P/MRP 6 1.40E-8 0.03
4 SGTC_627 1391-0720 5.36 175.0 µM 1.39E-10 1 4.21E-8 1.18
5 SGTC_2483 5729050 4.67 27.2 µM 1.87E-8 3 1.50E-6 0.09
6 SGTC_2804 7954240 4.61 19.5 µM 2.74E-8 8 1.97E-6 0.46
7 SGTC_933 2914-0560 4.20 114.0 µM 3.72E-7 4 1.32E-5 0.33
8 SGTC_228 caffeine 3.98 993.1 µM 1.40E-6 3 3.47E-5 0.05
9 SGTC_1056 3448-1962 3.81 115.0 µM 3.58E-6 amide catabolism 8 6.93E-5 0.02
10 SGTC_2777 climbazole 3.75 1.5 µM 5.06E-6 iron homeostasis 6 8.91E-5 0.24
11 SGTC_2977 9011197 3.67 71.4 µM 7.58E-6 12 1.20E-4 0.08
12 SGTC_2275 7947463 3.66 200.0 µM 8.23E-6 iron homeostasis 17 1.27E-4 0.41
13 SGTC_2981 9013952 3.54 39.0 µM 1.56E-5 13 2.03E-4 0.00
14 SGTC_1312 1218-2183 3.50 165.0 µM 1.91E-5 3 2.36E-4 0.23
15 SGTC_1714 st033236 3.32 13.6 µM 4.55E-5 14 4.47E-4 0.10
16 SGTC_2442 5158906 3.24 48.5 µM 6.80E-5 5 6.00E-4 0.06
17 SGTC_236 1509-0011 3.20 5.3 µM 8.00E-5 41 6.76E-4 0.03
18 SGTC_1653 st012112 3.19 65.0 µM 8.75E-5 7 7.22E-4 0.01
19 SGTC_514 4466-0038 3.16 2.6 nM 9.89E-5 cell wall 55 7.89E-4 0.03
20 SGTC_2279 7948423 2.99 32.9 µM 2.18E-4 16 0.00141 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.28E-17 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.142 1.32E-16 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.128 1.15E-13 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.127 1.81E-13 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.124 6.84E-13 YML054C-A_p YML054C-A_p hom Putative protein of unknown function
0.119 5.05E-12 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.118 5.88E-12 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.116 1.39E-11 YBR085C-A YBR085C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
0.114 3.79E-11 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.112 6.57E-11 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.112 6.87E-11 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.112 8.01E-11 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.111 1.18E-10 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.111 1.24E-10 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.109 2.31E-10 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p